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The map label for this gene is metHb [H]

Identifier: 17545013

GI number: 17545013

Start: 325742

End: 328459

Strand: Reverse

Name: metHb [H]

Synonym: RSc0294

Alternate gene names: 17545013

Gene position: 328459-325742 (Counterclockwise)

Preceding gene: 17545014

Following gene: 17545012

Centisome position: 8.84

GC content: 66.11

Gene sequence:

>2718_bases
ATGACCGACCACCTCATGCGCCTCTCCGGCCTCGAACCGTTCAACATCGGCGAGGACACGCTGTTCGTCAACGTCGGCGA
ACGCACCAACGTCACCGGATCCAAGGCGTTCGCGCGCATGATCCTCAACAGCCAGTTCGACGAGGCGCTCGCCGTGGCAC
GCCAGCAGGTCGAGAACGGCGCGCAGGTCATCGACATCAACATGGACGAGGCCATGCTCGACTCCAAGGCGGCGATGGTG
CGCTTCCTGAACCTGATCGCCTCGGAGCCGGACATCGCGCGCGTGCCGATCATGATCGACTCGTCCAAGTGGGAGGTGAT
CGAGGCCGGCCTGAAGTGCGTGCAGGGCAAGGCCATCGTCAACTCGATCTCGCTCAAGGAAGGCGAGGAACAGTTCGCCC
ACCACGCCAAGCTGATCAAGCGCTACGGCGCCGCCGCCGTGGTGATGGCCTTCGACGAGCAGGGCCAGGCCGACACGTTC
GCGCGCAAGACCGAGATCTGCAAGCGCAGCTATGACTTCCTCGTGAACCAGGTCGGCTTTGCGCCGGAAGACATCATCTT
CGATCCGAACATCTTCGCGGTCGCCACCGGCATCGAGGAGCACAACAACTACGCCGTCGACTTCATCGAGGCCACGCGCT
GGATCAAGCAGAAATTGCCGCACGCCAAGGTGAGCGGCGGCGTGTCGAACGTCTCGTTCTCGTTCCGCGGCAACGACGTG
GTGCGCGAGGCCATCCACACCGTGTTCCTGTACCACGCCATCGGTGCGGGCATGGACATGGGCATCGTCAACGCGGGCCA
GTTGGGCGTGTACGAGAACCTCGCCCCCGAACTGCGCGAGCGCGTGGAAGACGTGGTGCTCAACCGCCGCCCGGATGCGA
CCGACCGCCTGCTGGAAATTGCCGACCGCTACAAGGGCGGCGGCGCCAAGCGCGAGGAGAACCTCGCCTGGCGCCAGGAG
CCGGTGGAAAAGCGCCTGGCCCACGCGCTCGTGCACGGCATCACCGACTACGTGGTCGAAGACACCGAGGAAGTTCGCCA
GAAGATCTTTGCCGCCGGCGGCCGCCCGATCCAGGTGATCGAGGGCCCGCTGATGGACGGCATGAACATCGTCGGCGATC
TGTTCGGCGCGGGCAAGATGTTCCTGCCGCAGGTGGTGAAATCCGCCCGCGTGATGAAGCAGGCGGTGGCCCACCTGATC
CCGTTCATCGAGGAAGAGAAGCGGCAGATCGCGGCCGCCGGCGGCGACGTGCGCTCGCGCGGCAAGATCGTCATCGCCAC
CGTGAAGGGCGACGTGCACGACATCGGCAAGAACATCGTCACCGTCGTGCTCCAGTGCAACAACTTCGAAGTCGTGAACA
TGGGCGTGATGGTCCCGTGCAACGAGATCCTGGCCAAGGCGAAGGTCGAGGGCGCGGACATCATCGGCCTGTCGGGCCTG
ATCACACCGTCGCTGGAAGAGATGGCCTACGTGGCCTCCGAGATGCAGCGCGACGAGTACTTCCGCGTGAAGAAGATCCC
GCTGCTGATCGGTGGCGCGACCACGAGCCGCGTGCACACCGCCGTGAAGATCGCGCCCAATTACGAAGGCCCGGTCGTGT
ACGTGCCCGACGCCTCGCGCTCGGTGAGCGTGGCCTCCAGCCTGCTGTCCGACGAGGCCGCCGCGCGCTACATCGAAGAG
CTGCACGCCGACTACGACCGCATCCGCACCCAGCACGCCAGCAAGAAAGCCATGCCGATGGTGTCGCTGGCCGCCGCGCG
CGCCAACAAGACCCGGATCGACTGGTCGAACTACACGCCGCCCAAGCCCAAGTTCGTCGGCCGCCGCGTGTTCCGCAACT
ACGACCTGAACGAGCTCGCGCAGTACATCGACTGGGGCCCGTTCTTCCAGACGTGGGACCTGGCCGGCAAATTCCCCGAC
ATCCTCAACGACGCGATCGTCGGCGAATCGGCCCGCCGCGTGTTCTCCGACGGCAAGAGCATGCTCGCGCGCCTGATCGC
CGGACGCTGGCTGACGGCCAACGGCGTGATCGCGCTGCTGCCGGCCAACACCGTCAACGACGACGACATCGAGATCTACA
CCGACGAGACCCGCTCGGAAGTCGCCCTCACCTGGCGCAACATCCGCCAGCAGAGCGAGCGCCCGATCATCGACGGCGTG
ATGCGCCCGAACCGCTGCCTGGCGGACTTCATCGCCCCCAAGGACACCGGCATCGCCGATTACATCGGCCTCTTCGCGGT
GACGGGCGGCATCGGGATCGACAAGCGCGAAGCCGCCTTCGAAGCCGACCACGACGACTACAGCGCGATCATGCTCAAGG
CCCTGGCCGACCGCTTCGCCGAAGCCTTCGCCGAGTGCCTGCACGCCCGTGTGCGCCGCGACCTGTGGGGCTACGCGCAG
GACGAAACGCTCGACAACGACGCGCTGATCCGCGAGGAATACCGCGGCATCCGCCCGGCGCCCGGCTACCCGGCCTGCCC
GGAGCACACCGTCAAGCGCGACCTGTTCCGCGTGCTCGACGCGCAGGAGATCGGCATGAACCTGACCGAGGCGCTGGCGA
TGACACCGGCCGCGTCGGTCTCGGGCTTCCAGCTGTCGCACCCGGACAGCACGTACTTCACGATCGGCAAGATCGGCCAG
GACCAGGTGGACGACATGGCCGCGCGCAGCGGGGAAGACCGCCGCAATGTGGAGCGCGCCCTGGCACCCAACCTGTAA

Upstream 100 bases:

>100_bases
CGTGGCCCGGCCAGTACCGCGAAGCCGCCTGAGACCCAGCCCCACGGCAACCCGTTGAAGCCGGGCGACCACGCCCCCGC
ACGACCGAACCCCGCACACC

Downstream 100 bases:

>100_bases
ATCTGTGACAAGACGTTTAAGACATGGCGACCGGATCGCCATGTCCGTCATCCCGCCAAGCCGCGCCGGCATTGGGTTGC
GGGGCATTTCAAGCGGTTGC

Product: 5-methyltetrahydrofolate--homocysteine methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 905; Mature: 904

Protein sequence:

>905_residues
MTDHLMRLSGLEPFNIGEDTLFVNVGERTNVTGSKAFARMILNSQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMV
RFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEEQFAHHAKLIKRYGAAAVVMAFDEQGQADTF
ARKTEICKRSYDFLVNQVGFAPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQKLPHAKVSGGVSNVSFSFRGNDV
VREAIHTVFLYHAIGAGMDMGIVNAGQLGVYENLAPELRERVEDVVLNRRPDATDRLLEIADRYKGGGAKREENLAWRQE
PVEKRLAHALVHGITDYVVEDTEEVRQKIFAAGGRPIQVIEGPLMDGMNIVGDLFGAGKMFLPQVVKSARVMKQAVAHLI
PFIEEEKRQIAAAGGDVRSRGKIVIATVKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL
ITPSLEEMAYVASEMQRDEYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASRSVSVASSLLSDEAAARYIEE
LHADYDRIRTQHASKKAMPMVSLAAARANKTRIDWSNYTPPKPKFVGRRVFRNYDLNELAQYIDWGPFFQTWDLAGKFPD
ILNDAIVGESARRVFSDGKSMLARLIAGRWLTANGVIALLPANTVNDDDIEIYTDETRSEVALTWRNIRQQSERPIIDGV
MRPNRCLADFIAPKDTGIADYIGLFAVTGGIGIDKREAAFEADHDDYSAIMLKALADRFAEAFAECLHARVRRDLWGYAQ
DETLDNDALIREEYRGIRPAPGYPACPEHTVKRDLFRVLDAQEIGMNLTEALAMTPAASVSGFQLSHPDSTYFTIGKIGQ
DQVDDMAARSGEDRRNVERALAPNL

Sequences:

>Translated_905_residues
MTDHLMRLSGLEPFNIGEDTLFVNVGERTNVTGSKAFARMILNSQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMV
RFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEEQFAHHAKLIKRYGAAAVVMAFDEQGQADTF
ARKTEICKRSYDFLVNQVGFAPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQKLPHAKVSGGVSNVSFSFRGNDV
VREAIHTVFLYHAIGAGMDMGIVNAGQLGVYENLAPELRERVEDVVLNRRPDATDRLLEIADRYKGGGAKREENLAWRQE
PVEKRLAHALVHGITDYVVEDTEEVRQKIFAAGGRPIQVIEGPLMDGMNIVGDLFGAGKMFLPQVVKSARVMKQAVAHLI
PFIEEEKRQIAAAGGDVRSRGKIVIATVKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL
ITPSLEEMAYVASEMQRDEYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASRSVSVASSLLSDEAAARYIEE
LHADYDRIRTQHASKKAMPMVSLAAARANKTRIDWSNYTPPKPKFVGRRVFRNYDLNELAQYIDWGPFFQTWDLAGKFPD
ILNDAIVGESARRVFSDGKSMLARLIAGRWLTANGVIALLPANTVNDDDIEIYTDETRSEVALTWRNIRQQSERPIIDGV
MRPNRCLADFIAPKDTGIADYIGLFAVTGGIGIDKREAAFEADHDDYSAIMLKALADRFAEAFAECLHARVRRDLWGYAQ
DETLDNDALIREEYRGIRPAPGYPACPEHTVKRDLFRVLDAQEIGMNLTEALAMTPAASVSGFQLSHPDSTYFTIGKIGQ
DQVDDMAARSGEDRRNVERALAPNL
>Mature_904_residues
TDHLMRLSGLEPFNIGEDTLFVNVGERTNVTGSKAFARMILNSQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMVR
FLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEEQFAHHAKLIKRYGAAAVVMAFDEQGQADTFA
RKTEICKRSYDFLVNQVGFAPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQKLPHAKVSGGVSNVSFSFRGNDVV
REAIHTVFLYHAIGAGMDMGIVNAGQLGVYENLAPELRERVEDVVLNRRPDATDRLLEIADRYKGGGAKREENLAWRQEP
VEKRLAHALVHGITDYVVEDTEEVRQKIFAAGGRPIQVIEGPLMDGMNIVGDLFGAGKMFLPQVVKSARVMKQAVAHLIP
FIEEEKRQIAAAGGDVRSRGKIVIATVKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGLI
TPSLEEMAYVASEMQRDEYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASRSVSVASSLLSDEAAARYIEEL
HADYDRIRTQHASKKAMPMVSLAAARANKTRIDWSNYTPPKPKFVGRRVFRNYDLNELAQYIDWGPFFQTWDLAGKFPDI
LNDAIVGESARRVFSDGKSMLARLIAGRWLTANGVIALLPANTVNDDDIEIYTDETRSEVALTWRNIRQQSERPIIDGVM
RPNRCLADFIAPKDTGIADYIGLFAVTGGIGIDKREAAFEADHDDYSAIMLKALADRFAEAFAECLHARVRRDLWGYAQD
ETLDNDALIREEYRGIRPAPGYPACPEHTVKRDLFRVLDAQEIGMNLTEALAMTPAASVSGFQLSHPDSTYFTIGKIGQD
QVDDMAARSGEDRRNVERALAPNL

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=917, Percent_Identity=54.9618320610687, Blast_Score=978, Evalue=0.0,
Organism=Escherichia coli, GI1790450, Length=904, Percent_Identity=62.9424778761062, Blast_Score=1115, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17535405, Length=912, Percent_Identity=54.3859649122807, Blast_Score=949, Evalue=0.0,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 100115; Mature: 99984

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50974 ADOMET_ACTIVATION ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDHLMRLSGLEPFNIGEDTLFVNVGERTNVTGSKAFARMILNSQFDEALAVARQQVENG
CCCCHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCC
AQVIDINMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIV
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHH
NSISLKEGEEQFAHHAKLIKRYGAAAVVMAFDEQGQADTFARKTEICKRSYDFLVNQVGF
HHHHHCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC
APEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQKLPHAKVSGGVSNVSFSFRGNDV
CCCCEEECCCCEEEEECCHHCCCEEEHHHHHHHHHHHHCCCCHHCCCCCCEEEEECCCHH
VREAIHTVFLYHAIGAGMDMGIVNAGQLGVYENLAPELRERVEDVVLNRRPDATDRLLEI
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ADRYKGGGAKREENLAWRQEPVEKRLAHALVHGITDYVVEDTEEVRQKIFAAGGRPIQVI
HHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEE
EGPLMDGMNIVGDLFGAGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRQIAAAGGDVRSR
CCCHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GKIVIATVKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL
CCEEEEEECCCHHHHHHHHEEEEEEECCEEEEEEEEECCHHHHHHHHHCCCCCEEEECCC
ITPSLEEMAYVASEMQRDEYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASR
CCCCHHHHHHHHHHHHHHHHEEEEECCEEEECCCHHHHEEEEEECCCCCCCEEECCCCCC
SVSVASSLLSDEAAARYIEELHADYDRIRTQHASKKAMPMVSLAAARANKTRIDWSNYTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEECCCCCCC
PKPKFVGRRVFRNYDLNELAQYIDWGPFFQTWDLAGKFPDILNDAIVGESARRVFSDGKS
CCHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHH
MLARLIAGRWLTANGVIALLPANTVNDDDIEIYTDETRSEVALTWRNIRQQSERPIIDGV
HHHHHHHCCEEECCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCHHHHC
MRPNRCLADFIAPKDTGIADYIGLFAVTGGIGIDKREAAFEADHDDYSAIMLKALADRFA
CCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
EAFAECLHARVRRDLWGYAQDETLDNDALIREEYRGIRPAPGYPACPEHTVKRDLFRVLD
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
AQEIGMNLTEALAMTPAASVSGFQLSHPDSTYFTIGKIGQDQVDDMAARSGEDRRNVERA
HHHHCCCHHHHHHHCCCCCCCCEEECCCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHH
LAPNL
HCCCC
>Mature Secondary Structure 
TDHLMRLSGLEPFNIGEDTLFVNVGERTNVTGSKAFARMILNSQFDEALAVARQQVENG
CCCHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCC
AQVIDINMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIV
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHH
NSISLKEGEEQFAHHAKLIKRYGAAAVVMAFDEQGQADTFARKTEICKRSYDFLVNQVGF
HHHHHCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC
APEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQKLPHAKVSGGVSNVSFSFRGNDV
CCCCEEECCCCEEEEECCHHCCCEEEHHHHHHHHHHHHCCCCHHCCCCCCEEEEECCCHH
VREAIHTVFLYHAIGAGMDMGIVNAGQLGVYENLAPELRERVEDVVLNRRPDATDRLLEI
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ADRYKGGGAKREENLAWRQEPVEKRLAHALVHGITDYVVEDTEEVRQKIFAAGGRPIQVI
HHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEE
EGPLMDGMNIVGDLFGAGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRQIAAAGGDVRSR
CCCHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GKIVIATVKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL
CCEEEEEECCCHHHHHHHHEEEEEEECCEEEEEEEEECCHHHHHHHHHCCCCCEEEECCC
ITPSLEEMAYVASEMQRDEYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASR
CCCCHHHHHHHHHHHHHHHHEEEEECCEEEECCCHHHHEEEEEECCCCCCCEEECCCCCC
SVSVASSLLSDEAAARYIEELHADYDRIRTQHASKKAMPMVSLAAARANKTRIDWSNYTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEECCCCCCC
PKPKFVGRRVFRNYDLNELAQYIDWGPFFQTWDLAGKFPDILNDAIVGESARRVFSDGKS
CCHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHH
MLARLIAGRWLTANGVIALLPANTVNDDDIEIYTDETRSEVALTWRNIRQQSERPIIDGV
HHHHHHHCCEEECCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCHHHHC
MRPNRCLADFIAPKDTGIADYIGLFAVTGGIGIDKREAAFEADHDDYSAIMLKALADRFA
CCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
EAFAECLHARVRRDLWGYAQDETLDNDALIREEYRGIRPAPGYPACPEHTVKRDLFRVLD
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
AQEIGMNLTEALAMTPAASVSGFQLSHPDSTYFTIGKIGQDQVDDMAARSGEDRRNVERA
HHHHCCCHHHHHHHCCCCCCCCEEECCCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHH
LAPNL
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]