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The map label for this gene is nadE [H]

Identifier: 17547066

GI number: 17547066

Start: 2544809

End: 2546449

Strand: Reverse

Name: nadE [H]

Synonym: RSc2347

Alternate gene names: 17547066

Gene position: 2546449-2544809 (Counterclockwise)

Preceding gene: 17547068

Following gene: 17547065

Centisome position: 68.52

GC content: 68.13

Gene sequence:

>1641_bases
ATGACCGTTCTCGTCGCTCTTGCCCAGATCAACTGCACCGTCGGCGATCTTGCCGGCAACGCCGCCCGCATCGTCGCGGC
GGCCCGCGAGGCGCACCAGGCTGGTGCGCGCATTCTTGTCACGCCCGAACTGTCGCTGACCGGCTATCCGCCCGAAGACC
TGCTGCTGCGTCCGGCCTTCCTGGATGCCGCGTCACGCGCGCTGGATGCGCTGGTGGCCGAGCTGGCCGCTTTCGCGGGC
CTGCATGTGCTGATCGGCCATCCGCAGCTGTTGTTCGAGGCGCCGGCGCCGGGCAGCGCGCTGCCCAAGCCGACCAACAC
CGCCACCGTGGCGGTCGACGGCCGCGTGCTCGGCCAATATCACAAGCTCGAGCTGCCCAACAACGAGGTCTTCGACGAGA
AGCGCTACTTCCAGGTCGGCGGCGAACCCTTCGTGTTCGACGTGGAGGGCACGCGCTTCGGCGTGATCCTCTGCGAGGAC
GCGTGGTATCCCAAGGCCACGGCCTGGGCCAAGGCGGCGGGCGCGCAGGTGGTGCTGATCCCCAACGCGTCGCCGTATCA
CCTGGACAAGGAAGACCTGCGCGAGCAGATCATCGGCGCGCGGGTGCAGGAGTCGGGCCTGCCGCATGTCTATGTGAACC
TCGTCGGCGGACAGGACGAGCTGGTGTTCGACGGCGGCTCCTTCGTGCTGGATGCCCACGGCAAGCCGGCGGCGCGGATG
GCGCAGTTCGTCGAGGGCGTGGGCTACGTGCGCTTCGACGGCGCCAGGCCCCTGCCCGGCGAGATCGCCCCGGAGGCGAC
GCTGGAGGCGCAGGTGTACGCGGCGCTCAAGCTGGGCGTGCGCGACTACATCGGCAAGAACGGCTTCCCTGGGGCGATCA
TCGGGCTGTCGGGCGGCGTCGACTCGGCGCTGGTGCTGGCGATCGCCGTCGATGCGCTGGGCGCCGACCGCGTGCGCGCG
GTGATGATGCCGTCGCGCTACACGGCCGACATCTCGTGGCTCGACGCGCGCGACATGGCCGCACGCCTGGGCGTGCGGTA
CGACGAGCTCCCGATCATGCCGATGTTCGACGCGTTCAGGGGCGCGCTGGCGGAAGAGTTTCGCGACCTGCCCGAAGACG
CCACCGAAGAGAACATCCAGGCGCGCATCCGCGGCACGCTGCTGATGGCGCTGTCCAACAAGTCCGGGCGCATCGTGCTG
ACCACCGGCAACAAGAGCGAGATGGCGGTTGGCTACTGCACGCTGTACGGCGACATGGCGGGCGGCTTCGCGGTCATCAA
GGACATCTTCAAGACGCTGGTCTACCGGCTGTGCCGCTATCGCAATGCGCTGTCGGAGGTGATTCCCGAGCGCATCCTCA
CGCGCGCGCCGTCGGCCGAACTGCGCGAGAACCAGACCGACCAGGACAGCCTGCCCGAATACGACGCGCTCGATGCCATC
GTGCAGCGCTACATGGAGCAGAACCAGCCGGTGGCCGAGATCATCGCCGCGGGGTTTGCCGAGGCCGATGTGCAGAAGAT
CGTGCGGCTCATCAAGATCAACGAATACAAGCGCCGCCAGGCGCCGGTGGGCATCCGCGTGACGCAGCGGGCATTCGGCC
GGGACTGGCGGTATCCGATCACCTCACGCTTCAAAGAGTAG

