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The map label for this gene is ksgA

Identifier: 17545237

GI number: 17545237

Start: 556194

End: 557039

Strand: Direct

Name: ksgA

Synonym: RSc0518

Alternate gene names: 17545237

Gene position: 556194-557039 (Clockwise)

Preceding gene: 17545236

Following gene: 17545258

Centisome position: 14.97

GC content: 69.27

Gene sequence:

>846_bases
ATGGCGCGATCCACTTCCGGCGTTCACCAGGGGCACCAGGCCCGCAAGCGTTTCGGCCAGAACTTCCTGGTCGATGACGG
CGTGATCCACGCCATCGTGGCCGCCATCGACCCGCAGCCGGACGACGTGCTGGTCGAGATCGGCCCGGGCCTCGGCGCGC
TGACCGTGCCGCTGATGGAGCGCGTGCCCACGCTGCAGGTCGTCGAGCTCGATCGCGACCTGGTGGCGCGCCTGCAGCGG
CGCTTCGGCGACAAGCTGATCGTCCACGCGGGCGATGCGCTGGCGTTCGACTTCGGTACGCTGCATGTCCCCGGGCGGTC
GCTGCGCATCGTCGGCAACCTGCCGTACAACATCTCCAGCCCGCTGCTGTTCCACTTGAGCGCGTTTGCCGATCGCGTCC
GCGACCAGCACTTCATGCTGCAGAAGGAGGTGGTCGACCGCATGGTGGCCGCGCCGGGCAGCAAGGCCTTCAGCCGCCTG
TCGGTGATGCTGCAGGTGCGCTACTACATGGAGCTGGTGCTGGAGGTGCCGCCGGGCTCGTTCAATCCGCCGCCCAAGGT
GGATTCGGCGGTGGTGCGGATGATCCCCTGGCCGGCCGACAAATCGCCGTATGCCCCGGTCGACATGCGGGCGCTGGGCA
CGGTGGTGACGCTGGCGTTCTCGCAGCGGCGCAAGGTGCTGCGCAACACGCTGGGATCGCTGCGCGAGGTGATCGACTTC
GACGCGCTCGGGTTCGACCTGGGCCGCCGCGCGGAGGAAGTCCCGGTGGCCGATTTCGTGGCGGTGGCGAACGCGCTGCC
GGCCGAGCGCATCGCCGGCGGCGCGCCCGACCTCAACGAAGATTGA

Upstream 100 bases:

>100_bases
GGAGGCGGGCAGCCTGCTCGAGGCGATCGATACCGCTGTCGAGATGGCCCGCCACGCGGCGCATTCCTGATTCCATTTCT
TCCGTTTTCCGAGCTTCGCT

Downstream 100 bases:

>100_bases
CGAGCAGGATGCCCGACAGCACCAGCGCCGCGGCCAGCGCGAAGCGCAGGCCGACGGGGTCGTGCAGCAGCAGCACGGCG
AACGCCACGCCGAACATCGG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MARSTSGVHQGHQARKRFGQNFLVDDGVIHAIVAAIDPQPDDVLVEIGPGLGALTVPLMERVPTLQVVELDRDLVARLQR
RFGDKLIVHAGDALAFDFGTLHVPGRSLRIVGNLPYNISSPLLFHLSAFADRVRDQHFMLQKEVVDRMVAAPGSKAFSRL
SVMLQVRYYMELVLEVPPGSFNPPPKVDSAVVRMIPWPADKSPYAPVDMRALGTVVTLAFSQRRKVLRNTLGSLREVIDF
DALGFDLGRRAEEVPVADFVAVANALPAERIAGGAPDLNED

Sequences:

>Translated_281_residues
MARSTSGVHQGHQARKRFGQNFLVDDGVIHAIVAAIDPQPDDVLVEIGPGLGALTVPLMERVPTLQVVELDRDLVARLQR
RFGDKLIVHAGDALAFDFGTLHVPGRSLRIVGNLPYNISSPLLFHLSAFADRVRDQHFMLQKEVVDRMVAAPGSKAFSRL
SVMLQVRYYMELVLEVPPGSFNPPPKVDSAVVRMIPWPADKSPYAPVDMRALGTVVTLAFSQRRKVLRNTLGSLREVIDF
DALGFDLGRRAEEVPVADFVAVANALPAERIAGGAPDLNED
>Mature_280_residues
ARSTSGVHQGHQARKRFGQNFLVDDGVIHAIVAAIDPQPDDVLVEIGPGLGALTVPLMERVPTLQVVELDRDLVARLQRR
FGDKLIVHAGDALAFDFGTLHVPGRSLRIVGNLPYNISSPLLFHLSAFADRVRDQHFMLQKEVVDRMVAAPGSKAFSRLS
VMLQVRYYMELVLEVPPGSFNPPPKVDSAVVRMIPWPADKSPYAPVDMRALGTVVTLAFSQRRKVLRNTLGSLREVIDFD
ALGFDLGRRAEEVPVADFVAVANALPAERIAGGAPDLNED

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=220, Percent_Identity=35, Blast_Score=121, Evalue=6e-28,
Organism=Homo sapiens, GI156415992, Length=247, Percent_Identity=32.7935222672065, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1786236, Length=264, Percent_Identity=55.3030303030303, Blast_Score=275, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI25146882, Length=245, Percent_Identity=35.9183673469388, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI25141369, Length=296, Percent_Identity=26.3513513513513, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324989, Length=210, Percent_Identity=30.4761904761905, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21358017, Length=250, Percent_Identity=33.2, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI21357273, Length=267, Percent_Identity=32.5842696629214, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_RALSO (Q8Y219)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518639.1
- ProteinModelPortal:   Q8Y219
- SMR:   Q8Y219
- GeneID:   1219322
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0518
- NMPDR:   fig|267608.1.peg.518
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- BioCyc:   RSOL267608:RSC0518-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30784; Mature: 30653

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 21-21 BINDING 23-23 BINDING 48-48 BINDING 69-69 BINDING 92-92 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSTSGVHQGHQARKRFGQNFLVDDGVIHAIVAAIDPQPDDVLVEIGPGLGALTVPLME
CCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHH
RVPTLQVVELDRDLVARLQRRFGDKLIVHAGDALAFDFGTLHVPGRSLRIVGNLPYNISS
CCCCEEEEECCHHHHHHHHHHCCCEEEEEECCEEEEECCEEECCCCEEEEEECCCCCCCC
PLLFHLSAFADRVRDQHFMLQKEVVDRMVAAPGSKAFSRLSVMLQVRYYMELVLEVPPGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
FNPPPKVDSAVVRMIPWPADKSPYAPVDMRALGTVVTLAFSQRRKVLRNTLGSLREVIDF
CCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALGFDLGRRAEEVPVADFVAVANALPAERIAGGAPDLNED
HHHCHHHCCCHHHCCHHHHHHHHHCCCHHHHCCCCCCCCCC
>Mature Secondary Structure 
ARSTSGVHQGHQARKRFGQNFLVDDGVIHAIVAAIDPQPDDVLVEIGPGLGALTVPLME
CCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHH
RVPTLQVVELDRDLVARLQRRFGDKLIVHAGDALAFDFGTLHVPGRSLRIVGNLPYNISS
CCCCEEEEECCHHHHHHHHHHCCCEEEEEECCEEEEECCEEECCCCEEEEEECCCCCCCC
PLLFHLSAFADRVRDQHFMLQKEVVDRMVAAPGSKAFSRLSVMLQVRYYMELVLEVPPGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
FNPPPKVDSAVVRMIPWPADKSPYAPVDMRALGTVVTLAFSQRRKVLRNTLGSLREVIDF
CCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALGFDLGRRAEEVPVADFVAVANALPAERIAGGAPDLNED
HHHCHHHCCCHHHCCHHHHHHHHHCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852