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The map label for this gene is hemL [H]

Identifier: 17545385

GI number: 17545385

Start: 711010

End: 712311

Strand: Reverse

Name: hemL [H]

Synonym: RSc0666

Alternate gene names: 17545385

Gene position: 712311-711010 (Counterclockwise)

Preceding gene: 17545400

Following gene: 17545383

Centisome position: 19.17

GC content: 69.35

Gene sequence:

>1302_bases
ATGTCCAGCCCGTCCCGTAGTGTCTCCCTGTTTGAACGCGCCCAGAAAACCATCCCCGGCGGCGTGAATTCGCCGGTGCG
GGCGTTCCGCTCGGTCGGCGGCACGCCGCGTTTCATTGCGAAGGCGGCCGGCCCCTACCTGTGGGATGCCGATGGCACCC
GCCTCATTGACTACGTCGGCTCCTGGGGCCCGATGATCGTCGGCCACGCGCACCCCGAGGTGGTGCGCGCCGTCCAGCAG
GTCGCGGCCGACAGCTTTTCGTTCGGCGCGCCCACCGAGGCCGAGGTGGTAATGGCCGAGACCCTCTGCGAGCTGGTGCC
CTCCATCGAGCAGGTCCGGCTGGTGTCGTCCGGAACCGAAGCGACGATGAGCGCCCTGCGCCTAGCACGCGGCTTCACCG
GCCGCGACCTCATCGTGAAATTCGAGGGCTGCTACCACGGACATGCCGACAGCCTGCTGGTCAAGGCCGGCTCGGGCCTG
CTGACCTTCGCGGATACCACGCAGAACGCGCCCTCCTCGGCCGGCGTGCCGGAAGATGTGGTCAAGCACACCATGGTCCT
GCCGTACAACGATGCCGATGCCCTGCGCGAGGCCTTTGCCCGCCACGGCAAGGAGATCGCCGCGGTCATCGTCGAGCCGG
TGGCCGGCAACATGAACCTGGTGCGCGCCACGGCCGCGTTCCTGCAGGCCATGCGCGCGCTGTGCACCGAGCACGGCGCC
GTGCTGATCTTCGACGAAGTGATGACTGGCTTCCGCGTGGCGCTGGGTTGCGCGCAGGCGCTGTACGGCATCACCCCGGA
CCTGACCTGCCTGGGCAAGGTCATCGGCGGCGGCATGCCGGCGGCGGCCTTCGGCGGCCGGCGTGACATCATGGGCTTCC
TCGCGCCGCTGGGCAGCGTGTACCAGGCCGGCACCCTGTCGGGCAATCCGCTGGCGGTGGCCGCCGGCGTGACCACGCTG
CGGCTGATCGCCGCGGACGGCTTCCACGACCGCCTGGCCGCGCAAACGCGCAAGCTGGTCGACGGGCTGGCCGGGATCGC
GCGCGATGCCGGCGTCCCGTTCGCGGCGGACAGCGTGGGCGGCATGTTCGGCCTCTACTTCCGCGAAGGCGTGCCGACCA
GCTTTGCCGAAGTCACGCAGAGCGACGTGGGGCGCTTCAATGCGTTCTTCCACGCGATGCTGGCCGAAGGGGTCTACCTG
GCGCCGTCGGCCTTCGAGGCGGGGTTCGTGTCGTCGATGCATGATGATGCTGTGCTGGAAGCGACGTTCGAAGCGGCGCG
GCGGGCGTTCAAGGCTGTTTGA

Upstream 100 bases:

>100_bases
AGCGGCCAACGCGCGGCTTCTGCGGCAAACCGGCCAAGAACTCCAACCAACCGTGTCCGCGCGCAGGTCTGCGCGGCGCG
CCTTCCCGCCACGATCGTCC

Downstream 100 bases:

>100_bases
GTTTTTTGGGGTGTTGGGGGGCCGTCCCGTGTTCCGGTCCCGGCAGGAGACGGAACGCCGGGACACGGCACCGGAACAAG
GCCTGTCCCGGCCCCCAAGC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSSPSRSVSLFERAQKTIPGGVNSPVRAFRSVGGTPRFIAKAAGPYLWDADGTRLIDYVGSWGPMIVGHAHPEVVRAVQQ
VAADSFSFGAPTEAEVVMAETLCELVPSIEQVRLVSSGTEATMSALRLARGFTGRDLIVKFEGCYHGHADSLLVKAGSGL
LTFADTTQNAPSSAGVPEDVVKHTMVLPYNDADALREAFARHGKEIAAVIVEPVAGNMNLVRATAAFLQAMRALCTEHGA
VLIFDEVMTGFRVALGCAQALYGITPDLTCLGKVIGGGMPAAAFGGRRDIMGFLAPLGSVYQAGTLSGNPLAVAAGVTTL
RLIAADGFHDRLAAQTRKLVDGLAGIARDAGVPFAADSVGGMFGLYFREGVPTSFAEVTQSDVGRFNAFFHAMLAEGVYL
APSAFEAGFVSSMHDDAVLEATFEAARRAFKAV

Sequences:

>Translated_433_residues
MSSPSRSVSLFERAQKTIPGGVNSPVRAFRSVGGTPRFIAKAAGPYLWDADGTRLIDYVGSWGPMIVGHAHPEVVRAVQQ
VAADSFSFGAPTEAEVVMAETLCELVPSIEQVRLVSSGTEATMSALRLARGFTGRDLIVKFEGCYHGHADSLLVKAGSGL
LTFADTTQNAPSSAGVPEDVVKHTMVLPYNDADALREAFARHGKEIAAVIVEPVAGNMNLVRATAAFLQAMRALCTEHGA
VLIFDEVMTGFRVALGCAQALYGITPDLTCLGKVIGGGMPAAAFGGRRDIMGFLAPLGSVYQAGTLSGNPLAVAAGVTTL
RLIAADGFHDRLAAQTRKLVDGLAGIARDAGVPFAADSVGGMFGLYFREGVPTSFAEVTQSDVGRFNAFFHAMLAEGVYL
APSAFEAGFVSSMHDDAVLEATFEAARRAFKAV
>Mature_432_residues
SSPSRSVSLFERAQKTIPGGVNSPVRAFRSVGGTPRFIAKAAGPYLWDADGTRLIDYVGSWGPMIVGHAHPEVVRAVQQV
AADSFSFGAPTEAEVVMAETLCELVPSIEQVRLVSSGTEATMSALRLARGFTGRDLIVKFEGCYHGHADSLLVKAGSGLL
TFADTTQNAPSSAGVPEDVVKHTMVLPYNDADALREAFARHGKEIAAVIVEPVAGNMNLVRATAAFLQAMRALCTEHGAV
LIFDEVMTGFRVALGCAQALYGITPDLTCLGKVIGGGMPAAAFGGRRDIMGFLAPLGSVYQAGTLSGNPLAVAAGVTTLR
LIAADGFHDRLAAQTRKLVDGLAGIARDAGVPFAADSVGGMFGLYFREGVPTSFAEVTQSDVGRFNAFFHAMLAEGVYLA
PSAFEAGFVSSMHDDAVLEATFEAARRAFKAV

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=342, Percent_Identity=27.4853801169591, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI37574042, Length=307, Percent_Identity=27.6872964169381, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI24119277, Length=316, Percent_Identity=26.8987341772152, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI226442705, Length=307, Percent_Identity=26.3843648208469, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI226442709, Length=276, Percent_Identity=27.8985507246377, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI284507298, Length=183, Percent_Identity=30.0546448087432, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1786349, Length=427, Percent_Identity=59.7189695550351, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1789016, Length=362, Percent_Identity=31.4917127071823, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1787560, Length=428, Percent_Identity=29.6728971962617, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788044, Length=299, Percent_Identity=34.1137123745819, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789759, Length=321, Percent_Identity=29.595015576324, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI145693181, Length=316, Percent_Identity=31.0126582278481, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786991, Length=338, Percent_Identity=30.4733727810651, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25144271, Length=332, Percent_Identity=24.6987951807229, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25144274, Length=229, Percent_Identity=24.8908296943231, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=317, Percent_Identity=29.0220820189274, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6324432, Length=348, Percent_Identity=25, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356575, Length=321, Percent_Identity=29.595015576324, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21357415, Length=319, Percent_Identity=26.3322884012539, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 45379; Mature: 45248

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPSRSVSLFERAQKTIPGGVNSPVRAFRSVGGTPRFIAKAAGPYLWDADGTRLIDYVG
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHCCCCEECCCCCHHHHHHC
SWGPMIVGHAHPEVVRAVQQVAADSFSFGAPTEAEVVMAETLCELVPSIEQVRLVSSGTE
CCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCHH
ATMSALRLARGFTGRDLIVKFEGCYHGHADSLLVKAGSGLLTFADTTQNAPSSAGVPEDV
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCHHH
VKHTMVLPYNDADALREAFARHGKEIAAVIVEPVAGNMNLVRATAAFLQAMRALCTEHGA
HHCEEEECCCCHHHHHHHHHHCCHHEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VLIFDEVMTGFRVALGCAQALYGITPDLTCLGKVIGGGMPAAAFGGRRDIMGFLAPLGSV
EEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHH
YQAGTLSGNPLAVAAGVTTLRLIAADGFHDRLAAQTRKLVDGLAGIARDAGVPFAADSVG
HHCCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
GMFGLYFREGVPTSFAEVTQSDVGRFNAFFHAMLAEGVYLAPSAFEAGFVSSMHDDAVLE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHCCCHHHHH
ATFEAARRAFKAV
HHHHHHHHHHHCC
>Mature Secondary Structure 
SSPSRSVSLFERAQKTIPGGVNSPVRAFRSVGGTPRFIAKAAGPYLWDADGTRLIDYVG
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHCCCCEECCCCCHHHHHHC
SWGPMIVGHAHPEVVRAVQQVAADSFSFGAPTEAEVVMAETLCELVPSIEQVRLVSSGTE
CCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCHH
ATMSALRLARGFTGRDLIVKFEGCYHGHADSLLVKAGSGLLTFADTTQNAPSSAGVPEDV
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCHHH
VKHTMVLPYNDADALREAFARHGKEIAAVIVEPVAGNMNLVRATAAFLQAMRALCTEHGA
HHCEEEECCCCHHHHHHHHHHCCHHEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VLIFDEVMTGFRVALGCAQALYGITPDLTCLGKVIGGGMPAAAFGGRRDIMGFLAPLGSV
EEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHH
YQAGTLSGNPLAVAAGVTTLRLIAADGFHDRLAAQTRKLVDGLAGIARDAGVPFAADSVG
HHCCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
GMFGLYFREGVPTSFAEVTQSDVGRFNAFFHAMLAEGVYLAPSAFEAGFVSSMHDDAVLE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHCCCHHHHH
ATFEAARRAFKAV
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA