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The map label for this gene is spoT [H]

Identifier: 17546872

GI number: 17546872

Start: 2335348

End: 2337555

Strand: Reverse

Name: spoT [H]

Synonym: RSc2153

Alternate gene names: 17546872

Gene position: 2337555-2335348 (Counterclockwise)

Preceding gene: 17546873

Following gene: 17546871

Centisome position: 62.9

GC content: 67.03

Gene sequence:

>2208_bases
TTGTTCGGGCCGACCTCGCAGCCGGCCGCGCCGCCGCGCCAGCAGGTGGTCTCCATCGCGGGGCTGACGGAGAAGCTGTC
GTACCTGAAGCCGGCCGACATCAAGCTGGTGAAGGAAGCCTTCCACTTCTCCGACGAGGCGCACCTCGGCCAGTACCGCC
AGAGCGGCGAGCCGTACATCACGCACCCGGTGGCGGTGGCGGAAATCTGCGCGGGCTGGAAGCTGGACGTGCAATCGATC
ATGGCCGCGCTGCTGCACGACGTGATCGAGGACCAGGGCGTCACCAAGAGCGAACTGGCCGAGAAGTTCGGCCCCAAGGT
CGCGGAGCTGGTCGACGGCCTCACCAAGCTGGACAAGCTCGAATTCCAGAGCCGCGAGCAGGCGCAGGCGGAGAGCTTCC
GCAAGATGCTGCTGGCGATGGCGCGCGACGTGCGCGTGATCCTGGTCAAGCTGGCCGACCGCACGCACAACATGCGCACG
CTCGACCACGTGCCGCCCGAAAAGCGCCGCCGCATCGCCGGCGAGACGATGGAGATCTACGCGCCGATCGCGCACCGGCT
CGGGCTCAATACCACGTACCGCGAACTGCAGGAGTTGAGCTTTCGCATCGGCTCGCCGTTCCGCTATGCCACGCTGGAGA
AGGCCGTCAAGGCCGCGCGCGGCAACCGGCGCGAGGTGGTCAGCCGCATCCTGGAGGCCGCGCAGCGCGCCCTGGGCGAC
GCGGGCGTTTCGGCCGAGCTGACCGGCCGCGAGAAGACGCTCTACAGCATCTACCGCAAGATGCACGACAAGCAGCTGTC
GTTCTCGCAGGTGCTGGATGTGTACGGCTTCCGCGTGGTGGTCGAGACGCAGATGCACTGCTACATGGCGGTGGGCGCGC
TGCACGGTCTGTACAAGCCGATGCCCGGCAAGTTCAAGGACTACATCGCCATCCCCAAGATCAACGGCTACCAGTCCGTG
CACACCACGCTGGTGGGGCCGTTCGGCACGCCGGTCGAATTCCAGATCCGCACGCGCGAGATGAACCAGATCGCCGAGGC
TGGAGTGGCGGCGCACTGGATGTACAAGCAGCACCACGACGAGCCGGATCGCGCGCAGCAGCAGGCGCACCAGTGGCTGC
AGTCGCTGCTCGATATCCAGAGCCAGAGCGGCGATTCGCAGGAGTTCCTGGAGCACGTCAAGATCGACCTGTTCCCGGAT
GCCGTCTACGTGTTCACGCCCAAGGGCGAGATCCGCGCGCTGCCGCGCGGCGCCACGGCGTTGGACTTCGCCTATGCCGT
GCACAGCGACCTGGGCAACCAGTGCGTGGCGGTCAAGATCAACAATGCGCTGCTGCCGCTGCGTACCGAGCTCAAGAACG
GCGATATCGTCGAGGTGGTGACGGCGCCGTACTCCAAGCCGAACCCGGCGTGGCTCACGTTCGTGCGTACCGGCAAGGCG
CGCGCGGCGATCCGCCATTTCCTGAAGACCGCCAAGCTGGACGAAGCCATTCAGCTGGGCGAGCGGCTGCTGGAGCAGGC
GCTGCGCCAGCTGGGCATCGACATGAAAGCCGTGCCCGCGCAGGTGTGGGAGCGGATCGTGCAGTGGACCGGCAACAAGG
CGCGCGAAGACATCTTCGCCGACCTGGCGCTGGGCCGGCGCGTGGCCGCCGTGGTGGCGCGGCGCATCGAGATCGGGTTG
CAGGAGGGCGGGCACGAGGGCGATGAAGCGCTGATCGCCGCCGTCCACGCCTTCGCCGGCGAAGAGGCGCCCGCCGTGAC
GGTCAGCGGCGACGAAGGCATGGCCATGGTGTTCTCGCCGTGCTGCCGGCCGATTCCCGGCGACCCGATCGTCGGCTACA
TCGGCAAGGGCGAAGGGCTGCAGATCCACGTGGAAGAATGTCGCGTGGCCAAGCGCCTGCACGGCAAGGATCCGGAGCAC
TGGATCGACGTCATGTGGGCGGAGCACACCACGCGCGCCTTCGATGTGTCGATCAAGGTGCTGGTGCGCAATACCAAGGG
CATTCTCGCGCGGGTGGCCGCGGACCTGACCTCGGCCGATGCCAACGTCGCGCATGTGTCGATGGAGCAGGAGGGTGGTG
GCCAGGAAGCCACGTACATGACCTTCCTGATCCAGGTGCATGACCGCGTGCACCTGGCCGACGTGATGCGTGCGCTGCGA
CGCAACCTCGATGTGATCCGCATCGCGCGGGACCGGGGTGGAGAGTAG

Upstream 100 bases:

>100_bases
GCAGGGCGCGGATGCATCCGCGCCGGCTGCCGCCAGGGCCGAGCCGCCCGCGGGTGAGACCCTCTTTATCGACGCGGTGC
TCGAGCAGACGTACCGACAT

Downstream 100 bases:

>100_bases
CGCGGAGGCATTGCCGCCGACGGAGATGAGGAAAGGCGAAGCTGCGGCTTCGCCTTTTTTGTTGGTGCGCGGTGCGCATG
GCGCCGGCTTGCGGTGCGAG

Product: bifunctional (P)ppGpp synthetase II/guanosine-3',5'-bisdiphosphate 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 735; Mature: 735

Protein sequence:

>735_residues
MFGPTSQPAAPPRQQVVSIAGLTEKLSYLKPADIKLVKEAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDVQSI
MAALLHDVIEDQGVTKSELAEKFGPKVAELVDGLTKLDKLEFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRT
LDHVPPEKRRRIAGETMEIYAPIAHRLGLNTTYRELQELSFRIGSPFRYATLEKAVKAARGNRREVVSRILEAAQRALGD
AGVSAELTGREKTLYSIYRKMHDKQLSFSQVLDVYGFRVVVETQMHCYMAVGALHGLYKPMPGKFKDYIAIPKINGYQSV
HTTLVGPFGTPVEFQIRTREMNQIAEAGVAAHWMYKQHHDEPDRAQQQAHQWLQSLLDIQSQSGDSQEFLEHVKIDLFPD
AVYVFTPKGEIRALPRGATALDFAYAVHSDLGNQCVAVKINNALLPLRTELKNGDIVEVVTAPYSKPNPAWLTFVRTGKA
RAAIRHFLKTAKLDEAIQLGERLLEQALRQLGIDMKAVPAQVWERIVQWTGNKAREDIFADLALGRRVAAVVARRIEIGL
QEGGHEGDEALIAAVHAFAGEEAPAVTVSGDEGMAMVFSPCCRPIPGDPIVGYIGKGEGLQIHVEECRVAKRLHGKDPEH
WIDVMWAEHTTRAFDVSIKVLVRNTKGILARVAADLTSADANVAHVSMEQEGGGQEATYMTFLIQVHDRVHLADVMRALR
RNLDVIRIARDRGGE

