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The map label for this gene is iaaA [H]

Identifier: 17546097

GI number: 17546097

Start: 1468578

End: 1469540

Strand: Direct

Name: iaaA [H]

Synonym: RSc1378

Alternate gene names: 17546097

Gene position: 1468578-1469540 (Clockwise)

Preceding gene: 17546096

Following gene: 17546098

Centisome position: 39.52

GC content: 72.69

Gene sequence:

>963_bases
ATGCCCACCCCGATCCTCGCCATCCATGGCGGCGCCGGCACCATTACCCGGGCGGCGATGGACGCCGACAAGGAAGCCGC
CTACCGGCAGGCGCTCGACGGCATCCTGCTGGCCGGCCAGCGCATTCTGGCCGGCGGCGGCAGCGCGCTCGACGCCGTGA
CCGAAGCCGTGCGCCTGCTGGAAGCGTGCCCGCTGTTCAATGCCGGCAAGGGGTCCGTGCTCACGCGCGCGGGCACCTAC
GAGCTGGATGCCTCGATCATGGACGGCACCACGCTGGCCGCCGGCGCGGTGACCTGCGTCAAGCGCCTGCGCCATCCCGT
GCTGGCCGCGCGCGCCGTGATGGAGCACAGCGAGCACGTGCTGTTCACCTCCGAGGGCGCCGAGGCGTTCGCCGCGTCGC
AGGGACTGGAGTTCGTCGAGCCGGACTACTACTACACCGAGGCGCGCTACGCCCAGTGGCAGCGCGCCCGCCAGCACGCC
GGCATGGCGCTGCTCGACCACGATGCGGCATCCCTGCTGGCGCAGGCGCCCGAGCCGATCGATCCCGACAGCAAGTTCGG
CACCGTGGGCGCGGTCGCGTGCGATGCGCAGGGCCGGCTGGCGGCGGCCACGTCCACCGGCGGCGTCACCAACAAGCAGG
TCGGCCGGGTGGGCGACACGCCGCTGATCGGCGCCGGCTGCTACGCCAACCGCGCCGCCGCCGTGTCGTGCACCGGCACC
GGGGAGATGTTCATCCGCGCCGTGGCCGCCTACGACGTGGCCGCGCAGATGGAATACGCCGGCAAGCCGCTGGCCGAGGC
CTGCGACGACGTGGTGATGCGCAAGCTGGTCGCCATCGGGGGGCGCGGCGGGCTGATCGCGGTGGACGCGCACAGCAACG
TGGCGCTGCCGTTCAACACCGAGGGCATGTACCGGGGCTTCGCGCGCGGGGATGCCGCGCCCGAGGTCTCGATTTACCGA
TAG

Upstream 100 bases:

>100_bases
CTGCGCCTGCCGACACGGCCCGCGTGGCCACGGCCACCACCCGGCGCGGCCGCAAGCGCGCGTCGGCCGACTGATCCCGA
GATTGCCGAGACCGACCCGT

Downstream 100 bases:

>100_bases
CGGACCGCGCCCGACCGCGTGGCCGCAAGCAGGAGCACCACCGTGACAGCAACCCAGCAGACCGAGACCGCCATCGCGAT
GCCCGAGCAGCGCATCGTGC

Product: L-asparaginase precursor protein

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MPTPILAIHGGAGTITRAAMDADKEAAYRQALDGILLAGQRILAGGGSALDAVTEAVRLLEACPLFNAGKGSVLTRAGTY
ELDASIMDGTTLAAGAVTCVKRLRHPVLAARAVMEHSEHVLFTSEGAEAFAASQGLEFVEPDYYYTEARYAQWQRARQHA
GMALLDHDAASLLAQAPEPIDPDSKFGTVGAVACDAQGRLAAATSTGGVTNKQVGRVGDTPLIGAGCYANRAAAVSCTGT
GEMFIRAVAAYDVAAQMEYAGKPLAEACDDVVMRKLVAIGGRGGLIAVDAHSNVALPFNTEGMYRGFARGDAAPEVSIYR

Sequences:

>Translated_320_residues
MPTPILAIHGGAGTITRAAMDADKEAAYRQALDGILLAGQRILAGGGSALDAVTEAVRLLEACPLFNAGKGSVLTRAGTY
ELDASIMDGTTLAAGAVTCVKRLRHPVLAARAVMEHSEHVLFTSEGAEAFAASQGLEFVEPDYYYTEARYAQWQRARQHA
GMALLDHDAASLLAQAPEPIDPDSKFGTVGAVACDAQGRLAAATSTGGVTNKQVGRVGDTPLIGAGCYANRAAAVSCTGT
GEMFIRAVAAYDVAAQMEYAGKPLAEACDDVVMRKLVAIGGRGGLIAVDAHSNVALPFNTEGMYRGFARGDAAPEVSIYR
>Mature_319_residues
PTPILAIHGGAGTITRAAMDADKEAAYRQALDGILLAGQRILAGGGSALDAVTEAVRLLEACPLFNAGKGSVLTRAGTYE
LDASIMDGTTLAAGAVTCVKRLRHPVLAARAVMEHSEHVLFTSEGAEAFAASQGLEFVEPDYYYTEARYAQWQRARQHAG
MALLDHDAASLLAQAPEPIDPDSKFGTVGAVACDAQGRLAAATSTGGVTNKQVGRVGDTPLIGAGCYANRAAAVSCTGTG
EMFIRAVAAYDVAAQMEYAGKPLAEACDDVVMRKLVAIGGRGGLIAVDAHSNVALPFNTEGMYRGFARGDAAPEVSIYR

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=303, Percent_Identity=38.9438943894389, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI145275200, Length=303, Percent_Identity=38.9438943894389, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI98991769, Length=284, Percent_Identity=32.3943661971831, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI285002251, Length=255, Percent_Identity=34.1176470588235, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI285002253, Length=255, Percent_Identity=32.156862745098, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787050, Length=321, Percent_Identity=59.8130841121495, Blast_Score=361, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17569239, Length=271, Percent_Identity=27.3062730627306, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI18921183, Length=315, Percent_Identity=39.0476190476191, Blast_Score=174, Evalue=8e-44,
Organism=Drosophila melanogaster, GI21357893, Length=269, Percent_Identity=32.3420074349442, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI221330102, Length=248, Percent_Identity=31.4516129032258, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24652135, Length=248, Percent_Identity=31.4516129032258, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24655553, Length=221, Percent_Identity=29.4117647058824, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 33191; Mature: 33060

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPILAIHGGAGTITRAAMDADKEAAYRQALDGILLAGQRILAGGGSALDAVTEAVRLL
CCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHH
EACPLFNAGKGSVLTRAGTYELDASIMDGTTLAAGAVTCVKRLRHPVLAARAVMEHSEHV
HHCCCCCCCCCCEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LFTSEGAEAFAASQGLEFVEPDYYYTEARYAQWQRARQHAGMALLDHDAASLLAQAPEPI
EEECCCCHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHCCEEEECCHHHHHHHCCCCCC
DPDSKFGTVGAVACDAQGRLAAATSTGGVTNKQVGRVGDTPLIGAGCYANRAAAVSCTGT
CCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEECCC
GEMFIRAVAAYDVAAQMEYAGKPLAEACDDVVMRKLVAIGGRGGLIAVDAHSNVALPFNT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECC
EGMYRGFARGDAAPEVSIYR
CHHHCCCCCCCCCCCCEEEC
>Mature Secondary Structure 
PTPILAIHGGAGTITRAAMDADKEAAYRQALDGILLAGQRILAGGGSALDAVTEAVRLL
CCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHH
EACPLFNAGKGSVLTRAGTYELDASIMDGTTLAAGAVTCVKRLRHPVLAARAVMEHSEHV
HHCCCCCCCCCCEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LFTSEGAEAFAASQGLEFVEPDYYYTEARYAQWQRARQHAGMALLDHDAASLLAQAPEPI
EEECCCCHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHCCEEEECCHHHHHHHCCCCCC
DPDSKFGTVGAVACDAQGRLAAATSTGGVTNKQVGRVGDTPLIGAGCYANRAAAVSCTGT
CCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEECCC
GEMFIRAVAAYDVAAQMEYAGKPLAEACDDVVMRKLVAIGGRGGLIAVDAHSNVALPFNT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECC
EGMYRGFARGDAAPEVSIYR
CHHHCCCCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]