Upstream 100 bases:

>100_bases
TCCGGCGCGGGTTTCGGGCTCGGGAACGAGAGGGCGGGAGGTCATGCCGCCGATTCTAATTCCTGCCCGGGACGCCGTGC
CCGCTCCGACATCGCCTTCC

Downstream 100 bases:

>100_bases
GCCGGCCACGCGATGCTCGACTACTACGCCAAGCGCGCTCCCGAATACGAGCGCATCTACGCAAAGCCGGAACGGCAGGC
GGACTTGGCGTGGCTGAGGG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MTVLVALAQINCTVGDLAGNAARIVAAAREAHQAGARILVTPELSLTGYPPEDLLLRPAFLDAASRALDALVAELAAFAG
LHVLIGHPQLLFEAPAPGSALPKPTNTATVAVDGRVLGQYHKLELPNNEVFDEKRYFQVGGEPFVFDVEGTRFGVILCED
AWYPKATAWAKAAGAQVVLIPNASPYHLDKEDLREQIIGARVQESGLPHVYVNLVGGQDELVFDGGSFVLDAHGKPAARM
AQFVEGVGYVRFDGARPLPGEIAPEATLEAQVYAALKLGVRDYIGKNGFPGAIIGLSGGVDSALVLAIAVDALGADRVRA
VMMPSRYTADISWLDARDMAARLGVRYDELPIMPMFDAFRGALAEEFRDLPEDATEENIQARIRGTLLMALSNKSGRIVL
TTGNKSEMAVGYCTLYGDMAGGFAVIKDIFKTLVYRLCRYRNALSEVIPERILTRAPSAELRENQTDQDSLPEYDALDAI
VQRYMEQNQPVAEIIAAGFAEADVQKIVRLIKINEYKRRQAPVGIRVTQRAFGRDWRYPITSRFKE

Sequences:

>Translated_546_residues
MTVLVALAQINCTVGDLAGNAARIVAAAREAHQAGARILVTPELSLTGYPPEDLLLRPAFLDAASRALDALVAELAAFAG
LHVLIGHPQLLFEAPAPGSALPKPTNTATVAVDGRVLGQYHKLELPNNEVFDEKRYFQVGGEPFVFDVEGTRFGVILCED
AWYPKATAWAKAAGAQVVLIPNASPYHLDKEDLREQIIGARVQESGLPHVYVNLVGGQDELVFDGGSFVLDAHGKPAARM
AQFVEGVGYVRFDGARPLPGEIAPEATLEAQVYAALKLGVRDYIGKNGFPGAIIGLSGGVDSALVLAIAVDALGADRVRA
VMMPSRYTADISWLDARDMAARLGVRYDELPIMPMFDAFRGALAEEFRDLPEDATEENIQARIRGTLLMALSNKSGRIVL
TTGNKSEMAVGYCTLYGDMAGGFAVIKDIFKTLVYRLCRYRNALSEVIPERILTRAPSAELRENQTDQDSLPEYDALDAI
VQRYMEQNQPVAEIIAAGFAEADVQKIVRLIKINEYKRRQAPVGIRVTQRAFGRDWRYPITSRFKE
>Mature_545_residues
TVLVALAQINCTVGDLAGNAARIVAAAREAHQAGARILVTPELSLTGYPPEDLLLRPAFLDAASRALDALVAELAAFAGL
HVLIGHPQLLFEAPAPGSALPKPTNTATVAVDGRVLGQYHKLELPNNEVFDEKRYFQVGGEPFVFDVEGTRFGVILCEDA
WYPKATAWAKAAGAQVVLIPNASPYHLDKEDLREQIIGARVQESGLPHVYVNLVGGQDELVFDGGSFVLDAHGKPAARMA
QFVEGVGYVRFDGARPLPGEIAPEATLEAQVYAALKLGVRDYIGKNGFPGAIIGLSGGVDSALVLAIAVDALGADRVRAV
MMPSRYTADISWLDARDMAARLGVRYDELPIMPMFDAFRGALAEEFRDLPEDATEENIQARIRGTLLMALSNKSGRIVLT
TGNKSEMAVGYCTLYGDMAGGFAVIKDIFKTLVYRLCRYRNALSEVIPERILTRAPSAELRENQTDQDSLPEYDALDAIV
QRYMEQNQPVAEIIAAGFAEADVQKIVRLIKINEYKRRQAPVGIRVTQRAFGRDWRYPITSRFKE

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 59483; Mature: 59352

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLVALAQINCTVGDLAGNAARIVAAAREAHQAGARILVTPELSLTGYPPEDLLLRPAF
CEEEEEEEECCCCHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCEECCCCCHHHEECHHH
LDAASRALDALVAELAAFAGLHVLIGHPQLLFEAPAPGSALPKPTNTATVAVDGRVLGQY
HHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCCCCCEEEEECCHHHCCE
HKLELPNNEVFDEKRYFQVGGEPFVFDVEGTRFGVILCEDAWYPKATAWAKAAGAQVVLI
EEEECCCCCCCCCCHHEEECCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHCCCEEEEE
PNASPYHLDKEDLREQIIGARVQESGLPHVYVNLVGGQDELVFDGGSFVLDAHGKPAARM
CCCCCCCCCHHHHHHHHHCCHHHHCCCCEEEEEEECCCCEEEEECCCEEEECCCCCHHHH
AQFVEGVGYVRFDGARPLPGEIAPEATLEAQVYAALKLGVRDYIGKNGFPGAIIGLSGGV
HHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
DSALVLAIAVDALGADRVRAVMMPSRYTADISWLDARDMAARLGVRYDELPIMPMFDAFR
CHHHHHHHHHHHCCCHHEEEEECCCCCCCCEEECCHHHHHHHHCCCCCCCCCCHHHHHHH
GALAEEFRDLPEDATEENIQARIRGTLLMALSNKSGRIVLTTGNKSEMAVGYCTLYGDMA
HHHHHHHHCCCCCCHHHHHHHHHEEEEEEEEECCCCEEEEEECCCCCEEEEEEEEECCCC
GGFAVIKDIFKTLVYRLCRYRNALSEVIPERILTRAPSAELRENQTDQDSLPEYDALDAI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHH
VQRYMEQNQPVAEIIAAGFAEADVQKIVRLIKINEYKRRQAPVGIRVTQRAFGRDWRYPI
HHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCCCEEEEHHHHCCCCCCCH
TSRFKE
HHCCCC
>Mature Secondary Structure 
TVLVALAQINCTVGDLAGNAARIVAAAREAHQAGARILVTPELSLTGYPPEDLLLRPAF
EEEEEEEECCCCHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCEECCCCCHHHEECHHH
LDAASRALDALVAELAAFAGLHVLIGHPQLLFEAPAPGSALPKPTNTATVAVDGRVLGQY
HHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCCCCCEEEEECCHHHCCE
HKLELPNNEVFDEKRYFQVGGEPFVFDVEGTRFGVILCEDAWYPKATAWAKAAGAQVVLI
EEEECCCCCCCCCCHHEEECCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHCCCEEEEE
PNASPYHLDKEDLREQIIGARVQESGLPHVYVNLVGGQDELVFDGGSFVLDAHGKPAARM
CCCCCCCCCHHHHHHHHHCCHHHHCCCCEEEEEEECCCCEEEEECCCEEEECCCCCHHHH
AQFVEGVGYVRFDGARPLPGEIAPEATLEAQVYAALKLGVRDYIGKNGFPGAIIGLSGGV
HHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
DSALVLAIAVDALGADRVRAVMMPSRYTADISWLDARDMAARLGVRYDELPIMPMFDAFR
CHHHHHHHHHHHCCCHHEEEEECCCCCCCCEEECCHHHHHHHHCCCCCCCCCCHHHHHHH
GALAEEFRDLPEDATEENIQARIRGTLLMALSNKSGRIVLTTGNKSEMAVGYCTLYGDMA
HHHHHHHHCCCCCCHHHHHHHHHEEEEEEEEECCCCEEEEEECCCCCEEEEEEEEECCCC
GGFAVIKDIFKTLVYRLCRYRNALSEVIPERILTRAPSAELRENQTDQDSLPEYDALDAI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHH
VQRYMEQNQPVAEIIAAGFAEADVQKIVRLIKINEYKRRQAPVGIRVTQRAFGRDWRYPI
HHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCCCEEEEHHHHCCCCCCCH
TSRFKE
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]