Click here to switch to the map view.

The map label for this gene is rhtB [H]

Identifier: 17545533

GI number: 17545533

Start: 858719

End: 859351

Strand: Reverse

Name: rhtB [H]

Synonym: RSc0814

Alternate gene names: 17545533

Gene position: 859351-858719 (Counterclockwise)

Preceding gene: 17545535

Following gene: 17545532

Centisome position: 23.12

GC content: 67.61

Gene sequence:

>633_bases
ATGCCCACCCTGACCAACCTCCTCACCTTCGCCCTGGTCGCCCTGGGCATGGTCCTGACCCCCGGCCCCAACATGATCTA
CCTGATCTCGCGGTCGATCAGCCAGGGGCGCAACGCGGGGCTGATTTCGCTGGCGGGCATCGCCACCGGGTTCGTGTTCT
ACATGCTGTGCGCGGCATTCGGCATCACGGCGCTGCTGATGGCGGTGCCGTTTGCCTACGACACGCTGCGCTTCGGCGGG
GCGATCTACCTGCTCTATCTGGCATGGCAGGCGGTGCGTCCCGGCGGGCGCGCCCCGTTCCAGCTGCGCGAGCTGCCTGC
GGACAGCCCGCGCCGCCTGTATTCGATGGGCCTGCTGACCAGCCTGCTCAACCCGAAGGTGGCGGTGCTGTATCTGTCGC
TGCTGCCGCAGTTCATCGAGCCGGCGCACGGCAGTGTGCTGGTGCAGTCGGTCTGCCTGGGGCTGACGCAGATCTGCGTG
AGCCTGACCATCAACTCGATGATCGCCTCGGCCGCCGGCAGCATCGCCGCGCTGCTTTCCCGCAAGGCGAGCTGGCTGGC
GCTCCAGCGTTACCTGATGGGCTCGGTGCTGACGGGGCTGGCGCTGCGCATGGCGATGGAAGCCCGCAAGTAA

Upstream 100 bases:

>100_bases
TACAGAATTCGCCAAATACCCAACGTTCTGTACCTGTCATGCGCCGTGGCATGGATGCTAGCCTGCAGGCTCATCTTTCC
GCCACCCGCAGCGCTCCGCC

Downstream 100 bases:

>100_bases
GCCCGCCGGATTGACATGGCGGTCGCCATGTCCTTGGATAGCTTGTAGGCCGCTACCAAGGAGACGACCGCATGGTTCCC
CCCGATCCGCCGCGACGCGC

Product: hypothetical protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MPTLTNLLTFALVALGMVLTPGPNMIYLISRSISQGRNAGLISLAGIATGFVFYMLCAAFGITALLMAVPFAYDTLRFGG
AIYLLYLAWQAVRPGGRAPFQLRELPADSPRRLYSMGLLTSLLNPKVAVLYLSLLPQFIEPAHGSVLVQSVCLGLTQICV
SLTINSMIASAAGSIAALLSRKASWLALQRYLMGSVLTGLALRMAMEARK

Sequences:

>Translated_210_residues
MPTLTNLLTFALVALGMVLTPGPNMIYLISRSISQGRNAGLISLAGIATGFVFYMLCAAFGITALLMAVPFAYDTLRFGG
AIYLLYLAWQAVRPGGRAPFQLRELPADSPRRLYSMGLLTSLLNPKVAVLYLSLLPQFIEPAHGSVLVQSVCLGLTQICV
SLTINSMIASAAGSIAALLSRKASWLALQRYLMGSVLTGLALRMAMEARK
>Mature_209_residues
PTLTNLLTFALVALGMVLTPGPNMIYLISRSISQGRNAGLISLAGIATGFVFYMLCAAFGITALLMAVPFAYDTLRFGGA
IYLLYLAWQAVRPGGRAPFQLRELPADSPRRLYSMGLLTSLLNPKVAVLYLSLLPQFIEPAHGSVLVQSVCLGLTQICVS
LTINSMIASAAGSIAALLSRKASWLALQRYLMGSVLTGLALRMAMEARK

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=175, Percent_Identity=30.2857142857143, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1788099, Length=206, Percent_Identity=28.1553398058252, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1786522, Length=118, Percent_Identity=27.9661016949153, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22486; Mature: 22355

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLTNLLTFALVALGMVLTPGPNMIYLISRSISQGRNAGLISLAGIATGFVFYMLCAAF
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
GITALLMAVPFAYDTLRFGGAIYLLYLAWQAVRPGGRAPFQLRELPADSPRRLYSMGLLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHH
SLLNPKVAVLYLSLLPQFIEPAHGSVLVQSVCLGLTQICVSLTINSMIASAAGSIAALLS
HHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKASWLALQRYLMGSVLTGLALRMAMEARK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PTLTNLLTFALVALGMVLTPGPNMIYLISRSISQGRNAGLISLAGIATGFVFYMLCAAF
CHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
GITALLMAVPFAYDTLRFGGAIYLLYLAWQAVRPGGRAPFQLRELPADSPRRLYSMGLLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHH
SLLNPKVAVLYLSLLPQFIEPAHGSVLVQSVCLGLTQICVSLTINSMIASAAGSIAALLS
HHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKASWLALQRYLMGSVLTGLALRMAMEARK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]