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The map label for this gene is menB [C]

Identifier: 17544986

GI number: 17544986

Start: 300358

End: 301152

Strand: Reverse

Name: menB [C]

Synonym: RSc0267

Alternate gene names: 17544986

Gene position: 301152-300358 (Counterclockwise)

Preceding gene: 17544987

Following gene: 17544985

Centisome position: 8.1

GC content: 71.07

Gene sequence:

>795_bases
ATGAACGCCTTCGAGACCCTCGCCTTCGCACAGCACGGCGCCGTGGCTACGGTCACGCTCAACCGGCCGGACGTGCGCAA
CGCCTTCAACGAGACCGTCATCGCCGAGCTGACCGGGGCGTTCCACGCCCTGGCCACGGAAGACAGCGTGCGCGCCATCG
TGCTGGCCGGCCACGGCCCGGCCTTCTGCGCGGGCGCGGACCTGAACTGGATGCGGAAGATGGCCGGCTACTCCGACGAC
GAAAACCGCGCCGATGCCCTGCGCCTGGCCGAGATGCTGCGCGCCATCCACGCCTGCCCGAAGCCCGTGGTCGCCCGCGT
GCACGGCGACACCTACGCGGGCGGCGTTGGCCTGGTGTCGGCCTGCGACATCGTCGTAGCGGCGGATACCGCACACTTCT
GCCTGTCGGAGGCCAGGCTCGGCCTGATTCCGGCTACTATCAGCCCCTACGTGATCCGCGCGCTGGGCGAACAGGCCTCG
CGCCGCTACTTCATCACCGCCGAGCGCTTCACCGCGGCCGAGGCGCACCGCCTGGGCCTCGTCCATGAACTGGCGGGCGC
CGAGGCGCTGGACGCCACGGTGGACGCGCTCACCGCCGCGCTGGTGGCCAACAGCCCCAACGCGGTGCGGGAAAGCAAGC
GGCTGGTGCGCGAGATCGCCAGCGCGCCGATCGACGACGCGCTGCTGGCCGACACCGCCAACCGCATCGCCGCCATCCGC
GCCTCCACCGAAGGCCGCGAAGGCGTGCAGAGTTTCCTGGGCAAGCGCAAACCGAACTGGCTGCTGCCGGCCTGA

Upstream 100 bases:

>100_bases
CGGAGGTCGACGCCTTCCTGCGCGAGCTTGGCCTGCCCAGCGGCCCCGTCCCCGCCTCCACACCGGCCAGCGCATCGGCC
GATGCCAACCAGGACACGCA

Downstream 100 bases:

>100_bases
CCCCATTCATTATTTGTTCGTTTCAAGGAAGTCCCATGCAACACGCCCCGCTCAACTTCGTTCCCGATGCCGCCAAGGTG
CCGCCCACGCCCGGCCAGAG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MNAFETLAFAQHGAVATVTLNRPDVRNAFNETVIAELTGAFHALATEDSVRAIVLAGHGPAFCAGADLNWMRKMAGYSDD
ENRADALRLAEMLRAIHACPKPVVARVHGDTYAGGVGLVSACDIVVAADTAHFCLSEARLGLIPATISPYVIRALGEQAS
RRYFITAERFTAAEAHRLGLVHELAGAEALDATVDALTAALVANSPNAVRESKRLVREIASAPIDDALLADTANRIAAIR
ASTEGREGVQSFLGKRKPNWLLPA

Sequences:

>Translated_264_residues
MNAFETLAFAQHGAVATVTLNRPDVRNAFNETVIAELTGAFHALATEDSVRAIVLAGHGPAFCAGADLNWMRKMAGYSDD
ENRADALRLAEMLRAIHACPKPVVARVHGDTYAGGVGLVSACDIVVAADTAHFCLSEARLGLIPATISPYVIRALGEQAS
RRYFITAERFTAAEAHRLGLVHELAGAEALDATVDALTAALVANSPNAVRESKRLVREIASAPIDDALLADTANRIAAIR
ASTEGREGVQSFLGKRKPNWLLPA
>Mature_264_residues
MNAFETLAFAQHGAVATVTLNRPDVRNAFNETVIAELTGAFHALATEDSVRAIVLAGHGPAFCAGADLNWMRKMAGYSDD
ENRADALRLAEMLRAIHACPKPVVARVHGDTYAGGVGLVSACDIVVAADTAHFCLSEARLGLIPATISPYVIRALGEQAS
RRYFITAERFTAAEAHRLGLVHELAGAEALDATVDALTAALVANSPNAVRESKRLVREIASAPIDDALLADTANRIAAIR
ASTEGREGVQSFLGKRKPNWLLPA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=28.6852589641434, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI70995211, Length=256, Percent_Identity=32.03125, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI45643119, Length=256, Percent_Identity=28.125, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI260275230, Length=256, Percent_Identity=28.125, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI260274832, Length=256, Percent_Identity=28.125, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI31542718, Length=258, Percent_Identity=25.1937984496124, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI37594469, Length=193, Percent_Identity=29.0155440414508, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI37594471, Length=193, Percent_Identity=29.0155440414508, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI68989263, Length=222, Percent_Identity=27.9279279279279, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI116235487, Length=232, Percent_Identity=26.2931034482759, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4502327, Length=263, Percent_Identity=27.7566539923954, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI221136756, Length=213, Percent_Identity=25.3521126760563, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136753, Length=213, Percent_Identity=25.3521126760563, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221307494, Length=197, Percent_Identity=26.3959390862944, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI4757966, Length=212, Percent_Identity=25.4716981132075, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI51538581, Length=212, Percent_Identity=25.4716981132075, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI25453481, Length=212, Percent_Identity=25.4716981132075, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI51538573, Length=212, Percent_Identity=25.4716981132075, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1788597, Length=261, Percent_Identity=29.8850574712644, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=29.8449612403101, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787660, Length=251, Percent_Identity=29.8804780876494, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=27.5862068965517, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=25.7692307692308, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788682, Length=223, Percent_Identity=28.6995515695067, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=30.3643724696356, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=230, Percent_Identity=30, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=30.3643724696356, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=220, Percent_Identity=30.4545454545455, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=205, Percent_Identity=26.8292682926829, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=171, Percent_Identity=30.4093567251462, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=210, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=219, Percent_Identity=24.2009132420091, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=264, Percent_Identity=25.7575757575758, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653139, Length=269, Percent_Identity=31.9702602230483, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=26.2948207171315, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920382, Length=227, Percent_Identity=32.15859030837, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=29.3877551020408, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=29.3877551020408, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24654903, Length=221, Percent_Identity=23.5294117647059, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27991; Mature: 27991

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAFETLAFAQHGAVATVTLNRPDVRNAFNETVIAELTGAFHALATEDSVRAIVLAGHGP
CCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
AFCAGADLNWMRKMAGYSDDENRADALRLAEMLRAIHACPKPVVARVHGDTYAGGVGLVS
CEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHH
ACDIVVAADTAHFCLSEARLGLIPATISPYVIRALGEQASRRYFITAERFTAAEAHRLGL
HHCEEEECCHHHHHHHHHCCCEECCHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHH
VHELAGAEALDATVDALTAALVANSPNAVRESKRLVREIASAPIDDALLADTANRIAAIR
HHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEE
ASTEGREGVQSFLGKRKPNWLLPA
CCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNAFETLAFAQHGAVATVTLNRPDVRNAFNETVIAELTGAFHALATEDSVRAIVLAGHGP
CCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
AFCAGADLNWMRKMAGYSDDENRADALRLAEMLRAIHACPKPVVARVHGDTYAGGVGLVS
CEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHH
ACDIVVAADTAHFCLSEARLGLIPATISPYVIRALGEQASRRYFITAERFTAAEAHRLGL
HHCEEEECCHHHHHHHHHCCCEECCHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHH
VHELAGAEALDATVDALTAALVANSPNAVRESKRLVREIASAPIDDALLADTANRIAAIR
HHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEE
ASTEGREGVQSFLGKRKPNWLLPA
CCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA