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The map label for this gene is gph2 [H]

Identifier: 17545616

GI number: 17545616

Start: 947154

End: 947843

Strand: Reverse

Name: gph2 [H]

Synonym: RSc0897

Alternate gene names: 17545616

Gene position: 947843-947154 (Counterclockwise)

Preceding gene: 17545617

Following gene: 17545615

Centisome position: 25.5

GC content: 68.55

Gene sequence:

>690_bases
ATGACCACGACGAGGTTTCCCGATCCGCTGGGCGCGGTGCTGTTCGACCTGGACGGCACGCTGGCCGATACGGCGCCGGA
CCTGGCCGCAGCGGCCAACAAGGTCCGCGTGGATCGCGGCCTGGAACCGCTGGCCTACGAAGCGCTGCGGCCCATGGCGT
CGCATGGCGCCCGCGGCCTGATCGGCGTGGCATTCGGCATCGGGCCGGAAGATGCGGAATTCGAATCGCTCAAACACGCC
TTCCTGGCCAACTACGAGGCCGACATCTGCGTCCACACGCGGCTGTTTCCCGGCATGGACGACGTCCTGGCCGAGCTGGG
CCGCGCGGGCGTGCCATGGGGCATCGTGACCAACAAGCATGCGCGGTTCACCGTGCCGCTGGTGGCACAGCTGGCGTTCC
CGGTGCCGCCCGCCTGCGTGGTCAGCGGCGACACGACTCCGCACAGCAAGCCTCACCCCGCCCCGCTGCTGCATGCGGCC
GGCGCGATCGGCGTGCCGTCGCAGCAGGTCGTCTATGTTGGCGACGACGTACGTGATATCGAAGCGGGCCGTGCCGCCGG
CATGCCTACCATTGCCGCCAGTTACGGCTATTGTGGTAATGGGCCGCCCCCCGCGGAGTGGCAAGCCGATGCGCTGATCG
TGCACGCGACCGAATTGATTCCGCTGCTGTTCGCATCGCAGCGCGCCTGA

Upstream 100 bases:

>100_bases
AGTTGCGCGGCCTCGAATACAACCCGCTCACGGGCCGCTACGCGCTGACGCACGACACCAGCGTCAACTACCTGATAGCG
ACCCGCCGGCCGGACGCGCC

Downstream 100 bases:

>100_bases
GCGTGCCGACGAACCGGCACGCTCTTTCCGGGCCACCGGCTCTGCTGTGAAGCCACGGCCACGACTGACTGACGAAGCAC
GCCACGCCGTGGCCGGACCG

Product: hydrolase

Products: NA

Alternate protein names: PGP 2; PGPase2 [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTTTRFPDPLGAVLFDLDGTLADTAPDLAAAANKVRVDRGLEPLAYEALRPMASHGARGLIGVAFGIGPEDAEFESLKHA
FLANYEADICVHTRLFPGMDDVLAELGRAGVPWGIVTNKHARFTVPLVAQLAFPVPPACVVSGDTTPHSKPHPAPLLHAA
GAIGVPSQQVVYVGDDVRDIEAGRAAGMPTIAASYGYCGNGPPPAEWQADALIVHATELIPLLFASQRA

Sequences:

>Translated_229_residues
MTTTRFPDPLGAVLFDLDGTLADTAPDLAAAANKVRVDRGLEPLAYEALRPMASHGARGLIGVAFGIGPEDAEFESLKHA
FLANYEADICVHTRLFPGMDDVLAELGRAGVPWGIVTNKHARFTVPLVAQLAFPVPPACVVSGDTTPHSKPHPAPLLHAA
GAIGVPSQQVVYVGDDVRDIEAGRAAGMPTIAASYGYCGNGPPPAEWQADALIVHATELIPLLFASQRA
>Mature_228_residues
TTTRFPDPLGAVLFDLDGTLADTAPDLAAAANKVRVDRGLEPLAYEALRPMASHGARGLIGVAFGIGPEDAEFESLKHAF
LANYEADICVHTRLFPGMDDVLAELGRAGVPWGIVTNKHARFTVPLVAQLAFPVPPACVVSGDTTPHSKPHPAPLLHAAG
AIGVPSQQVVYVGDDVRDIEAGRAAGMPTIAASYGYCGNGPPPAEWQADALIVHATELIPLLFASQRA

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=240, Percent_Identity=32.9166666666667, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23987; Mature: 23855

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTRFPDPLGAVLFDLDGTLADTAPDLAAAANKVRVDRGLEPLAYEALRPMASHGARGL
CCCCCCCCCHHEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCE
IGVAFGIGPEDAEFESLKHAFLANYEADICVHTRLFPGMDDVLAELGRAGVPWGIVTNKH
EEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCEEECCC
ARFTVPLVAQLAFPVPPACVVSGDTTPHSKPHPAPLLHAAGAIGVPSQQVVYVGDDVRDI
CEEEHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHCCCCCCCCCEEEEECCCCHHH
EAGRAAGMPTIAASYGYCGNGPPPAEWQADALIVHATELIPLLFASQRA
HCCCCCCCCHHECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTTRFPDPLGAVLFDLDGTLADTAPDLAAAANKVRVDRGLEPLAYEALRPMASHGARGL
CCCCCCCCHHEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCE
IGVAFGIGPEDAEFESLKHAFLANYEADICVHTRLFPGMDDVLAELGRAGVPWGIVTNKH
EEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCEEECCC
ARFTVPLVAQLAFPVPPACVVSGDTTPHSKPHPAPLLHAAGAIGVPSQQVVYVGDDVRDI
CEEEHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHCCCCCCCCCEEEEECCCCHHH
EAGRAAGMPTIAASYGYCGNGPPPAEWQADALIVHATELIPLLFASQRA
HCCCCCCCCHHECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]