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The map label for this gene is nuoM [H]

Identifier: 17546769

GI number: 17546769

Start: 2222690

End: 2224156

Strand: Reverse

Name: nuoM [H]

Synonym: RSc2050

Alternate gene names: 17546769

Gene position: 2224156-2222690 (Counterclockwise)

Preceding gene: 17546770

Following gene: 17546768

Centisome position: 59.85

GC content: 61.83

Gene sequence:

>1467_bases
ATGGTTCTGTCTTTTGCGATCTGGCTGCCGATCTTTTTTGGCGTGCTGGTGCTGGCTTCGGGCTCAGACCGCAATCCCGG
CTATGTCCGCTGGATGTCGCTGATCGGCTCGCTGGTCAGCTTCGTGATCACGCTGCCGCTGATCACGCGCTTCGACAAGT
CGACCGCGGCGATGCAGTTCGTCGAGAAGTCGACCTGGATCGAGCGCTTCCACATCAGCTACTACCTCGGGGTCGACGGC
ATCTCGATGTGGTTCGTGGTGCTGACGGCGTTCATCACGGTGATTGTCGTGATCTCGGCGTGGGAAGTGATCACCGAGCG
TGTCGCGCAGTACATGGCGGCGTTCCTGATCCTGTCGGGCCTGATGATCGGCGTGTTCGCGGCGCTCGACGGCCTGCTGT
TCTACGTGTTCTTCGAGGCCACGCTGATCCCGATGTACATCATCATCGGTGTGTGGGGCGGCCCGAACCGCGTGTACGCG
GCCATCAAGTTCTTCCTGTACACGCTGCTGGGCTCGCTGCTGACGCTGGTTGCGTTCCTGTACCTGTACTTCCACAGCGG
CACGTTCGAGATCCTGCAATGGCACCAGTTGAAGCTGTCGCTGAACGAGCAGATCCTGCTGTTCATCGCCTTCTTCATGG
CGTTTGCCGTGAAGGTGCCGATGTGGCCGGTGCACACCTGGCTGCCGGATGCCCACGTGGAAGCGCCGACCGGCGGTTCG
GTGGTGCTGGCCGCCATCATGCTGAAGCTGGGCGCGTACGGTTTCCTGCGGTTCTCGCTGCCGATCGCGCCGGACGCGAG
CCATAGCCTGGCGGCCTTCGTCATCGCGATTTCGCTGATCGCCGTGATCTACATCGGCCTGGTGGCGCTGGTGCAGGCCG
ACATGAAGAAGCTGGTGGCGTATTCCTCGATCGCGCACATGGGCTTCGTCACGCTGGGCTTCTTCATCTTCAACGAGATC
GGCGTCGAGGGCGGCATCGTGCAGATGATCTCGCACGGCTTCATCTCGGGCGCGATGTTCCTGTGCATCGGCGTGCTGTA
CGACCGCGTGCACTCGCGCCAGATCGCCGACTACGGCGGTGTGGTGAACACGATGCCGAAGTTCGCGGCGCTGTCGGTGT
TCTTCGCCATGGCCAACTGCGGCCTGCCGGCCACCTCCGGTTTCGTGGGCGAGTTCATGGTGATCCTGGGTTCGGTCAAG
TTCAACTTCTGGATCGGCCTGCTGGCGGCCACCGCGCTGATCTTCGGCGCAGCGTACTCGCTGTGGATGGTCAAGCGCGT
GATCTTCGGCGACGTGGCGAACAAGCACGTGGCCGAGCTGCAGGACCTGAATGCGCGCGAATTCTTCATGCTGGGCGTAC
TCGCGATCGCGACCCTGTACATGGGCCTGTATCCGAAGCCTTTCACCGATGTCATGCATGCGTCCGTGGTGAATCTCCTC
ACCCACGTGGCCCAGACCAAGCTGTAA

Upstream 100 bases:

>100_bases
TCCGGCTACATCTATGACTATGCGTTCGCGATGATCGTCGGCACCGTGCTGCTGCTGACGTACTTCGTGTTCCTGGCGGG
CAAATAAGGGATAACGAAAA

Downstream 100 bases:

>100_bases
GCGGGGAGTCGAAACGATCATGCAAAACATGAACTGGGCACCGTTGTTGCCGATTATTTTCCTGTCGGTGATGATCGCCG
TGATCCAACTGGCCGACGTG

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MVLSFAIWLPIFFGVLVLASGSDRNPGYVRWMSLIGSLVSFVITLPLITRFDKSTAAMQFVEKSTWIERFHISYYLGVDG
ISMWFVVLTAFITVIVVISAWEVITERVAQYMAAFLILSGLMIGVFAALDGLLFYVFFEATLIPMYIIIGVWGGPNRVYA
AIKFFLYTLLGSLLTLVAFLYLYFHSGTFEILQWHQLKLSLNEQILLFIAFFMAFAVKVPMWPVHTWLPDAHVEAPTGGS
VVLAAIMLKLGAYGFLRFSLPIAPDASHSLAAFVIAISLIAVIYIGLVALVQADMKKLVAYSSIAHMGFVTLGFFIFNEI
GVEGGIVQMISHGFISGAMFLCIGVLYDRVHSRQIADYGGVVNTMPKFAALSVFFAMANCGLPATSGFVGEFMVILGSVK
FNFWIGLLAATALIFGAAYSLWMVKRVIFGDVANKHVAELQDLNAREFFMLGVLAIATLYMGLYPKPFTDVMHASVVNLL
THVAQTKL

Sequences:

>Translated_488_residues
MVLSFAIWLPIFFGVLVLASGSDRNPGYVRWMSLIGSLVSFVITLPLITRFDKSTAAMQFVEKSTWIERFHISYYLGVDG
ISMWFVVLTAFITVIVVISAWEVITERVAQYMAAFLILSGLMIGVFAALDGLLFYVFFEATLIPMYIIIGVWGGPNRVYA
AIKFFLYTLLGSLLTLVAFLYLYFHSGTFEILQWHQLKLSLNEQILLFIAFFMAFAVKVPMWPVHTWLPDAHVEAPTGGS
VVLAAIMLKLGAYGFLRFSLPIAPDASHSLAAFVIAISLIAVIYIGLVALVQADMKKLVAYSSIAHMGFVTLGFFIFNEI
GVEGGIVQMISHGFISGAMFLCIGVLYDRVHSRQIADYGGVVNTMPKFAALSVFFAMANCGLPATSGFVGEFMVILGSVK
FNFWIGLLAATALIFGAAYSLWMVKRVIFGDVANKHVAELQDLNAREFFMLGVLAIATLYMGLYPKPFTDVMHASVVNLL
THVAQTKL
>Mature_488_residues
MVLSFAIWLPIFFGVLVLASGSDRNPGYVRWMSLIGSLVSFVITLPLITRFDKSTAAMQFVEKSTWIERFHISYYLGVDG
ISMWFVVLTAFITVIVVISAWEVITERVAQYMAAFLILSGLMIGVFAALDGLLFYVFFEATLIPMYIIIGVWGGPNRVYA
AIKFFLYTLLGSLLTLVAFLYLYFHSGTFEILQWHQLKLSLNEQILLFIAFFMAFAVKVPMWPVHTWLPDAHVEAPTGGS
VVLAAIMLKLGAYGFLRFSLPIAPDASHSLAAFVIAISLIAVIYIGLVALVQADMKKLVAYSSIAHMGFVTLGFFIFNEI
GVEGGIVQMISHGFISGAMFLCIGVLYDRVHSRQIADYGGVVNTMPKFAALSVFFAMANCGLPATSGFVGEFMVILGSVK
FNFWIGLLAATALIFGAAYSLWMVKRVIFGDVANKHVAELQDLNAREFFMLGVLAIATLYMGLYPKPFTDVMHASVVNLL
THVAQTKL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=349, Percent_Identity=35.5300859598854, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI251831117, Length=466, Percent_Identity=24.2489270386266, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1788613, Length=468, Percent_Identity=38.034188034188, Blast_Score=314, Evalue=1e-86,
Organism=Escherichia coli, GI1788827, Length=284, Percent_Identity=25.3521126760563, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1788829, Length=469, Percent_Identity=27.0788912579957, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788831, Length=276, Percent_Identity=26.0869565217391, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI145693160, Length=358, Percent_Identity=24.3016759776536, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788614, Length=352, Percent_Identity=25.8522727272727, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53849; Mature: 53849

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCC
>Mature Secondary Structure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HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]