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The map label for this gene is yfcG [H]

Identifier: 17546740

GI number: 17546740

Start: 2199521

End: 2200213

Strand: Direct

Name: yfcG [H]

Synonym: RSc2021

Alternate gene names: 17546740

Gene position: 2199521-2200213 (Clockwise)

Preceding gene: 17546739

Following gene: 17546741

Centisome position: 59.18

GC content: 64.65

Gene sequence:

>693_bases
ATGATCGACGTCTACAGCTGGGCCACACCCAACGGGCACAAGGTTCACATCATGCTGGAGGAATGCGGACTGCCCTACCG
CGTCCATGCCGTCAACATCGGCGCTGGCGACCAGTTCAAGCCCGAGTTCCTGAAGATCAGCCCGAACAACAAGATTCCGG
CCATCGTCGACCAGGACGGACCGGACGGCCAGCCGATCTCGCTGTTCGAGTCGGGGGCCATCCTGCTGTACCTCGCCGGC
AAAACCGGCAAGTTCCTGCCCGAGGACGTGCGCGGCAAGTACGAGGTGCTGCAATGGCTGATGTTCCAGATGGGCGGCGT
CGGCCCGATGCTCGGCCAGACGCACCACTTCCGCATCTACGCGCCCGAGAAGATCGACTACGCGATCAACCGCTATACCA
ACGAGGCCAAGCGCCTGTATGGCGTGATCGACAAGCGCCTGTCGGAATCCAAGTACCTGGGCGGCCCGGACTACTCCATC
GCCGACATCGCCACCTGGCCGTGGCTGCGCAGCTGGCAGAACCAGGGGATCGTGCTGTCGGACTACCCCAGGCTGCAGCG
CTGGTTCGAGGCCATCGAGGCGCGCCCCGCCGTGCAGCGCGCGGTGAAGGTGCTGGCCAAGGAACGCTCGCCCCTGCAGG
ACGACAAAGAACGCGAGGTGCTGTTCGGCGCGACGCAATACGCGCGCCGCTAG

Upstream 100 bases:

>100_bases
TTTTGCGCGCGCGGGCTGTCTGCGCGTTTTCGCGTTCTGTGCTTGAATGGGCGGCTTGCAACGTCTCCCATCTCTCTTTT
CCGCTCAGGAGCACCAACTC

Downstream 100 bases:

>100_bases
GCAGCGCTGCTGCACAGGGAAAGGGGCCGCGTCGTGCGGCCCTTTTTGCTTGGGGCGCCCCCCTGCGGAAACCGCCGGCC
CCGCCCGCGCCGCCTACACT

Product: GST-related protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MIDVYSWATPNGHKVHIMLEECGLPYRVHAVNIGAGDQFKPEFLKISPNNKIPAIVDQDGPDGQPISLFESGAILLYLAG
KTGKFLPEDVRGKYEVLQWLMFQMGGVGPMLGQTHHFRIYAPEKIDYAINRYTNEAKRLYGVIDKRLSESKYLGGPDYSI
ADIATWPWLRSWQNQGIVLSDYPRLQRWFEAIEARPAVQRAVKVLAKERSPLQDDKEREVLFGATQYARR

Sequences:

>Translated_230_residues
MIDVYSWATPNGHKVHIMLEECGLPYRVHAVNIGAGDQFKPEFLKISPNNKIPAIVDQDGPDGQPISLFESGAILLYLAG
KTGKFLPEDVRGKYEVLQWLMFQMGGVGPMLGQTHHFRIYAPEKIDYAINRYTNEAKRLYGVIDKRLSESKYLGGPDYSI
ADIATWPWLRSWQNQGIVLSDYPRLQRWFEAIEARPAVQRAVKVLAKERSPLQDDKEREVLFGATQYARR
>Mature_230_residues
MIDVYSWATPNGHKVHIMLEECGLPYRVHAVNIGAGDQFKPEFLKISPNNKIPAIVDQDGPDGQPISLFESGAILLYLAG
KTGKFLPEDVRGKYEVLQWLMFQMGGVGPMLGQTHHFRIYAPEKIDYAINRYTNEAKRLYGVIDKRLSESKYLGGPDYSI
ADIATWPWLRSWQNQGIVLSDYPRLQRWFEAIEARPAVQRAVKVLAKERSPLQDDKEREVLFGATQYARR

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=213, Percent_Identity=57.2769953051643, Blast_Score=242, Evalue=1e-65,
Organism=Escherichia coli, GI87082195, Length=227, Percent_Identity=43.6123348017621, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI87081789, Length=204, Percent_Identity=27.9411764705882, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324100, Length=235, Percent_Identity=32.7659574468085, Blast_Score=126, Evalue=4e-30,
Organism=Drosophila melanogaster, GI17933730, Length=228, Percent_Identity=26.3157894736842, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17864598, Length=206, Percent_Identity=30.0970873786408, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28571670, Length=175, Percent_Identity=26.2857142857143, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17864592, Length=195, Percent_Identity=28.7179487179487, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646249, Length=163, Percent_Identity=29.4478527607362, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922160, Length=215, Percent_Identity=25.1162790697674, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI85725204, Length=194, Percent_Identity=26.8041237113402, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17737923, Length=194, Percent_Identity=26.8041237113402, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17864594, Length=192, Percent_Identity=27.6041666666667, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20130115, Length=198, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26252; Mature: 26252

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVYSWATPNGHKVHIMLEECGLPYRVHAVNIGAGDQFKPEFLKISPNNKIPAIVDQDG
CCCEEECCCCCCCEEEEEEHHCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCC
PDGQPISLFESGAILLYLAGKTGKFLPEDVRGKYEVLQWLMFQMGGVGPMLGQTHHFRIY
CCCCCEEEECCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEEE
APEKIDYAINRYTNEAKRLYGVIDKRLSESKYLGGPDYSIADIATWPWLRSWQNQGIVLS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCHHHHHHCCCCEEEE
DYPRLQRWFEAIEARPAVQRAVKVLAKERSPLQDDKEREVLFGATQYARR
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHEEECCHHHHCC
>Mature Secondary Structure
MIDVYSWATPNGHKVHIMLEECGLPYRVHAVNIGAGDQFKPEFLKISPNNKIPAIVDQDG
CCCEEECCCCCCCEEEEEEHHCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCC
PDGQPISLFESGAILLYLAGKTGKFLPEDVRGKYEVLQWLMFQMGGVGPMLGQTHHFRIY
CCCCCEEEECCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEEE
APEKIDYAINRYTNEAKRLYGVIDKRLSESKYLGGPDYSIADIATWPWLRSWQNQGIVLS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCHHHHHHCCCCEEEE
DYPRLQRWFEAIEARPAVQRAVKVLAKERSPLQDDKEREVLFGATQYARR
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]