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The map label for this gene is maiA [H]

Identifier: 17545103

GI number: 17545103

Start: 412642

End: 413292

Strand: Direct

Name: maiA [H]

Synonym: RSc0384

Alternate gene names: 17545103

Gene position: 412642-413292 (Clockwise)

Preceding gene: 17545102

Following gene: 17545104

Centisome position: 11.1

GC content: 68.66

Gene sequence:

>651_bases
ATGTCGATCAAGCTGTACAACTACTTCCGCAGCTCGGCGTCGTTCCGCGTGCGCATCGCGCTGGAGATCAAGGGCCTGCC
GTATGACTATGCGCCGGTGCACCTGCTCAAGGGCGAGCAAGCCGCGCCCGACTTCGTCAAGCTGAACCCGGAAGCGCTGG
TGCCGGTGCTGTGCGACGGCACCGAGGTCCTGAACCAGTCGCTGGCGATCGTCGAGTATCTGGAAGAGACGCATCCCGAG
CCGACGCTGCTGCCGGGTTCGGCGGCCGACCGCGCCCACATCCGCGCCATCGCGCTGGCGATCGCCTGCGAGATCCATCC
GCTGAACAATCCGCGCGTGCTCAAGTACCTCAAGCACACCTTCAACGTGGGCGACGACGCCCGCAATGACTGGTATCGCC
ATTGGGTGCGCCTGGGCTTCGCCGCGCTGGAGACGCGCCTGACGCAGTCGCCGCGCACCGGCGCCTGCTGCGTGGGCGAC
ACGCCGACGCTGGCCGACCTGTGCCTGGTGCCGCAGGTCTTCAACGGCAAGCGCTTCGACGTGGCGGTGGAGGACTACCC
GACGCTCGCCCGCGTCTTCGAGCACTGCATGGCCCAGCCGGCGTTCCAGCGCGCCGCGCCCGCCGCCCAGCCCGACGCCG
CGTCCGCATGA

Upstream 100 bases:

>100_bases
CACCGGCACGCCCGAGGGCGTCGGCCCGGTCTCGATCGGCGACCTGATGGTCGGCCGGATCGACGGCGTGGGCGAGCTGC
ACGTGAAGGTGGTGTGAGCG

Downstream 100 bases:

>100_bases
CGGGGCCCGGCCCGGAGAGCGCGCCGCTGATCGGCGCGGCGCTGTCGGGCTTCGGCCTGGGCGCGAGTCTGATCGTGGCG
ATCGGCGCGCAGAACGCGTA

Product: maleylacetoacetate isomerase (glutathione transferase zeta 1) protein

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSIKLYNYFRSSASFRVRIALEIKGLPYDYAPVHLLKGEQAAPDFVKLNPEALVPVLCDGTEVLNQSLAIVEYLEETHPE
PTLLPGSAADRAHIRAIALAIACEIHPLNNPRVLKYLKHTFNVGDDARNDWYRHWVRLGFAALETRLTQSPRTGACCVGD
TPTLADLCLVPQVFNGKRFDVAVEDYPTLARVFEHCMAQPAFQRAAPAAQPDAASA

Sequences:

>Translated_216_residues
MSIKLYNYFRSSASFRVRIALEIKGLPYDYAPVHLLKGEQAAPDFVKLNPEALVPVLCDGTEVLNQSLAIVEYLEETHPE
PTLLPGSAADRAHIRAIALAIACEIHPLNNPRVLKYLKHTFNVGDDARNDWYRHWVRLGFAALETRLTQSPRTGACCVGD
TPTLADLCLVPQVFNGKRFDVAVEDYPTLARVFEHCMAQPAFQRAAPAAQPDAASA
>Mature_215_residues
SIKLYNYFRSSASFRVRIALEIKGLPYDYAPVHLLKGEQAAPDFVKLNPEALVPVLCDGTEVLNQSLAIVEYLEETHPEP
TLLPGSAADRAHIRAIALAIACEIHPLNNPRVLKYLKHTFNVGDDARNDWYRHWVRLGFAALETRLTQSPRTGACCVGDT
PTLADLCLVPQVFNGKRFDVAVEDYPTLARVFEHCMAQPAFQRAAPAAQPDAASA

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=211, Percent_Identity=45.4976303317536, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI22202622, Length=162, Percent_Identity=42.5925925925926, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI194440732, Length=212, Percent_Identity=33.4905660377358, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17551302, Length=211, Percent_Identity=42.654028436019, Blast_Score=152, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17510461, Length=211, Percent_Identity=40.7582938388626, Blast_Score=143, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17556142, Length=211, Percent_Identity=38.8625592417062, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24645375, Length=210, Percent_Identity=43.3333333333333, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI45553325, Length=210, Percent_Identity=43.3333333333333, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI21355859, Length=210, Percent_Identity=43.3333333333333, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI21355857, Length=211, Percent_Identity=40.7582938388626, Blast_Score=154, Evalue=3e-38,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 23898; Mature: 23766

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKLYNYFRSSASFRVRIALEIKGLPYDYAPVHLLKGEQAAPDFVKLNPEALVPVLCDG
CEEEEEEHHCCCCCEEEEEEEEEECCCCCCCCEEEECCCCCCCCEEEECHHHEEEEEECC
TEVLNQSLAIVEYLEETHPEPTLLPGSAADRAHIRAIALAIACEIHPLNNPRVLKYLKHT
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHH
FNVGDDARNDWYRHWVRLGFAALETRLTQSPRTGACCVGDTPTLADLCLVPQVFNGKRFD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCCEEE
VAVEDYPTLARVFEHCMAQPAFQRAAPAAQPDAASA
EECCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SIKLYNYFRSSASFRVRIALEIKGLPYDYAPVHLLKGEQAAPDFVKLNPEALVPVLCDG
EEEEEEHHCCCCCEEEEEEEEEECCCCCCCCEEEECCCCCCCCEEEECHHHEEEEEECC
TEVLNQSLAIVEYLEETHPEPTLLPGSAADRAHIRAIALAIACEIHPLNNPRVLKYLKHT
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHH
FNVGDDARNDWYRHWVRLGFAALETRLTQSPRTGACCVGDTPTLADLCLVPQVFNGKRFD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCCEEE
VAVEDYPTLARVFEHCMAQPAFQRAAPAAQPDAASA
EECCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]