Click here to switch to the map view.

The map label for this gene is mdh

Identifier: 17546717

GI number: 17546717

Start: 2174783

End: 2175772

Strand: Direct

Name: mdh

Synonym: RSc1998

Alternate gene names: 17546717

Gene position: 2174783-2175772 (Clockwise)

Preceding gene: 17546694

Following gene: 17546718

Centisome position: 58.52

GC content: 65.05

Gene sequence:

>990_bases
ATGGCTAAAGCACCCATGCGCGTCGCGGTGACCGGCGCTGCTGGTCAGATCGGTTATGCACTGCTGTTCCGCATCGCCGC
CGGCGAAATGCTGGGCAAGGATCAACCCGTCATCCTGCAACTGCTGGAAATCCCCGACGAGAAGGCCCAGAAGGCGCTCA
AGGGCGTGATGATGGAGATCGAAGACTGCGCCTTCCCGCTGCTGGCCGGCATGGAAGCCCACGCCGACCCGATGACCGCC
TTCAAGGACGTGGACGTGGCCCTGCTGGTCGGCGCCCGCCCCCGCGGCCCTGGCATGGAGCGCAAGGACCTGCTGTCGGC
CAACGCACAGATCTTCACGGCACAGGGCAAGGCCCTGAACGCCGTCGCTTCGCGCAACGTCAAGGTGCTGGTGGTCGGCA
ACCCGGCCAACACCAACGCCTACATCGCCATGAAGTCGGCACCGGACCTGCCGCGCGAGAACTTCACCGCGATGCTGCGC
CTGGACCACAACCGCGCCCTGTCGCAGATCGCCGCCAAGACCGGCAAGCCGGTGTCGAGCATCGAAAAGCTGTTCGTGTG
GGGCAACCACAGCCCGACGATGTACGCCGACTACCGCTACGCCACTATCGACGGCCAGAGCGTGAAGGACATGATCAACG
ACCCGGTGTGGAACAACGACGTGTTCCTGCCGACCGTCGGCAAGCGCGGCGCCGCCATCATCGAGGCGCGCGGCCTATCG
TCGGCTGCCTCGGCCGCCAACGCCGCCATCGACCACGTGCGCGACTGGGTACTGGGCTCCAACGGCAAGATCGTCACGAT
GGGCATCCCGTCCAACGGCGACTACGAGATCCCGCAGGACGTGATGTTCGGCTTCCCGGTCACGACGGCCAACGGCAAGT
ACGAAGTGGTCAAGGGCTTCGAGGTCGACGCCTACAGCCGCGAGAAGATCAACATCACGCTCAAGGAACTGGAAGAAGAG
CGTGCCGGCGTGCAGCACCTGCTGGGCTGA

Upstream 100 bases:

>100_bases
CGCCCGGACCGGCAATGCCTGCGCAGTGGGCGCCGACATCCCGCCAGGATGCGACTTCGCGCAGGGCGATCCGCTTTTCA
CTACCGTTTGGAGAGTTCTC

Downstream 100 bases:

>100_bases
CGCGCATGATCTGACGAGAAACCGGAGCGGATCGGCAGCGAACCCTCCGGTTTTTTTACATCCCAACCCGGCGCCGGAAA
CGTCCGCGCCCCCACCCGAT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MAKAPMRVAVTGAAGQIGYALLFRIAAGEMLGKDQPVILQLLEIPDEKAQKALKGVMMEIEDCAFPLLAGMEAHADPMTA
FKDVDVALLVGARPRGPGMERKDLLSANAQIFTAQGKALNAVASRNVKVLVVGNPANTNAYIAMKSAPDLPRENFTAMLR
LDHNRALSQIAAKTGKPVSSIEKLFVWGNHSPTMYADYRYATIDGQSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLS
SAASAANAAIDHVRDWVLGSNGKIVTMGIPSNGDYEIPQDVMFGFPVTTANGKYEVVKGFEVDAYSREKINITLKELEEE
RAGVQHLLG

Sequences:

>Translated_329_residues
MAKAPMRVAVTGAAGQIGYALLFRIAAGEMLGKDQPVILQLLEIPDEKAQKALKGVMMEIEDCAFPLLAGMEAHADPMTA
FKDVDVALLVGARPRGPGMERKDLLSANAQIFTAQGKALNAVASRNVKVLVVGNPANTNAYIAMKSAPDLPRENFTAMLR
LDHNRALSQIAAKTGKPVSSIEKLFVWGNHSPTMYADYRYATIDGQSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLS
SAASAANAAIDHVRDWVLGSNGKIVTMGIPSNGDYEIPQDVMFGFPVTTANGKYEVVKGFEVDAYSREKINITLKELEEE
RAGVQHLLG
>Mature_328_residues
AKAPMRVAVTGAAGQIGYALLFRIAAGEMLGKDQPVILQLLEIPDEKAQKALKGVMMEIEDCAFPLLAGMEAHADPMTAF
KDVDVALLVGARPRGPGMERKDLLSANAQIFTAQGKALNAVASRNVKVLVVGNPANTNAYIAMKSAPDLPRENFTAMLRL
DHNRALSQIAAKTGKPVSSIEKLFVWGNHSPTMYADYRYATIDGQSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLSS
AASAANAAIDHVRDWVLGSNGKIVTMGIPSNGDYEIPQDVMFGFPVTTANGKYEVVKGFEVDAYSREKINITLKELEEER
AGVQHLLG

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=330, Percent_Identity=49.3939393939394, Blast_Score=319, Evalue=2e-87,
Organism=Homo sapiens, GI89886456, Length=313, Percent_Identity=24.9201277955272, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17561064, Length=324, Percent_Identity=50.3086419753086, Blast_Score=308, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI32566798, Length=262, Percent_Identity=51.1450381679389, Blast_Score=248, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI32566800, Length=169, Percent_Identity=45.5621301775148, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24583394, Length=322, Percent_Identity=53.1055900621118, Blast_Score=326, Evalue=2e-89,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_RALSO (Q8XXW5)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520119.1
- ProteinModelPortal:   Q8XXW5
- SMR:   Q8XXW5
- GeneID:   1220838
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1998
- NMPDR:   fig|267608.1.peg.1998
- HOGENOM:   HBG289884
- OMA:   IEKLFVW
- ProtClustDB:   PRK05442
- BioCyc:   RSOL267608:RSC1998-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 35423; Mature: 35292

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 190-190 BINDING 95-95 BINDING 101-101 BINDING 108-108 BINDING 115-115 BINDING 134-134 BINDING 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAPMRVAVTGAAGQIGYALLFRIAAGEMLGKDQPVILQLLEIPDEKAQKALKGVMMEI
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
EDCAFPLLAGMEAHADPMTAFKDVDVALLVGARPRGPGMERKDLLSANAQIFTAQGKALN
HHHHHHHHCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCHHHHHCCCCEEEEECCCEEH
AVASRNVKVLVVGNPANTNAYIAMKSAPDLPRENFTAMLRLDHNRALSQIAAKTGKPVSS
HHHCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHH
IEKLFVWGNHSPTMYADYRYATIDGQSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLS
HEEEEEEECCCCCEEEEEEEEEECCHHHHHHHCCCCCCCCEEEECCCCCCCEEEEECCCC
SAASAANAAIDHVRDWVLGSNGKIVTMGIPSNGDYEIPQDVMFGFPVTTANGKYEVVKGF
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCEEEECCCCEEEEECE
EVDAYSREKINITLKELEEERAGVQHLLG
EECCCCCCEEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKAPMRVAVTGAAGQIGYALLFRIAAGEMLGKDQPVILQLLEIPDEKAQKALKGVMMEI
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
EDCAFPLLAGMEAHADPMTAFKDVDVALLVGARPRGPGMERKDLLSANAQIFTAQGKALN
HHHHHHHHCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCHHHHHCCCCEEEEECCCEEH
AVASRNVKVLVVGNPANTNAYIAMKSAPDLPRENFTAMLRLDHNRALSQIAAKTGKPVSS
HHHCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHH
IEKLFVWGNHSPTMYADYRYATIDGQSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLS
HEEEEEEECCCCCEEEEEEEEEECCHHHHHHHCCCCCCCCEEEECCCCCCCEEEEECCCC
SAASAANAAIDHVRDWVLGSNGKIVTMGIPSNGDYEIPQDVMFGFPVTTANGKYEVVKGF
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCEEEECCCCEEEEECE
EVDAYSREKINITLKELEEERAGVQHLLG
EECCCCCCEEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852