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The map label for this gene is tISRso12b [C]

Identifier: 17546158

GI number: 17546158

Start: 1542234

End: 1542938

Strand: Direct

Name: tISRso12b [C]

Synonym: RSc1439

Alternate gene names: 17546158

Gene position: 1542234-1542938 (Clockwise)

Preceding gene: 17546157

Following gene: 17546160

Centisome position: 41.5

GC content: 58.72

Gene sequence:

>705_bases
ATGCACGAGATCGCCAGCACCCGGGTACGGTACGGCTTCTGGCGCATTTACGTGCTGATTCGTCGCGAGGGTTGGTTGGT
CAATCACAAACGCGTCTACCGCCTGTACAAGGAGGAAGGCTTGAATCTGCGCACCAAAAGACCCAGGCGCCGCAAGGCGG
CCGCACATAGACTCGAGCGCCCGGTGCTCACCGCACCGAATCAGAGCTGGAGCATGGATTTCGTATCCGACGCCTTGTTT
AACGGCAACAAGTTCCGCGCACTGACTGTGGTGGACAACCATACGCGGGAATGCCTGGCGATTGAGGCTGCCCCGTCGCT
AAACGGCAGTGACGTTGTGCGTGTGTTGCAAGCCATCGTGATCCGACGAGGCATGGTGCCCGCACGCATCCAGGCTGACA
ACGGGCCGGAATTTGTGTCGCTGGCGCTGGACAAGTGGGCGTATGAGGCAGGCGTAACGCTCGACTTCTCTCGGCCTGGC
AAGCCTACCGACAATGCCTTCATCGAGTCCTTCAACGGCAGCTTGCGCGATGAATGCCTGAACACGTACTGGTTCCTGTC
CCTTGAAGACGCACGCCAAAAGCTCGAAAACTGGAGGCACGATTACAATCATTTCCGGCCACATTCGGCGCTGGATGACA
CCGCACCAGCGCTCTTTGCACGGCAGTTTGCCATTACACCAACCACGCCGGCTTCTCTAGCCTGA

Upstream 100 bases:

>100_bases
CGTCAGCATTAAACGCGCCTGCCAGGTCTGCCGGCAAAGCCGCGCAGGTTGGTACCAGAAGCCGAAACCGAGGCTGCTGG
ACGCACCGATCAAGGCCCGC

Downstream 100 bases:

>100_bases
CCCTGTCCAGTTCTTGGGAGGAGGTCAATGCCTGTTCATGTTCTTGTGGATAGTTGTACCCTTGCGAGGGGGGCTGCCGC
TTGTTTTTGCCTTGACCGTG

Product: ISRSO12-transposase ORFB protein

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MHEIASTRVRYGFWRIYVLIRREGWLVNHKRVYRLYKEEGLNLRTKRPRRRKAAAHRLERPVLTAPNQSWSMDFVSDALF
NGNKFRALTVVDNHTRECLAIEAAPSLNGSDVVRVLQAIVIRRGMVPARIQADNGPEFVSLALDKWAYEAGVTLDFSRPG
KPTDNAFIESFNGSLRDECLNTYWFLSLEDARQKLENWRHDYNHFRPHSALDDTAPALFARQFAITPTTPASLA

Sequences:

>Translated_234_residues
MHEIASTRVRYGFWRIYVLIRREGWLVNHKRVYRLYKEEGLNLRTKRPRRRKAAAHRLERPVLTAPNQSWSMDFVSDALF
NGNKFRALTVVDNHTRECLAIEAAPSLNGSDVVRVLQAIVIRRGMVPARIQADNGPEFVSLALDKWAYEAGVTLDFSRPG
KPTDNAFIESFNGSLRDECLNTYWFLSLEDARQKLENWRHDYNHFRPHSALDDTAPALFARQFAITPTTPASLA
>Mature_234_residues
MHEIASTRVRYGFWRIYVLIRREGWLVNHKRVYRLYKEEGLNLRTKRPRRRKAAAHRLERPVLTAPNQSWSMDFVSDALF
NGNKFRALTVVDNHTRECLAIEAAPSLNGSDVVRVLQAIVIRRGMVPARIQADNGPEFVSLALDKWAYEAGVTLDFSRPG
KPTDNAFIESFNGSLRDECLNTYWFLSLEDARQKLENWRHDYNHFRPHSALDDTAPALFARQFAITPTTPASLA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786462, Length=175, Percent_Identity=32, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789981, Length=228, Percent_Identity=25.8771929824561, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790724, Length=237, Percent_Identity=26.1603375527426, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789424, Length=237, Percent_Identity=26.1603375527426, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789224, Length=237, Percent_Identity=26.1603375527426, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788305, Length=237, Percent_Identity=26.1603375527426, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787669, Length=237, Percent_Identity=26.1603375527426, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786558, Length=237, Percent_Identity=26.1603375527426, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 26955; Mature: 26955

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEIASTRVRYGFWRIYVLIRREGWLVNHKRVYRLYKEEGLNLRTKRPRRRKAAAHRLER
CCCCHHHHHHHHHEEEEEEEECCCCEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
PVLTAPNQSWSMDFVSDALFNGNKFRALTVVDNHTRECLAIEAAPSLNGSDVVRVLQAIV
CCEECCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHH
IRRGMVPARIQADNGPEFVSLALDKWAYEAGVTLDFSRPGKPTDNAFIESFNGSLRDECL
HHCCCCCCEEECCCCCCEEHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHCCCHHHHHHC
NTYWFLSLEDARQKLENWRHDYNHFRPHSALDDTAPALFARQFAITPTTPASLA
CCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEECCCCCCCCCC
>Mature Secondary Structure
MHEIASTRVRYGFWRIYVLIRREGWLVNHKRVYRLYKEEGLNLRTKRPRRRKAAAHRLER
CCCCHHHHHHHHHEEEEEEEECCCCEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
PVLTAPNQSWSMDFVSDALFNGNKFRALTVVDNHTRECLAIEAAPSLNGSDVVRVLQAIV
CCEECCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHH
IRRGMVPARIQADNGPEFVSLALDKWAYEAGVTLDFSRPGKPTDNAFIESFNGSLRDECL
HHCCCCCCEEECCCCCCEEHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHCCCHHHHHHC
NTYWFLSLEDARQKLENWRHDYNHFRPHSALDDTAPALFARQFAITPTTPASLA
CCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]