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The map label for this gene is queF

Identifier: 17545167

GI number: 17545167

Start: 476091

End: 476924

Strand: Reverse

Name: queF

Synonym: RSc0448

Alternate gene names: 17545167

Gene position: 476924-476091 (Counterclockwise)

Preceding gene: 17545168

Following gene: 17545166

Centisome position: 12.83

GC content: 64.51

Gene sequence:

>834_bases
ATGAGCCATCCCGAACACTCACCGCTGGGCAAGGCCTCGGCCTACAAGACGCAGTACGACCCGAGCCTGCTGTTCCCCAT
TCCGCGCCAGGCCAAACGTGACGAGATCGGCCTGGCCGCCGGCAGCGCGCTGCCCTTCTTCGGCATCGATCTGTGGAACC
TGTACGAGCTGTCGTGGCTGAACCTCAAGGGCAAGCCACAGGTGGCGATCGGCACCGTGATCGTGCCGGCGGATTCACCC
AACATCGTCGAATCGAAGTCGTTCAAGCTGTACCTGAACACGTTCAACCAGACCAAGGTGGCCTCGAGCGAGGCGCTGCA
GCAGCTGATCCATCACGACCTGTCGGAGGCGTGCGGCGCGCCGGTGCAGGTGCGCATCGTGCCGCAGGAGGAATTTGCCC
GGCAGAAGATGGGCGAGCTCGCAGGCCTGTCGCTCGACCGGCTGGACGTCGAAACCGACGTCTACCAGCCCACGCCCGGG
CTGCTGCATGCCGACCAGGATGAGAGTCCGGTGGAGGAAGTGCTGGTGTCGCACCTGCTCAAGTCCAACTGCCTGGTGAC
GGGCCAGCCGGACTGGGGCAGCGTGCAGATCCGCTACGTGGGCGCGCCGATCAACCAGGAAGGGCTGCTCAAGTACCTGA
TCTCGTTCCGCGAGCACAACGAATTCCACGAGCAGTGCGTCGAGCGCATCTTCATGGACATCCAGCGCCAGTGCCGGCCG
GTCAAGCTGGCGGTGTACGCGCGCTATACGCGGCGCGGCGGGCTGGATATCAACCCGTTCCGGACCAACTTCAACACGCC
CTGGCCAGACAACCTGCGCAACGCGCGCCAATAG

Upstream 100 bases:

>100_bases
TTCCTCGCCACGCTCGGCTACACGTTCTGGGACGAAAGCGAGAACCCGGTCTACGGACTGTTCCTGCGCTGATCCCATCC
GTTGCCGTGGAGTCTTCCGC

Downstream 100 bases:

>100_bases
GCCGCGCGTGCCGGCCGCACGCAGACCAGGGCGCCTTCGGGCGCCCTTTTTTCTTGCCCGCGCGTCGACCGAACGACATA
CGCGGCATCGTCCGTTTTGG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSHPEHSPLGKASAYKTQYDPSLLFPIPRQAKRDEIGLAAGSALPFFGIDLWNLYELSWLNLKGKPQVAIGTVIVPADSP
NIVESKSFKLYLNTFNQTKVASSEALQQLIHHDLSEACGAPVQVRIVPQEEFARQKMGELAGLSLDRLDVETDVYQPTPG
LLHADQDESPVEEVLVSHLLKSNCLVTGQPDWGSVQIRYVGAPINQEGLLKYLISFREHNEFHEQCVERIFMDIQRQCRP
VKLAVYARYTRRGGLDINPFRTNFNTPWPDNLRNARQ

Sequences:

>Translated_277_residues
MSHPEHSPLGKASAYKTQYDPSLLFPIPRQAKRDEIGLAAGSALPFFGIDLWNLYELSWLNLKGKPQVAIGTVIVPADSP
NIVESKSFKLYLNTFNQTKVASSEALQQLIHHDLSEACGAPVQVRIVPQEEFARQKMGELAGLSLDRLDVETDVYQPTPG
LLHADQDESPVEEVLVSHLLKSNCLVTGQPDWGSVQIRYVGAPINQEGLLKYLISFREHNEFHEQCVERIFMDIQRQCRP
VKLAVYARYTRRGGLDINPFRTNFNTPWPDNLRNARQ
>Mature_276_residues
SHPEHSPLGKASAYKTQYDPSLLFPIPRQAKRDEIGLAAGSALPFFGIDLWNLYELSWLNLKGKPQVAIGTVIVPADSPN
IVESKSFKLYLNTFNQTKVASSEALQQLIHHDLSEACGAPVQVRIVPQEEFARQKMGELAGLSLDRLDVETDVYQPTPGL
LHADQDESPVEEVLVSHLLKSNCLVTGQPDWGSVQIRYVGAPINQEGLLKYLISFREHNEFHEQCVERIFMDIQRQCRPV
KLAVYARYTRRGGLDINPFRTNFNTPWPDNLRNARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=270, Percent_Identity=53.7037037037037, Blast_Score=285, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_RALSO (Q8Y288)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518569.1
- ProteinModelPortal:   Q8Y288
- SMR:   Q8Y288
- GeneID:   1219252
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0448
- NMPDR:   fig|267608.1.peg.448
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- ProtClustDB:   PRK11792
- BioCyc:   RSOL267608:RSC0448-MONOMER
- BRENDA:   1.7.1.13
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 31276; Mature: 31145

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHPEHSPLGKASAYKTQYDPSLLFPIPRQAKRDEIGLAAGSALPFFGIDLWNLYELSWL
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCCCEECCCHHHEEEEEEE
NLKGKPQVAIGTVIVPADSPNIVESKSFKLYLNTFNQTKVASSEALQQLIHHDLSEACGA
CCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
PVQVRIVPQEEFARQKMGELAGLSLDRLDVETDVYQPTPGLLHADQDESPVEEVLVSHLL
CEEEEEECHHHHHHHHHHHHHCCCHHHEECCCCCCCCCCCCEECCCCCCHHHHHHHHHHH
KSNCLVTGQPDWGSVQIRYVGAPINQEGLLKYLISFREHNEFHEQCVERIFMDIQRQCRP
HCCCEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCC
VKLAVYARYTRRGGLDINPFRTNFNTPWPDNLRNARQ
EEEEEEEEEHHCCCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
SHPEHSPLGKASAYKTQYDPSLLFPIPRQAKRDEIGLAAGSALPFFGIDLWNLYELSWL
CCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCCCEECCCHHHEEEEEEE
NLKGKPQVAIGTVIVPADSPNIVESKSFKLYLNTFNQTKVASSEALQQLIHHDLSEACGA
CCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
PVQVRIVPQEEFARQKMGELAGLSLDRLDVETDVYQPTPGLLHADQDESPVEEVLVSHLL
CEEEEEECHHHHHHHHHHHHHCCCHHHEECCCCCCCCCCCCEECCCCCCHHHHHHHHHHH
KSNCLVTGQPDWGSVQIRYVGAPINQEGLLKYLISFREHNEFHEQCVERIFMDIQRQCRP
HCCCEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCC
VKLAVYARYTRRGGLDINPFRTNFNTPWPDNLRNARQ
EEEEEEEEEHHCCCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852