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The map label for this gene is dnaG [H]

Identifier: 17546935

GI number: 17546935

Start: 2402619

End: 2404430

Strand: Reverse

Name: dnaG [H]

Synonym: RSc2216

Alternate gene names: 17546935

Gene position: 2404430-2402619 (Counterclockwise)

Preceding gene: 17546936

Following gene: 17546934

Centisome position: 64.7

GC content: 69.21

Gene sequence:

>1812_bases
GTGATTCCGCAGCCGTTCATCGACGATCTGCTCAACCGCGTCGACATCGTCGACGTGGTGGGCCGCTACGTGCAGCTCAA
GAAGGGCGGCGCCAACTTCATGGGGCTGTGCCCGTTCCATAACGAGAAGTCGCCGTCGTTCACGGTGTCGCCGACCAAGC
AGTTCTATCACTGCTTCGGTTGCGGCGCGCACGGCTCGGCAATCGGCTTCCTGATGGAGTTCTCCGGGCTGTCGTACGTC
GAGGCCATCCGCGATCTGGCGCAGTCGGTCGGCATGGCGGTGCCGGAGGAGCGCGGCGGCCTGCCGCCCGCCGCCCGCGC
CGAGCGGCAGGCCAAGACCGTCGCCCTGGGCGACGTCATGGCGCGCGCCAGCGACCACTACCGCAAGCAGCTGCGCGGCG
CCCCCGGCGCCGTCCAGTACCTCAAGGGCCGCGGCCTGACCGGTGAGATCGCCGGGCACTTCGGCCTGGGCTACGCGCCG
GACGACTGGCAGTCGCTCGAGTCCGTGTTCGGCGCCTATCGCGACGACGCCATCGCCACACCGCTGATCGAGGCCGGCCT
GGTCATCGAAAGCGAGAAGACCGACGCCGACGGCCGGCATCGCCGCTACGACCGCTTCCGCGATCGCATCATGTTCCCCA
TCCGCAATACCAAGGGGGTGGTGATCGGCTTCGGCGGGCGGGTGCTGGGGCAGGGCGAGCCGAAATACCTGAATTCTCCC
GAGACGCCGCTGTTCAGCAAGGGCACCGAGCTGTACGGCCTGTTCGAGGCGCGCAATGCCATCCGCGAGTTCGGCTACGT
GCTGGTCGTGGAAGGCTACATGGATGTGGTGGCGCTGGCGCAGCTCGGGTTTGCCAATGCCGTGGCCACGCTGGGCACGG
CCTGCACGCCCGTGCACGTGCAGAAGCTGCTGCGGCAGGTGGATACCGTCATCTTCTCGTTCGATGGCGATGCGGCCGGG
CGCCGGGCGGCGCGGCGCGCGCTGGAAGCCTGCCTGCCGCACGTCACCGACAACAAGATCGTCAAGTTCCTATTCCTGCC
GCCCGAGCACGATCCGGATACCTTCATCCGCGAAGAGGGCACCGAAGCGTTCGCCGCCGAGGTGCGCAATGCGATGCCGC
TGTCGCGCTTCCTGCTGCAGGCGGTGACCGAAGACCTCGACCTGCGCCAGCCCGAGGGGCGCGCGCGGGCGCAGTATGAG
GCCAAGCCGCTGCTGTCGGCCATGCCGCCGGGCGGCCTGCGCCTGCAGATCGTGCGCGGGCTGGCCGAGATGACCGGCAC
CACGCCGGCCGACATCGAGGCGCTGTGCGGCCTGCGCAGCGACCCGGCGCAGGCCGGCCGCATGACCCAGAAGCGGCCGC
GTGTGGCGCGTACGGCACCGACCGCACTGGAACAGAAGGTGCTGCGTCTGCTGATGCGCTACCCCGCGCTGGCGGCCCGC
CTGGACGCCGACACGCGCACCCAGTTGACTGCGCCGGAACTGCCCCAAGGTGAGGTGTTATCGGGGTTGCTGGCCGAATG
CGACACCGCAGGCCCGGAAATGCACTTCGGCGCCTTCGTCGAGCGCCTGGGCCAGACGCCGTATGCCGAGGCCTTTGCCG
GGCTGCGCGTGGCCGTGCTGAACGACGACATCGAGCTCGAGCCGGCCACGCAGGAATTCGATGCCGCCGTGCAGAAGATG
CTGGCCGAGCCACTGCGGCGCGAACTGGAGATGCTGCAGCGCAAGATGGAAGGCGGCCACGCCACCGACGCCGACAAGGA
ACGCCTGCGATGGCTGGTCAGCGAGATCAGCCGCCGCCGCCGGCTGGTGTAG

Upstream 100 bases:

>100_bases
GGCCGCGCCGACATGACGCAGGTCTCGGCCCGCGTGAAGGCCGTGCTGGCCGGCGCCTGAGTCCGGCCGCGTTCCGTCAT
CCGCAAGGCGCGCCCCACGC

Downstream 100 bases:

>100_bases
CGCGCACGCCGCAGTGCAGGCCCAGACAGGACGGGCGATTGCGGGTAGTCCCTTGATTTTTCAGGCGAAAACCCGATTTT
CACTTAGACCGGTCCTGGCT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MIPQPFIDDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGSAIGFLMEFSGLSYV
EAIRDLAQSVGMAVPEERGGLPPAARAERQAKTVALGDVMARASDHYRKQLRGAPGAVQYLKGRGLTGEIAGHFGLGYAP
DDWQSLESVFGAYRDDAIATPLIEAGLVIESEKTDADGRHRRYDRFRDRIMFPIRNTKGVVIGFGGRVLGQGEPKYLNSP
ETPLFSKGTELYGLFEARNAIREFGYVLVVEGYMDVVALAQLGFANAVATLGTACTPVHVQKLLRQVDTVIFSFDGDAAG
RRAARRALEACLPHVTDNKIVKFLFLPPEHDPDTFIREEGTEAFAAEVRNAMPLSRFLLQAVTEDLDLRQPEGRARAQYE
AKPLLSAMPPGGLRLQIVRGLAEMTGTTPADIEALCGLRSDPAQAGRMTQKRPRVARTAPTALEQKVLRLLMRYPALAAR
LDADTRTQLTAPELPQGEVLSGLLAECDTAGPEMHFGAFVERLGQTPYAEAFAGLRVAVLNDDIELEPATQEFDAAVQKM
LAEPLRRELEMLQRKMEGGHATDADKERLRWLVSEISRRRRLV

Sequences:

>Translated_603_residues
MIPQPFIDDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGSAIGFLMEFSGLSYV
EAIRDLAQSVGMAVPEERGGLPPAARAERQAKTVALGDVMARASDHYRKQLRGAPGAVQYLKGRGLTGEIAGHFGLGYAP
DDWQSLESVFGAYRDDAIATPLIEAGLVIESEKTDADGRHRRYDRFRDRIMFPIRNTKGVVIGFGGRVLGQGEPKYLNSP
ETPLFSKGTELYGLFEARNAIREFGYVLVVEGYMDVVALAQLGFANAVATLGTACTPVHVQKLLRQVDTVIFSFDGDAAG
RRAARRALEACLPHVTDNKIVKFLFLPPEHDPDTFIREEGTEAFAAEVRNAMPLSRFLLQAVTEDLDLRQPEGRARAQYE
AKPLLSAMPPGGLRLQIVRGLAEMTGTTPADIEALCGLRSDPAQAGRMTQKRPRVARTAPTALEQKVLRLLMRYPALAAR
LDADTRTQLTAPELPQGEVLSGLLAECDTAGPEMHFGAFVERLGQTPYAEAFAGLRVAVLNDDIELEPATQEFDAAVQKM
LAEPLRRELEMLQRKMEGGHATDADKERLRWLVSEISRRRRLV
>Mature_603_residues
MIPQPFIDDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGSAIGFLMEFSGLSYV
EAIRDLAQSVGMAVPEERGGLPPAARAERQAKTVALGDVMARASDHYRKQLRGAPGAVQYLKGRGLTGEIAGHFGLGYAP
DDWQSLESVFGAYRDDAIATPLIEAGLVIESEKTDADGRHRRYDRFRDRIMFPIRNTKGVVIGFGGRVLGQGEPKYLNSP
ETPLFSKGTELYGLFEARNAIREFGYVLVVEGYMDVVALAQLGFANAVATLGTACTPVHVQKLLRQVDTVIFSFDGDAAG
RRAARRALEACLPHVTDNKIVKFLFLPPEHDPDTFIREEGTEAFAAEVRNAMPLSRFLLQAVTEDLDLRQPEGRARAQYE
AKPLLSAMPPGGLRLQIVRGLAEMTGTTPADIEALCGLRSDPAQAGRMTQKRPRVARTAPTALEQKVLRLLMRYPALAAR
LDADTRTQLTAPELPQGEVLSGLLAECDTAGPEMHFGAFVERLGQTPYAEAFAGLRVAVLNDDIELEPATQEFDAAVQKM
LAEPLRRELEMLQRKMEGGHATDADKERLRWLVSEISRRRRLV

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=598, Percent_Identity=41.304347826087, Blast_Score=408, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 66307; Mature: 66307

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPQPFIDDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEECCHHHHHHHHC
CGAHGSAIGFLMEFSGLSYVEAIRDLAQSVGMAVPEERGGLPPAARAERQAKTVALGDVM
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHH
ARASDHYRKQLRGAPGAVQYLKGRGLTGEIAGHFGLGYAPDDWQSLESVFGAYRDDAIAT
HHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHH
PLIEAGLVIESEKTDADGRHRRYDRFRDRIMFPIRNTKGVVIGFGGRVLGQGEPKYLNSP
HHHHCCEEEECCCCCCCCHHHHHHHHHHHEEEEEECCCEEEEEECCEEECCCCCCCCCCC
ETPLFSKGTELYGLFEARNAIREFGYVLVVEGYMDVVALAQLGFANAVATLGTACTPVHV
CCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
QKLLRQVDTVIFSFDGDAAGRRAARRALEACLPHVTDNKIVKFLFLPPEHDPDTFIREEG
HHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHCC
TEAFAAEVRNAMPLSRFLLQAVTEDLDLRQPEGRARAQYEAKPLLSAMPPGGLRLQIVRG
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHH
LAEMTGTTPADIEALCGLRSDPAQAGRMTQKRPRVARTAPTALEQKVLRLLMRYPALAAR
HHHHCCCCCHHHHHHCCCCCCHHHHCCCHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHH
LDADTRTQLTAPELPQGEVLSGLLAECDTAGPEMHFGAFVERLGQTPYAEAFAGLRVAVL
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHCCCEEEEE
NDDIELEPATQEFDAAVQKMLAEPLRRELEMLQRKMEGGHATDADKERLRWLVSEISRRR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
RLV
CCC
>Mature Secondary Structure
MIPQPFIDDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEECCHHHHHHHHC
CGAHGSAIGFLMEFSGLSYVEAIRDLAQSVGMAVPEERGGLPPAARAERQAKTVALGDVM
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHH
ARASDHYRKQLRGAPGAVQYLKGRGLTGEIAGHFGLGYAPDDWQSLESVFGAYRDDAIAT
HHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHH
PLIEAGLVIESEKTDADGRHRRYDRFRDRIMFPIRNTKGVVIGFGGRVLGQGEPKYLNSP
HHHHCCEEEECCCCCCCCHHHHHHHHHHHEEEEEECCCEEEEEECCEEECCCCCCCCCCC
ETPLFSKGTELYGLFEARNAIREFGYVLVVEGYMDVVALAQLGFANAVATLGTACTPVHV
CCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
QKLLRQVDTVIFSFDGDAAGRRAARRALEACLPHVTDNKIVKFLFLPPEHDPDTFIREEG
HHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHCC
TEAFAAEVRNAMPLSRFLLQAVTEDLDLRQPEGRARAQYEAKPLLSAMPPGGLRLQIVRG
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHH
LAEMTGTTPADIEALCGLRSDPAQAGRMTQKRPRVARTAPTALEQKVLRLLMRYPALAAR
HHHHCCCCCHHHHHHCCCCCCHHHHCCCHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHH
LDADTRTQLTAPELPQGEVLSGLLAECDTAGPEMHFGAFVERLGQTPYAEAFAGLRVAVL
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHCCCEEEEE
NDDIELEPATQEFDAAVQKMLAEPLRRELEMLQRKMEGGHATDADKERLRWLVSEISRRR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
RLV
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]