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The map label for this gene is pucI [H]

Identifier: 17545952

GI number: 17545952

Start: 1304554

End: 1306062

Strand: Direct

Name: pucI [H]

Synonym: RSc1233

Alternate gene names: 17545952

Gene position: 1304554-1306062 (Clockwise)

Preceding gene: 17545951

Following gene: 17545953

Centisome position: 35.1

GC content: 67.53

Gene sequence:

>1509_bases
ATGAAGCCGACAGCGCAACCCGCCGATGCCCAGCCGGGCGCGGCGCACGGCAGCCGTCTCTACAACGACGATCTGGCGCC
GACCGGGGCAGCGCAGCGCACCTGGAAGTGGTATCACTTCGCGGCGCTGTGGGTCGGCATGGTGATGAACATCGCCTCGT
ACATGCTGGCGGCCGGACTGACGGAGCAGGGCATGTCGCCGTGGCAGGCGGTGCTGACGGTGTTGCTGGGCAACGCCATC
GTGCTGGTGCCGATGTTGCTGATCGGCCATGCGGGGGCCAAGCACGGCATTCCGTACGCGGTGCTGGTGCGCACCTCGTT
CGGCACGCAGGGGGCGAAGCTGCCGGCGCTGCTGCGCGCCATCGTCGCGTGCGGCTGGTATGGCATCCAGACCTGGCTGG
GCGGCAGCGCCATCCATACGCTGCTCAACATCCTGACCGGCAATGCGCTGCACGGTGCGCCGCTGCCGCTGCTGGACATC
TCCGTCGCACAGCTGGCCTGCTTCCTGCTGTTCTGGGCGCTGCAGCTGTACTTCATCGTGCACGGCACTGACTCGATCCG
CTGGCTGGAGAGCTGGTCGGCGCCGATCAAGATCGTGATGTGCATCGCGCTGGTGTGGTGGGTCACCGCGAAGGCGGGCG
GGGTCGGCTCGATGCTGTCGGCGCCGTCGCAGTTCACGGCGGGCGGCAAGAAGGCCGGGCTGTTCTGGGCGACCTTCTGG
CCGAGCCTGACGGCGATGGTCGGCTTCTGGGCGACGCTGGCGCTGAACATTCCGGACTTCACACGCTTCGCCAGATCGCA
GCGGGACCAGGTGGTGGGCCAGTCGATCGGGCTGCCGGTGCCGATGGCGCTGCTGTCGGTGATCTCGGTGGTGGTGACCT
CGGCCACGGTGGTGATCTACGGCCAGGCGGTCTGGGACCCGATCGACCTGACCAGCCGCATGACGGGCATCGGCGTGGGC
ATCGCGCTGGTGATCCTGACGCTGGACACCATGTGCTGCAACCTGGCCGCCAACCTGGTGGGGCCGGCCTACGATTTCTC
CAGCCTGTGGCCGAAGGGCATCTCGTACAAGACGGGCGGGCTCATCACCGCGACCATCGCCATCGTGATGATGCCGTGGA
AGATCCTGGCCACCACGCAGGGCTACATCTTCACGTGGCTGGTCGGCTATTCGGCGCTGCTGGGGCCGGTGGCGGGGATC
ATGATGGTCGACTACCTCCTGATTCGCGGCACGCGGCTCGAGCCACGCGCGCTGTTCGACGAGCGCGGCGAGTATGCCTA
CACCGGCGGCTGGAACCTCGGCGCGGTGGCGGCGCTGGCGGTGGGCGTGCTGCCCAATCTGCCCGGCTTTCTGCATACCG
CGTTTCCGGCGTCGTTCCCGAACGTGCCGGCGTTCTTCAATACGCTCTACACCTATGCGTGGTTCGTCGGGCTGGTGCTG
GCGTCCATTGTCTATGGCGCGTGGATGAAGCTGCGCGGCAGTCCGCGTGCGCGCGTGGCAAGCGCCTGA

Upstream 100 bases:

>100_bases
GTGCTTGACCGGATGGACGCATCCCCGTGGTTTTTGCTATCTACACATGAGCCTGACGCGCGCCGCAGCGCCCGCGCCCA
ACATCCGTGGAGGTTTTTGA

Downstream 100 bases:

>100_bases
GCTGCCCGCCGCCGCCTGCGCGCGGATCTTCGCAAGGGCATCACGGGATGCTCCGCGCTGCGTCACCATGCACCCGCGTG
CAATACGTCGGGGATGGTCG

Product: transporter transmembrane protein

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MKPTAQPADAQPGAAHGSRLYNDDLAPTGAAQRTWKWYHFAALWVGMVMNIASYMLAAGLTEQGMSPWQAVLTVLLGNAI
VLVPMLLIGHAGAKHGIPYAVLVRTSFGTQGAKLPALLRAIVACGWYGIQTWLGGSAIHTLLNILTGNALHGAPLPLLDI
SVAQLACFLLFWALQLYFIVHGTDSIRWLESWSAPIKIVMCIALVWWVTAKAGGVGSMLSAPSQFTAGGKKAGLFWATFW
PSLTAMVGFWATLALNIPDFTRFARSQRDQVVGQSIGLPVPMALLSVISVVVTSATVVIYGQAVWDPIDLTSRMTGIGVG
IALVILTLDTMCCNLAANLVGPAYDFSSLWPKGISYKTGGLITATIAIVMMPWKILATTQGYIFTWLVGYSALLGPVAGI
MMVDYLLIRGTRLEPRALFDERGEYAYTGGWNLGAVAALAVGVLPNLPGFLHTAFPASFPNVPAFFNTLYTYAWFVGLVL
ASIVYGAWMKLRGSPRARVASA

Sequences:

>Translated_502_residues
MKPTAQPADAQPGAAHGSRLYNDDLAPTGAAQRTWKWYHFAALWVGMVMNIASYMLAAGLTEQGMSPWQAVLTVLLGNAI
VLVPMLLIGHAGAKHGIPYAVLVRTSFGTQGAKLPALLRAIVACGWYGIQTWLGGSAIHTLLNILTGNALHGAPLPLLDI
SVAQLACFLLFWALQLYFIVHGTDSIRWLESWSAPIKIVMCIALVWWVTAKAGGVGSMLSAPSQFTAGGKKAGLFWATFW
PSLTAMVGFWATLALNIPDFTRFARSQRDQVVGQSIGLPVPMALLSVISVVVTSATVVIYGQAVWDPIDLTSRMTGIGVG
IALVILTLDTMCCNLAANLVGPAYDFSSLWPKGISYKTGGLITATIAIVMMPWKILATTQGYIFTWLVGYSALLGPVAGI
MMVDYLLIRGTRLEPRALFDERGEYAYTGGWNLGAVAALAVGVLPNLPGFLHTAFPASFPNVPAFFNTLYTYAWFVGLVL
ASIVYGAWMKLRGSPRARVASA
>Mature_502_residues
MKPTAQPADAQPGAAHGSRLYNDDLAPTGAAQRTWKWYHFAALWVGMVMNIASYMLAAGLTEQGMSPWQAVLTVLLGNAI
VLVPMLLIGHAGAKHGIPYAVLVRTSFGTQGAKLPALLRAIVACGWYGIQTWLGGSAIHTLLNILTGNALHGAPLPLLDI
SVAQLACFLLFWALQLYFIVHGTDSIRWLESWSAPIKIVMCIALVWWVTAKAGGVGSMLSAPSQFTAGGKKAGLFWATFW
PSLTAMVGFWATLALNIPDFTRFARSQRDQVVGQSIGLPVPMALLSVISVVVTSATVVIYGQAVWDPIDLTSRMTGIGVG
IALVILTLDTMCCNLAANLVGPAYDFSSLWPKGISYKTGGLITATIAIVMMPWKILATTQGYIFTWLVGYSALLGPVAGI
MMVDYLLIRGTRLEPRALFDERGEYAYTGGWNLGAVAALAVGVLPNLPGFLHTAFPASFPNVPAFFNTLYTYAWFVGLVL
ASIVYGAWMKLRGSPRARVASA

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=481, Percent_Identity=29.3139293139293, Blast_Score=194, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6319429, Length=499, Percent_Identity=27.4549098196393, Blast_Score=192, Evalue=9e-50,
Organism=Saccharomyces cerevisiae, GI6322219, Length=453, Percent_Identity=27.3730684326711, Blast_Score=187, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6319495, Length=457, Percent_Identity=26.4770240700219, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6324645, Length=444, Percent_Identity=24.7747747747748, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6323266, Length=452, Percent_Identity=24.1150442477876, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324766, Length=446, Percent_Identity=23.3183856502242, Blast_Score=127, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 53784; Mature: 53784

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTAQPADAQPGAAHGSRLYNDDLAPTGAAQRTWKWYHFAALWVGMVMNIASYMLAAGL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TEQGMSPWQAVLTVLLGNAIVLVPMLLIGHAGAKHGIPYAVLVRTSFGTQGAKLPALLRA
HHCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH
IVACGWYGIQTWLGGSAIHTLLNILTGNALHGAPLPLLDISVAQLACFLLFWALQLYFIV
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHE
HGTDSIRWLESWSAPIKIVMCIALVWWVTAKAGGVGSMLSAPSQFTAGGKKAGLFWATFW
ECCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHCCCCCCCCEEHHHHH
PSLTAMVGFWATLALNIPDFTRFARSQRDQVVGQSIGLPVPMALLSVISVVVTSATVVIY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEE
GQAVWDPIDLTSRMTGIGVGIALVILTLDTMCCNLAANLVGPAYDFSSLWPKGISYKTGG
ECHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCC
LITATIAIVMMPWKILATTQGYIFTWLVGYSALLGPVAGIMMVDYLLIRGTRLEPRALFD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ERGEYAYTGGWNLGAVAALAVGVLPNLPGFLHTAFPASFPNVPAFFNTLYTYAWFVGLVL
CCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
ASIVYGAWMKLRGSPRARVASA
HHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKPTAQPADAQPGAAHGSRLYNDDLAPTGAAQRTWKWYHFAALWVGMVMNIASYMLAAGL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TEQGMSPWQAVLTVLLGNAIVLVPMLLIGHAGAKHGIPYAVLVRTSFGTQGAKLPALLRA
HHCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH
IVACGWYGIQTWLGGSAIHTLLNILTGNALHGAPLPLLDISVAQLACFLLFWALQLYFIV
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHE
HGTDSIRWLESWSAPIKIVMCIALVWWVTAKAGGVGSMLSAPSQFTAGGKKAGLFWATFW
ECCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHCCCCCCCCEEHHHHH
PSLTAMVGFWATLALNIPDFTRFARSQRDQVVGQSIGLPVPMALLSVISVVVTSATVVIY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEE
GQAVWDPIDLTSRMTGIGVGIALVILTLDTMCCNLAANLVGPAYDFSSLWPKGISYKTGG
ECHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCC
LITATIAIVMMPWKILATTQGYIFTWLVGYSALLGPVAGIMMVDYLLIRGTRLEPRALFD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ERGEYAYTGGWNLGAVAALAVGVLPNLPGFLHTAFPASFPNVPAFFNTLYTYAWFVGLVL
CCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
ASIVYGAWMKLRGSPRARVASA
HHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]