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The map label for this gene is tolB

Identifier: 17545454

GI number: 17545454

Start: 781673

End: 782971

Strand: Direct

Name: tolB

Synonym: RSc0735

Alternate gene names: 17545454

Gene position: 781673-782971 (Clockwise)

Preceding gene: 17545453

Following gene: 17545455

Centisome position: 21.03

GC content: 67.13

Gene sequence:

>1299_bases
ATGATGCGGAATGTTTGGAAGTCTGGACTTCGACGATCGGCCTGGATCGGGCTGCTGATGGTGTTGTGCGTCGGGGTTGC
GCGCGCCCAGCTGTATGTGGAAATCGCCGGGGCCGGTTCGAGCCAGTACCCCATCGCCATTGCCAATTTCCAGGGCGAAT
CGCAGATTCCGCAGGACATCACGGCCATCGTCCGGGCCGATCTGGTGCACAGCGGCCGCTTTTCCAATGTCGATGTGGCG
GGCGCGGTGGTGCCGGACAGCCCGGCCCCCGACCTGGGCGCCTGGAAGAGCCGTGGCGCGGCTGCGTTCGTCGGCGGCAC
CGTGACGCGCAACGGCGATCGCTACGAGATCCGCTTCCGCCTGTACGACACGGCCAAGGGGGAAAGCCTGGGCGGCCTGG
CGCTGACGCGCGGCGAAGGGCAACTGCGGCTGGCGGCGCATGAGATCGCCGACTACATCTACCAGAAGCTGATCGGTGAT
CGCGGTGTCTTTGCGACCCGCCTGTCGTATGTGTCCAAGGTGGGCCGCCGGTACCAGTTGCAGATTTCCGATTCCGACGG
GGCCAACCCGCAGGTGGCGCTGACCAGCAACGAGCCGATCATTTCGCCGGCGTGGTCGCCGGACGGCACCAAGGTCGCCT
ACGTGTCGTTCGAGAGCCGCAAGCCGGTGGTGTATGTGCATGACCTGATCTCGGGCCGCCGGGCGGTGATCTCGAACCAG
AAGGGCAACAACTCGGCGCCGGCCTGGTCGCCGGACGGGCGCCGCGTGGCGGTGGCGCTGTCGCGCGACGGCAATACGCA
GATCTACCAGGTCAACGCCGACGGCTCGGGCCTGCGCCGCCTGACCCGCAGCAGCGCGATCGATACCGAGCCGTCGTTCT
CGCCGGACGGCCGCTCGATCTACTTCACCAGCGACCGCGGCGGCGCGCCCCAGATCTATCGCATGCCGACCGAAGGCGAA
GACGCCGGCAGCGCGCAGCGCGTGACGTTCAAGGGCGGCTACAACACCAGCCCGCGCGTGTCGCCGGACGGCAAGCTGCT
GGCCTACATCTCGCGGGTGGGCGGGGCCTTCAAGCTGTATGTGCAGGACCTGTCCAATGGTGACGTCACCGGGCTGACCG
ACACGTCCTATGACGAATCGCCGAGCTTTGCCGCCAACGGCAAGTTCATCCTCTATGCCACCCGCGTAGGCGGGCGATCG
GTGCTGGCGGCGGTGTCAACCGACGGCCGCACGCGCCAGATTCTTTCCCTCCAGGCCGGCACGGTCCGCGAGCCGGCGTG
GGGCCCTTTCATGCAATAA

Upstream 100 bases:

>100_bases
ATGGCAAGGCGCCTGCGAGTGTGGTCGTCGGCTTCAAGCCCAAGCGGGGCTGATCGCCCGATACGCGGGTGAGGGATGCG
TTCAACAAGTTGTGAGGATG

Downstream 100 bases:

>100_bases
CGATTCCTTCAGGAGAGATCGAACATGCCGAAGTCTCAAACCATGATCAAGCTGGCGGCGATTGCCGCGCTGCTGGCGCT
GGGCGCTTGCAGCTCGGGTG

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MMRNVWKSGLRRSAWIGLLMVLCVGVARAQLYVEIAGAGSSQYPIAIANFQGESQIPQDITAIVRADLVHSGRFSNVDVA
GAVVPDSPAPDLGAWKSRGAAAFVGGTVTRNGDRYEIRFRLYDTAKGESLGGLALTRGEGQLRLAAHEIADYIYQKLIGD
RGVFATRLSYVSKVGRRYQLQISDSDGANPQVALTSNEPIISPAWSPDGTKVAYVSFESRKPVVYVHDLISGRRAVISNQ
KGNNSAPAWSPDGRRVAVALSRDGNTQIYQVNADGSGLRRLTRSSAIDTEPSFSPDGRSIYFTSDRGGAPQIYRMPTEGE
DAGSAQRVTFKGGYNTSPRVSPDGKLLAYISRVGGAFKLYVQDLSNGDVTGLTDTSYDESPSFAANGKFILYATRVGGRS
VLAAVSTDGRTRQILSLQAGTVREPAWGPFMQ

Sequences:

>Translated_432_residues
MMRNVWKSGLRRSAWIGLLMVLCVGVARAQLYVEIAGAGSSQYPIAIANFQGESQIPQDITAIVRADLVHSGRFSNVDVA
GAVVPDSPAPDLGAWKSRGAAAFVGGTVTRNGDRYEIRFRLYDTAKGESLGGLALTRGEGQLRLAAHEIADYIYQKLIGD
RGVFATRLSYVSKVGRRYQLQISDSDGANPQVALTSNEPIISPAWSPDGTKVAYVSFESRKPVVYVHDLISGRRAVISNQ
KGNNSAPAWSPDGRRVAVALSRDGNTQIYQVNADGSGLRRLTRSSAIDTEPSFSPDGRSIYFTSDRGGAPQIYRMPTEGE
DAGSAQRVTFKGGYNTSPRVSPDGKLLAYISRVGGAFKLYVQDLSNGDVTGLTDTSYDESPSFAANGKFILYATRVGGRS
VLAAVSTDGRTRQILSLQAGTVREPAWGPFMQ
>Mature_432_residues
MMRNVWKSGLRRSAWIGLLMVLCVGVARAQLYVEIAGAGSSQYPIAIANFQGESQIPQDITAIVRADLVHSGRFSNVDVA
GAVVPDSPAPDLGAWKSRGAAAFVGGTVTRNGDRYEIRFRLYDTAKGESLGGLALTRGEGQLRLAAHEIADYIYQKLIGD
RGVFATRLSYVSKVGRRYQLQISDSDGANPQVALTSNEPIISPAWSPDGTKVAYVSFESRKPVVYVHDLISGRRAVISNQ
KGNNSAPAWSPDGRRVAVALSRDGNTQIYQVNADGSGLRRLTRSSAIDTEPSFSPDGRSIYFTSDRGGAPQIYRMPTEGE
DAGSAQRVTFKGGYNTSPRVSPDGKLLAYISRVGGAFKLYVQDLSNGDVTGLTDTSYDESPSFAANGKFILYATRVGGRS
VLAAVSTDGRTRQILSLQAGTVREPAWGPFMQ

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=425, Percent_Identity=37.4117647058824, Blast_Score=263, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_RALSO (Q8Y1F5)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518856.1
- ProteinModelPortal:   Q8Y1F5
- SMR:   Q8Y1F5
- GeneID:   1219541
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0735
- NMPDR:   fig|267608.1.peg.735
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- ProtClustDB:   PRK02889
- BioCyc:   RSOL267608:RSC0735-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 46445; Mature: 46445

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRNVWKSGLRRSAWIGLLMVLCVGVARAQLYVEIAGAGSSQYPIAIANFQGESQIPQDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCEEEEECCCCCCCHHHH
TAIVRADLVHSGRFSNVDVAGAVVPDSPAPDLGAWKSRGAAAFVGGTVTRNGDRYEIRFR
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHCCCCEEEECCEEECCCCEEEEEEE
LYDTAKGESLGGLALTRGEGQLRLAAHEIADYIYQKLIGDRGVFATRLSYVSKVGRRYQL
EEECCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEE
QISDSDGANPQVALTSNEPIISPAWSPDGTKVAYVSFESRKPVVYVHDLISGRRAVISNQ
EECCCCCCCCEEEEECCCCEECCCCCCCCCEEEEEEECCCCCEEEEEEHHCCCEEEEECC
KGNNSAPAWSPDGRRVAVALSRDGNTQIYQVNADGSGLRRLTRSSAIDTEPSFSPDGRSI
CCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCEE
YFTSDRGGAPQIYRMPTEGEDAGSAQRVTFKGGYNTSPRVSPDGKLLAYISRVGGAFKLY
EEECCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCEEEEE
VQDLSNGDVTGLTDTSYDESPSFAANGKFILYATRVGGRSVLAAVSTDGRTRQILSLQAG
EEECCCCCEECCCCCCCCCCCCEECCCEEEEEEEECCCCEEEEEECCCCCCEEEEEECCC
TVREPAWGPFMQ
CCCCCCCCCCCC
>Mature Secondary Structure
MMRNVWKSGLRRSAWIGLLMVLCVGVARAQLYVEIAGAGSSQYPIAIANFQGESQIPQDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCEEEEECCCCCCCHHHH
TAIVRADLVHSGRFSNVDVAGAVVPDSPAPDLGAWKSRGAAAFVGGTVTRNGDRYEIRFR
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHCCCCEEEECCEEECCCCEEEEEEE
LYDTAKGESLGGLALTRGEGQLRLAAHEIADYIYQKLIGDRGVFATRLSYVSKVGRRYQL
EEECCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEE
QISDSDGANPQVALTSNEPIISPAWSPDGTKVAYVSFESRKPVVYVHDLISGRRAVISNQ
EECCCCCCCCEEEEECCCCEECCCCCCCCCEEEEEEECCCCCEEEEEEHHCCCEEEEECC
KGNNSAPAWSPDGRRVAVALSRDGNTQIYQVNADGSGLRRLTRSSAIDTEPSFSPDGRSI
CCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCEE
YFTSDRGGAPQIYRMPTEGEDAGSAQRVTFKGGYNTSPRVSPDGKLLAYISRVGGAFKLY
EEECCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCEEEEE
VQDLSNGDVTGLTDTSYDESPSFAANGKFILYATRVGGRSVLAAVSTDGRTRQILSLQAG
EEECCCCCEECCCCCCCCCCCCEECCCEEEEEEEECCCCEEEEEECCCCCCEEEEEECCC
TVREPAWGPFMQ
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852