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The map label for this gene is metHa [H]

Identifier: 17545014

GI number: 17545014

Start: 328528

End: 329568

Strand: Reverse

Name: metHa [H]

Synonym: RSc0295

Alternate gene names: 17545014

Gene position: 329568-328528 (Counterclockwise)

Preceding gene: 17545016

Following gene: 17545013

Centisome position: 8.87

GC content: 68.78

Gene sequence:

>1041_bases
ATGACCGCGCCCCTGCCCTACACCCGTGCCGCCAACCTGCCCGCGCTGCTGCGCGAGCGCATCCTGATCCTGGACGGCGC
GATGGGCACCATGATCCAGCGCTACAAGCTGACCGAGGCGCAATACCGCGGCGAGCGCTTCGCCAGCCATCCGGTGGACG
TGAAGGGCAACAACGAACTGCTGCTGCTGACGCGCCCCGAGGTCATCCGCGAGATCCACGAGCAGTACCTGGCCGCGGGC
GCGGACCTGATCGAGACCAACACCTTCGGCGCCACCACCGTCGCGCAGGAAGACTACAAGATGGCGGAGCTGGCCTACGA
GATGAACGTGGTCGCCGCCCGCCTGGCACGCGAGGCCTGCGACAAGTACAGCACGCCGGACAAGCCCCGCTTCGTGGCCG
GTGCCTTCGGCCCGACGCCCAAGACCGCCAGCATCTCGCCCGACGTCAACGACCCGGGCGCGCGCAACATCAGCTTCGAC
CAGCTGCGCGACGCCTACTACGAACAGGGCAAGGCACTGCTCGAAGGCGGCGCCGATGTCTTCCTGGTCGAGACCATCTT
CGACACGCTCAACGCCAAGGCCGCGCTGTTCGCCATCGACCAGCTCTTCGAAGACTCCGGCGAGCGCGTGCCCGTGATGA
TCTCCGGCACCGTCACCGACGCTTCGGGCCGCATCCTGTCGGGCCAGACCGTCGAGGCGTTCTGGAACAGCCTGCGCCAT
GCCAAGCCGATCACCTTCGGCCTGAACTGCGCGCTGGGCGCCGCGTTGATGCGCCCGTACATCGCCGAGCTGGCCAAGAT
CTGCGACACCGCCGTCTCGTGCTACCCCAACGCCGGCCTGCCCAACCCGATGAGCGACACCGGCTTCGATGAAACGCCCG
AGGTCACCTCCAGCCTGGTCGACGAATTCGCGGCCGCCGGCCTGGTGAACCTGGTCGGCGGCTGCTGCGGCACCACGCCC
GAGCACATCCGGGCGATCGCCGAGCGCGTGGCGCAGCGCAAGCCGCGCGCGTGGCCCGGCCAGTACCGCGAAGCCGCCTG
A

Upstream 100 bases:

>100_bases
GCGGCTCCCGGCAATGCCGTCAAGTCCTTGATGCGACTGGTAAAATTCGCCTTTGGCCGCAAATTCGATCGCTTGCCCGG
CCAACCTATCGGAGTCCTCC

Downstream 100 bases:

>100_bases
GACCCAGCCCCACGGCAACCCGTTGAAGCCGGGCGACCACGCCCCCGCACGACCGAACCCCGCACACCATGACCGACCAC
CTCATGCGCCTCTCCGGCCT

Product: 5-methyltetrahydrofolate--homocysteine methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MTAPLPYTRAANLPALLRERILILDGAMGTMIQRYKLTEAQYRGERFASHPVDVKGNNELLLLTRPEVIREIHEQYLAAG
ADLIETNTFGATTVAQEDYKMAELAYEMNVVAARLAREACDKYSTPDKPRFVAGAFGPTPKTASISPDVNDPGARNISFD
QLRDAYYEQGKALLEGGADVFLVETIFDTLNAKAALFAIDQLFEDSGERVPVMISGTVTDASGRILSGQTVEAFWNSLRH
AKPITFGLNCALGAALMRPYIAELAKICDTAVSCYPNAGLPNPMSDTGFDETPEVTSSLVDEFAAAGLVNLVGGCCGTTP
EHIRAIAERVAQRKPRAWPGQYREAA

