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The map label for this gene is ppsA [H]

Identifier: 17546140

GI number: 17546140

Start: 1520988

End: 1523375

Strand: Direct

Name: ppsA [H]

Synonym: RSc1421

Alternate gene names: 17546140

Gene position: 1520988-1523375 (Clockwise)

Preceding gene: 17546138

Following gene: 17546141

Centisome position: 40.93

GC content: 65.7

Gene sequence:

>2388_bases
ATGACCAACCTGTCGATGGACGGCGCCTACGTGCTGCCGTTCGAGCAATTGCGGATGACCGATGTCGAGGTCGTCGGCGG
CAAGAACGCATCGCTGGGCGAGATGATCTCCCAACTGGACAGCGCCGGCGTGCGCGTTCCGGGCGGTTTTGCCACCACGG
CACTGGCTTTCCGCGATTTCCTCCAGCACAACAACCTGACCGAGCGCATTTCCCAGCGCCTGGCCAGCCTGGACGTCGAC
GACGTCAAGGCGCTGGCCGCGGCCGGCAAGGAAATCCGCGAATGGGTCGCCACGGCGCCGTTCCAGTCGCGCCTGGAGCA
GGAGATCCGCGAGCATTTCGAACGCGTCTCCAAGCGCGAGGGCGCCGAGGCCTCGTTCGCGGTGCGCTCGTCGGCCACGG
CCGAAGACCTGCCGGACGCCTCGTTCGCCGGCCAGCAGGAGAGCTACCTCAACGTCTCCGGCATCGATGACGTGCTCGAC
AAGATCAAGCACGTGTTCGCCTCGCTGTACAACGACCGCGCTATCTCCTACCGCGTGCACAAGGGCTTCGCCCACGACGT
GGTGGCGCTCTCCGCCGGCATCCAGCGCATGGTGCGCTCGGACTGCGGCGCGTCGGGCGTGATGTTCACGATCGACACCG
AATCGGGCTTCCAGGACGTCGTCTTCATCACCTCCAGCTACGGCCTGGGCGAGACGGTGGTGCAGGGCGCGGTCAACCCG
GACGAGTTCTACGTGTTCAAGCCGACGCTGGCCGCCGGCAAGTACCCGATCATCCGCCGCTCGATCGGCTCCAAGCTGAT
CAAGATGGAGTTCACGCAGGCCGGCGAGGAAGGCCGCGTCAAGACCGTCGACGTGCCGGGCGAGTTGCGCAACCGCTACT
CGCTGGTCGACGAAGACGTGGTCGAGCTGGCCAAGTACGCCGTCATCATCGAGAAGCACTACGGTCGCCCGATGGACATC
GAGTGGGGTAAGGACGGTAAGGACGGCAAGATCTACATCCTGCAGGCCCGCCCCGAGACGGTGAAGAGCCAGTCGGTCGG
CAAGGTCGAGCAGCGCTTCCGCCTGAAGGGCTCGGCGCCGGTGCTGACCACCGGCCGCGCGATCGGCCAGAAGATCGGTA
CGGGCCCCGTGCGCGTGATCAACGATCCGGCCGAAATGGAGCGCGTGCAGCCGGGCGACGTGCTGGTCGCCGACATGACC
GACCCGAACTGGGAGCCGGTGATGAAGCGCGCCTCGGCCATCGTCACCAACCGTGGCGGCCGCACCTGTCACGCCGCCAT
CATCGCGCGTGAGCTGGGCGTGCCGGCCGTGGTCGGCTGCGGCGACGCCACCGACCTGCTGAAGGACGGCACGCTGGTCA
CCGTGTCCTGCGCCGAGGGCGACGAAGGCAAGATCTACGACGGCCTGCTCGAGACGGAAATCACCGAAGTGCGCCGCGGC
GAGATGCCGCCGATCGACGTCAAGATCATGATGAACGTCGGCAACCCGCAGCTGGCCTTCGAGTTCGCGCAGATCCCGAA
CGGCGGCGTGGGCCTGGCCCGCCTCGAGTTCATCATCAACAACAACATCGGCGTCCACCCGAAGGCGATCCTCGACTACC
CGCAAGTCGACAGCGACCTGAAGAAGGCCGTGGAAAGCGTGGCCCGCGGCCATGCCAGCCCGCGCGCGTTCTACGTCGAC
AAGCTGGCCGAGGGCATTGCGACCATCGCCGCCGCGTTCTATCCGAAGCCCGTGATCGTGCGCCTGTCCGACTTCAAGTC
GAACGAGTACAAGAAGCTGATCGGCGGTTCGCGCTACGAGCCGGACGAAGAGAACCCCATGCTGGGCTTCCGCGGCGCCT
CGCGTTACATCGCCGAAGACTTCGCCGAGGCCTTCGAGATGGAGTGCCGCGCCATGAAGCGCGTGCGCGAAGAGATGGGC
CTGACCAACGTCGAGATCATGGTGCCGTTCGTGCGCACGCTGGGCCAGGCCGCCATGGTGGTCGACCTGCTGGGCAAGTA
CGGCCTGAAGCGCGGCGAGAACGACCTGCGCCTGATCATGATGTGCGAAGTGCCGTCCAATGCGATCCTGGCCAAGGAGT
TCCTGGAGTATTTCGACGGCTTCTCGATCGGCTCGAACGACCTGACGCAGCTGACGCTGGGCCTGGACCGCGACTCCGGC
ATGGAACTGCTCGCCAGGGATTTCGACGAGCGTGATCCGGCGGTCAAGTTCATGCTGTCGCGCGCCATCTCGACCGCGAA
GTCGATGGGCAAGTATGTCGGCATCTGCGGCCAGGGCCCGTCCGACCACCCGGACTTTGCCGAGTGGCTGGCCAAGGAAG
GCATCTCGTCCATCTCGCTGAACCCGGATTCGGTGATCGACACCTGGCAGAAGCTGGGTTCGGCCTGA

Upstream 100 bases:

>100_bases
CCGCCTTCCGGATGATTTAGCTAAGCGATTCGCGCGGCCAGACGACAAGAACACAGCGCCGAGCGCATCCGATCGGTTTT
CCACTTAGTAAGAGGCTTGT

Downstream 100 bases:

>100_bases
TCCCGCTGTAAAAGCGGCGCGCGCCCCGGCGGGTGCGCGCCGACAAAAACGCGAAGTGCCCGGTGGCGTCCAACGCTGGC
GGGGGCGGAGCGATCAGCCC

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 795; Mature: 794

Protein sequence:

>795_residues
MTNLSMDGAYVLPFEQLRMTDVEVVGGKNASLGEMISQLDSAGVRVPGGFATTALAFRDFLQHNNLTERISQRLASLDVD
DVKALAAAGKEIREWVATAPFQSRLEQEIREHFERVSKREGAEASFAVRSSATAEDLPDASFAGQQESYLNVSGIDDVLD
KIKHVFASLYNDRAISYRVHKGFAHDVVALSAGIQRMVRSDCGASGVMFTIDTESGFQDVVFITSSYGLGETVVQGAVNP
DEFYVFKPTLAAGKYPIIRRSIGSKLIKMEFTQAGEEGRVKTVDVPGELRNRYSLVDEDVVELAKYAVIIEKHYGRPMDI
EWGKDGKDGKIYILQARPETVKSQSVGKVEQRFRLKGSAPVLTTGRAIGQKIGTGPVRVINDPAEMERVQPGDVLVADMT
DPNWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDLLKDGTLVTVSCAEGDEGKIYDGLLETEITEVRRG
EMPPIDVKIMMNVGNPQLAFEFAQIPNGGVGLARLEFIINNNIGVHPKAILDYPQVDSDLKKAVESVARGHASPRAFYVD
KLAEGIATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIAEDFAEAFEMECRAMKRVREEMG
LTNVEIMVPFVRTLGQAAMVVDLLGKYGLKRGENDLRLIMMCEVPSNAILAKEFLEYFDGFSIGSNDLTQLTLGLDRDSG
MELLARDFDERDPAVKFMLSRAISTAKSMGKYVGICGQGPSDHPDFAEWLAKEGISSISLNPDSVIDTWQKLGSA

Sequences:

>Translated_795_residues
MTNLSMDGAYVLPFEQLRMTDVEVVGGKNASLGEMISQLDSAGVRVPGGFATTALAFRDFLQHNNLTERISQRLASLDVD
DVKALAAAGKEIREWVATAPFQSRLEQEIREHFERVSKREGAEASFAVRSSATAEDLPDASFAGQQESYLNVSGIDDVLD
KIKHVFASLYNDRAISYRVHKGFAHDVVALSAGIQRMVRSDCGASGVMFTIDTESGFQDVVFITSSYGLGETVVQGAVNP
DEFYVFKPTLAAGKYPIIRRSIGSKLIKMEFTQAGEEGRVKTVDVPGELRNRYSLVDEDVVELAKYAVIIEKHYGRPMDI
EWGKDGKDGKIYILQARPETVKSQSVGKVEQRFRLKGSAPVLTTGRAIGQKIGTGPVRVINDPAEMERVQPGDVLVADMT
DPNWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDLLKDGTLVTVSCAEGDEGKIYDGLLETEITEVRRG
EMPPIDVKIMMNVGNPQLAFEFAQIPNGGVGLARLEFIINNNIGVHPKAILDYPQVDSDLKKAVESVARGHASPRAFYVD
KLAEGIATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIAEDFAEAFEMECRAMKRVREEMG
LTNVEIMVPFVRTLGQAAMVVDLLGKYGLKRGENDLRLIMMCEVPSNAILAKEFLEYFDGFSIGSNDLTQLTLGLDRDSG
MELLARDFDERDPAVKFMLSRAISTAKSMGKYVGICGQGPSDHPDFAEWLAKEGISSISLNPDSVIDTWQKLGSA
>Mature_794_residues
TNLSMDGAYVLPFEQLRMTDVEVVGGKNASLGEMISQLDSAGVRVPGGFATTALAFRDFLQHNNLTERISQRLASLDVDD
VKALAAAGKEIREWVATAPFQSRLEQEIREHFERVSKREGAEASFAVRSSATAEDLPDASFAGQQESYLNVSGIDDVLDK
IKHVFASLYNDRAISYRVHKGFAHDVVALSAGIQRMVRSDCGASGVMFTIDTESGFQDVVFITSSYGLGETVVQGAVNPD
EFYVFKPTLAAGKYPIIRRSIGSKLIKMEFTQAGEEGRVKTVDVPGELRNRYSLVDEDVVELAKYAVIIEKHYGRPMDIE
WGKDGKDGKIYILQARPETVKSQSVGKVEQRFRLKGSAPVLTTGRAIGQKIGTGPVRVINDPAEMERVQPGDVLVADMTD
PNWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDLLKDGTLVTVSCAEGDEGKIYDGLLETEITEVRRGE
MPPIDVKIMMNVGNPQLAFEFAQIPNGGVGLARLEFIINNNIGVHPKAILDYPQVDSDLKKAVESVARGHASPRAFYVDK
LAEGIATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIAEDFAEAFEMECRAMKRVREEMGL
TNVEIMVPFVRTLGQAAMVVDLLGKYGLKRGENDLRLIMMCEVPSNAILAKEFLEYFDGFSIGSNDLTQLTLGLDRDSGM
ELLARDFDERDPAVKFMLSRAISTAKSMGKYVGICGQGPSDHPDFAEWLAKEGISSISLNPDSVIDTWQKLGSA

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=783, Percent_Identity=64.6232439335888, Blast_Score=1041, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=411, Percent_Identity=26.0340632603406, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1788726, Length=193, Percent_Identity=33.6787564766839, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI48994992, Length=409, Percent_Identity=25.6723716381418, Blast_Score=90, Evalue=7e-19,
Organism=Escherichia coli, GI1789193, Length=406, Percent_Identity=26.3546798029557, Blast_Score=83, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17564524, Length=330, Percent_Identity=28.4848484848485, Blast_Score=93, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87052; Mature: 86921

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLSMDGAYVLPFEQLRMTDVEVVGGKNASLGEMISQLDSAGVRVPGGFATTALAFRDF
CCCCCCCCEEECCHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LQHNNLTERISQRLASLDVDDVKALAAAGKEIREWVATAPFQSRLEQEIREHFERVSKRE
HHCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
GAEASFAVRSSATAEDLPDASFAGQQESYLNVSGIDDVLDKIKHVFASLYNDRAISYRVH
CCCCEEEECCCCCHHCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCEEEEEEC
KGFAHDVVALSAGIQRMVRSDCGASGVMFTIDTESGFQDVVFITSSYGLGETVVQGAVNP
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHCCCCC
DEFYVFKPTLAAGKYPIIRRSIGSKLIKMEFTQAGEEGRVKTVDVPGELRNRYSLVDEDV
CCEEEECCCCCCCCCHHHHHHHCCHHEEEHHHHCCCCCCEEEEECCHHHHHHHHHHHHHH
VELAKYAVIIEKHYGRPMDIEWGKDGKDGKIYILQARPETVKSQSVGKVEQRFRLKGSAP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCC
VLTTGRAIGQKIGTGPVRVINDPAEMERVQPGDVLVADMTDPNWEPVMKRASAIVTNRGG
EEECCHHHHHHCCCCCEEEECCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCC
RTCHAAIIARELGVPAVVGCGDATDLLKDGTLVTVSCAEGDEGKIYDGLLETEITEVRRG
CHHHHHHHHHHCCCCEEEECCCHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHCC
EMPPIDVKIMMNVGNPQLAFEFAQIPNGGVGLARLEFIINNNIGVHPKAILDYPQVDSDL
CCCCCEEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCCHHH
KKAVESVARGHASPRAFYVDKLAEGIATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCC
PDEENPMLGFRGASRYIAEDFAEAFEMECRAMKRVREEMGLTNVEIMVPFVRTLGQAAMV
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VDLLGKYGLKRGENDLRLIMMCEVPSNAILAKEFLEYFDGFSIGSNDLTQLTLGLDRDSG
HHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
MELLARDFDERDPAVKFMLSRAISTAKSMGKYVGICGQGPSDHPDFAEWLAKEGISSISL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHCCCCEECC
NPDSVIDTWQKLGSA
CCHHHHHHHHHHCCC
>Mature Secondary Structure 
TNLSMDGAYVLPFEQLRMTDVEVVGGKNASLGEMISQLDSAGVRVPGGFATTALAFRDF
CCCCCCCEEECCHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LQHNNLTERISQRLASLDVDDVKALAAAGKEIREWVATAPFQSRLEQEIREHFERVSKRE
HHCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
GAEASFAVRSSATAEDLPDASFAGQQESYLNVSGIDDVLDKIKHVFASLYNDRAISYRVH
CCCCEEEECCCCCHHCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCEEEEEEC
KGFAHDVVALSAGIQRMVRSDCGASGVMFTIDTESGFQDVVFITSSYGLGETVVQGAVNP
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHCCCCC
DEFYVFKPTLAAGKYPIIRRSIGSKLIKMEFTQAGEEGRVKTVDVPGELRNRYSLVDEDV
CCEEEECCCCCCCCCHHHHHHHCCHHEEEHHHHCCCCCCEEEEECCHHHHHHHHHHHHHH
VELAKYAVIIEKHYGRPMDIEWGKDGKDGKIYILQARPETVKSQSVGKVEQRFRLKGSAP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCC
VLTTGRAIGQKIGTGPVRVINDPAEMERVQPGDVLVADMTDPNWEPVMKRASAIVTNRGG
EEECCHHHHHHCCCCCEEEECCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCC
RTCHAAIIARELGVPAVVGCGDATDLLKDGTLVTVSCAEGDEGKIYDGLLETEITEVRRG
CHHHHHHHHHHCCCCEEEECCCHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHCC
EMPPIDVKIMMNVGNPQLAFEFAQIPNGGVGLARLEFIINNNIGVHPKAILDYPQVDSDL
CCCCCEEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCCHHH
KKAVESVARGHASPRAFYVDKLAEGIATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSRYE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCC
PDEENPMLGFRGASRYIAEDFAEAFEMECRAMKRVREEMGLTNVEIMVPFVRTLGQAAMV
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VDLLGKYGLKRGENDLRLIMMCEVPSNAILAKEFLEYFDGFSIGSNDLTQLTLGLDRDSG
HHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
MELLARDFDERDPAVKFMLSRAISTAKSMGKYVGICGQGPSDHPDFAEWLAKEGISSISL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHCCCCEECC
NPDSVIDTWQKLGSA
CCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]