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The map label for this gene is fabI [H]

Identifier: 17545891

GI number: 17545891

Start: 1231104

End: 1231898

Strand: Reverse

Name: fabI [H]

Synonym: RSc1172

Alternate gene names: 17545891

Gene position: 1231898-1231104 (Counterclockwise)

Preceding gene: 17545897

Following gene: 17545885

Centisome position: 33.15

GC content: 64.91

Gene sequence:

>795_bases
ATGGGATTTCTGGCAGGCAAACGCATTCTGATCACCGGCCTTCTGTCCAACCGCTCGATCGCCTACGGCATCGCCAGCGC
ATGCAAGCGCGAGGGCGCCGAACTGGCTTTCACCTATGTCGGCGAGCGTTTCAAGGATCGCATCACCGAATTCGCCAACG
AGTTCGGTAGCCAGCTGGTGTTCGACTGCGACGTCGGCAGCGATGAGCAGATCGCCAAGGTGTTCGCCGATCTCGGCCAG
CACTGGGACCACTTCGACGGCCTGGTGCATTCGATCGGCTTCGCGCCGCGCGAGGCGATCGCCGGCGATTTCCTCGACGG
CCTGTCGCGCGAAGCGTTCCGCATCGCGCACGACATCTCGGCTTACAGCTTCCCCGCGCTGGCCAAGGCCGCGCTGCCGA
TGCTCTCGCCCAACGCGTCGCTGCTGGCATTGACCTACCTGGGCGCCGAGCGCGTGGTCCCGAACTACAACACGATGGGC
GTGGCCAAAGCCGCGCTCGAAGCCTGCGTACGCTATCTGGCGAGCGCCCTCGGCCCGAAGGGCATCCGCGCCAACGGCAT
CTCGGCCGGCCCGATCAAGACACTGGCCGCTTCGGGCATCAAGGACTTCGGCAAGCTGCTCAAGTACATGGAAGATGTCG
CGCCGTTGCGCCGCAACGTCACCATCGAAGAAGTCGGCAACGTGGCCGCCTTCCTGCTGTCGGACCTGTCCAGCGGCATG
ACGGGCGAGATCACCTATGTCGACTGCGGCTTCAACGTGACCGCAGGCGTGCCGGACGTCGCCGCCCAAGGCTGA

Upstream 100 bases:

>100_bases
TCCGGTATGATCGCGCCGACCGTGTCGCCCCGGCTCCCGCCGTGCGCCATCCCGCGCGATGCCCGGACGCGATGCTGCCC
CACATTTGTATGGAGAAACC

Downstream 100 bases:

>100_bases
TGCGCTGACACGCAAGCGCGCCATCTTCGGTTGGCGCGCTTTGCCGAAAGCAGCAAAGAAAAAAGCCCGCATGGCGCAAA
CCATGCGGGCTTTTTGACGT

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MGFLAGKRILITGLLSNRSIAYGIASACKREGAELAFTYVGERFKDRITEFANEFGSQLVFDCDVGSDEQIAKVFADLGQ
HWDHFDGLVHSIGFAPREAIAGDFLDGLSREAFRIAHDISAYSFPALAKAALPMLSPNASLLALTYLGAERVVPNYNTMG
VAKAALEACVRYLASALGPKGIRANGISAGPIKTLAASGIKDFGKLLKYMEDVAPLRRNVTIEEVGNVAAFLLSDLSSGM
TGEITYVDCGFNVTAGVPDVAAQG

Sequences:

>Translated_264_residues
MGFLAGKRILITGLLSNRSIAYGIASACKREGAELAFTYVGERFKDRITEFANEFGSQLVFDCDVGSDEQIAKVFADLGQ
HWDHFDGLVHSIGFAPREAIAGDFLDGLSREAFRIAHDISAYSFPALAKAALPMLSPNASLLALTYLGAERVVPNYNTMG
VAKAALEACVRYLASALGPKGIRANGISAGPIKTLAASGIKDFGKLLKYMEDVAPLRRNVTIEEVGNVAAFLLSDLSSGM
TGEITYVDCGFNVTAGVPDVAAQG
>Mature_263_residues
GFLAGKRILITGLLSNRSIAYGIASACKREGAELAFTYVGERFKDRITEFANEFGSQLVFDCDVGSDEQIAKVFADLGQH
WDHFDGLVHSIGFAPREAIAGDFLDGLSREAFRIAHDISAYSFPALAKAALPMLSPNASLLALTYLGAERVVPNYNTMGV
AKAALEACVRYLASALGPKGIRANGISAGPIKTLAASGIKDFGKLLKYMEDVAPLRRNVTIEEVGNVAAFLLSDLSSGMT
GEITYVDCGFNVTAGVPDVAAQG

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=255, Percent_Identity=61.5686274509804, Blast_Score=334, Evalue=4e-93,
Organism=Escherichia coli, GI1789378, Length=254, Percent_Identity=29.1338582677165, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787905, Length=251, Percent_Identity=27.0916334661355, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28006; Mature: 27875

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLAGKRILITGLLSNRSIAYGIASACKREGAELAFTYVGERFKDRITEFANEFGSQLV
CCCCCCCCEEEEEHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
FDCDVGSDEQIAKVFADLGQHWDHFDGLVHSIGFAPREAIAGDFLDGLSREAFRIAHDIS
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AYSFPALAKAALPMLSPNASLLALTYLGAERVVPNYNTMGVAKAALEACVRYLASALGPK
HHHHHHHHHHHHHCCCCCCCEEHHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GIRANGISAGPIKTLAASGIKDFGKLLKYMEDVAPLRRNVTIEEVGNVAAFLLSDLSSGM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
TGEITYVDCGFNVTAGVPDVAAQG
CCEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GFLAGKRILITGLLSNRSIAYGIASACKREGAELAFTYVGERFKDRITEFANEFGSQLV
CCCCCCCEEEEEHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
FDCDVGSDEQIAKVFADLGQHWDHFDGLVHSIGFAPREAIAGDFLDGLSREAFRIAHDIS
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AYSFPALAKAALPMLSPNASLLALTYLGAERVVPNYNTMGVAKAALEACVRYLASALGPK
HHHHHHHHHHHHHCCCCCCCEEHHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GIRANGISAGPIKTLAASGIKDFGKLLKYMEDVAPLRRNVTIEEVGNVAAFLLSDLSSGM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
TGEITYVDCGFNVTAGVPDVAAQG
CCEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]