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The map label for this gene is prpE [H]

Identifier: 17546237

GI number: 17546237

Start: 1618904

End: 1620802

Strand: Direct

Name: prpE [H]

Synonym: RSc1518

Alternate gene names: 17546237

Gene position: 1618904-1620802 (Clockwise)

Preceding gene: 17546236

Following gene: 17546238

Centisome position: 43.56

GC content: 70.72

Gene sequence:

>1899_bases
ATGCCCATGTCCGACGCCTATCGCGCGCTGTACCAGCGTTCCATCGACGATCCCGCCGCCTTCTGGGGCGAGCAGGCGCA
GCGCATCGACTGGCAGACGCCCTACGCCGCCGTGCTCGACGATGCGCGGCTGCCGTTCGCGCGCTGGTTCGTCGGCGGGC
GCACCAACCTGTGCCACAACGCCGTCGACCGCCATCTCGCCACGCGCGGCGAGCAGGCCGCGCTGGTGTATGTCTCCACC
GAGACCGGCATCGAGACGACCTACACGTACCGAGCGCTGCATCGCGAGGTCAACCGCATGGCGGCGTGCCTGCAAGCGCT
GGGCGTCAGGCGCGGCGATCGCGTGCTGATCTACCTCCCGATGATCCCGGAAGCGGCGTTCGCCATGCTGGCCTGCGCGC
GCATCGGCGCGATCCATTCGGTGGTGTTCGGCGGCTTCGCCTCCAACAGCCTCGCCACCCGCATCGACGACGCCACTCCG
CGCGTCATCGTCAGCGCCGACGCCGGCTCGCGCGGCGGCAAGGTGGTCGAATACAAGCCGCTGCTCGATGCCGCCATCGA
CCTCGCCGTGCACAAGCCGGCGCACGTCCTGCTGGTCGACCGCAAACTTGCCCCGATGCAGCACCGGCCGCACGACATTG
ACTACGCCGCGCTGGCCCGGCAGCACACCCACGCCGACGTGCCGTGCGAATGGATGGAGTCGAGCGAGCCGTCCTACATC
CTCTACACCTCGGGCACCACCGGCAAGCCCAAGGGCGTGCAGCGCGACACCGGCGGCTACGCGGTGGCGCTGGCCGCGTC
GATGCCGCTGATCTTCGGCGCGCAGGCGGGCGACACCATGTTCACCGCGTCGGACGTCGGCTGGGTGGTCGGCCACAGCT
ACATCGTCTACGCGCCGCTGCTGGCGGGGCTTGCCACCGTGATGTACGAGGGCACGCCGGTCCGCCCCGACGGCGCCATC
TGGTGGCGCATCGTCGAGCAATACCGCGTCAACGTGATGTTCACCGCGCCCACGGCCATCCGCGTGCTGAAGAAGCAGGA
TCCGGCGCTGCTGCGGCGGCATGACCTGTCCAGCCTGCGGCGCCTGTTCCTGGCCGGCGAGCCGCTCGACGAGCCCACCG
CTCGCTGGATCGGCGACGCGCTCGGCAAGCCCATCATCGACAACTACTGGCAGACCGAGACCGGCTGGCCGATGCTGGCG
ATCCCGCAGGGCGTGGCGCCCTCGACGCCCAAGCTGGGCTCGCCCGGCTTCCCGGTCTACGGATACCGGCTCGACATCCT
CGACGAGGCGACGGGCCAGCCCTGCGCGCCGGGCGAAAAGGGCCTGCTGGCCGTCGCCGCGCCGCTGCCGCCGGGCTGCA
TGAGCACCGTGTGGGGCGACGATGCACGCTTCCTCAAGACGTACTGGTCCGCCTTCCCCGGGCGCCCGCTCTATTCCAGC
TTCGACTGGGGCGTGCGCGATGAAGCGGGCTACATCACCATCCTCGGCCGCACCGATGACGTGATCAACGTGGCCGGCCA
TCGCCTGGGCACGCGCGAGATCGAAGAGAGCCTGTCGTCGCATCCGGCGATCGCCGAGGTGGCGGTGGTGGGGGTGGCCG
ACCCGCTGAAGGGGCAGGTGGCGATGGGGTTTGCCATCGTGCGCGATGCGGCCCGCGTTGCCGAGCCGGCTGGCCGCATG
GCGCTGGAGGGCGAACTGATGCGCACGGTGGAGGGGCAGTTGGGCGCCGTGGCGCGGCCGTCGCGCGTGTTCTTCGTCAA
CGCGCTGCCGAAGACGCGCTCGGGCAAGCTGCTGCGCCGGGCCATGCAGGCGGTGGCCGAGGGGCGCGATCCCGGCGACC
TGACTACCATCGAGGACCCGACCGCGCTTGCCCAGGTGCGCGAGGCGATGCAGGCGTGA

Upstream 100 bases:

>100_bases
GTCAGTCCTGTGTAAGCCGGCGCCCGCACAGTAGGCGCACATCGCACACAGAAGCAGAGCCCGCGCCGCCGCCGCCGCGG
GCCGGCCAGGAGACAACGCC

Downstream 100 bases:

>100_bases
GTCTTCCCGGAGCGGGGTGTCGGCGGCGATCCGCGGGCGAGCCGACAAGGGGCCGCCGGCAGTCGGAACCCGCAGGGTTT
TGCCGTTAAGTCCTTCCTTT

Product: propionyl-CoA synthetase

Products: NA

Alternate protein names: Propionyl-CoA synthetase [H]

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MPMSDAYRALYQRSIDDPAAFWGEQAQRIDWQTPYAAVLDDARLPFARWFVGGRTNLCHNAVDRHLATRGEQAALVYVST
ETGIETTYTYRALHREVNRMAACLQALGVRRGDRVLIYLPMIPEAAFAMLACARIGAIHSVVFGGFASNSLATRIDDATP
RVIVSADAGSRGGKVVEYKPLLDAAIDLAVHKPAHVLLVDRKLAPMQHRPHDIDYAALARQHTHADVPCEWMESSEPSYI
LYTSGTTGKPKGVQRDTGGYAVALAASMPLIFGAQAGDTMFTASDVGWVVGHSYIVYAPLLAGLATVMYEGTPVRPDGAI
WWRIVEQYRVNVMFTAPTAIRVLKKQDPALLRRHDLSSLRRLFLAGEPLDEPTARWIGDALGKPIIDNYWQTETGWPMLA
IPQGVAPSTPKLGSPGFPVYGYRLDILDEATGQPCAPGEKGLLAVAAPLPPGCMSTVWGDDARFLKTYWSAFPGRPLYSS
FDWGVRDEAGYITILGRTDDVINVAGHRLGTREIEESLSSHPAIAEVAVVGVADPLKGQVAMGFAIVRDAARVAEPAGRM
ALEGELMRTVEGQLGAVARPSRVFFVNALPKTRSGKLLRRAMQAVAEGRDPGDLTTIEDPTALAQVREAMQA

Sequences:

>Translated_632_residues
MPMSDAYRALYQRSIDDPAAFWGEQAQRIDWQTPYAAVLDDARLPFARWFVGGRTNLCHNAVDRHLATRGEQAALVYVST
ETGIETTYTYRALHREVNRMAACLQALGVRRGDRVLIYLPMIPEAAFAMLACARIGAIHSVVFGGFASNSLATRIDDATP
RVIVSADAGSRGGKVVEYKPLLDAAIDLAVHKPAHVLLVDRKLAPMQHRPHDIDYAALARQHTHADVPCEWMESSEPSYI
LYTSGTTGKPKGVQRDTGGYAVALAASMPLIFGAQAGDTMFTASDVGWVVGHSYIVYAPLLAGLATVMYEGTPVRPDGAI
WWRIVEQYRVNVMFTAPTAIRVLKKQDPALLRRHDLSSLRRLFLAGEPLDEPTARWIGDALGKPIIDNYWQTETGWPMLA
IPQGVAPSTPKLGSPGFPVYGYRLDILDEATGQPCAPGEKGLLAVAAPLPPGCMSTVWGDDARFLKTYWSAFPGRPLYSS
FDWGVRDEAGYITILGRTDDVINVAGHRLGTREIEESLSSHPAIAEVAVVGVADPLKGQVAMGFAIVRDAARVAEPAGRM
ALEGELMRTVEGQLGAVARPSRVFFVNALPKTRSGKLLRRAMQAVAEGRDPGDLTTIEDPTALAQVREAMQA
>Mature_631_residues
PMSDAYRALYQRSIDDPAAFWGEQAQRIDWQTPYAAVLDDARLPFARWFVGGRTNLCHNAVDRHLATRGEQAALVYVSTE
TGIETTYTYRALHREVNRMAACLQALGVRRGDRVLIYLPMIPEAAFAMLACARIGAIHSVVFGGFASNSLATRIDDATPR
VIVSADAGSRGGKVVEYKPLLDAAIDLAVHKPAHVLLVDRKLAPMQHRPHDIDYAALARQHTHADVPCEWMESSEPSYIL
YTSGTTGKPKGVQRDTGGYAVALAASMPLIFGAQAGDTMFTASDVGWVVGHSYIVYAPLLAGLATVMYEGTPVRPDGAIW
WRIVEQYRVNVMFTAPTAIRVLKKQDPALLRRHDLSSLRRLFLAGEPLDEPTARWIGDALGKPIIDNYWQTETGWPMLAI
PQGVAPSTPKLGSPGFPVYGYRLDILDEATGQPCAPGEKGLLAVAAPLPPGCMSTVWGDDARFLKTYWSAFPGRPLYSSF
DWGVRDEAGYITILGRTDDVINVAGHRLGTREIEESLSSHPAIAEVAVVGVADPLKGQVAMGFAIVRDAARVAEPAGRMA
LEGELMRTVEGQLGAVARPSRVFFVNALPKTRSGKLLRRAMQAVAEGRDPGDLTTIEDPTALAQVREAMQA

Specific function: Catalyzes the synthesis of propionyl-CoA from propionate and CoA. Also converts acetate to acetyl-CoA but with a lower specific activity [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=637, Percent_Identity=43.6420722135008, Blast_Score=518, Evalue=1e-147,
Organism=Homo sapiens, GI28416953, Length=640, Percent_Identity=38.59375, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI8923896, Length=664, Percent_Identity=36.4457831325301, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI253970489, Length=677, Percent_Identity=35.7459379615953, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI38505220, Length=611, Percent_Identity=26.8412438625205, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI31982927, Length=648, Percent_Identity=25.3086419753086, Blast_Score=169, Evalue=1e-41,
Organism=Homo sapiens, GI115511026, Length=560, Percent_Identity=26.9642857142857, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI42544132, Length=572, Percent_Identity=25, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI122937307, Length=537, Percent_Identity=27.7467411545624, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI58082049, Length=607, Percent_Identity=26.3591433278418, Blast_Score=162, Evalue=1e-39,
Organism=Homo sapiens, GI157311624, Length=609, Percent_Identity=26.1083743842365, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI157311622, Length=609, Percent_Identity=26.1083743842365, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI42544134, Length=362, Percent_Identity=22.0994475138122, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI187761345, Length=401, Percent_Identity=25.9351620947631, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI187761343, Length=401, Percent_Identity=25.9351620947631, Blast_Score=80, Evalue=7e-15,
Organism=Escherichia coli, GI1786529, Length=627, Percent_Identity=63.1578947368421, Blast_Score=812, Evalue=0.0,
Organism=Escherichia coli, GI1790505, Length=636, Percent_Identity=39.3081761006289, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI221142682, Length=544, Percent_Identity=23.5294117647059, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI145693145, Length=554, Percent_Identity=24.9097472924188, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1788107, Length=539, Percent_Identity=25.6029684601113, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1786810, Length=522, Percent_Identity=21.264367816092, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786801, Length=447, Percent_Identity=23.7136465324385, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71983001, Length=645, Percent_Identity=36.8992248062016, Blast_Score=387, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71982997, Length=645, Percent_Identity=36.8992248062016, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71985884, Length=599, Percent_Identity=22.8714524207012, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=563, Percent_Identity=20.4262877442274, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=535, Percent_Identity=22.2429906542056, Blast_Score=86, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6319264, Length=646, Percent_Identity=38.6996904024768, Blast_Score=457, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6323182, Length=646, Percent_Identity=37.6160990712074, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6319699, Length=376, Percent_Identity=25.531914893617, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21355225, Length=640, Percent_Identity=40.15625, Blast_Score=474, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24667955, Length=649, Percent_Identity=36.979969183359, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI62472339, Length=556, Percent_Identity=38.4892086330935, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24667959, Length=526, Percent_Identity=37.4524714828897, Blast_Score=335, Evalue=6e-92,
Organism=Drosophila melanogaster, GI21355181, Length=551, Percent_Identity=24.5009074410163, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19922652, Length=529, Percent_Identity=24.3856332703214, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648260, Length=385, Percent_Identity=27.7922077922078, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24656500, Length=519, Percent_Identity=22.9287090558767, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI18859661, Length=531, Percent_Identity=23.3521657250471, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI20130357, Length=376, Percent_Identity=25, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR012694 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.17 [H]

Molecular weight: Translated: 68738; Mature: 68607

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMSDAYRALYQRSIDDPAAFWGEQAQRIDWQTPYAAVLDDARLPFARWFVGGRTNLCHN
CCCCHHHHHHHHHCCCCHHHHHCCHHHCCCCCCCHHHHHCCCCCCHHHHEECCCCHHHHH
AVDRHLATRGEQAALVYVSTETGIETTYTYRALHREVNRMAACLQALGVRRGDRVLIYLP
HHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
MIPEAAFAMLACARIGAIHSVVFGGFASNSLATRIDDATPRVIVSADAGSRGGKVVEYKP
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEEECCCCCCCEEEEECH
LLDAAIDLAVHKPAHVLLVDRKLAPMQHRPHDIDYAALARQHTHADVPCEWMESSEPSYI
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCEE
LYTSGTTGKPKGVQRDTGGYAVALAASMPLIFGAQAGDTMFTASDVGWVVGHSYIVYAPL
EEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCEEEECCCCHHHHCHHHHHHHH
LAGLATVMYEGTPVRPDGAIWWRIVEQYRVNVMFTAPTAIRVLKKQDPALLRRHDLSSLR
HHHHHHHHCCCCCCCCCCHHHHHHHHHHEEEEEEECCHHHHHHHCCCHHHHHHHHHHHHH
RLFLAGEPLDEPTARWIGDALGKPIIDNYWQTETGWPMLAIPQGVAPSTPKLGSPGFPVY
HHHHCCCCCCCHHHHHHHHHHCCCHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEE
GYRLDILDEATGQPCAPGEKGLLAVAAPLPPGCMSTVWGDDARFLKTYWSAFPGRPLYSS
EEEEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCCC
FDWGVRDEAGYITILGRTDDVINVAGHRLGTREIEESLSSHPAIAEVAVVGVADPLKGQV
CCCCCCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHCCCCEEHEEEEECCCCCCCHH
AMGFAIVRDAARVAEPAGRMALEGELMRTVEGQLGAVARPSRVFFVNALPKTRSGKLLRR
HHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHH
AMQAVAEGRDPGDLTTIEDPTALAQVREAMQA
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PMSDAYRALYQRSIDDPAAFWGEQAQRIDWQTPYAAVLDDARLPFARWFVGGRTNLCHN
CCCHHHHHHHHHCCCCHHHHHCCHHHCCCCCCCHHHHHCCCCCCHHHHEECCCCHHHHH
AVDRHLATRGEQAALVYVSTETGIETTYTYRALHREVNRMAACLQALGVRRGDRVLIYLP
HHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
MIPEAAFAMLACARIGAIHSVVFGGFASNSLATRIDDATPRVIVSADAGSRGGKVVEYKP
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEEECCCCCCCEEEEECH
LLDAAIDLAVHKPAHVLLVDRKLAPMQHRPHDIDYAALARQHTHADVPCEWMESSEPSYI
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCEE
LYTSGTTGKPKGVQRDTGGYAVALAASMPLIFGAQAGDTMFTASDVGWVVGHSYIVYAPL
EEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCEEEECCCCHHHHCHHHHHHHH
LAGLATVMYEGTPVRPDGAIWWRIVEQYRVNVMFTAPTAIRVLKKQDPALLRRHDLSSLR
HHHHHHHHCCCCCCCCCCHHHHHHHHHHEEEEEEECCHHHHHHHCCCHHHHHHHHHHHHH
RLFLAGEPLDEPTARWIGDALGKPIIDNYWQTETGWPMLAIPQGVAPSTPKLGSPGFPVY
HHHHCCCCCCCHHHHHHHHHHCCCHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEE
GYRLDILDEATGQPCAPGEKGLLAVAAPLPPGCMSTVWGDDARFLKTYWSAFPGRPLYSS
EEEEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCCC
FDWGVRDEAGYITILGRTDDVINVAGHRLGTREIEESLSSHPAIAEVAVVGVADPLKGQV
CCCCCCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHCCCCEEHEEEEECCCCCCCHH
AMGFAIVRDAARVAEPAGRMALEGELMRTVEGQLGAVARPSRVFFVNALPKTRSGKLLRR
HHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHH
AMQAVAEGRDPGDLTTIEDPTALAQVREAMQA
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9006051; 10411265; 11677609; 10482501 [H]