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The map label for this gene is gshB

Identifier: 17545064

GI number: 17545064

Start: 377965

End: 378939

Strand: Direct

Name: gshB

Synonym: RSc0345

Alternate gene names: 17545064

Gene position: 377965-378939 (Clockwise)

Preceding gene: 17545063

Following gene: 17545065

Centisome position: 10.17

GC content: 68.0

Gene sequence:

>975_bases
ATGCGCATCCTCTTCATCGTCGATCCGCTGTCAACATTCAAGATTTACAAGGATTCCACCTTCGCGATGATGCGCGAGGC
GGCGGCGCGCGGCTATGCCATCTATACCTGCCAGCAGTCGCAGCTGACGCTGTCGGGCAATGTGGTCGAGACGGTCGCCA
CGCCCCTTGCCCTGACCGGCGACGAGCACGACTGGTACCGCTCCGGCGACCCGCGCCTGCTGCCGCTGACCGGCTTCGAC
GCCGTGCTGATGCGCAAGGACCCGCCGTTCGACATGGAATACGTCACCAGCACCTGGCTGCTGGAGATCGCCGAGCGGCA
GGGCGCGCGCGTGTTCAACAAGCCGCAGGCGATCCGCGACCACTCCGAGAAGCTGGCGATCGCGCAGTTCCGCGAGTTCA
CCGCACCGACCATCGTCACGCGCGACGCCAAGCGCCTGCGCGAATTCCACGCCGAGCAGGGCGATGTCATCTTCAAGCCG
CTGGACGGCATGGGCGGGGCCGGCATCTTCCGGGTCGGCGCGGACGGCATGAACCTCGGCTCGGTGATCGAGACGCTGAC
CCACAACGGCACGCGCACGGTCATGGCCCAGCAGTACATCCCGGCGATCCGCGATGGCGACAAGCGCATCCTGCTGATCG
GCGGTTCCCCGGTGCCGCACGCGCTGGCGCGCGTCCCGATGGCCGGCGAAGTGCGCGGCAACCTGGCGGCGGGCGGCACG
GGCCGGGCGCAACTGCTGTCCGAGCGGGATCAGGTGATTGCGCATGCACTGGCGCCGGTGCTGTGGCAGCGTGGCCTGCT
GCTGGTCGGCCTGGACGTCATCGGCGACTACCTGACCGAAGTCAACGTCACCAGCCCGACCTGCTTCCAGGAGATCACCC
AGCAGACCGGCTTCAACGTCGCCGGCATGTTCATCGATGCCCTGGAGCGCGCGGCCGGCAAGGCGAGCGGCGGACTGGGG
CGCAAGCTGGCCTAG

Upstream 100 bases:

>100_bases
GTGGTGGCGCGCCTGGCGCTGCTGGCCGCGTCGCTCGAACTCGAGAAGACCGACCCGAACCCGCTGATCTGATTCCTGTA
ATACGGTCCCTGGAGCCGCC

Downstream 100 bases:

>100_bases
CGGACCCCGCACGCGGTCATCCCCCACCGGAGGGAGGCGGGCCGCATCAGCGCACAATGGCCATAAACGGCCCTGATACA
ATCGGTCTCACACCGATTCG

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MRILFIVDPLSTFKIYKDSTFAMMREAAARGYAIYTCQQSQLTLSGNVVETVATPLALTGDEHDWYRSGDPRLLPLTGFD
AVLMRKDPPFDMEYVTSTWLLEIAERQGARVFNKPQAIRDHSEKLAIAQFREFTAPTIVTRDAKRLREFHAEQGDVIFKP
LDGMGGAGIFRVGADGMNLGSVIETLTHNGTRTVMAQQYIPAIRDGDKRILLIGGSPVPHALARVPMAGEVRGNLAAGGT
GRAQLLSERDQVIAHALAPVLWQRGLLLVGLDVIGDYLTEVNVTSPTCFQEITQQTGFNVAGMFIDALERAAGKASGGLG
RKLA

Sequences:

>Translated_324_residues
MRILFIVDPLSTFKIYKDSTFAMMREAAARGYAIYTCQQSQLTLSGNVVETVATPLALTGDEHDWYRSGDPRLLPLTGFD
AVLMRKDPPFDMEYVTSTWLLEIAERQGARVFNKPQAIRDHSEKLAIAQFREFTAPTIVTRDAKRLREFHAEQGDVIFKP
LDGMGGAGIFRVGADGMNLGSVIETLTHNGTRTVMAQQYIPAIRDGDKRILLIGGSPVPHALARVPMAGEVRGNLAAGGT
GRAQLLSERDQVIAHALAPVLWQRGLLLVGLDVIGDYLTEVNVTSPTCFQEITQQTGFNVAGMFIDALERAAGKASGGLG
RKLA
>Mature_324_residues
MRILFIVDPLSTFKIYKDSTFAMMREAAARGYAIYTCQQSQLTLSGNVVETVATPLALTGDEHDWYRSGDPRLLPLTGFD
AVLMRKDPPFDMEYVTSTWLLEIAERQGARVFNKPQAIRDHSEKLAIAQFREFTAPTIVTRDAKRLREFHAEQGDVIFKP
LDGMGGAGIFRVGADGMNLGSVIETLTHNGTRTVMAQQYIPAIRDGDKRILLIGGSPVPHALARVPMAGEVRGNLAAGGT
GRAQLLSERDQVIAHALAPVLWQRGLLLVGLDVIGDYLTEVNVTSPTCFQEITQQTGFNVAGMFIDALERAAGKASGGLG
RKLA

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1789316, Length=309, Percent_Identity=47.2491909385113, Blast_Score=307, Evalue=5e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSHB_RALSO (P58579)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518466.1
- ProteinModelPortal:   P58579
- SMR:   P58579
- GeneID:   1219148
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0345
- NMPDR:   fig|267608.1.peg.345
- HOGENOM:   HBG624526
- OMA:   WMRKDPP
- ProtClustDB:   PRK05246
- BioCyc:   RSOL267608:RSC0345-MONOMER
- BRENDA:   6.3.2.3
- HAMAP:   MF_00162
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01380

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.3

Molecular weight: Translated: 35330; Mature: 35330

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILFIVDPLSTFKIYKDSTFAMMREAAARGYAIYTCQQSQLTLSGNVVETVATPLALTG
CEEEEEECCCCCEEEECCCHHHHHHHHHHCCEEEEEEECCCEEECCCCHHHHHCCEEEEC
DEHDWYRSGDPRLLPLTGFDAVLMRKDPPFDMEYVTSTWLLEIAERQGARVFNKPQAIRD
CCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
HSEKLAIAQFREFTAPTIVTRDAKRLREFHAEQGDVIFKPLDGMGGAGIFRVGADGMNLG
HHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCCHH
SVIETLTHNGTRTVMAQQYIPAIRDGDKRILLIGGSPVPHALARVPMAGEVRGNLAAGGT
HHHHHHHCCCCCEEHHHHHCCHHCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCEECCCC
GRAQLLSERDQVIAHALAPVLWQRGLLLVGLDVIGDYLTEVNVTSPTCFQEITQQTGFNV
CHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHHCCCCCCHHHHHHHHHCCCCH
AGMFIDALERAAGKASGGLGRKLA
HHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRILFIVDPLSTFKIYKDSTFAMMREAAARGYAIYTCQQSQLTLSGNVVETVATPLALTG
CEEEEEECCCCCEEEECCCHHHHHHHHHHCCEEEEEEECCCEEECCCCHHHHHCCEEEEC
DEHDWYRSGDPRLLPLTGFDAVLMRKDPPFDMEYVTSTWLLEIAERQGARVFNKPQAIRD
CCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
HSEKLAIAQFREFTAPTIVTRDAKRLREFHAEQGDVIFKPLDGMGGAGIFRVGADGMNLG
HHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCCHH
SVIETLTHNGTRTVMAQQYIPAIRDGDKRILLIGGSPVPHALARVPMAGEVRGNLAAGGT
HHHHHHHCCCCCEEHHHHHCCHHCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCEECCCC
GRAQLLSERDQVIAHALAPVLWQRGLLLVGLDVIGDYLTEVNVTSPTCFQEITQQTGFNV
CHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHHCCCCCCHHHHHHHHHCCCCH
AGMFIDALERAAGKASGGLGRKLA
HHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852