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The map label for this gene is rhtB [C]

Identifier: 17545137

GI number: 17545137

Start: 445016

End: 445636

Strand: Direct

Name: rhtB [C]

Synonym: RSc0418

Alternate gene names: 17545137

Gene position: 445016-445636 (Clockwise)

Preceding gene: 17545136

Following gene: 17545140

Centisome position: 11.97

GC content: 71.66

Gene sequence:

>621_bases
ATGCCGAATGTGCTGCTGTTCCTGCTGTCTTCCGTCGCCATCACGGTGATGCCGGGGCCGGACAACCTGCAGGTGATCGC
GCGTGGCGCGAGCCAGGGCCGCAAGGCCGGGCTGGCGGCGGCGGCCGGGTTTGCCTCGGGCTGCCTGTTCCATACGACGC
TGGCGGCGCTGGGGCTGGCGGCGGTGCTGCAGTCCGCGCCGGCGGCGTTCCAGGCGATCCGCTGGCTGGGCGCGGCCTAC
CTGGTCTGGCTGGGCGTGCAGGCGCTGCGCAGCAAGGGCGGCGTCGGGCCGTCGGTGAGCGCGCAGGCGGCGGCGCCCGA
TCTGTGGCAGGTGTTCCGCCAGAGCGTGCTGGCCAACATGCTCAACCCCAAGGTGACGCTGTTCTTCGTGGTGTTCCTGC
CGCAGTTCGTCGACGCGCAGGCCGGTCATGCGGCGCTGCAGATGCTGCTGCTGGGCGGGGTGTTCATGGCGCAGACCATC
GTGGTCTTCGGCCTGTACGGCTGGTGCGCGGCGGCGCTGGGCGGCTGGATGCGCCGCACGCCGCGCGCGTCGCTGTGGCT
GGATCGCGTGAGCGGCTGCATTTTCATCGGGCTGGGGCTGCGGGTCGCGCTCACCAAGTGA

Upstream 100 bases:

>100_bases
ATGGCCGCCGGCGATGCCCTGGCCGCATCCCTTGGTGTCACCGCAACAAGACGCCGCCGCGCGCCGCGCGGTGGTGTGGC
CTTTTGATGAGAGGACAACG

Downstream 100 bases:

>100_bases
GTCGCGCGCCAGGCAGGAAAGGCGGGCCGCGCTGCCCAGGCGGCTGGTCCGTGGCCGTACCCGCGAGGCGGAAGCGGCCA
CCGCCGCCGGGGCCCGGTCG

Product: transport transmembrane protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MPNVLLFLLSSVAITVMPGPDNLQVIARGASQGRKAGLAAAAGFASGCLFHTTLAALGLAAVLQSAPAAFQAIRWLGAAY
LVWLGVQALRSKGGVGPSVSAQAAAPDLWQVFRQSVLANMLNPKVTLFFVVFLPQFVDAQAGHAALQMLLLGGVFMAQTI
VVFGLYGWCAAALGGWMRRTPRASLWLDRVSGCIFIGLGLRVALTK

Sequences:

>Translated_206_residues
MPNVLLFLLSSVAITVMPGPDNLQVIARGASQGRKAGLAAAAGFASGCLFHTTLAALGLAAVLQSAPAAFQAIRWLGAAY
LVWLGVQALRSKGGVGPSVSAQAAAPDLWQVFRQSVLANMLNPKVTLFFVVFLPQFVDAQAGHAALQMLLLGGVFMAQTI
VVFGLYGWCAAALGGWMRRTPRASLWLDRVSGCIFIGLGLRVALTK
>Mature_205_residues
PNVLLFLLSSVAITVMPGPDNLQVIARGASQGRKAGLAAAAGFASGCLFHTTLAALGLAAVLQSAPAAFQAIRWLGAAYL
VWLGVQALRSKGGVGPSVSAQAAAPDLWQVFRQSVLANMLNPKVTLFFVVFLPQFVDAQAGHAALQMLLLGGVFMAQTIV
VFGLYGWCAAALGGWMRRTPRASLWLDRVSGCIFIGLGLRVALTK

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=193, Percent_Identity=27.4611398963731, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1788099, Length=201, Percent_Identity=27.363184079602, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1786522, Length=124, Percent_Identity=27.4193548387097, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21580; Mature: 21449

Theoretical pI: Translated: 11.45; Mature: 11.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNVLLFLLSSVAITVMPGPDNLQVIARGASQGRKAGLAAAAGFASGCLFHTTLAALGLA
CCHHHHHHHHHHHEEEECCCCCHHHEECCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLQSAPAAFQAIRWLGAAYLVWLGVQALRSKGGVGPSVSAQAAAPDLWQVFRQSVLANM
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHH
LNPKVTLFFVVFLPQFVDAQAGHAALQMLLLGGVFMAQTIVVFGLYGWCAAALGGWMRRT
HCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PRASLWLDRVSGCIFIGLGLRVALTK
CCHHHHHHHHCCCEEEECCCEEEECC
>Mature Secondary Structure 
PNVLLFLLSSVAITVMPGPDNLQVIARGASQGRKAGLAAAAGFASGCLFHTTLAALGLA
CHHHHHHHHHHHEEEECCCCCHHHEECCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLQSAPAAFQAIRWLGAAYLVWLGVQALRSKGGVGPSVSAQAAAPDLWQVFRQSVLANM
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHH
LNPKVTLFFVVFLPQFVDAQAGHAALQMLLLGGVFMAQTIVVFGLYGWCAAALGGWMRRT
HCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PRASLWLDRVSGCIFIGLGLRVALTK
CCHHHHHHHHCCCEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8169201; 10984043 [H]