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The map label for this gene is carA

Identifier: 17546238

GI number: 17546238

Start: 1621137

End: 1622273

Strand: Direct

Name: carA

Synonym: RSc1519

Alternate gene names: 17546238

Gene position: 1621137-1622273 (Clockwise)

Preceding gene: 17546237

Following gene: 17546239

Centisome position: 43.62

GC content: 65.88

Gene sequence:

>1137_bases
GTGTTGCCGTCTTTCCCGCCCGCCATTCTCGCGCTTGCAGACGGCACGGTCTTTCGTGGCTATTCCATCGGTGCCGCCGG
TCATACGATCGGCGAAGTGGTGTTCAACACCGCCATCACCGGCTACCAGGAAATCCTCACCGATCCGAGCTATTCGCGCC
AGATCGTCACGCTCACGTACCCGCATATCGGCAACGTCGGCGTGAACCGTGAGGACGTCGAAGCCACGAAAGTCCATGCC
GCCGGCCTCATCATCAAGGACCTGCCGATCCTGGCGTCGAACTTCCGCCAGGAACACTCGCTGTCGCACTACCTGAAGGG
CGAGAAGGTCGTCGCCATCGCCGGCATCGACACTCGCAAGCTCACCCGCATCCTGCGCGAGAAGGGCGCCCAGAACGGCT
GCGTGCTGGCCGGCGAAGACAACCCGCAGAAGGCCATCGACCTGGCCCGTTCCTTCCCGGGGCTGTCGGGCATGGACCTT
GCCAAGGTGGTGTCCGTCACCCAGCCGTACGAATGGAACCAGACCGAATGGGCGCTGGGCCGCGGCTACGGCGTGCAGGA
CAAGCCGCAGTTCCACGTGGTCGCCTATGACTTCGGCGTCAAGTACAACATCCTGCGCATGCTGGCCGAGCGCGGCTGCC
GCGTGACGGTGGTGCCGGCGCAGACCAGCGCCGCCGACGCGCTCGCCTACAACCCGGACGGCGTGTTCCTGTCCAACGGC
CCCGGCGATCCGCAGCCGTGCGACTACGCCATCGCCGCCACCAAGGATTTCATCGAACGCCGCATCCCGACCTTCGGCAT
CTGCCTGGGCCACCAGATCATGGGCCTGGCCGTCGGCGGCAAGACGCTGAAGATGAAGACCGGCCACCACGGCGCCAACC
ATCCGGTCAAGGACCTGCAGGACGGCCGTGTGATCATCACCTCGCAGAACCACGGCTTCGCGGTCGATCCGGAATCGCTG
CCGGCCAACGCGCGCGTGACGCACGTCTCGCTGTTCGACGGCACGCTGCAGGGCTTCGAGCTGACCGACCGTCCGGCGTT
CTGCTTCCAGGGCCACCCGGAAGCCTCGCCCGGCCCGCATGACATCGGCTATCTGTTCGACCGCTTCACCGCGGCGATGG
CCGAGCGCAAGCAGTAA

Upstream 100 bases:

>100_bases
GCCACGACGCGCAAACGGTGTGGATTCCGCCTTGCCGGATTCCGCCCGGCGGCCCGGCGTCTGACAGGTCGGCACAGGGT
GAACCCAGCAGGAGCTACCC

Downstream 100 bases:

>100_bases
CGGCAAACGAGAGCGCATAGCCATGAACGCCTTCATGTTCGCCCACTTCGGCATCATCGAGTTCTGGACCTTCCTGCTCG
GCACGATCTTCATCGTGCTG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MLPSFPPAILALADGTVFRGYSIGAAGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYPHIGNVGVNREDVEATKVHA
AGLIIKDLPILASNFRQEHSLSHYLKGEKVVAIAGIDTRKLTRILREKGAQNGCVLAGEDNPQKAIDLARSFPGLSGMDL
AKVVSVTQPYEWNQTEWALGRGYGVQDKPQFHVVAYDFGVKYNILRMLAERGCRVTVVPAQTSAADALAYNPDGVFLSNG
PGDPQPCDYAIAATKDFIERRIPTFGICLGHQIMGLAVGGKTLKMKTGHHGANHPVKDLQDGRVIITSQNHGFAVDPESL
PANARVTHVSLFDGTLQGFELTDRPAFCFQGHPEASPGPHDIGYLFDRFTAAMAERKQ

Sequences:

>Translated_378_residues
MLPSFPPAILALADGTVFRGYSIGAAGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYPHIGNVGVNREDVEATKVHA
AGLIIKDLPILASNFRQEHSLSHYLKGEKVVAIAGIDTRKLTRILREKGAQNGCVLAGEDNPQKAIDLARSFPGLSGMDL
AKVVSVTQPYEWNQTEWALGRGYGVQDKPQFHVVAYDFGVKYNILRMLAERGCRVTVVPAQTSAADALAYNPDGVFLSNG
PGDPQPCDYAIAATKDFIERRIPTFGICLGHQIMGLAVGGKTLKMKTGHHGANHPVKDLQDGRVIITSQNHGFAVDPESL
PANARVTHVSLFDGTLQGFELTDRPAFCFQGHPEASPGPHDIGYLFDRFTAAMAERKQ
>Mature_378_residues
MLPSFPPAILALADGTVFRGYSIGAAGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYPHIGNVGVNREDVEATKVHA
AGLIIKDLPILASNFRQEHSLSHYLKGEKVVAIAGIDTRKLTRILREKGAQNGCVLAGEDNPQKAIDLARSFPGLSGMDL
AKVVSVTQPYEWNQTEWALGRGYGVQDKPQFHVVAYDFGVKYNILRMLAERGCRVTVVPAQTSAADALAYNPDGVFLSNG
PGDPQPCDYAIAATKDFIERRIPTFGICLGHQIMGLAVGGKTLKMKTGHHGANHPVKDLQDGRVIITSQNHGFAVDPESL
PANARVTHVSLFDGTLQGFELTDRPAFCFQGHPEASPGPHDIGYLFDRFTAAMAERKQ

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=381, Percent_Identity=38.0577427821522, Blast_Score=234, Evalue=7e-62,
Organism=Homo sapiens, GI21361331, Length=381, Percent_Identity=35.1706036745407, Blast_Score=208, Evalue=8e-54,
Organism=Homo sapiens, GI169790915, Length=381, Percent_Identity=35.1706036745407, Blast_Score=208, Evalue=8e-54,
Organism=Escherichia coli, GI1786215, Length=376, Percent_Identity=65.9574468085106, Blast_Score=518, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI193204318, Length=383, Percent_Identity=38.1201044386423, Blast_Score=242, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6322331, Length=395, Percent_Identity=36.9620253164557, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6324878, Length=377, Percent_Identity=36.8700265251989, Blast_Score=232, Evalue=6e-62,
Organism=Drosophila melanogaster, GI45555749, Length=386, Percent_Identity=38.860103626943, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24642586, Length=386, Percent_Identity=38.860103626943, Blast_Score=225, Evalue=3e-59,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_RALSO (Q8XZ85)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519640.1
- ProteinModelPortal:   Q8XZ85
- SMR:   Q8XZ85
- GeneID:   1220348
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1519
- NMPDR:   fig|267608.1.peg.1519
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- ProtClustDB:   PRK12564
- BioCyc:   RSOL267608:RSC1519-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 40946; Mature: 40946

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 268-268 ACT_SITE 352-352 ACT_SITE 354-354

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSFPPAILALADGTVFRGYSIGAAGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTY
CCCCCCCCEEEEECCCEEECEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
PHIGNVGVNREDVEATKVHAAGLIIKDLPILASNFRQEHSLSHYLKGEKVVAIAGIDTRK
CCCCCCCCCCCCCHHHHHEECCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHH
LTRILREKGAQNGCVLAGEDNPQKAIDLARSFPGLSGMDLAKVVSVTQPYEWNQTEWALG
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCEEEC
RGYGVQDKPQFHVVAYDFGVKYNILRMLAERGCRVTVVPAQTSAADALAYNPDGVFLSNG
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCEEEECCC
PGDPQPCDYAIAATKDFIERRIPTFGICLGHQIMGLAVGGKTLKMKTGHHGANHPVKDLQ
CCCCCCCCEEHHHHHHHHHHCCCEEEEEHHCCEEEEEECCEEEEEECCCCCCCCCHHHCC
DGRVIITSQNHGFAVDPESLPANARVTHVSLFDGTLQGFELTDRPAFCFQGHPEASPGPH
CCEEEEEECCCCEEECCCCCCCCCEEEEEEEECCCCCCCEECCCCCEEECCCCCCCCCCC
DIGYLFDRFTAAMAERKQ
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLPSFPPAILALADGTVFRGYSIGAAGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTY
CCCCCCCCEEEEECCCEEECEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
PHIGNVGVNREDVEATKVHAAGLIIKDLPILASNFRQEHSLSHYLKGEKVVAIAGIDTRK
CCCCCCCCCCCCCHHHHHEECCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHH
LTRILREKGAQNGCVLAGEDNPQKAIDLARSFPGLSGMDLAKVVSVTQPYEWNQTEWALG
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCEEEC
RGYGVQDKPQFHVVAYDFGVKYNILRMLAERGCRVTVVPAQTSAADALAYNPDGVFLSNG
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCEEEECCC
PGDPQPCDYAIAATKDFIERRIPTFGICLGHQIMGLAVGGKTLKMKTGHHGANHPVKDLQ
CCCCCCCCEEHHHHHHHHHHCCCEEEEEHHCCEEEEEECCEEEEEECCCCCCCCCHHHCC
DGRVIITSQNHGFAVDPESLPANARVTHVSLFDGTLQGFELTDRPAFCFQGHPEASPGPH
CCEEEEEECCCCEEECCCCCCCCCEEEEEEEECCCCCCCEECCCCCEEECCCCCCCCCCC
DIGYLFDRFTAAMAERKQ
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852