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The map label for this gene is yhcA [H]

Identifier: 17546084

GI number: 17546084

Start: 1454365

End: 1455828

Strand: Direct

Name: yhcA [H]

Synonym: RSc1365

Alternate gene names: 17546084

Gene position: 1454365-1455828 (Clockwise)

Preceding gene: 17546083

Following gene: 17546086

Centisome position: 39.13

GC content: 70.9

Gene sequence:

>1464_bases
ATGGCGACCTTCACGCCCACGCTGGAAACCCTGCGCGCGCGCCATGGCGAGCGCTTCAAGTGGCTGGTGCTGTTCACGCT
GACGGTGGGCGCGGTGTCGTCGATCATTTCCGCCACCATCGTCAACGTGGCGATTCCCGATCTGAGCCGGCATTTCGTGC
TGGGCCAGGAGCGCGCGCAGTGGGTTTCGGCCAGCTTCATGGTGGCGATGACGCTGGCGATGCTGCTCACGCCGTGGCTG
CTGCTGCGCTTCGGGCTGCGGCGCACCTTCATCGGCGCGCTGCTGTTGCTGGGCGTGGGCGGGCTGGTCGGCGGGCTGTC
GCCCACCTACGGCGTGATGATCGCCATGCGGGTGGTGGGGGGCGTGGCGGCGGGCATCATGCAGCCGCTGCCCAATATCC
TGATCCTGCGCGTGTTTCCGGAGCGCGAGCAGGGCAAGGCGTTCGGGCTGTTCGGCTTCGGCGTGGTGCTGGCGCCGGCG
CTCGGGCCCAGCCTTGGCGGCTTCCTGGTGGAGCTGTTCGGCTGGCGCTCGATCTTCTTCGTGGTGGTGCCATTCACGCT
GCTGGGCTGGGCGATGGCGCGCCGTTTCATGGCGATCAACTCGTCGATGGCCGGCGAGCCCAAGCCGCTCGACTGGCGCG
GCCTGCTGCTGGTGGGCGCGGCGACGGTCGCGCTGCTCAACGGCCTGGTCGAACTGCACGCCGATGTCACGCGCGGCGTC
GCGCTGATGGCCGTGTCGGCGGTGTGCCTGGCGGTGTTCCTGTTCTGGCAGCGCCGCGTGGAATCGCCGCTGCTCGATCT
GCGGCTGTTCAGCTACCGGCAGTTCGCCATGGGCGCCGTGGTGGCGTTCATCTACGGCGCGGGGCTGTATGGCTCGACCT
ACCTGTTGCCGGTGTACATGCAGGTGGCGCTCGCCTACACGCCGTCGAGCGCCGGGCTGGTGCTGCTGCCGCCCGGGCTG
GCGCTGGCCGCGACGATCGTCGTGGCCGGGCGCCTGACCAGCCGGATCGAGCCGTACCGGCTGGTGTCGTTCGGGCTGGC
GGCGCTGGCCGTGTCCTTCCTGCTGATGACGACCAACACCCGCGCCACCGGCTATCTGCTGCTGATCGGCATCGCCGCGA
TCGGGCGGGTCGGGCTGGGCTTCGTGCTGCCGTCGCTGAGCCTGGGCGCCATGCGCGGGGTCGATTTCACGCTGATCCCG
CAGGGCTCCAGCGCCGTCAACTTCCTGCGCCAGCTGGGCGGGGCGATCGGCGTGTCGGCCACGGGCATCTTCCTGCAATG
GCGGCTGGCCGCGCGCGGCATCGGTGCGGTGGGCGGCGTGGCGGTGGACCCGGAGGCGCGCATCGTGGCGTGCGACGAGA
CCTTCGTCTTTCTGGGCGTGCTGTGCGCGCTGGCGGTGGCGGCGGCCTGGCGCATGCGCCGGCGCGAGCCGGCCGCCGTG
GTGTCGGCCGGCGTGGGGCCATAA

Upstream 100 bases:

>100_bases
CTGATCGGCATCCCGCCCGGCCGGCGCGCGGAGGCCGCGCCGGCCGGGTTGGCGCTTCTGCCGGCTCCATTTCCGTAACA
CATCCAATAACAACGAGAGC

Downstream 100 bases:

>100_bases
AAAAGCCGGGCGCTCGGCCCGGCCATGTGCCATGTGCGCGGCGGTCGGCGATCAGGTCGTCGCGCCGCTCTTCAGTTCTT
CGACGAGATCAATGTACTTC

Product: multidrug resistance-like efflux transmembrane protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MATFTPTLETLRARHGERFKWLVLFTLTVGAVSSIISATIVNVAIPDLSRHFVLGQERAQWVSASFMVAMTLAMLLTPWL
LLRFGLRRTFIGALLLLGVGGLVGGLSPTYGVMIAMRVVGGVAAGIMQPLPNILILRVFPEREQGKAFGLFGFGVVLAPA
LGPSLGGFLVELFGWRSIFFVVVPFTLLGWAMARRFMAINSSMAGEPKPLDWRGLLLVGAATVALLNGLVELHADVTRGV
ALMAVSAVCLAVFLFWQRRVESPLLDLRLFSYRQFAMGAVVAFIYGAGLYGSTYLLPVYMQVALAYTPSSAGLVLLPPGL
ALAATIVVAGRLTSRIEPYRLVSFGLAALAVSFLLMTTNTRATGYLLLIGIAAIGRVGLGFVLPSLSLGAMRGVDFTLIP
QGSSAVNFLRQLGGAIGVSATGIFLQWRLAARGIGAVGGVAVDPEARIVACDETFVFLGVLCALAVAAAWRMRRREPAAV
VSAGVGP

Sequences:

>Translated_487_residues
MATFTPTLETLRARHGERFKWLVLFTLTVGAVSSIISATIVNVAIPDLSRHFVLGQERAQWVSASFMVAMTLAMLLTPWL
LLRFGLRRTFIGALLLLGVGGLVGGLSPTYGVMIAMRVVGGVAAGIMQPLPNILILRVFPEREQGKAFGLFGFGVVLAPA
LGPSLGGFLVELFGWRSIFFVVVPFTLLGWAMARRFMAINSSMAGEPKPLDWRGLLLVGAATVALLNGLVELHADVTRGV
ALMAVSAVCLAVFLFWQRRVESPLLDLRLFSYRQFAMGAVVAFIYGAGLYGSTYLLPVYMQVALAYTPSSAGLVLLPPGL
ALAATIVVAGRLTSRIEPYRLVSFGLAALAVSFLLMTTNTRATGYLLLIGIAAIGRVGLGFVLPSLSLGAMRGVDFTLIP
QGSSAVNFLRQLGGAIGVSATGIFLQWRLAARGIGAVGGVAVDPEARIVACDETFVFLGVLCALAVAAAWRMRRREPAAV
VSAGVGP
>Mature_486_residues
ATFTPTLETLRARHGERFKWLVLFTLTVGAVSSIISATIVNVAIPDLSRHFVLGQERAQWVSASFMVAMTLAMLLTPWLL
LRFGLRRTFIGALLLLGVGGLVGGLSPTYGVMIAMRVVGGVAAGIMQPLPNILILRVFPEREQGKAFGLFGFGVVLAPAL
GPSLGGFLVELFGWRSIFFVVVPFTLLGWAMARRFMAINSSMAGEPKPLDWRGLLLVGAATVALLNGLVELHADVTRGVA
LMAVSAVCLAVFLFWQRRVESPLLDLRLFSYRQFAMGAVVAFIYGAGLYGSTYLLPVYMQVALAYTPSSAGLVLLPPGLA
LAATIVVAGRLTSRIEPYRLVSFGLAALAVSFLLMTTNTRATGYLLLIGIAAIGRVGLGFVLPSLSLGAMRGVDFTLIPQ
GSSAVNFLRQLGGAIGVSATGIFLQWRLAARGIGAVGGVAVDPEARIVACDETFVFLGVLCALAVAAAWRMRRREPAAVV
SAGVGP

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=410, Percent_Identity=28.2926829268293, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1788710, Length=415, Percent_Identity=28.6746987951807, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1790195, Length=291, Percent_Identity=30.2405498281787, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081983, Length=338, Percent_Identity=25.1479289940828, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788392, Length=417, Percent_Identity=26.1390887290168, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323735, Length=324, Percent_Identity=25.3086419753086, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51627; Mature: 51496

Theoretical pI: Translated: 11.33; Mature: 11.33

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATFTPTLETLRARHGERFKWLVLFTLTVGAVSSIISATIVNVAIPDLSRHFVLGQERAQ
CCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHH
WVSASFMVAMTLAMLLTPWLLLRFGLRRTFIGALLLLGVGGLVGGLSPTYGVMIAMRVVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GVAAGIMQPLPNILILRVFPEREQGKAFGLFGFGVVLAPALGPSLGGFLVELFGWRSIFF
HHHHHHHHCCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VVVPFTLLGWAMARRFMAINSSMAGEPKPLDWRGLLLVGAATVALLNGLVELHADVTRGV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALMAVSAVCLAVFLFWQRRVESPLLDLRLFSYRQFAMGAVVAFIYGAGLYGSTYLLPVYM
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
QVALAYTPSSAGLVLLPPGLALAATIVVAGRLTSRIEPYRLVSFGLAALAVSFLLMTTNT
HHHHEECCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
RATGYLLLIGIAAIGRVGLGFVLPSLSLGAMRGVDFTLIPQGSSAVNFLRQLGGAIGVSA
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEECCCCHHHHHHHHHCCCCCCCH
TGIFLQWRLAARGIGAVGGVAVDPEARIVACDETFVFLGVLCALAVAAAWRMRRREPAAV
HHHEEEEEHHHCCCCCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VSAGVGP
HCCCCCC
>Mature Secondary Structure 
ATFTPTLETLRARHGERFKWLVLFTLTVGAVSSIISATIVNVAIPDLSRHFVLGQERAQ
CCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHH
WVSASFMVAMTLAMLLTPWLLLRFGLRRTFIGALLLLGVGGLVGGLSPTYGVMIAMRVVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GVAAGIMQPLPNILILRVFPEREQGKAFGLFGFGVVLAPALGPSLGGFLVELFGWRSIFF
HHHHHHHHCCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VVVPFTLLGWAMARRFMAINSSMAGEPKPLDWRGLLLVGAATVALLNGLVELHADVTRGV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALMAVSAVCLAVFLFWQRRVESPLLDLRLFSYRQFAMGAVVAFIYGAGLYGSTYLLPVYM
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
QVALAYTPSSAGLVLLPPGLALAATIVVAGRLTSRIEPYRLVSFGLAALAVSFLLMTTNT
HHHHEECCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
RATGYLLLIGIAAIGRVGLGFVLPSLSLGAMRGVDFTLIPQGSSAVNFLRQLGGAIGVSA
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEECCCCHHHHHHHHHCCCCCCCH
TGIFLQWRLAARGIGAVGGVAVDPEARIVACDETFVFLGVLCALAVAAAWRMRRREPAAV
HHHEEEEEHHHCCCCCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VSAGVGP
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]