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The map label for this gene is gloB [H]

Identifier: 17546234

GI number: 17546234

Start: 1616227

End: 1617081

Strand: Direct

Name: gloB [H]

Synonym: RSc1515

Alternate gene names: 17546234

Gene position: 1616227-1617081 (Clockwise)

Preceding gene: 17546230

Following gene: 17546235

Centisome position: 43.49

GC content: 69.36

Gene sequence:

>855_bases
ATGCGACGATCGGAAGAGGAGTCGGGCATGGTCCAGGGTGGTGTGCCGCTTGCCGTGGAGGCGATCGCTGCGTTTACCGA
CAATTATATTTGGGCACTTCACGATGGCCGCGTGGCCGCCGTGGTCGATCCCGGCGACGCGCAACCGGTGCTCCGCTTCC
TGCAGGCGCGCGGTCTGGCACTCGGTGCCATTGTAATCACACATCATCACGGCGATCACGTCGGCGGCGTGCAGGCGCTG
GTCGAGGCCTATCCGCGCGACCCGTCCGGCGCGCCGTTGCCGGTGATCGGCCCGGCGGACGAGTCGATTCCCTGCCGCAC
GCAGGCTGTGCGCGAGGGCGACACCGTGACCCTCTCGCATCCCGCCGCCACCTTCCACGTGCTCGATGTGCCCGGCCATA
CGCGTGGCCATGTCGCCTATGTCGGCCGCCTGCAGGGGCCGGATGCGCCCGCCAGCCTGTTCTGCGGCGATACGCTGTTC
GCCACCGGCTGCGGCCGCCTGTTCGAGGGCACGCCCGCGCAGATGCGGCTGTCGCTGTCCAAGCTGGCCGCGCTGCCGCC
CGACACGCGCGTGTACTGCGCACACGAATACACCGCCGCGAATGTGCGTTTCGCGCGCGCGGTCGAACCGGGCAACGCCG
CGCTGGCGGCATGGGAAGACGAAGTCGCCTCGCTGCGCACCGACGGGCGCGCGACCGTGCCGACCACGGTCGGCCACGAG
CGCGCGACCAATCCCTTCATGCGATCGAACGAGCCCGCCGTGGCGGCCTCGGTGGCGCAGCATGCCGGTATCGGAGTCGA
CGATCCGGTGGCCGTTTTCGCGGCGCTGCGCGAATGGAAAAACGGATTTCGCTGA

Upstream 100 bases:

>100_bases
GAAGCCGAACATGTCGGCGACGGTGCGGTCGAATTGGGCCTGTTCCCAACGCAGGACGTATTGCCCGGGCGGCGAAGCAA
GCCAGTGATGCCAATCTATA

Downstream 100 bases:

>100_bases
GCCGGCGCGCCAAAAGGTGCGCTAGGCGACGCTTTTTCCCGGCGTGAACGGTCCCATGCCGGGTGAAAGCGGGTTGACGC
GGCGGAATGCCTTCCATACC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MRRSEEESGMVQGGVPLAVEAIAAFTDNYIWALHDGRVAAVVDPGDAQPVLRFLQARGLALGAIVITHHHGDHVGGVQAL
VEAYPRDPSGAPLPVIGPADESIPCRTQAVREGDTVTLSHPAATFHVLDVPGHTRGHVAYVGRLQGPDAPASLFCGDTLF
ATGCGRLFEGTPAQMRLSLSKLAALPPDTRVYCAHEYTAANVRFARAVEPGNAALAAWEDEVASLRTDGRATVPTTVGHE
RATNPFMRSNEPAVAASVAQHAGIGVDDPVAVFAALREWKNGFR

Sequences:

