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The map label for this gene is folE

Identifier: 17546106

GI number: 17546106

Start: 1480199

End: 1480828

Strand: Direct

Name: folE

Synonym: RSc1387

Alternate gene names: 17546106

Gene position: 1480199-1480828 (Clockwise)

Preceding gene: 17546105

Following gene: 17546114

Centisome position: 39.83

GC content: 67.78

Gene sequence:

>630_bases
ATGCTGTGTTCACATGACGACGACGACCCGATCCGGACAGCGGCCAGCCACGGCATCCGGCGCGTCCGTGCGTTCGATGC
GCAGCGATTCGAGCTGGCCGTCCAGAGCCTGCTCGAGGCGAGCGGCATCGAGATCGATACCGCGCACACGGGCAAGACGG
CCCAGCGCGTGCGCGAGCTTTGGCAGCGGCGCCTGCTGGACGGCTACGACATCGATCCCGCCGAGGCGCTGGGCCACGGC
TTCGAGGATCCGCGCCGCGATATGGTCATCATCCGCAGCATTGCGGTGCACGGCGTCTGTCCGCACCATCTGCTGCCGTT
TCGGGGTGTCGCGCACGTGGCCTACCTGCCCGATGGGCGGCTGCACGGTTTCGGCCGCATCGCGCGCATGATCGATGCGA
TCAGCCACCGCTACACCTACCAGGAGTGGGTGACGAACGAGGTCGCGCATGCCCTGGTCGCGCACGGCCAGGCGCGCGGC
GCGGCGTGCCTGATCGAAGCCGAGCAGCTCTGCCTGCTGATGGGCGAGAACCGGCGCGGCGACGAGCGCGTGATTACCCA
GTGCTATGTCGGGGAATTCGAGCAGAACATGCAGGCCCGGACGGAATTCCTGCGCGCGATCAAGGGCTGA

Upstream 100 bases:

>100_bases
GCCCGCCGGGCAATCGAGTCCATATAATCGACAGATCATCTTCGGTACGCGGTGATCGTGATGCGGTGGATCGATCTCCG
GCCGATTTGGAGGAAAGCAA

Downstream 100 bases:

>100_bases
CGCGCGCGGCGATGACGGCGGGCGCATCGCCTCGGCGCGCCCAGCGCCGCCGGCCGCCGATCACGGCCTGCGGGCAAGCG
GACGGACAACGCCGGCCCGC

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MLCSHDDDDPIRTAASHGIRRVRAFDAQRFELAVQSLLEASGIEIDTAHTGKTAQRVRELWQRRLLDGYDIDPAEALGHG
FEDPRRDMVIIRSIAVHGVCPHHLLPFRGVAHVAYLPDGRLHGFGRIARMIDAISHRYTYQEWVTNEVAHALVAHGQARG
AACLIEAEQLCLLMGENRRGDERVITQCYVGEFEQNMQARTEFLRAIKG

Sequences:

>Translated_209_residues
MLCSHDDDDPIRTAASHGIRRVRAFDAQRFELAVQSLLEASGIEIDTAHTGKTAQRVRELWQRRLLDGYDIDPAEALGHG
FEDPRRDMVIIRSIAVHGVCPHHLLPFRGVAHVAYLPDGRLHGFGRIARMIDAISHRYTYQEWVTNEVAHALVAHGQARG
AACLIEAEQLCLLMGENRRGDERVITQCYVGEFEQNMQARTEFLRAIKG
>Mature_209_residues
MLCSHDDDDPIRTAASHGIRRVRAFDAQRFELAVQSLLEASGIEIDTAHTGKTAQRVRELWQRRLLDGYDIDPAEALGHG
FEDPRRDMVIIRSIAVHGVCPHHLLPFRGVAHVAYLPDGRLHGFGRIARMIDAISHRYTYQEWVTNEVAHALVAHGQARG
AACLIEAEQLCLLMGENRRGDERVITQCYVGEFEQNMQARTEFLRAIKG

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=129, Percent_Identity=30.2325581395349, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI4503949, Length=129, Percent_Identity=30.2325581395349, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI66932970, Length=110, Percent_Identity=28.1818181818182, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI66932972, Length=87, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560486, Length=176, Percent_Identity=31.8181818181818, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321706, Length=182, Percent_Identity=28.021978021978, Blast_Score=89, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24656796, Length=143, Percent_Identity=29.3706293706294, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24656782, Length=142, Percent_Identity=29.5774647887324, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24656791, Length=143, Percent_Identity=29.3706293706294, Blast_Score=81, Evalue=4e-16,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_RALSO (Q8XZK8)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519508.1
- ProteinModelPortal:   Q8XZK8
- SMR:   Q8XZK8
- GeneID:   1220211
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1387
- NMPDR:   fig|267608.1.peg.1387
- HOGENOM:   HBG370191
- OMA:   RELWQKR
- ProtClustDB:   PRK09347
- BioCyc:   RSOL267608:RSC1387-MONOMER
- BRENDA:   3.5.4.16
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 23574; Mature: 23574

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCSHDDDDPIRTAASHGIRRVRAFDAQRFELAVQSLLEASGIEIDTAHTGKTAQRVREL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHH
WQRRLLDGYDIDPAEALGHGFEDPRRDMVIIRSIAVHGVCPHHLLPFRGVAHVAYLPDGR
HHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHCCCCCCHHHEEECCCCC
LHGFGRIARMIDAISHRYTYQEWVTNEVAHALVAHGQARGAACLIEAEQLCLLMGENRRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCC
DERVITQCYVGEFEQNMQARTEFLRAIKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLCSHDDDDPIRTAASHGIRRVRAFDAQRFELAVQSLLEASGIEIDTAHTGKTAQRVREL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHH
WQRRLLDGYDIDPAEALGHGFEDPRRDMVIIRSIAVHGVCPHHLLPFRGVAHVAYLPDGR
HHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHCCCCCCHHHEEECCCCC
LHGFGRIARMIDAISHRYTYQEWVTNEVAHALVAHGQARGAACLIEAEQLCLLMGENRRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCC
DERVITQCYVGEFEQNMQARTEFLRAIKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852