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The map label for this gene is tISRso6b [H]

Identifier: 17547053

GI number: 17547053

Start: 2532717

End: 2533562

Strand: Direct

Name: tISRso6b [H]

Synonym: RSc2334

Alternate gene names: 17547053

Gene position: 2532717-2533562 (Clockwise)

Preceding gene: 17547052

Following gene: 17547061

Centisome position: 68.15

GC content: 61.82

Gene sequence:

>846_bases
ATGACGCCTCAGCTCGAACGGCTCACCGTCCTGTGCCAGCAGCTCAACCTGCAGCACCTTCCCACACAGCTCGCCCACCT
GGGCCAGATGGCGGCCAAGCGGGAGCTCGGCTACCTGGACTTCCTTGAGCACGCCTTGAAGGACGAAGCCATGGCGCGGG
CCGAGCGCATGCGACGAACGCTGACACGATTGGCCGGCTTCCCGGCGATCAAGACCTTGGACGAGTTCGACTTCGAGTTC
GCAACCGGCGTGCCGAAGGCGATGGTCCTGGAGTTGGGCAGCCTTGCATTCGTTGAGCGAGCCGAGAACGTGGTGCTGCT
CGGACCTTCCGGCGTGGGGAAGACCCACCTGGCGATCGCCCTGGGCTATCGCGCCACGCAGGCCGGCATGCGGACGCGGT
TCATCACGGCGGCCGATCTGCTGATGCAACTTGCAGCCGCCCTGCAGCGGAACCAGCTCGATGTCGCCATCAAGCGCATT
GTGAATCCCTACAAGCTCTTGATCGTTGACGAGATGGGCTACCTGCCGATGGACCGAGAACAAGCCAATCTACTGTTCCA
GGTCATTGCTAAGCGGTATGAAACCGGAAGCTTGGTGCTGACGAGCAATCTGCCCTTCGGTCAATGGGATCAGACCTTTG
CTGGCGACGCCACGCTCACTGCCGCTCTGCTAGATCGCCTGCTGCATCACGCGCACGTTGTGCCGATCTCCGGCGACTCG
TACCGACTGAAGGAGAAGCGCCAGGCGGGCATGATCCATCGAATGTCGCTGTCGAATCCTGTGCCCGACGACCTCAAACG
CAAACGCATTCCAAAGGAGCCACCGCCCAGCCACCCGATGCAATAA

Upstream 100 bases:

>100_bases
CGCCCAAGCCGGCCTCCAGGTTTGCGACGCCGGACTGGCATGTCTGCTTGCAGCACCCGCTGTCCGTCTACGATTCCTTC
TGCCAGCAGGGAGTCGCCGC

Downstream 100 bases:

>100_bases
CCATCAGAGGGGGTGTATCAATTTACAAATGCGTTTGCACCTGAATCTGTATCAACTTGAGACTGCGTTTGACAGCGCCA
CCATCGAGACCGTCTTGCCC

Product: transposase/IS protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MTPQLERLTVLCQQLNLQHLPTQLAHLGQMAAKRELGYLDFLEHALKDEAMARAERMRRTLTRLAGFPAIKTLDEFDFEF
ATGVPKAMVLELGSLAFVERAENVVLLGPSGVGKTHLAIALGYRATQAGMRTRFITAADLLMQLAAALQRNQLDVAIKRI
VNPYKLLIVDEMGYLPMDREQANLLFQVIAKRYETGSLVLTSNLPFGQWDQTFAGDATLTAALLDRLLHHAHVVPISGDS
YRLKEKRQAGMIHRMSLSNPVPDDLKRKRIPKEPPPSHPMQ

Sequences:

>Translated_281_residues
MTPQLERLTVLCQQLNLQHLPTQLAHLGQMAAKRELGYLDFLEHALKDEAMARAERMRRTLTRLAGFPAIKTLDEFDFEF
ATGVPKAMVLELGSLAFVERAENVVLLGPSGVGKTHLAIALGYRATQAGMRTRFITAADLLMQLAAALQRNQLDVAIKRI
VNPYKLLIVDEMGYLPMDREQANLLFQVIAKRYETGSLVLTSNLPFGQWDQTFAGDATLTAALLDRLLHHAHVVPISGDS
YRLKEKRQAGMIHRMSLSNPVPDDLKRKRIPKEPPPSHPMQ
>Mature_280_residues
TPQLERLTVLCQQLNLQHLPTQLAHLGQMAAKRELGYLDFLEHALKDEAMARAERMRRTLTRLAGFPAIKTLDEFDFEFA
TGVPKAMVLELGSLAFVERAENVVLLGPSGVGKTHLAIALGYRATQAGMRTRFITAADLLMQLAAALQRNQLDVAIKRIV
NPYKLLIVDEMGYLPMDREQANLLFQVIAKRYETGSLVLTSNLPFGQWDQTFAGDATLTAALLDRLLHHAHVVPISGDSY
RLKEKRQAGMIHRMSLSNPVPDDLKRKRIPKEPPPSHPMQ

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 31466; Mature: 31335

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQLERLTVLCQQLNLQHLPTQLAHLGQMAAKRELGYLDFLEHALKDEAMARAERMRRT
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LTRLAGFPAIKTLDEFDFEFATGVPKAMVLELGSLAFVERAENVVLLGPSGVGKTHLAIA
HHHHHCCCHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHEEEE
LGYRATQAGMRTRFITAADLLMQLAAALQRNQLDVAIKRIVNPYKLLIVDEMGYLPMDRE
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCCCCCHH
QANLLFQVIAKRYETGSLVLTSNLPFGQWDQTFAGDATLTAALLDRLLHHAHVVPISGDS
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCC
YRLKEKRQAGMIHRMSLSNPVPDDLKRKRIPKEPPPSHPMQ
HHHHHHHHHCCCHHHHCCCCCCHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TPQLERLTVLCQQLNLQHLPTQLAHLGQMAAKRELGYLDFLEHALKDEAMARAERMRRT
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LTRLAGFPAIKTLDEFDFEFATGVPKAMVLELGSLAFVERAENVVLLGPSGVGKTHLAIA
HHHHHCCCHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHEEEE
LGYRATQAGMRTRFITAADLLMQLAAALQRNQLDVAIKRIVNPYKLLIVDEMGYLPMDRE
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCCCCCHH
QANLLFQVIAKRYETGSLVLTSNLPFGQWDQTFAGDATLTAALLDRLLHHAHVVPISGDS
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCC
YRLKEKRQAGMIHRMSLSNPVPDDLKRKRIPKEPPPSHPMQ
HHHHHHHHHCCCHHHHCCCCCCHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]