Sequences:

>Translated_735_residues
MFGPTSQPAAPPRQQVVSIAGLTEKLSYLKPADIKLVKEAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDVQSI
MAALLHDVIEDQGVTKSELAEKFGPKVAELVDGLTKLDKLEFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRT
LDHVPPEKRRRIAGETMEIYAPIAHRLGLNTTYRELQELSFRIGSPFRYATLEKAVKAARGNRREVVSRILEAAQRALGD
AGVSAELTGREKTLYSIYRKMHDKQLSFSQVLDVYGFRVVVETQMHCYMAVGALHGLYKPMPGKFKDYIAIPKINGYQSV
HTTLVGPFGTPVEFQIRTREMNQIAEAGVAAHWMYKQHHDEPDRAQQQAHQWLQSLLDIQSQSGDSQEFLEHVKIDLFPD
AVYVFTPKGEIRALPRGATALDFAYAVHSDLGNQCVAVKINNALLPLRTELKNGDIVEVVTAPYSKPNPAWLTFVRTGKA
RAAIRHFLKTAKLDEAIQLGERLLEQALRQLGIDMKAVPAQVWERIVQWTGNKAREDIFADLALGRRVAAVVARRIEIGL
QEGGHEGDEALIAAVHAFAGEEAPAVTVSGDEGMAMVFSPCCRPIPGDPIVGYIGKGEGLQIHVEECRVAKRLHGKDPEH
WIDVMWAEHTTRAFDVSIKVLVRNTKGILARVAADLTSADANVAHVSMEQEGGGQEATYMTFLIQVHDRVHLADVMRALR
RNLDVIRIARDRGGE
>Mature_735_residues
MFGPTSQPAAPPRQQVVSIAGLTEKLSYLKPADIKLVKEAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDVQSI
MAALLHDVIEDQGVTKSELAEKFGPKVAELVDGLTKLDKLEFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRT
LDHVPPEKRRRIAGETMEIYAPIAHRLGLNTTYRELQELSFRIGSPFRYATLEKAVKAARGNRREVVSRILEAAQRALGD
AGVSAELTGREKTLYSIYRKMHDKQLSFSQVLDVYGFRVVVETQMHCYMAVGALHGLYKPMPGKFKDYIAIPKINGYQSV
HTTLVGPFGTPVEFQIRTREMNQIAEAGVAAHWMYKQHHDEPDRAQQQAHQWLQSLLDIQSQSGDSQEFLEHVKIDLFPD
AVYVFTPKGEIRALPRGATALDFAYAVHSDLGNQCVAVKINNALLPLRTELKNGDIVEVVTAPYSKPNPAWLTFVRTGKA
RAAIRHFLKTAKLDEAIQLGERLLEQALRQLGIDMKAVPAQVWERIVQWTGNKAREDIFADLALGRRVAAVVARRIEIGL
QEGGHEGDEALIAAVHAFAGEEAPAVTVSGDEGMAMVFSPCCRPIPGDPIVGYIGKGEGLQIHVEECRVAKRLHGKDPEH
WIDVMWAEHTTRAFDVSIKVLVRNTKGILARVAADLTSADANVAHVSMEQEGGGQEATYMTFLIQVHDRVHLADVMRALR
RNLDVIRIARDRGGE

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=134, Percent_Identity=37.3134328358209, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1790082, Length=711, Percent_Identity=47.1167369901547, Blast_Score=672, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=698, Percent_Identity=32.378223495702, Blast_Score=377, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 81863; Mature: 81863

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGPTSQPAAPPRQQVVSIAGLTEKLSYLKPADIKLVKEAFHFSDEAHLGQYRQSGEPYI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEE
THPVAVAEICAGWKLDVQSIMAALLHDVIEDQGVTKSELAEKFGPKVAELVDGLTKLDKL
ECHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHH
EFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRTLDHVPPEKRRRIAGETMEIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHHH
APIAHRLGLNTTYRELQELSFRIGSPFRYATLEKAVKAARGNRREVVSRILEAAQRALGD
HHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
AGVSAELTGREKTLYSIYRKMHDKQLSFSQVLDVYGFRVVVETQMHCYMAVGALHGLYKP
CCCCEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCC
MPGKFKDYIAIPKINGYQSVHTTLVGPFGTPVEFQIRTREMNQIAEAGVAAHWMYKQHHD
CCCCCCCEEECCCCCCCHHHHHHEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCC
EPDRAQQQAHQWLQSLLDIQSQSGDSQEFLEHVKIDLFPDAVYVFTPKGEIRALPRGATA
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCEEEEECCCCCEEECCCCCHH
LDFAYAVHSDLGNQCVAVKINNALLPLRTELKNGDIVEVVTAPYSKPNPAWLTFVRTGKA
HHHHHHHHHCCCCCEEEEEECCCEECHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCCH
RAAIRHFLKTAKLDEAIQLGERLLEQALRQLGIDMKAVPAQVWERIVQWTGNKAREDIFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
DLALGRRVAAVVARRIEIGLQEGGHEGDEALIAAVHAFAGEEAPAVTVSGDEGMAMVFSP
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHH
CCRPIPGDPIVGYIGKGEGLQIHVEECRVAKRLHGKDPEHWIDVMWAEHTTRAFDVSIKV
CCCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEEEE
LVRNTKGILARVAADLTSADANVAHVSMEQEGGGQEATYMTFLIQVHDRVHLADVMRALR
EEECHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
RNLDVIRIARDRGGE
CCCCEEEEECCCCCC
>Mature Secondary Structure
MFGPTSQPAAPPRQQVVSIAGLTEKLSYLKPADIKLVKEAFHFSDEAHLGQYRQSGEPYI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEE
THPVAVAEICAGWKLDVQSIMAALLHDVIEDQGVTKSELAEKFGPKVAELVDGLTKLDKL
ECHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHH
EFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRTLDHVPPEKRRRIAGETMEIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHHH
APIAHRLGLNTTYRELQELSFRIGSPFRYATLEKAVKAARGNRREVVSRILEAAQRALGD
HHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
AGVSAELTGREKTLYSIYRKMHDKQLSFSQVLDVYGFRVVVETQMHCYMAVGALHGLYKP
CCCCEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCC
MPGKFKDYIAIPKINGYQSVHTTLVGPFGTPVEFQIRTREMNQIAEAGVAAHWMYKQHHD
CCCCCCCEEECCCCCCCHHHHHHEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCC
EPDRAQQQAHQWLQSLLDIQSQSGDSQEFLEHVKIDLFPDAVYVFTPKGEIRALPRGATA
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCEEEEECCCCCEEECCCCCHH
LDFAYAVHSDLGNQCVAVKINNALLPLRTELKNGDIVEVVTAPYSKPNPAWLTFVRTGKA
HHHHHHHHHCCCCCEEEEEECCCEECHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCCH
RAAIRHFLKTAKLDEAIQLGERLLEQALRQLGIDMKAVPAQVWERIVQWTGNKAREDIFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
DLALGRRVAAVVARRIEIGLQEGGHEGDEALIAAVHAFAGEEAPAVTVSGDEGMAMVFSP
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHH
CCRPIPGDPIVGYIGKGEGLQIHVEECRVAKRLHGKDPEHWIDVMWAEHTTRAFDVSIKV
CCCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEEEE
LVRNTKGILARVAADLTSADANVAHVSMEQEGGGQEATYMTFLIQVHDRVHLADVMRALR
EEECHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
RNLDVIRIARDRGGE
CCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]