Sequences:

>Translated_346_residues
MTAPLPYTRAANLPALLRERILILDGAMGTMIQRYKLTEAQYRGERFASHPVDVKGNNELLLLTRPEVIREIHEQYLAAG
ADLIETNTFGATTVAQEDYKMAELAYEMNVVAARLAREACDKYSTPDKPRFVAGAFGPTPKTASISPDVNDPGARNISFD
QLRDAYYEQGKALLEGGADVFLVETIFDTLNAKAALFAIDQLFEDSGERVPVMISGTVTDASGRILSGQTVEAFWNSLRH
AKPITFGLNCALGAALMRPYIAELAKICDTAVSCYPNAGLPNPMSDTGFDETPEVTSSLVDEFAAAGLVNLVGGCCGTTP
EHIRAIAERVAQRKPRAWPGQYREAA
>Mature_345_residues
TAPLPYTRAANLPALLRERILILDGAMGTMIQRYKLTEAQYRGERFASHPVDVKGNNELLLLTRPEVIREIHEQYLAAGA
DLIETNTFGATTVAQEDYKMAELAYEMNVVAARLAREACDKYSTPDKPRFVAGAFGPTPKTASISPDVNDPGARNISFDQ
LRDAYYEQGKALLEGGADVFLVETIFDTLNAKAALFAIDQLFEDSGERVPVMISGTVTDASGRILSGQTVEAFWNSLRHA
KPITFGLNCALGAALMRPYIAELAKICDTAVSCYPNAGLPNPMSDTGFDETPEVTSSLVDEFAAAGLVNLVGGCCGTTPE
HIRAIAERVAQRKPRAWPGQYREAA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=336, Percent_Identity=54.4642857142857, Blast_Score=372, Evalue=1e-103,
Organism=Escherichia coli, GI1790450, Length=334, Percent_Identity=57.7844311377245, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI1786456, Length=328, Percent_Identity=27.7439024390244, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17535405, Length=329, Percent_Identity=52.5835866261398, Blast_Score=353, Evalue=1e-97,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 37555; Mature: 37424

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPLPYTRAANLPALLRERILILDGAMGTMIQRYKLTEAQYRGERFASHPVDVKGNNEL
CCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
LLLTRPEVIREIHEQYLAAGADLIETNTFGATTVAQEDYKMAELAYEMNVVAARLAREAC
EEEECHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKYSTPDKPRFVAGAFGPTPKTASISPDVNDPGARNISFDQLRDAYYEQGKALLEGGADV
HHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHCCCCE
FLVETIFDTLNAKAALFAIDQLFEDSGERVPVMISGTVTDASGRILSGQTVEAFWNSLRH
EEHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCEECCCHHHHHHHHHHH
AKPITFGLNCALGAALMRPYIAELAKICDTAVSCYPNAGLPNPMSDTGFDETPEVTSSLV
CCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
DEFAAAGLVNLVGGCCGTTPEHIRAIAERVAQRKPRAWPGQYREAA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAPLPYTRAANLPALLRERILILDGAMGTMIQRYKLTEAQYRGERFASHPVDVKGNNEL
CCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
LLLTRPEVIREIHEQYLAAGADLIETNTFGATTVAQEDYKMAELAYEMNVVAARLAREAC
EEEECHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKYSTPDKPRFVAGAFGPTPKTASISPDVNDPGARNISFDQLRDAYYEQGKALLEGGADV
HHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHCCCCE
FLVETIFDTLNAKAALFAIDQLFEDSGERVPVMISGTVTDASGRILSGQTVEAFWNSLRH
EEHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCEECCCHHHHHHHHHHH
AKPITFGLNCALGAALMRPYIAELAKICDTAVSCYPNAGLPNPMSDTGFDETPEVTSSLV
CCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
DEFAAAGLVNLVGGCCGTTPEHIRAIAERVAQRKPRAWPGQYREAA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]