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The map label for this gene is rlmH

Identifier: 17546914

GI number: 17546914

Start: 2377111

End: 2377581

Strand: Direct

Name: rlmH

Synonym: RSc2195

Alternate gene names: 17546914

Gene position: 2377111-2377581 (Clockwise)

Preceding gene: 17546913

Following gene: 17546915

Centisome position: 63.96

GC content: 69.64

Gene sequence:

>471_bases
ATGCAGTTGCTGATCGTCGCAGTCGGGCACAAGATGCCGCGCTGGATCGAAGACGGCTTCGCCGAATACGCCAAGCGCAT
GCCGCCCGAACTGCGCATCGAGCTGCGCGAAATCAAGCCGGAGCAGCGCTCCGGCAGCCGCACCGCCGCCACCGTCATGC
AGCTCGAAGCCGCGCGCATCGAAGCCGCGCTGCCCAAGGGCTGCCGCATCGTCGCGCTGGACGAGCGCGGCAAGGACCTG
ACCACGGCCGCACTGGCCGACGCCCTCACCGGCTGGCAGCGCGAGGGCGGTGACGTCGCCCTCATCATCGGCGGCGCGGA
TGGGCTGGATCCGGCGCTCAAGGCGCGCGCCCACATGCTGATGCGCCTGTCGAGCCTGACGCTGCCGCACGGCATGGTGC
GCGTGCTGCTGGCCGAGCAGCTCTACCGCGCATGGAGCATCACGCAGAACCACCCCTACCATCGCGTGTGA

Upstream 100 bases:

>100_bases
TTCCGGCACGAGCAAGCCCGCCACGAAGACCGCCGCAAAAACGGCTGCAAAGCGCGCCCCGGCCAAGCGCGCATCGTAAT
CCCGCGCGGGCAGCCTCGCC

Downstream 100 bases:

>100_bases
CGATGGAACCGACCGCCGCGCCGCATCCGCACCTGTACCTCGCCTCGCAAAGCCCGCGCCGGCAGGAGCTGCTGCGCCAG
ATCGGCGTGCGCTTCGAACT

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MQLLIVAVGHKMPRWIEDGFAEYAKRMPPELRIELREIKPEQRSGSRTAATVMQLEAARIEAALPKGCRIVALDERGKDL
TTAALADALTGWQREGGDVALIIGGADGLDPALKARAHMLMRLSSLTLPHGMVRVLLAEQLYRAWSITQNHPYHRV

Sequences:

>Translated_156_residues
MQLLIVAVGHKMPRWIEDGFAEYAKRMPPELRIELREIKPEQRSGSRTAATVMQLEAARIEAALPKGCRIVALDERGKDL
TTAALADALTGWQREGGDVALIIGGADGLDPALKARAHMLMRLSSLTLPHGMVRVLLAEQLYRAWSITQNHPYHRV
>Mature_156_residues
MQLLIVAVGHKMPRWIEDGFAEYAKRMPPELRIELREIKPEQRSGSRTAATVMQLEAARIEAALPKGCRIVALDERGKDL
TTAALADALTGWQREGGDVALIIGGADGLDPALKARAHMLMRLSSLTLPHGMVRVLLAEQLYRAWSITQNHPYHRV

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=155, Percent_Identity=50.3225806451613, Blast_Score=144, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_RALSO (Q8XXC0)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520316.1
- ProteinModelPortal:   Q8XXC0
- SMR:   Q8XXC0
- GeneID:   1221039
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2195
- NMPDR:   fig|267608.1.peg.2195
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- BioCyc:   RSOL267608:RSC2195-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17314; Mature: 17314

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLLIVAVGHKMPRWIEDGFAEYAKRMPPELRIELREIKPEQRSGSRTAATVMQLEAARI
CEEEEEECCCCCCHHHHHHHHHHHHHCCHHHEEHHHHCCCCCCCCCHHHHHHHHHHHHHH
EAALPKGCRIVALDERGKDLTTAALADALTGWQREGGDVALIIGGADGLDPALKARAHML
HHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHH
MRLSSLTLPHGMVRVLLAEQLYRAWSITQNHPYHRV
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQLLIVAVGHKMPRWIEDGFAEYAKRMPPELRIELREIKPEQRSGSRTAATVMQLEAARI
CEEEEEECCCCCCHHHHHHHHHHHHHCCHHHEEHHHHCCCCCCCCCHHHHHHHHHHHHHH
EAALPKGCRIVALDERGKDLTTAALADALTGWQREGGDVALIIGGADGLDPALKARAHML
HHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHH
MRLSSLTLPHGMVRVLLAEQLYRAWSITQNHPYHRV
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852