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The map label for this gene is bioB

Identifier: 17544985

GI number: 17544985

Start: 299240

End: 300322

Strand: Reverse

Name: bioB

Synonym: RSc0266

Alternate gene names: 17544985

Gene position: 300322-299240 (Counterclockwise)

Preceding gene: 17544986

Following gene: 17544984

Centisome position: 8.08

GC content: 67.5

Gene sequence:

>1083_bases
ATGCAACACGCCCCGCTCAACTTCGTTCCCGATGCCGCCAAGGTGCCGCCCACGCCCGGCCAGAGCCCGAACGCGCGCTG
GAGCCGCGAGGCCATCGAAGCGCTGTTCGCGCTGCCGTTCAACGACCTGCTGTTCCAGGCGCAGCAGGTGCACCGCGCCC
ACTTCGACGCCAACGCGGTGCAGCTCTCCACGCTGCTGTCGATCAAGACCGGCGGCTGCCCCGAGGATTGCTCCTACTGC
CCGCAATCGGCCCGCTACGACACCGGCGTCGAGGCCGAGAAGCTGATGCCGATCGACACCGTGCTCGAAGCCGCCGCCCG
CGCCAAGCAGAACGGCGCCAGCCGCTTCTGCATGGGCGCGGCCTGGCGCAACCCCAAGCCGCACCAGCTCGATGCCGTGG
CCGACATGGTGCGCGGGGTCAAGGCGATGGGCCTGGAGACCTGCGTCACGCTCGGCATGCTCAAGCAGGAGCAGGCCCAG
CAGCTCAAGGATGCCGGCCTCGACTACTACAACCACAACCTCGACACCGCGCCCGAGTTCTACGGCGAGATCATCACCAC
GCGCACCTACCAGGACCGCCTCGACACCCTCGAGCACGTGCGCGACGCCGGCATCAACGTGTGCTGCGGTGGCATCGTCG
GCCTGGGCGAATCGCGCCACGAGCGCGCGGGGCTGGTCGCCGAGCTGGCCAACATGGAGCCGTATCCGGATTCGGTGCCG
ATCAACAACCTGGTCAAGGTCGAAGGCACGCCGCTGGCGGGCAACGAAGCGCTCGACCCATTCGAGTTCGTGCGCACCAT
CGCCGTGGCGCGGATCACCATGCCCAAGGCCATGGTGCGGCTGTCCGCCGGCCGCGAAGCGATGGACGACGCGCTGCAGG
CGCTGTGCTTCATGGCGGGGGCCAACTCGATCTTCTACGGCGAGAAGCTGCTGACGACCGACAACCCGGAAGCCGATGCC
GATCGCAAGCTGCTGGCGCGGCTGGGGATGCGGGTGGAGGTGCAGGATCATCTGCATCAGGCGGAGGGGGCGCAGCATTC
GCATTCGTCGCATTGTCATATCGACATTACGCCGGCTGATTGA

Upstream 100 bases:

>100_bases
AAGGCCGCGAAGGCGTGCAGAGTTTCCTGGGCAAGCGCAAACCGAACTGGCTGCTGCCGGCCTGACCCCATTCATTATTT
GTTCGTTTCAAGGAAGTCCC

Downstream 100 bases:

>100_bases
TTGGTTTTTTGTGGGGATGGGCTGGTTTTTTTTGTTGGTGGTTCATCCTCGGTTTCGTCCCCTGCCGGGGCCGACTCACT
TTCTTTGTCTTGCCAAAGAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MQHAPLNFVPDAAKVPPTPGQSPNARWSREAIEALFALPFNDLLFQAQQVHRAHFDANAVQLSTLLSIKTGGCPEDCSYC
PQSARYDTGVEAEKLMPIDTVLEAAARAKQNGASRFCMGAAWRNPKPHQLDAVADMVRGVKAMGLETCVTLGMLKQEQAQ
QLKDAGLDYYNHNLDTAPEFYGEIITTRTYQDRLDTLEHVRDAGINVCCGGIVGLGESRHERAGLVAELANMEPYPDSVP
INNLVKVEGTPLAGNEALDPFEFVRTIAVARITMPKAMVRLSAGREAMDDALQALCFMAGANSIFYGEKLLTTDNPEADA
DRKLLARLGMRVEVQDHLHQAEGAQHSHSSHCHIDITPAD

Sequences:

>Translated_360_residues
MQHAPLNFVPDAAKVPPTPGQSPNARWSREAIEALFALPFNDLLFQAQQVHRAHFDANAVQLSTLLSIKTGGCPEDCSYC
PQSARYDTGVEAEKLMPIDTVLEAAARAKQNGASRFCMGAAWRNPKPHQLDAVADMVRGVKAMGLETCVTLGMLKQEQAQ
QLKDAGLDYYNHNLDTAPEFYGEIITTRTYQDRLDTLEHVRDAGINVCCGGIVGLGESRHERAGLVAELANMEPYPDSVP
INNLVKVEGTPLAGNEALDPFEFVRTIAVARITMPKAMVRLSAGREAMDDALQALCFMAGANSIFYGEKLLTTDNPEADA
DRKLLARLGMRVEVQDHLHQAEGAQHSHSSHCHIDITPAD
>Mature_360_residues
MQHAPLNFVPDAAKVPPTPGQSPNARWSREAIEALFALPFNDLLFQAQQVHRAHFDANAVQLSTLLSIKTGGCPEDCSYC
PQSARYDTGVEAEKLMPIDTVLEAAARAKQNGASRFCMGAAWRNPKPHQLDAVADMVRGVKAMGLETCVTLGMLKQEQAQ
QLKDAGLDYYNHNLDTAPEFYGEIITTRTYQDRLDTLEHVRDAGINVCCGGIVGLGESRHERAGLVAELANMEPYPDSVP
INNLVKVEGTPLAGNEALDPFEFVRTIAVARITMPKAMVRLSAGREAMDDALQALCFMAGANSIFYGEKLLTTDNPEADA
DRKLLARLGMRVEVQDHLHQAEGAQHSHSSHCHIDITPAD

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=305, Percent_Identity=66.5573770491803, Blast_Score=435, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6321725, Length=318, Percent_Identity=50.314465408805, Blast_Score=339, Evalue=4e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_RALSO (Q8Y2R9)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518387.1
- HSSP:   P12996
- ProteinModelPortal:   Q8Y2R9
- SMR:   Q8Y2R9
- GeneID:   1219069
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0266
- NMPDR:   fig|267608.1.peg.266
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2302364
- BioCyc:   RSOL267608:RSC0266-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 39300; Mature: 39300

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHAPLNFVPDAAKVPPTPGQSPNARWSREAIEALFALPFNDLLFQAQQVHRAHFDANAV
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHH
QLSTLLSIKTGGCPEDCSYCPQSARYDTGVEAEKLMPIDTVLEAAARAKQNGASRFCMGA
HHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCHHHHHH
AWRNPKPHQLDAVADMVRGVKAMGLETCVTLGMLKQEQAQQLKDAGLDYYNHNLDTAPEF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHH
YGEIITTRTYQDRLDTLEHVRDAGINVCCGGIVGLGESRHERAGLVAELANMEPYPDSVP
HHHHHHCCHHHHHHHHHHHHHHCCCCEEECCHHCCCCCHHHHHHHHHHHHCCCCCCCCCC
INNLVKVEGTPLAGNEALDPFEFVRTIAVARITMPKAMVRLSAGREAMDDALQALCFMAG
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
ANSIFYGEKLLTTDNPEADADRKLLARLGMRVEVQDHLHQAEGAQHSHSSHCHIDITPAD
CCCEEECCEEECCCCCCCCHHHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MQHAPLNFVPDAAKVPPTPGQSPNARWSREAIEALFALPFNDLLFQAQQVHRAHFDANAV
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHH
QLSTLLSIKTGGCPEDCSYCPQSARYDTGVEAEKLMPIDTVLEAAARAKQNGASRFCMGA
HHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCHHHHHH
AWRNPKPHQLDAVADMVRGVKAMGLETCVTLGMLKQEQAQQLKDAGLDYYNHNLDTAPEF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHH
YGEIITTRTYQDRLDTLEHVRDAGINVCCGGIVGLGESRHERAGLVAELANMEPYPDSVP
HHHHHHCCHHHHHHHHHHHHHHCCCCEEECCHHCCCCCHHHHHHHHHHHHCCCCCCCCCC
INNLVKVEGTPLAGNEALDPFEFVRTIAVARITMPKAMVRLSAGREAMDDALQALCFMAG
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
ANSIFYGEKLLTTDNPEADADRKLLARLGMRVEVQDHLHQAEGAQHSHSSHCHIDITPAD
CCCEEECCEEECCCCCCCCHHHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852