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The map label for this gene is cafA [H]

Identifier: 17546916

GI number: 17546916

Start: 2378337

End: 2379806

Strand: Direct

Name: cafA [H]

Synonym: RSc2197

Alternate gene names: 17546916

Gene position: 2378337-2379806 (Clockwise)

Preceding gene: 17546915

Following gene: 17546917

Centisome position: 64.0

GC content: 66.05

Gene sequence:

>1470_bases
ATGTCCGAAGACATCCTCGTCAATATCACCCCGCAAGAAACGCGCGTAGCCATCGTCCAGCAGGCCGCCGTCCAGGAACT
CCACATCGAACGCACGCTGACGCGCGGGCTGGTCGGCAACATCTATCTCGGCAAGGTCGTGCGCGTGCTGCCGGGCATGC
AGTCGGCCTTCATCGACATCGGGCTGGAGCGCGCGGCCTTCCTGCACGTGGCCGACATCTGGCACCCGCGCGAAGCCAAG
GACGCCCCGGCCGCCCCGCACGTCGCCATCGAGAAGACGCTGTTCGAAGGCCAGGCGCTGACGGTGCAGGTCATCAAGGA
CCCGATCGGCACCAAGGGCGCGCGGCTGTCCACGCAGATCAGCATCGCCGGCCGCACGCTGGTCTACCTGCCGCAAGACC
CGCACATCGGCATCTCGCAGAAGATCGGCAACGAGACCGACCGCGAGGCGCTGCGCCAGCGCGTGACCCGCATGGTGCCG
GCCGACGAGCGCGGCGGCTTCATCGTGCGCACCATCGCCGAGGAAGCCACCGACGAAGAACTGGCCAACGACGTCGCCTA
CCTGCGCAAGATCTGGGCGACCATCCGCCACAACGCCACTACCCTGCCCGCGCCGTCGATCCTGTACCAGGACCTGAACC
TCGCCCAGCGCGTGCTGCGCGATTTCGTGACCGACGACACGCGCAGCATCCAGATCGATTCGCGCGAGAACTACCAGAAG
CTGATCGAGTTCGCGCAGGAATACACGCCCGCCGTGGTGGAGCGCCTGACCCACTACACCGGCGAGCGGCCGATCTTCGA
TCTGTACAACATCGAGGCGGAGGTCGAGCGCGCGCTGTCGCGCCGGGTCGACCTGAAGTCCGGCGGCTACCTGATGATCG
ACCAGACCGAGGCGATGACGACCATCGACGTCAACACCGGCGGCTACGTGGGCGCGCGCAACTTCGACGACACCATCTTC
AAGACCAACCTGGAAGCGGCGCACACCATCGCGCGCCAGCTGCGGCTGCGCAACCTGGGCGGCATCATCATCATCGACTT
CATCGACATGGAGTCCGGCGAGCACCGCGACGCCGTGCTGGCCGAGCTGCGCCGCGCGCTGGCGCGCGACCGCACGCGCA
TCACGGTCAACAGCTTCTCGCAGCTGGGCCTGGTGGAGATGACCCGCAAGCGCACGCGCGAATCGCTCGCGCACGTGCTG
TGCGAGCAGTGTCCGGTGTGCCAGGGCAAGGGCCAGGTGAAGACGCCGCGCACGGTGTGCTACGACATCCTGCGCGAGAT
CATGCGCGAATCCCGCCAGTTCAACCCGCGCGAGTTCCGCATCCTCGCCTCGCAGTCGGTGATCGATCTGTTCCTGGAAG
AGGAAAGCCAGCATCTGGCGATGCTGGGCGACTTCATCGGCAAGCCGATCTCGCTGCAGGTGGAATCGGCGGCGGCTAGC
CAGGAGCAGTACGACATCATTTTGATGTGA

Upstream 100 bases:

>100_bases
CGCCGGACCGCGTGCGAGGGCCGGTTTCAAGACACGCTTCAGGCGAGCATGGCCAGCCCTGGAGGCGGGTGCTGAAACCC
GCTCTCTTGGTATTCCGCCC

Downstream 100 bases:

>100_bases
TTCAAGCGCACCTTTTCAGGACGATGCCCGAAGTGGCCCCTGCCCCGCATGGCATCGCCTAGAATTTTTCCATCACCCGA
ACACCACGCCGCGGCGCGCC

Product: ribonuclease G

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MSEDILVNITPQETRVAIVQQAAVQELHIERTLTRGLVGNIYLGKVVRVLPGMQSAFIDIGLERAAFLHVADIWHPREAK
DAPAAPHVAIEKTLFEGQALTVQVIKDPIGTKGARLSTQISIAGRTLVYLPQDPHIGISQKIGNETDREALRQRVTRMVP
ADERGGFIVRTIAEEATDEELANDVAYLRKIWATIRHNATTLPAPSILYQDLNLAQRVLRDFVTDDTRSIQIDSRENYQK
LIEFAQEYTPAVVERLTHYTGERPIFDLYNIEAEVERALSRRVDLKSGGYLMIDQTEAMTTIDVNTGGYVGARNFDDTIF
KTNLEAAHTIARQLRLRNLGGIIIIDFIDMESGEHRDAVLAELRRALARDRTRITVNSFSQLGLVEMTRKRTRESLAHVL
CEQCPVCQGKGQVKTPRTVCYDILREIMRESRQFNPREFRILASQSVIDLFLEEESQHLAMLGDFIGKPISLQVESAAAS
QEQYDIILM

Sequences:

>Translated_489_residues
MSEDILVNITPQETRVAIVQQAAVQELHIERTLTRGLVGNIYLGKVVRVLPGMQSAFIDIGLERAAFLHVADIWHPREAK
DAPAAPHVAIEKTLFEGQALTVQVIKDPIGTKGARLSTQISIAGRTLVYLPQDPHIGISQKIGNETDREALRQRVTRMVP
ADERGGFIVRTIAEEATDEELANDVAYLRKIWATIRHNATTLPAPSILYQDLNLAQRVLRDFVTDDTRSIQIDSRENYQK
LIEFAQEYTPAVVERLTHYTGERPIFDLYNIEAEVERALSRRVDLKSGGYLMIDQTEAMTTIDVNTGGYVGARNFDDTIF
KTNLEAAHTIARQLRLRNLGGIIIIDFIDMESGEHRDAVLAELRRALARDRTRITVNSFSQLGLVEMTRKRTRESLAHVL
CEQCPVCQGKGQVKTPRTVCYDILREIMRESRQFNPREFRILASQSVIDLFLEEESQHLAMLGDFIGKPISLQVESAAAS
QEQYDIILM
>Mature_488_residues
SEDILVNITPQETRVAIVQQAAVQELHIERTLTRGLVGNIYLGKVVRVLPGMQSAFIDIGLERAAFLHVADIWHPREAKD
APAAPHVAIEKTLFEGQALTVQVIKDPIGTKGARLSTQISIAGRTLVYLPQDPHIGISQKIGNETDREALRQRVTRMVPA
DERGGFIVRTIAEEATDEELANDVAYLRKIWATIRHNATTLPAPSILYQDLNLAQRVLRDFVTDDTRSIQIDSRENYQKL
IEFAQEYTPAVVERLTHYTGERPIFDLYNIEAEVERALSRRVDLKSGGYLMIDQTEAMTTIDVNTGGYVGARNFDDTIFK
TNLEAAHTIARQLRLRNLGGIIIIDFIDMESGEHRDAVLAELRRALARDRTRITVNSFSQLGLVEMTRKRTRESLAHVLC
EQCPVCQGKGQVKTPRTVCYDILREIMRESRQFNPREFRILASQSVIDLFLEEESQHLAMLGDFIGKPISLQVESAAASQ
EQYDIILM

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=492, Percent_Identity=52.6422764227642, Blast_Score=507, Evalue=1e-145,
Organism=Escherichia coli, GI1787325, Length=459, Percent_Identity=37.9084967320261, Blast_Score=280, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 55155; Mature: 55024

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDILVNITPQETRVAIVQQAAVQELHIERTLTRGLVGNIYLGKVVRVLPGMQSAFIDI
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHH
GLERAAFLHVADIWHPREAKDAPAAPHVAIEKTLFEGQALTVQVIKDPIGTKGARLSTQI
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEE
SIAGRTLVYLPQDPHIGISQKIGNETDREALRQRVTRMVPADERGGFIVRTIAEEATDEE
EECCEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHCCHHH
LANDVAYLRKIWATIRHNATTLPAPSILYQDLNLAQRVLRDFVTDDTRSIQIDSRENYQK
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHH
LIEFAQEYTPAVVERLTHYTGERPIFDLYNIEAEVERALSRRVDLKSGGYLMIDQTEAMT
HHHHHHHHCHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCEEEEECCCCEE
TIDVNTGGYVGARNFDDTIFKTNLEAAHTIARQLRLRNLGGIIIIDFIDMESGEHRDAVL
EEEECCCCEECCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHH
AELRRALARDRTRITVNSFSQLGLVEMTRKRTRESLAHVLCEQCPVCQGKGQVKTPRTVC
HHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
YDILREIMRESRQFNPREFRILASQSVIDLFLEEESQHLAMLGDFIGKPISLQVESAAAS
HHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEHHHHCC
QEQYDIILM
CCCCEEEEC
>Mature Secondary Structure 
SEDILVNITPQETRVAIVQQAAVQELHIERTLTRGLVGNIYLGKVVRVLPGMQSAFIDI
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHH
GLERAAFLHVADIWHPREAKDAPAAPHVAIEKTLFEGQALTVQVIKDPIGTKGARLSTQI
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEE
SIAGRTLVYLPQDPHIGISQKIGNETDREALRQRVTRMVPADERGGFIVRTIAEEATDEE
EECCEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHCCHHH
LANDVAYLRKIWATIRHNATTLPAPSILYQDLNLAQRVLRDFVTDDTRSIQIDSRENYQK
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHH
LIEFAQEYTPAVVERLTHYTGERPIFDLYNIEAEVERALSRRVDLKSGGYLMIDQTEAMT
HHHHHHHHCHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCEEEEECCCCEE
TIDVNTGGYVGARNFDDTIFKTNLEAAHTIARQLRLRNLGGIIIIDFIDMESGEHRDAVL
EEEECCCCEECCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHH
AELRRALARDRTRITVNSFSQLGLVEMTRKRTRESLAHVLCEQCPVCQGKGQVKTPRTVC
HHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
YDILREIMRESRQFNPREFRILASQSVIDLFLEEESQHLAMLGDFIGKPISLQVESAAAS
HHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEHHHHCC
QEQYDIILM
CCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]