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The map label for this gene is gloA [H]

Identifier: 17545239

GI number: 17545239

Start: 557915

End: 558322

Strand: Reverse

Name: gloA [H]

Synonym: RSc0520

Alternate gene names: 17545239

Gene position: 558322-557915 (Counterclockwise)

Preceding gene: 17545240

Following gene: 17545238

Centisome position: 15.02

GC content: 64.22

Gene sequence:

>408_bases
ATGCGAATGCTCCACACCATGCTGCGCGTCGGCGACCTGCAACGTTCCATCGACTTCTACACCAAGGTGCTCGGCATGCA
CCTGCTGCGCACCAGCGACAACCCCGAATACAAGTACTCGCTGGCCTTCGTCGGCTACGGCCCGGAAACCGGCAACACGG
TGATCGAGTTGACCTACAACTACGGCGTCAGCGCATACGAGCTGGGCACGGCATTCGGCCATCTCGCCATCGAGGTCGAT
AATGCGGCGCAGGCGTGCGAGCAGATCCGCGCGGCGGGCGGCAAGGTCACGCGCGAGGCCGGCCCGGTCAAGGGCGGCAG
CACCATCATCGCGTTCGTGGAAGACCCGGACGGCTACAAGATCGAACTGATCCAGGCCCGCTCGATGCCCGATGGCAACC
GCCACTGA

Upstream 100 bases:

>100_bases
ATGCACCACCAAGACAAAAACCAACCGCCCCCCACCAGCACATTCCCCCACACCCTCCTACAATACCGGCTCCCCGCAGT
CCCCCAAGGAAGGAAATCCC

Downstream 100 bases:

>100_bases
CCCGGCGCGCCGGCCGCTGGACGGCACCGCCATCGGCCTGATGGTGGTGCTGTGCCTGTGCTGGGGCCTGCAGCAGGTGG
CCATCAAGGTGGCCGTGCAC

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MRMLHTMLRVGDLQRSIDFYTKVLGMHLLRTSDNPEYKYSLAFVGYGPETGNTVIELTYNYGVSAYELGTAFGHLAIEVD
NAAQACEQIRAAGGKVTREAGPVKGGSTIIAFVEDPDGYKIELIQARSMPDGNRH

Sequences:

>Translated_135_residues
MRMLHTMLRVGDLQRSIDFYTKVLGMHLLRTSDNPEYKYSLAFVGYGPETGNTVIELTYNYGVSAYELGTAFGHLAIEVD
NAAQACEQIRAAGGKVTREAGPVKGGSTIIAFVEDPDGYKIELIQARSMPDGNRH
>Mature_135_residues
MRMLHTMLRVGDLQRSIDFYTKVLGMHLLRTSDNPEYKYSLAFVGYGPETGNTVIELTYNYGVSAYELGTAFGHLAIEVD
NAAQACEQIRAAGGKVTREAGPVKGGSTIIAFVEDPDGYKIELIQARSMPDGNRH

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=153, Percent_Identity=34.640522875817, Blast_Score=88, Evalue=2e-18,
Organism=Homo sapiens, GI217330598, Length=143, Percent_Identity=35.6643356643357, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1787940, Length=132, Percent_Identity=79.5454545454545, Blast_Score=223, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17552228, Length=140, Percent_Identity=30.7142857142857, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=38.1294964028777, Blast_Score=94, Evalue=9e-21,
Organism=Drosophila melanogaster, GI20129743, Length=149, Percent_Identity=35.5704697986577, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920404, Length=143, Percent_Identity=37.7622377622378, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14830; Mature: 14830

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMLHTMLRVGDLQRSIDFYTKVLGMHLLRTSDNPEYKYSLAFVGYGPETGNTVIELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEEEEECCCCCCEEEEEEEC
YGVSAYELGTAFGHLAIEVDNAAQACEQIRAAGGKVTREAGPVKGGSTIIAFVEDPDGYK
CCCCEEECCCCEEEEEEEECCHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCE
IELIQARSMPDGNRH
EEEEEECCCCCCCCC
>Mature Secondary Structure
MRMLHTMLRVGDLQRSIDFYTKVLGMHLLRTSDNPEYKYSLAFVGYGPETGNTVIELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEEEEECCCCCCEEEEEEEC
YGVSAYELGTAFGHLAIEVDNAAQACEQIRAAGGKVTREAGPVKGGSTIIAFVEDPDGYK
CCCCEEECCCCEEEEEEEECCHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCE
IELIQARSMPDGNRH
EEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]