| Definition | Ralstonia solanacearum GMI1000, complete genome. |
|---|---|
| Accession | NC_003295 |
| Length | 3,716,413 |
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The map label for this gene is polS [H]
Identifier: 17546866
GI number: 17546866
Start: 2330228
End: 2330998
Strand: Reverse
Name: polS [H]
Synonym: RSc2147
Alternate gene names: 17546866
Gene position: 2330998-2330228 (Counterclockwise)
Preceding gene: 17546867
Following gene: 17546865
Centisome position: 62.72
GC content: 65.89
Gene sequence:
>771_bases TTGCAGGACAAGGTCGCGATCCTGACAGGGGCAGCCAGCGGCATCGGCGAAGCGGTCGCGCAACGCTATCTGGAAGCGGG CGCACGCTGCGTGCTCGTCGATTTGAAACCCGCGGGCGGCACGCTCGCGCAACTCATCGAAACGCATCCCGACCGTGCGT TCGCGCTGTCCGCCGACGTCACGAAACGTGACGATATCGAGCGCATCGTCTCGGCTGCGGTCGAGCGTTTCGGCGGCATC GACATCCTGTTCAACAACGCGGCCGCGTTCGACATGCGTCCGTTGCTCGACGAAGCCTGGGAGGTGTTCGACCGGCTGTT CGCGGTCAACGTGAAGGGCATGTTCTTTCTGATGCAGGCGGTTGCGCAGCGGATGGCGGCGCAGGGACGCGGCGGCAAGA TCATCAACATGGCTTCGCAGGCCGGCCGGCGCGGCGAGGCGCTGGTGTCGCACTATTGCGCGACGAAGGCGGCGGTCATC AGCTATACGCAGTCGGCTGCACTGGCGCTCGCGCCGTACAAGATCAACGTGAACGGGATCGCGCCGGGCGTCGTCGACAC GCCGATGTGGGAGCAGGTCGACGCGCTGTTCGCGCGCTATGAGAATCGCCCACTCGGTGAGAAGAAGCGTCTGGTCGGTG AGGCGGTACCGCTCGGCCGGATGGGGGTGCCGGCTGACCTGACGGGCGCCGCGCTGTTTCTCGCGTCGACCGACGCCGAC TACATCACCGCTCAGACGCTGAACGTCGACGGCGGCAACTGGATGAGCTGA
Upstream 100 bases:
>100_bases ATCATTCGTTCATCGATAGAGCAATTGTTCTTGTAAGGGGCATTCGCTCGCATCTCGATACTTCATAAAACAGACGTTGA TTCGGAGACAGCCAGTGAGA
Downstream 100 bases:
>100_bases GGCCATGGCCGCGATCGTCGTAGCGGGCGAGTTGCTCGCCGAATTCGTTGCCGCCGAGCGGGGGCAGATGCTCGCCGAGC CCGGTGCATTCATCGGACCG
Product: sorbitol dehydrogenase
Products: NA
Alternate protein names: L-iditol 2-dehydrogenase; Polyol dehydrogenase [H]
Number of amino acids: Translated: 256; Mature: 256
Protein sequence:
>256_residues MQDKVAILTGAASGIGEAVAQRYLEAGARCVLVDLKPAGGTLAQLIETHPDRAFALSADVTKRDDIERIVSAAVERFGGI DILFNNAAAFDMRPLLDEAWEVFDRLFAVNVKGMFFLMQAVAQRMAAQGRGGKIINMASQAGRRGEALVSHYCATKAAVI SYTQSAALALAPYKINVNGIAPGVVDTPMWEQVDALFARYENRPLGEKKRLVGEAVPLGRMGVPADLTGAALFLASTDAD YITAQTLNVDGGNWMS
Sequences:
>Translated_256_residues MQDKVAILTGAASGIGEAVAQRYLEAGARCVLVDLKPAGGTLAQLIETHPDRAFALSADVTKRDDIERIVSAAVERFGGI DILFNNAAAFDMRPLLDEAWEVFDRLFAVNVKGMFFLMQAVAQRMAAQGRGGKIINMASQAGRRGEALVSHYCATKAAVI SYTQSAALALAPYKINVNGIAPGVVDTPMWEQVDALFARYENRPLGEKKRLVGEAVPLGRMGVPADLTGAALFLASTDAD YITAQTLNVDGGNWMS >Mature_256_residues MQDKVAILTGAASGIGEAVAQRYLEAGARCVLVDLKPAGGTLAQLIETHPDRAFALSADVTKRDDIERIVSAAVERFGGI DILFNNAAAFDMRPLLDEAWEVFDRLFAVNVKGMFFLMQAVAQRMAAQGRGGKIINMASQAGRRGEALVSHYCATKAAVI SYTQSAALALAPYKINVNGIAPGVVDTPMWEQVDALFARYENRPLGEKKRLVGEAVPLGRMGVPADLTGAALFLASTDAD YITAQTLNVDGGNWMS
Specific function: Catalyzes the oxidation of D-glucitol (sorbitol) to D- fructose, galactitol to D-tagatose and of L-iditol [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI32483357, Length=259, Percent_Identity=33.2046332046332, Blast_Score=119, Evalue=2e-27, Organism=Homo sapiens, GI59889578, Length=235, Percent_Identity=35.3191489361702, Blast_Score=119, Evalue=3e-27, Organism=Homo sapiens, GI7705925, Length=248, Percent_Identity=33.8709677419355, Blast_Score=115, Evalue=4e-26, Organism=Homo sapiens, GI15277342, Length=267, Percent_Identity=32.5842696629214, Blast_Score=113, Evalue=2e-25, Organism=Homo sapiens, GI19923817, Length=260, Percent_Identity=29.2307692307692, Blast_Score=108, Evalue=5e-24, Organism=Homo sapiens, GI126723750, Length=262, Percent_Identity=32.0610687022901, Blast_Score=105, Evalue=6e-23, Organism=Homo sapiens, GI10190704, Length=261, Percent_Identity=31.0344827586207, Blast_Score=103, Evalue=1e-22, Organism=Homo sapiens, GI4758504, Length=263, Percent_Identity=30.7984790874525, Blast_Score=99, Evalue=5e-21, Organism=Homo sapiens, GI5031737, Length=266, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=4e-20, Organism=Homo sapiens, GI31542939, Length=197, Percent_Identity=32.994923857868, Blast_Score=93, Evalue=3e-19, Organism=Homo sapiens, GI40254992, Length=252, Percent_Identity=29.7619047619048, Blast_Score=92, Evalue=5e-19, Organism=Homo sapiens, GI33667109, Length=258, Percent_Identity=29.4573643410853, Blast_Score=86, Evalue=3e-17, Organism=Homo sapiens, GI66933014, Length=253, Percent_Identity=32.8063241106719, Blast_Score=86, Evalue=3e-17, Organism=Homo sapiens, GI83715985, Length=263, Percent_Identity=28.8973384030418, Blast_Score=84, Evalue=1e-16, Organism=Homo sapiens, GI126723191, Length=184, Percent_Identity=32.0652173913043, Blast_Score=79, Evalue=5e-15, Organism=Homo sapiens, GI224922801, Length=168, Percent_Identity=32.7380952380952, Blast_Score=78, Evalue=8e-15, Organism=Homo sapiens, GI40807363, Length=193, Percent_Identity=26.4248704663212, Blast_Score=74, Evalue=2e-13, Organism=Homo sapiens, GI4503817, Length=220, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=3e-13, Organism=Homo sapiens, GI4504505, Length=189, Percent_Identity=31.2169312169312, Blast_Score=72, Evalue=3e-13, Organism=Homo sapiens, GI109715829, Length=248, Percent_Identity=29.0322580645161, Blast_Score=72, Evalue=6e-13, Organism=Homo sapiens, GI20149619, Length=200, Percent_Identity=28, Blast_Score=71, Evalue=8e-13, Organism=Homo sapiens, GI4503301, Length=232, Percent_Identity=25.4310344827586, Blast_Score=70, Evalue=3e-12, Organism=Homo sapiens, GI7706318, Length=193, Percent_Identity=29.0155440414508, Blast_Score=67, Evalue=2e-11, Organism=Escherichia coli, GI1789208, Length=256, Percent_Identity=35.546875, Blast_Score=147, Evalue=9e-37, Organism=Escherichia coli, GI87082160, Length=252, Percent_Identity=34.1269841269841, Blast_Score=123, Evalue=1e-29, Organism=Escherichia coli, GI87082100, Length=258, Percent_Identity=34.1085271317829, Blast_Score=119, Evalue=2e-28, Organism=Escherichia coli, GI1788459, Length=252, Percent_Identity=32.9365079365079, Blast_Score=116, Evalue=2e-27, Organism=Escherichia coli, GI1787905, Length=250, Percent_Identity=34, Blast_Score=112, Evalue=2e-26, Organism=Escherichia coli, GI1787335, Length=256, Percent_Identity=32.421875, Blast_Score=111, Evalue=5e-26, Organism=Escherichia coli, GI1790717, Length=252, Percent_Identity=30.952380952381, Blast_Score=110, Evalue=7e-26, Organism=Escherichia coli, GI1786812, Length=254, Percent_Identity=34.6456692913386, Blast_Score=97, Evalue=2e-21, Organism=Escherichia coli, GI1789378, Length=260, Percent_Identity=30, Blast_Score=96, Evalue=3e-21, Organism=Escherichia coli, GI1789057, Length=261, Percent_Identity=29.8850574712644, Blast_Score=94, Evalue=9e-21, Organism=Escherichia coli, GI2367175, Length=257, Percent_Identity=28.0155642023346, Blast_Score=78, Evalue=5e-16, Organism=Escherichia coli, GI1787820, Length=182, Percent_Identity=29.1208791208791, Blast_Score=74, Evalue=1e-14, Organism=Escherichia coli, GI2367365, Length=251, Percent_Identity=28.2868525896414, Blast_Score=70, Evalue=1e-13, Organism=Caenorhabditis elegans, GI17561402, Length=257, Percent_Identity=33.4630350194552, Blast_Score=125, Evalue=2e-29, Organism=Caenorhabditis elegans, GI25147288, Length=253, Percent_Identity=32.8063241106719, Blast_Score=123, Evalue=9e-29, Organism=Caenorhabditis elegans, GI71994600, Length=256, Percent_Identity=31.640625, Blast_Score=112, Evalue=2e-25, Organism=Caenorhabditis elegans, GI17555706, Length=261, Percent_Identity=31.0344827586207, Blast_Score=112, Evalue=2e-25, Organism=Caenorhabditis elegans, GI17562906, Length=267, Percent_Identity=29.5880149812734, Blast_Score=109, Evalue=1e-24, Organism=Caenorhabditis elegans, GI17560332, Length=275, Percent_Identity=29.8181818181818, Blast_Score=105, Evalue=2e-23, Organism=Caenorhabditis elegans, GI17560220, Length=274, Percent_Identity=28.4671532846715, Blast_Score=104, Evalue=4e-23, Organism=Caenorhabditis elegans, GI71994604, Length=255, Percent_Identity=30.9803921568627, Blast_Score=103, Evalue=7e-23, Organism=Caenorhabditis elegans, GI17538480, Length=262, Percent_Identity=31.2977099236641, Blast_Score=103, Evalue=7e-23, Organism=Caenorhabditis elegans, GI17560676, Length=258, Percent_Identity=31.0077519379845, Blast_Score=100, Evalue=5e-22, Organism=Caenorhabditis elegans, GI193204405, Length=274, Percent_Identity=27.3722627737226, Blast_Score=97, Evalue=7e-21, Organism=Caenorhabditis elegans, GI17562904, Length=271, Percent_Identity=27.3062730627306, Blast_Score=97, Evalue=7e-21, Organism=Caenorhabditis elegans, GI72000259, Length=266, Percent_Identity=28.9473684210526, Blast_Score=97, Evalue=8e-21, Organism=Caenorhabditis elegans, GI17562908, Length=267, Percent_Identity=27.7153558052434, Blast_Score=97, Evalue=1e-20, Organism=Caenorhabditis elegans, GI17562910, Length=269, Percent_Identity=28.2527881040892, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI17531453, Length=274, Percent_Identity=26.6423357664234, Blast_Score=94, Evalue=5e-20, Organism=Caenorhabditis elegans, GI17562990, Length=271, Percent_Identity=27.3062730627306, Blast_Score=94, Evalue=5e-20, Organism=Caenorhabditis elegans, GI17560150, Length=272, Percent_Identity=27.2058823529412, Blast_Score=93, Evalue=1e-19, Organism=Caenorhabditis elegans, GI17551412, Length=268, Percent_Identity=29.4776119402985, Blast_Score=93, Evalue=1e-19, Organism=Caenorhabditis elegans, GI17563726, Length=266, Percent_Identity=27.0676691729323, Blast_Score=92, Evalue=2e-19, Organism=Caenorhabditis elegans, GI17544670, Length=267, Percent_Identity=27.3408239700375, Blast_Score=91, Evalue=4e-19, Organism=Caenorhabditis elegans, GI17559104, Length=269, Percent_Identity=28.2527881040892, Blast_Score=91, Evalue=7e-19, Organism=Caenorhabditis elegans, GI115534694, Length=252, Percent_Identity=30.952380952381, Blast_Score=90, Evalue=1e-18, Organism=Caenorhabditis elegans, GI17564282, Length=198, Percent_Identity=29.2929292929293, Blast_Score=86, Evalue=2e-17, Organism=Caenorhabditis elegans, GI17538486, Length=268, Percent_Identity=28.3582089552239, Blast_Score=85, Evalue=3e-17, Organism=Caenorhabditis elegans, GI17565030, Length=273, Percent_Identity=28.9377289377289, Blast_Score=82, Evalue=2e-16, Organism=Caenorhabditis elegans, GI17508651, Length=254, Percent_Identity=29.1338582677165, Blast_Score=80, Evalue=1e-15, Organism=Caenorhabditis elegans, GI193203117, Length=194, Percent_Identity=29.3814432989691, Blast_Score=79, Evalue=2e-15, Organism=Caenorhabditis elegans, GI193203115, Length=194, Percent_Identity=29.3814432989691, Blast_Score=79, Evalue=2e-15, Organism=Caenorhabditis elegans, GI17538182, Length=267, Percent_Identity=27.7153558052434, Blast_Score=74, Evalue=8e-14, Organism=Caenorhabditis elegans, GI17508895, Length=192, Percent_Identity=26.5625, Blast_Score=72, Evalue=2e-13, Organism=Caenorhabditis elegans, GI17568967, Length=218, Percent_Identity=31.1926605504587, Blast_Score=71, Evalue=5e-13, Organism=Caenorhabditis elegans, GI17567345, Length=193, Percent_Identity=24.8704663212435, Blast_Score=66, Evalue=2e-11, Organism=Caenorhabditis elegans, GI17536025, Length=255, Percent_Identity=21.1764705882353, Blast_Score=65, Evalue=3e-11, Organism=Saccharomyces cerevisiae, GI6322861, Length=194, Percent_Identity=31.9587628865979, Blast_Score=85, Evalue=9e-18, Organism=Saccharomyces cerevisiae, GI6323882, Length=193, Percent_Identity=34.1968911917098, Blast_Score=76, Evalue=7e-15, Organism=Saccharomyces cerevisiae, GI6324126, Length=261, Percent_Identity=25.2873563218391, Blast_Score=73, Evalue=5e-14, Organism=Drosophila melanogaster, GI24639444, Length=253, Percent_Identity=33.9920948616601, Blast_Score=119, Evalue=2e-27, Organism=Drosophila melanogaster, GI23397609, Length=258, Percent_Identity=33.7209302325581, Blast_Score=115, Evalue=4e-26, Organism=Drosophila melanogaster, GI21355319, Length=252, Percent_Identity=32.5396825396825, Blast_Score=113, Evalue=1e-25, Organism=Drosophila melanogaster, GI21357041, Length=258, Percent_Identity=31.3953488372093, Blast_Score=112, Evalue=2e-25, Organism=Drosophila melanogaster, GI28571526, Length=262, Percent_Identity=32.824427480916, Blast_Score=107, Evalue=5e-24, Organism=Drosophila melanogaster, GI24644339, Length=256, Percent_Identity=29.6875, Blast_Score=97, Evalue=1e-20, Organism=Drosophila melanogaster, GI24643142, Length=249, Percent_Identity=30.5220883534137, Blast_Score=93, Evalue=1e-19, Organism=Drosophila melanogaster, GI17737361, Length=268, Percent_Identity=28.3582089552239, Blast_Score=92, Evalue=3e-19, Organism=Drosophila melanogaster, GI24644337, Length=260, Percent_Identity=31.5384615384615, Blast_Score=86, Evalue=2e-17, Organism=Drosophila melanogaster, GI281364892, Length=200, Percent_Identity=30, Blast_Score=84, Evalue=8e-17, Organism=Drosophila melanogaster, GI24640492, Length=189, Percent_Identity=26.4550264550265, Blast_Score=74, Evalue=1e-13, Organism=Drosophila melanogaster, GI24665264, Length=199, Percent_Identity=27.6381909547739, Blast_Score=71, Evalue=8e-13, Organism=Drosophila melanogaster, GI221331218, Length=194, Percent_Identity=28.8659793814433, Blast_Score=70, Evalue=2e-12, Organism=Drosophila melanogaster, GI24665243, Length=167, Percent_Identity=29.3413173652695, Blast_Score=70, Evalue=2e-12, Organism=Drosophila melanogaster, GI116007236, Length=253, Percent_Identity=25.296442687747, Blast_Score=70, Evalue=2e-12, Organism=Drosophila melanogaster, GI221331216, Length=195, Percent_Identity=28.7179487179487, Blast_Score=67, Evalue=1e-11, Organism=Drosophila melanogaster, GI24665247, Length=168, Percent_Identity=29.1666666666667, Blast_Score=67, Evalue=1e-11, Organism=Drosophila melanogaster, GI24649181, Length=189, Percent_Identity=25.9259259259259, Blast_Score=67, Evalue=2e-11, Organism=Drosophila melanogaster, GI221510726, Length=200, Percent_Identity=27, Blast_Score=66, Evalue=2e-11, Organism=Drosophila melanogaster, GI24581726, Length=199, Percent_Identity=28.1407035175879, Blast_Score=66, Evalue=2e-11, Organism=Drosophila melanogaster, GI18859837, Length=189, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=6e-11, Organism=Drosophila melanogaster, GI24641232, Length=200, Percent_Identity=26.5, Blast_Score=65, Evalue=6e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: =1.1.1.14 [H]
Molecular weight: Translated: 27335; Mature: 27335
Theoretical pI: Translated: 5.37; Mature: 5.37
Prosite motif: PS00061 ADH_SHORT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 3.5 %Met (Translated Protein) 4.3 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 3.5 %Met (Mature Protein) 4.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQDKVAILTGAASGIGEAVAQRYLEAGARCVLVDLKPAGGTLAQLIETHPDRAFALSADV CCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCCCCEEEEECCC TKRDDIERIVSAAVERFGGIDILFNNAAAFDMRPLLDEAWEVFDRLFAVNVKGMFFLMQA CCHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHH VAQRMAAQGRGGKIINMASQAGRRGEALVSHYCATKAAVISYTQSAALALAPYKINVNGI HHHHHHHCCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEECCC APGVVDTPMWEQVDALFARYENRPLGEKKRLVGEAVPLGRMGVPADLTGAALFLASTDAD CCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCC YITAQTLNVDGGNWMS EEEEEEEECCCCCCCC >Mature Secondary Structure MQDKVAILTGAASGIGEAVAQRYLEAGARCVLVDLKPAGGTLAQLIETHPDRAFALSADV CCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCCCCEEEEECCC TKRDDIERIVSAAVERFGGIDILFNNAAAFDMRPLLDEAWEVFDRLFAVNVKGMFFLMQA CCHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHH VAQRMAAQGRGGKIINMASQAGRRGEALVSHYCATKAAVISYTQSAALALAPYKINVNGI HHHHHHHCCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEECCC APGVVDTPMWEQVDALFARYENRPLGEKKRLVGEAVPLGRMGVPADLTGAALFLASTDAD CCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCC YITAQTLNVDGGNWMS EEEEEEEECCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 7551049 [H]