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The map label for this gene is mdeA [H]

Identifier: 17546281

GI number: 17546281

Start: 1677026

End: 1678360

Strand: Reverse

Name: mdeA [H]

Synonym: RSc1562

Alternate gene names: 17546281

Gene position: 1678360-1677026 (Counterclockwise)

Preceding gene: 17546286

Following gene: 17546280

Centisome position: 45.16

GC content: 70.71

Gene sequence:

>1335_bases
ATGTCCAAAGCCCGCTTCGACACGCTGGCGCTGCATGCCGGTGCCGCGCCCGACCCGGCCACCGGCGCCCGCGCGACGCC
GATCCACCTCACCACCTCCTTCGTCTTCCGCGACAGCGAACACGCCGCCTCGCTGTTCAACATGGAGCGCGCGGGCCACG
TCTACTCGCGCATCTCCAACCCCACCGTGGCGGTGTTCGAGGAGCGCATGGCGGCGCTGGAGAACGGCGTCGGCGCGATC
GCCACGGCGTCGGGCCAGGCCGCGCTGCACCTGGCCATCGTCACGCTGATGGGCGCGGGCTCGCATATCGTGGCGTCGTC
GGCGCTGTACGGCGGCTCGCACAATCTGCTGCACTACACGCTGCGCCGCTTCGGCATCGAGACCACCTTCGTCAAGCCGG
GCGACATCGACGGCTGGCGCGCGGCCATCCGCCCCGAGACGCGCCTGCTGTTCGGCGAGACGCTGGGCAATCCGGGGCTG
GATGTGCTCGACATTCCGACCGTCGCATCGATCGCGCACGATGCCGGCGTGCCGCTGCTGGTCGATTCGACCTTTACCAC
GCCGTGGCTGCTGCAGCCGTTCGCGCACGGCGCCGACCTGCTCTACCACTCGGCCACCAAGTTCCTGGGCGGGCACGGCA
CCACCATCGGCGGCGTACTGGTCGACGCCGGCACCTTCGACTACGTGGCGGCCGGCCGGCATCCCGAGCTGACCGAGCCG
TACGCAGGCTTTCACGACATGGTGTTTGCCGAGGAAAGCACCGTGGCGCCCTTCCTGCTGCGCGCCCGCCGCGAGGGCCT
GCGCGACTTCGGCGCGTGCATGAACCCGATGGCGGCATGGCAATTGCTGCAAGGCGTCGAGACGCTGCCGCTGCGCATGG
CGCGGCACGTCGAGAACACGCGCCGCGTGGTCGCCTTCCTGGCGGGCCACCCCATGGTGGCGTCGGTGGCCTATCCCGAA
CTGGAAACGCACCCCGACCACGCGCTCGCCAAGCGCCTGCTGCCGCGCGGCTGTGGCGCGGTGTTCAGCTTCGATCTGCG
CGGCGACCGGCGCGCCGGCCAGCAATTCATCGAATCGCTCGGCCTGTTCTCGCACCTGGCCAATGTGGGCGACGCGCGCT
CGCTGGTGATCCACCCGGCCTCGACCACGCATTTCCGCATGGATGCGGCCGCGCTGGCCGCCGCCGGCATCGGCGAAGGC
ACGATCCGCCTGTCGATCGGCCTGGAGGACGCCGACGACCTGATCGACGATCTCAAGCGCGGTCTGAAGGCCGCCGAGCG
TGCCATGTCGGCCGCGTCCGGCAAGACCGCCCCCGCCCCGACCGGAGCCCGCTGA

Upstream 100 bases:

>100_bases
CCGTCAAAAACCGCGCCCGCTCGGCGTTTGCCCGGGATGCACCGCAACAGGGTGCGGTGTTAGCATGCCCCGTCCCGACC
TCATTTGGTGCTTGCTTCCC

Downstream 100 bases:

>100_bases
TGGAATGGACCGTACAAGGCCGGCGCGCCTATGCCTACACCGGCGGCAAGCCGTTCGATCCGGCGCTGCCATGCGTGGTC
TTCGTGCACGGCGCGCAGAA

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSKARFDTLALHAGAAPDPATGARATPIHLTTSFVFRDSEHAASLFNMERAGHVYSRISNPTVAVFEERMAALENGVGAI
ATASGQAALHLAIVTLMGAGSHIVASSALYGGSHNLLHYTLRRFGIETTFVKPGDIDGWRAAIRPETRLLFGETLGNPGL
DVLDIPTVASIAHDAGVPLLVDSTFTTPWLLQPFAHGADLLYHSATKFLGGHGTTIGGVLVDAGTFDYVAAGRHPELTEP
YAGFHDMVFAEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQGVETLPLRMARHVENTRRVVAFLAGHPMVASVAYPE
LETHPDHALAKRLLPRGCGAVFSFDLRGDRRAGQQFIESLGLFSHLANVGDARSLVIHPASTTHFRMDAAALAAAGIGEG
TIRLSIGLEDADDLIDDLKRGLKAAERAMSAASGKTAPAPTGAR

Sequences:

>Translated_444_residues
MSKARFDTLALHAGAAPDPATGARATPIHLTTSFVFRDSEHAASLFNMERAGHVYSRISNPTVAVFEERMAALENGVGAI
ATASGQAALHLAIVTLMGAGSHIVASSALYGGSHNLLHYTLRRFGIETTFVKPGDIDGWRAAIRPETRLLFGETLGNPGL
DVLDIPTVASIAHDAGVPLLVDSTFTTPWLLQPFAHGADLLYHSATKFLGGHGTTIGGVLVDAGTFDYVAAGRHPELTEP
YAGFHDMVFAEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQGVETLPLRMARHVENTRRVVAFLAGHPMVASVAYPE
LETHPDHALAKRLLPRGCGAVFSFDLRGDRRAGQQFIESLGLFSHLANVGDARSLVIHPASTTHFRMDAAALAAAGIGEG
TIRLSIGLEDADDLIDDLKRGLKAAERAMSAASGKTAPAPTGAR
>Mature_443_residues
SKARFDTLALHAGAAPDPATGARATPIHLTTSFVFRDSEHAASLFNMERAGHVYSRISNPTVAVFEERMAALENGVGAIA
TASGQAALHLAIVTLMGAGSHIVASSALYGGSHNLLHYTLRRFGIETTFVKPGDIDGWRAAIRPETRLLFGETLGNPGLD
VLDIPTVASIAHDAGVPLLVDSTFTTPWLLQPFAHGADLLYHSATKFLGGHGTTIGGVLVDAGTFDYVAAGRHPELTEPY
AGFHDMVFAEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQGVETLPLRMARHVENTRRVVAFLAGHPMVASVAYPEL
ETHPDHALAKRLLPRGCGAVFSFDLRGDRRAGQQFIESLGLFSHLANVGDARSLVIHPASTTHFRMDAAALAAAGIGEGT
IRLSIGLEDADDLIDDLKRGLKAAERAMSAASGKTAPAPTGAR

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=423, Percent_Identity=33.5697399527187, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI299473759, Length=423, Percent_Identity=30.7328605200946, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI25453487, Length=422, Percent_Identity=29.3838862559242, Blast_Score=136, Evalue=5e-32,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=33.3333333333333, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI1789383, Length=436, Percent_Identity=26.1467889908257, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71980708, Length=405, Percent_Identity=30.1234567901235, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17538055, Length=425, Percent_Identity=30.1176470588235, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17560056, Length=424, Percent_Identity=30.6603773584906, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=42.6605504587156, Blast_Score=309, Evalue=6e-85,
Organism=Saccharomyces cerevisiae, GI6319307, Length=428, Percent_Identity=32.7102803738318, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321254, Length=472, Percent_Identity=26.0593220338983, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321170, Length=354, Percent_Identity=26.271186440678, Blast_Score=116, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6321904, Length=429, Percent_Identity=27.5058275058275, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19922554, Length=427, Percent_Identity=31.8501170960187, Blast_Score=218, Evalue=7e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47171; Mature: 47040

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKARFDTLALHAGAAPDPATGARATPIHLTTSFVFRDSEHAASLFNMERAGHVYSRISN
CCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCC
PTVAVFEERMAALENGVGAIATASGQAALHLAIVTLMGAGSHIVASSALYGGSHNLLHYT
CHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHH
LRRFGIETTFVKPGDIDGWRAAIRPETRLLFGETLGNPGLDVLDIPTVASIAHDAGVPLL
HHHHCCCEEEECCCCCCCHHHHCCCCCEEEEEHHCCCCCCCEEECCHHHHHHHHCCCCEE
VDSTFTTPWLLQPFAHGADLLYHSATKFLGGHGTTIGGVLVDAGTFDYVAAGRHPELTEP
EECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEECCCCHHHCCCCCCCCCCC
YAGFHDMVFAEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQGVETLPLRMARHVENT
CCCHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHHHHHHHH
RRVVAFLAGHPMVASVAYPELETHPDHALAKRLLPRGCGAVFSFDLRGDRRAGQQFIESL
HHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
GLFSHLANVGDARSLVIHPASTTHFRMDAAALAAAGIGEGTIRLSIGLEDADDLIDDLKR
HHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
GLKAAERAMSAASGKTAPAPTGAR
HHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SKARFDTLALHAGAAPDPATGARATPIHLTTSFVFRDSEHAASLFNMERAGHVYSRISN
CCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCC
PTVAVFEERMAALENGVGAIATASGQAALHLAIVTLMGAGSHIVASSALYGGSHNLLHYT
CHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHH
LRRFGIETTFVKPGDIDGWRAAIRPETRLLFGETLGNPGLDVLDIPTVASIAHDAGVPLL
HHHHCCCEEEECCCCCCCHHHHCCCCCEEEEEHHCCCCCCCEEECCHHHHHHHHCCCCEE
VDSTFTTPWLLQPFAHGADLLYHSATKFLGGHGTTIGGVLVDAGTFDYVAAGRHPELTEP
EECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEECCCCHHHCCCCCCCCCCC
YAGFHDMVFAEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQGVETLPLRMARHVENT
CCCHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHHHHHHHH
RRVVAFLAGHPMVASVAYPELETHPDHALAKRLLPRGCGAVFSFDLRGDRRAGQQFIESL
HHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
GLFSHLANVGDARSLVIHPASTTHFRMDAAALAAAGIGEGTIRLSIGLEDADDLIDDLKR
HHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
GLKAAERAMSAASGKTAPAPTGAR
HHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]