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The map label for this gene is metG2 [H]

Identifier: 17546537

GI number: 17546537

Start: 1987271

End: 1988761

Strand: Direct

Name: metG2 [H]

Synonym: RSc1818

Alternate gene names: 17546537

Gene position: 1987271-1988761 (Clockwise)

Preceding gene: 17546536

Following gene: 17546538

Centisome position: 53.47

GC content: 65.26

Gene sequence:

>1491_bases
ATGAAAGCTCGCAAAACGTACGTGCTCATTCCGGTGATGCCGACACCGAATGGCCCGCTGCATCTTGGCCACATCGCGGG
CCCGTTCCTCAAGATGGACATGCTCGCGCGGCATCTGCGGCGCAATGGCAACACGGTCGCGCTGGTCTCCGCGACGGATC
CTTATGAGACCCATGTGCTGCCCCGCGCCGACGAACAGAACAAGCCCGTCGAGCAGATCTGCGCCGAAAACCATCGGGCG
ATTCACCGTTGCCTGCAGGCACTCGACATTCGCTACGATGCTTTTATCGATCCGCTCGCATCGCCGTACCGGGCACGCCT
GAACGGCATTACGCGCGAAGTGCTCGACGACCTGCATGCGCAGGGACGGCTGCACGCGCGCAACGAGCCGGTCCACATCA
GCCGCCGAACCGGGCGGATGCTCGTCGGCAGCCGGATCGTCGGCACGTGTCCGTGTTGCGGCGTCGAAATGGGCGGCTAC
CATTGCGAAGGGTGCGGAATGGAAGTCTCGCCCCGCGACCTGATTGCCCCGCGCGCCGAACCGGCGGACGACACGGTTGA
GGTGGAAGCGCGGGCCAGTGTTTTCGTCGACGCCGATCTCCCCGCGCTCCAGCGGCGGATGCTCGAAGCGCGGGTGCCGG
CCGACGTCCGCCGCATCGCCGAACGGTTCATGCATGCAGCGGGCAGCGCGGTGCGGCTCTCGAACCCGGGCGAATGGGGC
GAGATCTGGCCCAATACGCTGGCAACCGCACCATCGGTGGTGTTCTCCTACACGGCGCTGTTCATGCTATCGGTACTGTG
CGGCGAGGCGGCGCGTGAAATCCTCGCGCTCGACCACAATCCGTTTGATCGGCGCTCCGACGCGCTGATCGTCACCAGCT
TCGGATTCGACAATACGGTGCCTTTCTGCGTGGGCGTGGAGACGCTCGCGCAACACAGCAGGCGCTACCGCGGCTTCGAC
CGCTGCTTGACGAACTTCTTCTACACGCTCGACGGCCGGAAGTTCTCGACGAGCCGCCAGCACTGCATCTGGGCCGACCA
GGCGGTACGGGAGCTGGGCGTGGCCTCGGACGTGCTGCGCTATTTCCTGGCCAAGACCAGCCCGGAAAGCGGACCGAGCG
ATTTCTCGCGCGACGGCTTCGACGCGTTCCGGCGCGCGATCGAACCCCGCCTGGCGCAGATGAAAGCCGCGGTTGAATCC
GGCATTGGCGGCGATGCAGCCGTTCCCTATGCGGACACATTGGCGCGACTTGTCGCGGACATGGACGCGGCTTTCGACGT
CGATCATTTCAGCCTGCGTGCCGCGACACGCTGCATTGATCAATGGCTCGCACTCCCATGGCAGCCGGCGCATGCCGCCG
CGTATGTGCGCGGCTTTTGCATGCTGGCCTATCCGGTCATGCCCGAGCTGGCCTCGACCCTGTGGAGCCAACTCGGCCAC
GAAGGCCTGCCTCGCTATCTAGCCGCGACCGAACTGGAGGAAGCCGCATGA

Upstream 100 bases:

>100_bases
CGCGGGTGGCATTCCAGACGTTCGGGGCCGGCGCGGAACAATCACCATCGACCGCCGGCACGACACCCAGCCCCTCACAT
CAGACCATCGAGGCGATTTG

Downstream 100 bases:

>100_bases
CACGTTCGCCCATCCGCCTGGCTTTCCAGGCAACGCAGGTCTCGGAGACGGGGCTGAGTGTCGATGAGATCGATTTCGGT
TGTTTTCGCGCGGGCGCGAC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MKARKTYVLIPVMPTPNGPLHLGHIAGPFLKMDMLARHLRRNGNTVALVSATDPYETHVLPRADEQNKPVEQICAENHRA
IHRCLQALDIRYDAFIDPLASPYRARLNGITREVLDDLHAQGRLHARNEPVHISRRTGRMLVGSRIVGTCPCCGVEMGGY
HCEGCGMEVSPRDLIAPRAEPADDTVEVEARASVFVDADLPALQRRMLEARVPADVRRIAERFMHAAGSAVRLSNPGEWG
EIWPNTLATAPSVVFSYTALFMLSVLCGEAAREILALDHNPFDRRSDALIVTSFGFDNTVPFCVGVETLAQHSRRYRGFD
RCLTNFFYTLDGRKFSTSRQHCIWADQAVRELGVASDVLRYFLAKTSPESGPSDFSRDGFDAFRRAIEPRLAQMKAAVES
GIGGDAAVPYADTLARLVADMDAAFDVDHFSLRAATRCIDQWLALPWQPAHAAAYVRGFCMLAYPVMPELASTLWSQLGH
EGLPRYLAATELEEAA

Sequences:

>Translated_496_residues
MKARKTYVLIPVMPTPNGPLHLGHIAGPFLKMDMLARHLRRNGNTVALVSATDPYETHVLPRADEQNKPVEQICAENHRA
IHRCLQALDIRYDAFIDPLASPYRARLNGITREVLDDLHAQGRLHARNEPVHISRRTGRMLVGSRIVGTCPCCGVEMGGY
HCEGCGMEVSPRDLIAPRAEPADDTVEVEARASVFVDADLPALQRRMLEARVPADVRRIAERFMHAAGSAVRLSNPGEWG
EIWPNTLATAPSVVFSYTALFMLSVLCGEAAREILALDHNPFDRRSDALIVTSFGFDNTVPFCVGVETLAQHSRRYRGFD
RCLTNFFYTLDGRKFSTSRQHCIWADQAVRELGVASDVLRYFLAKTSPESGPSDFSRDGFDAFRRAIEPRLAQMKAAVES
GIGGDAAVPYADTLARLVADMDAAFDVDHFSLRAATRCIDQWLALPWQPAHAAAYVRGFCMLAYPVMPELASTLWSQLGH
EGLPRYLAATELEEAA
>Mature_496_residues
MKARKTYVLIPVMPTPNGPLHLGHIAGPFLKMDMLARHLRRNGNTVALVSATDPYETHVLPRADEQNKPVEQICAENHRA
IHRCLQALDIRYDAFIDPLASPYRARLNGITREVLDDLHAQGRLHARNEPVHISRRTGRMLVGSRIVGTCPCCGVEMGGY
HCEGCGMEVSPRDLIAPRAEPADDTVEVEARASVFVDADLPALQRRMLEARVPADVRRIAERFMHAAGSAVRLSNPGEWG
EIWPNTLATAPSVVFSYTALFMLSVLCGEAAREILALDHNPFDRRSDALIVTSFGFDNTVPFCVGVETLAQHSRRYRGFD
RCLTNFFYTLDGRKFSTSRQHCIWADQAVRELGVASDVLRYFLAKTSPESGPSDFSRDGFDAFRRAIEPRLAQMKAAVES
GIGGDAAVPYADTLARLVADMDAAFDVDHFSLRAATRCIDQWLALPWQPAHAAAYVRGFCMLAYPVMPELASTLWSQLGH
EGLPRYLAATELEEAA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=399, Percent_Identity=22.8070175438596, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788432, Length=392, Percent_Identity=22.7040816326531, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6321704, Length=393, Percent_Identity=21.882951653944, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24655311, Length=401, Percent_Identity=23.4413965087282, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 54955; Mature: 54955

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKARKTYVLIPVMPTPNGPLHLGHIAGPFLKMDMLARHLRRNGNTVALVSATDPYETHVL
CCCCCEEEEEEEECCCCCCEEEHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCC
PRADEQNKPVEQICAENHRAIHRCLQALDIRYDAFIDPLASPYRARLNGITREVLDDLHA
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QGRLHARNEPVHISRRTGRMLVGSRIVGTCPCCGVEMGGYHCEGCGMEVSPRDLIAPRAE
CCCEECCCCCEEEECCCCCEEECCCHHCCCCCCCCCCCCEEECCCCCCCCCHHHCCCCCC
PADDTVEVEARASVFVDADLPALQRRMLEARVPADVRRIAERFMHAAGSAVRLSNPGEWG
CCCCCEEEEEEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCCH
EIWPNTLATAPSVVFSYTALFMLSVLCGEAAREILALDHNPFDRRSDALIVTSFGFDNTV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCC
PFCVGVETLAQHSRRYRGFDRCLTNFFYTLDGRKFSTSRQHCIWADQAVRELGVASDVLR
CHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEECCCCCCEEEHHHHHHHHCHHHHHHH
YFLAKTSPESGPSDFSRDGFDAFRRAIEPRLAQMKAAVESGIGGDAAVPYADTLARLVAD
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
MDAAFDVDHFSLRAATRCIDQWLALPWQPAHAAAYVRGFCMLAYPVMPELASTLWSQLGH
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EGLPRYLAATELEEAA
CCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKARKTYVLIPVMPTPNGPLHLGHIAGPFLKMDMLARHLRRNGNTVALVSATDPYETHVL
CCCCCEEEEEEEECCCCCCEEEHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCC
PRADEQNKPVEQICAENHRAIHRCLQALDIRYDAFIDPLASPYRARLNGITREVLDDLHA
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QGRLHARNEPVHISRRTGRMLVGSRIVGTCPCCGVEMGGYHCEGCGMEVSPRDLIAPRAE
CCCEECCCCCEEEECCCCCEEECCCHHCCCCCCCCCCCCEEECCCCCCCCCHHHCCCCCC
PADDTVEVEARASVFVDADLPALQRRMLEARVPADVRRIAERFMHAAGSAVRLSNPGEWG
CCCCCEEEEEEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCCH
EIWPNTLATAPSVVFSYTALFMLSVLCGEAAREILALDHNPFDRRSDALIVTSFGFDNTV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCC
PFCVGVETLAQHSRRYRGFDRCLTNFFYTLDGRKFSTSRQHCIWADQAVRELGVASDVLR
CHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEECCCCCCEEEHHHHHHHHCHHHHHHH
YFLAKTSPESGPSDFSRDGFDAFRRAIEPRLAQMKAAVESGIGGDAAVPYADTLARLVAD
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
MDAAFDVDHFSLRAATRCIDQWLALPWQPAHAAAYVRGFCMLAYPVMPELASTLWSQLGH
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EGLPRYLAATELEEAA
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA