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The map label for this gene is tyrS

Identifier: 17545215

GI number: 17545215

Start: 531680

End: 532858

Strand: Direct

Name: tyrS

Synonym: RSc0496

Alternate gene names: 17545215

Gene position: 531680-532858 (Clockwise)

Preceding gene: 17545213

Following gene: 17545216

Centisome position: 14.31

GC content: 65.82

Gene sequence:

>1179_bases
ATGGAGGTCACGCAGCGCGGCTGCGACGAACTGCTGATCGCTGCGGAGTGGGAGCAGAAGCTGGCCCGCAGCGCCGCCAC
CGGCGTGCCGCTGCGCATCAAGCTGGGCCTGGACCCGACCGCGCCAGACATCCACATCGGCCACACCGTGGTGCTCAACA
AGATGCGCCAGCTGCAGGACCTGGGGCACCAGGTGATCTTCCTGATCGGCGATTTCACCTCGACCATCGGCGATCCGTCG
GGCCGCAACGCCACGCGCCCGCCGCTCACGCGCGAGCAGATCGAGGCCAACGCGCAGACCTACTACCGCCAGGCCAGCAT
GGTGCTGGACCCGAGCAAGACCGAGATCCGCTACAACAGCGAGTGGTGCGATCCGCTGGGCGCGCGCGGCATCATCCAGC
TCGCCGCCAAGTACACCGTGGCCCGCATGATGGAGCGCGACGACTTCACCAAGCGCTACAAGGCGGGCGTGCCGATCTCG
GTGCACGAATTCCTCTACCCGCTGATGCAGGGCTATGACTCGGTGGCGCTGAAGTCCGACCTGGAGCTCGGTGGGACCGA
CCAGAAATTCAACCTGCTGGTCGGCCGTGAGCTGCAGAAGGAATACGGCCAGGAGCCGCAGTGCATCTTGACGATGCCGC
TGCTGGTCGGCCTGGACGGCGTGGAGAAGATGTCCAAGTCCAAGGGCAACTACGTCGGCATCAGCGAGGCGCCCAACGAG
ATGTTCGGCAAGCTGATGAGCATCTCCGACGACCTGATGTGGACGTACTACACGCTGCTGTCGTTCCGTCCGCTGGCCGA
GATCGACCTGATGAAGCAGGAAGTGACGCTGGGCCGCAACCCGCGCGACTGCAAGGTGCTGCTGGCGCAAGAAATCATCG
CGCGCTTCCACAGCCAGGCCGATGCCGAGCGCGCGCTGGAGGACTTCAACCACCGCGCGCGCGGCGGCGTGCCGGACGAC
ATTCCGCAGATCGAGCTGTCGGGCGCGCCGATCGGCATCGCGCAACTGCTCAAGCAGGCCGGCCTGTGCCCGTCCACCTC
GGAAGCCAACCGCAATATCGAGCAGGGCGGGGTGAAGATCGACGGCACCGTCATCAGCGACAAGGGGCTGAAGGTCGAGG
CCGGCACCTTTGTGATGCAGGTGGGCAAGCGGCGCTTTGCGCGCGTGGTGCTGTCGTGA

Upstream 100 bases:

>100_bases
ATTTGCCATGACCGTTTCCGACGCCTCCGCTGCTCCCGCTCCCGAACACAACACGCCGGGCAAACCGAAGTACCCGGTCA
CGCCGGAGGTGCTGCATGCG

Downstream 100 bases:

>100_bases
TCGGTCTGATCCAGCGCGTCTCGCAGGCGGCGGTGCGGGTCGATGGCCGCGTGGTCGGCGAGATCGGGCCGGGCCTGCTG
GCGCTGGTGTGCGCCGAGCG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MEVTQRGCDELLIAAEWEQKLARSAATGVPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQDLGHQVIFLIGDFTSTIGDPS
GRNATRPPLTREQIEANAQTYYRQASMVLDPSKTEIRYNSEWCDPLGARGIIQLAAKYTVARMMERDDFTKRYKAGVPIS
VHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQEPQCILTMPLLVGLDGVEKMSKSKGNYVGISEAPNE
MFGKLMSISDDLMWTYYTLLSFRPLAEIDLMKQEVTLGRNPRDCKVLLAQEIIARFHSQADAERALEDFNHRARGGVPDD
IPQIELSGAPIGIAQLLKQAGLCPSTSEANRNIEQGGVKIDGTVISDKGLKVEAGTFVMQVGKRRFARVVLS

Sequences:

>Translated_392_residues
MEVTQRGCDELLIAAEWEQKLARSAATGVPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQDLGHQVIFLIGDFTSTIGDPS
GRNATRPPLTREQIEANAQTYYRQASMVLDPSKTEIRYNSEWCDPLGARGIIQLAAKYTVARMMERDDFTKRYKAGVPIS
VHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQEPQCILTMPLLVGLDGVEKMSKSKGNYVGISEAPNE
MFGKLMSISDDLMWTYYTLLSFRPLAEIDLMKQEVTLGRNPRDCKVLLAQEIIARFHSQADAERALEDFNHRARGGVPDD
IPQIELSGAPIGIAQLLKQAGLCPSTSEANRNIEQGGVKIDGTVISDKGLKVEAGTFVMQVGKRRFARVVLS
>Mature_392_residues
MEVTQRGCDELLIAAEWEQKLARSAATGVPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQDLGHQVIFLIGDFTSTIGDPS
GRNATRPPLTREQIEANAQTYYRQASMVLDPSKTEIRYNSEWCDPLGARGIIQLAAKYTVARMMERDDFTKRYKAGVPIS
VHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQEPQCILTMPLLVGLDGVEKMSKSKGNYVGISEAPNE
MFGKLMSISDDLMWTYYTLLSFRPLAEIDLMKQEVTLGRNPRDCKVLLAQEIIARFHSQADAERALEDFNHRARGGVPDD
IPQIELSGAPIGIAQLLKQAGLCPSTSEANRNIEQGGVKIDGTVISDKGLKVEAGTFVMQVGKRRFARVVLS

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=408, Percent_Identity=27.2058823529412, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1787925, Length=380, Percent_Identity=27.1052631578947, Blast_Score=121, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI25145802, Length=261, Percent_Identity=27.2030651340996, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6325160, Length=433, Percent_Identity=23.5565819861432, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20130383, Length=401, Percent_Identity=25.9351620947631, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_RALSO (Q8Y240)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518617.1
- ProteinModelPortal:   Q8Y240
- SMR:   Q8Y240
- GeneID:   1219300
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0496
- NMPDR:   fig|267608.1.peg.496
- HOGENOM:   HBG288125
- OMA:   YVVQVGK
- ProtClustDB:   PRK05912
- BioCyc:   RSOL267608:RSC0496-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR000253
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 43497; Mature: 43497

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4; PS50006 FHA_DOMAIN

Important sites: BINDING 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVTQRGCDELLIAAEWEQKLARSAATGVPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQD
CCCCHHCHHHHEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCHHHHHHHHHHHHH
LGHQVIFLIGDFTSTIGDPSGRNATRPPLTREQIEANAQTYYRQASMVLDPSKTEIRYNS
CCCEEEEEEECHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCEEEECC
EWCDPLGARGIIQLAAKYTVARMMERDDFTKRYKAGVPISVHEFLYPLMQGYDSVALKSD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCEEECCC
LELGGTDQKFNLLVGRELQKEYGQEPQCILTMPLLVGLDGVEKMSKSKGNYVGISEAPNE
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCHHHHHHHCCCCEEECCCCCHH
MFGKLMSISDDLMWTYYTLLSFRPLAEIDLMKQEVTLGRNPRDCKVLLAQEIIARFHSQA
HHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
DAERALEDFNHRARGGVPDDIPQIELSGAPIGIAQLLKQAGLCPSTSEANRNIEQGGVKI
HHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCCCHHCCCHHHCCEEE
DGTVISDKGLKVEAGTFVMQVGKRRFARVVLS
EEEEECCCCCEEEHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEVTQRGCDELLIAAEWEQKLARSAATGVPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQD
CCCCHHCHHHHEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCHHHHHHHHHHHHH
LGHQVIFLIGDFTSTIGDPSGRNATRPPLTREQIEANAQTYYRQASMVLDPSKTEIRYNS
CCCEEEEEEECHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCEEEECC
EWCDPLGARGIIQLAAKYTVARMMERDDFTKRYKAGVPISVHEFLYPLMQGYDSVALKSD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCEEECCC
LELGGTDQKFNLLVGRELQKEYGQEPQCILTMPLLVGLDGVEKMSKSKGNYVGISEAPNE
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCHHHHHHHCCCCEEECCCCCHH
MFGKLMSISDDLMWTYYTLLSFRPLAEIDLMKQEVTLGRNPRDCKVLLAQEIIARFHSQA
HHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
DAERALEDFNHRARGGVPDDIPQIELSGAPIGIAQLLKQAGLCPSTSEANRNIEQGGVKI
HHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCCCHHCCCHHHCCEEE
DGTVISDKGLKVEAGTFVMQVGKRRFARVVLS
EEEEECCCCCEEEHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852