>Translated_284_residues
MRRSEEESGMVQGGVPLAVEAIAAFTDNYIWALHDGRVAAVVDPGDAQPVLRFLQARGLALGAIVITHHHGDHVGGVQAL
VEAYPRDPSGAPLPVIGPADESIPCRTQAVREGDTVTLSHPAATFHVLDVPGHTRGHVAYVGRLQGPDAPASLFCGDTLF
ATGCGRLFEGTPAQMRLSLSKLAALPPDTRVYCAHEYTAANVRFARAVEPGNAALAAWEDEVASLRTDGRATVPTTVGHE
RATNPFMRSNEPAVAASVAQHAGIGVDDPVAVFAALREWKNGFR
>Mature_284_residues
MRRSEEESGMVQGGVPLAVEAIAAFTDNYIWALHDGRVAAVVDPGDAQPVLRFLQARGLALGAIVITHHHGDHVGGVQAL
VEAYPRDPSGAPLPVIGPADESIPCRTQAVREGDTVTLSHPAATFHVLDVPGHTRGHVAYVGRLQGPDAPASLFCGDTLF
ATGCGRLFEGTPAQMRLSLSKLAALPPDTRVYCAHEYTAANVRFARAVEPGNAALAAWEDEVASLRTDGRATVPTTVGHE
RATNPFMRSNEPAVAASVAQHAGIGVDDPVAVFAALREWKNGFR

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=274, Percent_Identity=40.8759124087591, Blast_Score=178, Evalue=4e-45,
Organism=Homo sapiens, GI94538322, Length=274, Percent_Identity=40.8759124087591, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI14150041, Length=241, Percent_Identity=37.344398340249, Blast_Score=137, Evalue=8e-33,
Organism=Homo sapiens, GI116642887, Length=266, Percent_Identity=33.8345864661654, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI21703352, Length=264, Percent_Identity=34.0909090909091, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI46361987, Length=199, Percent_Identity=37.6884422110553, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1786406, Length=270, Percent_Identity=41.8518518518518, Blast_Score=189, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17536925, Length=273, Percent_Identity=35.1648351648352, Blast_Score=151, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6320478, Length=272, Percent_Identity=30.1470588235294, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324614, Length=266, Percent_Identity=31.203007518797, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24667711, Length=273, Percent_Identity=39.1941391941392, Blast_Score=185, Evalue=2e-47,
Organism=Drosophila melanogaster, GI21356335, Length=273, Percent_Identity=39.1941391941392, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24667703, Length=273, Percent_Identity=39.1941391941392, Blast_Score=184, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 29970; Mature: 29970

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSEEESGMVQGGVPLAVEAIAAFTDNYIWALHDGRVAAVVDPGDAQPVLRFLQARGLA
CCCCCCCCCEEECCCCHHHHHHHHHCCCEEEEEECCEEEEEECCCCHHHHHHHHHHCCCE
LGAIVITHHHGDHVGGVQALVEAYPRDPSGAPLPVIGPADESIPCRTQAVREGDTVTLSH
EEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHCCCCEEEEEC
PAATFHVLDVPGHTRGHVAYVGRLQGPDAPASLFCGDTLFATGCGRLFEGTPAQMRLSLS
CCEEEEEEECCCCCCCEEEEEEEECCCCCCCEEEECCHHHHHCCCHHHCCCCHHHHHHHH
KLAALPPDTRVYCAHEYTAANVRFARAVEPGNAALAAWEDEVASLRTDGRATVPTTVGHE
HHHCCCCCCEEEEEECEEECCEEEEEEECCCCCEEEEHHHHHHHHHCCCCEECCCCCCCC
RATNPFMRSNEPAVAASVAQHAGIGVDDPVAVFAALREWKNGFR
CCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRRSEEESGMVQGGVPLAVEAIAAFTDNYIWALHDGRVAAVVDPGDAQPVLRFLQARGLA
CCCCCCCCCEEECCCCHHHHHHHHHCCCEEEEEECCEEEEEECCCCHHHHHHHHHHCCCE
LGAIVITHHHGDHVGGVQALVEAYPRDPSGAPLPVIGPADESIPCRTQAVREGDTVTLSH
EEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHCCCCEEEEEC
PAATFHVLDVPGHTRGHVAYVGRLQGPDAPASLFCGDTLFATGCGRLFEGTPAQMRLSLS
CCEEEEEEECCCCCCCEEEEEEEECCCCCCCEEEECCHHHHHCCCHHHCCCCHHHHHHHH
KLAALPPDTRVYCAHEYTAANVRFARAVEPGNAALAAWEDEVASLRTDGRATVPTTVGHE
HHHCCCCCCEEEEEECEEECCEEEEEEECCCCCEEEEHHHHHHHHHCCCCEECCCCCCCC
RATNPFMRSNEPAVAASVAQHAGIGVDDPVAVFAALREWKNGFR
CCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA