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The map label for this gene is rnhA

Identifier: 17546232

GI number: 17546232

Start: 1614866

End: 1615321

Strand: Reverse

Name: rnhA

Synonym: RSc1513

Alternate gene names: 17546232

Gene position: 1615321-1614866 (Counterclockwise)

Preceding gene: 17546233

Following gene: 17546231

Centisome position: 43.46

GC content: 65.35

Gene sequence:

>456_bases
ATGCAGGAAGTCACCGTCTATTCCGATGGCGCATGCAAGGGCAACCCGGGCCTCGGCGGCTGGGGCACCGTGCTGGTCTC
GGGCGGCCACGAAAAAGAGCTGTTCGGCGGCGAGGCCGTCACCACAAACAACCGCATGGAGCTGATGGCCGTCATCGAAG
CCTTCCGCGCGCTCAAGCGTCCGTGCCGGGTCAAGGTCTATACCGATTCGCAATACGTGCAGAAAGGCATCAGCGAATGG
CTGGCGGGCTGGAAAGCACGCGGCTGGAAGACCGCCGACAAGAAGCCCGTGAAGAACGACGACCTGTGGCGCACGCTCGA
TGAACTGGTGGTCACGCACGAGGTGAGCTGGCACTGGGTCAAGGGCCACGCGGGCCACCCCGGCAACGAGCGCGCCGATG
CGCTGGCCAACAAGGGGGTGGAGATAGCGCGCCAGGCCATTCAGGCCGGCGCCTGA

Upstream 100 bases:

>100_bases
TCGCACACCACCAAGACACCGCCCGTCGCCGCGCGCGACGGCAAGTCCGGCGCCTGAGCCGGGCCCGCGGTATCCCCCTC
ACCTATCACCGATCGTTTTC

Downstream 100 bases:

>100_bases
CACGCGACTGCAGTCGCCCCTCGGTCACCGCCCGAGGGATCCCGCGATCACCGGCCGTGCCCACACGGCGGCGGCGCTTC
GTTCTCGGACAGCCTCGCGC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MQEVTVYSDGACKGNPGLGGWGTVLVSGGHEKELFGGEAVTTNNRMELMAVIEAFRALKRPCRVKVYTDSQYVQKGISEW
LAGWKARGWKTADKKPVKNDDLWRTLDELVVTHEVSWHWVKGHAGHPGNERADALANKGVEIARQAIQAGA

Sequences:

>Translated_151_residues
MQEVTVYSDGACKGNPGLGGWGTVLVSGGHEKELFGGEAVTTNNRMELMAVIEAFRALKRPCRVKVYTDSQYVQKGISEW
LAGWKARGWKTADKKPVKNDDLWRTLDELVVTHEVSWHWVKGHAGHPGNERADALANKGVEIARQAIQAGA
>Mature_151_residues
MQEVTVYSDGACKGNPGLGGWGTVLVSGGHEKELFGGEAVTTNNRMELMAVIEAFRALKRPCRVKVYTDSQYVQKGISEW
LAGWKARGWKTADKKPVKNDDLWRTLDELVVTHEVSWHWVKGHAGHPGNERADALANKGVEIARQAIQAGA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=143, Percent_Identity=34.965034965035, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1786408, Length=136, Percent_Identity=58.0882352941177, Blast_Score=171, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI115532542, Length=142, Percent_Identity=38.0281690140845, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137476, Length=152, Percent_Identity=35.5263157894737, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45552509, Length=152, Percent_Identity=35.5263157894737, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_RALSO (Q8XZ91)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519634.1
- ProteinModelPortal:   Q8XZ91
- SMR:   Q8XZ91
- GeneID:   1220342
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1513
- NMPDR:   fig|267608.1.peg.1513
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- ProtClustDB:   PRK00203
- BioCyc:   RSOL267608:RSC1513-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 16598; Mature: 16598

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEVTVYSDGACKGNPGLGGWGTVLVSGGHEKELFGGEAVTTNNRMELMAVIEAFRALKR
CCCEEEEECCCCCCCCCCCCCEEEEECCCCCCHHCCCCEEECCCHHHHHHHHHHHHHHCC
PCRVKVYTDSQYVQKGISEWLAGWKARGWKTADKKPVKNDDLWRTLDELVVTHEVSWHWV
CCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEE
KGHAGHPGNERADALANKGVEIARQAIQAGA
ECCCCCCCCHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MQEVTVYSDGACKGNPGLGGWGTVLVSGGHEKELFGGEAVTTNNRMELMAVIEAFRALKR
CCCEEEEECCCCCCCCCCCCCEEEEECCCCCCHHCCCCEEECCCHHHHHHHHHHHHHHCC
PCRVKVYTDSQYVQKGISEWLAGWKARGWKTADKKPVKNDDLWRTLDELVVTHEVSWHWV
CCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEE
KGHAGHPGNERADALANKGVEIARQAIQAGA
ECCCCCCCCHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852