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The map label for this gene is rtcA

Identifier: 17544945

GI number: 17544945

Start: 248347

End: 249390

Strand: Direct

Name: rtcA

Synonym: RSc0226

Alternate gene names: 17544945

Gene position: 248347-249390 (Clockwise)

Preceding gene: 17544944

Following gene: 17544946

Centisome position: 6.68

GC content: 72.32

Gene sequence:

>1044_bases
ATGAACAACCGACTACAACCCGCGCCCTGCATCGAGCTCGATGGTGCCCAAGGCGAGGGCGGCGGACAGATCCTGCGCAC
GGCATTGACGCTGTCGATGCTGACCGGCACCCCCTTTCGCATCGAGCGGATCCGCGCGGGCCGGTCCAAGCCCGGTCTGA
TGCGCCAGCACCTGACGGCGGTGCAGGCTGCGGCGGAGGTCAGTGGCGCCACAGTAGAGGGCGCCGAGGCCGGGTCGCAG
GCGCTGGCCTTTGCGCCGGGGCCGATCCACGGCGGTGACTACCGCTTCGCGATCGGCACAGCCGGCAGCTGCACGCTGGT
GCTGCAGACCGTCCTGCCGGCGCTGTGGTTTGCCGATGCGCCGAGCACCGTGGCGGTGAGCGGCGGCACGCACAACCGCG
CGGCGCCGCCGGTCGATTTCCTGATCCGCGCATGGCAGCCGCTGCTGGCCCGCATGGGCGTGACGCAGACGCTCGCGCTC
AAGCGTCACGGCTTCTATCCGGCCGGCGGCGGCGAAGTGCTGGCCACGGTCACGCCGTGCGCCGGCCGGCTCGGCGCGCT
GCACCTGACCGAGCGCGGCGCGCTGCGCGAGCTGAGCGGCCAGGGGATCGTCGCCAATGTGCGGCCGGGCGTGGCCCGCC
GGGAACTGGAGGCGCTGGCCGCGCGCGTGCCCGGCGTGGTCGGCTCGGTGCGCGAGCTGTCGCCCGCCGAGGGGCCGGGC
AACGCGCTGGTGCTCGACGCCGTGCATGAGCACGTGACTGAAGTCTTCACCGGCTTCGGCGAGCGCGGCGTGCCGGCCGA
GCAGGTGGCGCACGGGGTCGCAAGCGCTGCGCTGCGCTATCTGCACAGCACCGCCGCGGTCGACGAGTACCTCGCCGATC
AGCTGGTGCTGCCGATGGCGCTGGCCGGCGCGGGCTGCTTCACCGCCGTGACCGCATCGCCGCACCTGACGACCAACATC
GCGGTGATCGAGAAGTTCCTGCCGGTCCACATCACTGTGCAGGCGGAGCGTGATGCCTCGGTGGTGCGGGTGCAGGTCAA
CTGA

Upstream 100 bases:

>100_bases
GGCGCTGGATGCGTTCCTCTATGCAACCGTGATGGCGGCGGACACCCATCCTTCCTGATGCGCCCGGCATGGGCGCGCAA
CGAACAACAATAAACAAGTC

Downstream 100 bases:

>100_bases
CGACGTGCGGCGATGGCGTCGCTCGAGGCGGCGCTGTTTGATCCGGCATGCACTTCGCATGCCGGGCCGGCCGGGATGTC
CCGAAAGAGCGTGGGCCAGC

Product: RNA 3'-terminal-phosphate cyclase

Products: NA

Alternate protein names: RNA cyclase; RNA-3'-phosphate cyclase

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MNNRLQPAPCIELDGAQGEGGGQILRTALTLSMLTGTPFRIERIRAGRSKPGLMRQHLTAVQAAAEVSGATVEGAEAGSQ
ALAFAPGPIHGGDYRFAIGTAGSCTLVLQTVLPALWFADAPSTVAVSGGTHNRAAPPVDFLIRAWQPLLARMGVTQTLAL
KRHGFYPAGGGEVLATVTPCAGRLGALHLTERGALRELSGQGIVANVRPGVARRELEALAARVPGVVGSVRELSPAEGPG
NALVLDAVHEHVTEVFTGFGERGVPAEQVAHGVASAALRYLHSTAAVDEYLADQLVLPMALAGAGCFTAVTASPHLTTNI
AVIEKFLPVHITVQAERDASVVRVQVN

Sequences:

>Translated_347_residues
MNNRLQPAPCIELDGAQGEGGGQILRTALTLSMLTGTPFRIERIRAGRSKPGLMRQHLTAVQAAAEVSGATVEGAEAGSQ
ALAFAPGPIHGGDYRFAIGTAGSCTLVLQTVLPALWFADAPSTVAVSGGTHNRAAPPVDFLIRAWQPLLARMGVTQTLAL
KRHGFYPAGGGEVLATVTPCAGRLGALHLTERGALRELSGQGIVANVRPGVARRELEALAARVPGVVGSVRELSPAEGPG
NALVLDAVHEHVTEVFTGFGERGVPAEQVAHGVASAALRYLHSTAAVDEYLADQLVLPMALAGAGCFTAVTASPHLTTNI
AVIEKFLPVHITVQAERDASVVRVQVN
>Mature_347_residues
MNNRLQPAPCIELDGAQGEGGGQILRTALTLSMLTGTPFRIERIRAGRSKPGLMRQHLTAVQAAAEVSGATVEGAEAGSQ
ALAFAPGPIHGGDYRFAIGTAGSCTLVLQTVLPALWFADAPSTVAVSGGTHNRAAPPVDFLIRAWQPLLARMGVTQTLAL
KRHGFYPAGGGEVLATVTPCAGRLGALHLTERGALRELSGQGIVANVRPGVARRELEALAARVPGVVGSVRELSPAEGPG
NALVLDAVHEHVTEVFTGFGERGVPAEQVAHGVASAALRYLHSTAAVDEYLADQLVLPMALAGAGCFTAVTASPHLTTNI
AVIEKFLPVHITVQAERDASVVRVQVN

Specific function: Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps:(A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A;

COG id: COG0430

COG function: function code A; RNA 3'-terminal phosphate cyclase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4506589, Length=346, Percent_Identity=34.971098265896, Blast_Score=198, Evalue=6e-51,
Organism=Homo sapiens, GI195927044, Length=359, Percent_Identity=33.7047353760446, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI157426877, Length=202, Percent_Identity=28.7128712871287, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI48994937, Length=337, Percent_Identity=59.0504451038576, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32564710, Length=214, Percent_Identity=26.1682242990654, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324564, Length=280, Percent_Identity=23.5714285714286, Blast_Score=62, Evalue=9e-11,
Organism=Drosophila melanogaster, GI18543269, Length=337, Percent_Identity=32.0474777448071, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI18860065, Length=203, Percent_Identity=28.0788177339901, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RTCA_RALSO (Q8Y2V6)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518347.1
- ProteinModelPortal:   Q8Y2V6
- SMR:   Q8Y2V6
- GeneID:   1219029
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0226
- NMPDR:   fig|267608.1.peg.226
- HOGENOM:   HBG683539
- OMA:   EIDGSYG
- ProtClustDB:   PRK04204
- BioCyc:   RSOL267608:RSC0226-MONOMER
- BRENDA:   6.5.1.4
- GO:   GO:0005737
- GO:   GO:0005654
- HAMAP:   MF_00200
- InterPro:   IPR013791
- InterPro:   IPR000228
- InterPro:   IPR020719
- InterPro:   IPR013796
- InterPro:   IPR020723
- InterPro:   IPR017770
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.20
- PANTHER:   PTHR11096
- TIGRFAMs:   TIGR03399

Pfam domain/function: PF01137 RTC; PF05189 RTC_insert; SSF52913 RNA3'-term_phos_cycl_insert; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =6.5.1.4

Molecular weight: Translated: 35971; Mature: 35971

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS01287 RTC

Important sites: ACT_SITE 315-315 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNRLQPAPCIELDGAQGEGGGQILRTALTLSMLTGTPFRIERIRAGRSKPGLMRQHLTA
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCHHHHHHHH
VQAAAEVSGATVEGAEAGSQALAFAPGPIHGGDYRFAIGTAGSCTLVLQTVLPALWFADA
HHHHHHHCCCEECCCCCCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCC
PSTVAVSGGTHNRAAPPVDFLIRAWQPLLARMGVTQTLALKRHGFYPAGGGEVLATVTPC
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEEECCC
AGRLGALHLTERGALRELSGQGIVANVRPGVARRELEALAARVPGVVGSVRELSPAEGPG
CCCCCEEEECCCCHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC
NALVLDAVHEHVTEVFTGFGERGVPAEQVAHGVASAALRYLHSTAAVDEYLADQLVLPMA
CEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGAGCFTAVTASPHLTTNIAVIEKFLPVHITVQAERDASVVRVQVN
HHCCCHHEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCEEEEEEEC
>Mature Secondary Structure
MNNRLQPAPCIELDGAQGEGGGQILRTALTLSMLTGTPFRIERIRAGRSKPGLMRQHLTA
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCHHHHHHHH
VQAAAEVSGATVEGAEAGSQALAFAPGPIHGGDYRFAIGTAGSCTLVLQTVLPALWFADA
HHHHHHHCCCEECCCCCCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCC
PSTVAVSGGTHNRAAPPVDFLIRAWQPLLARMGVTQTLALKRHGFYPAGGGEVLATVTPC
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEEECCC
AGRLGALHLTERGALRELSGQGIVANVRPGVARRELEALAARVPGVVGSVRELSPAEGPG
CCCCCEEEECCCCHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC
NALVLDAVHEHVTEVFTGFGERGVPAEQVAHGVASAALRYLHSTAAVDEYLADQLVLPMA
CEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGAGCFTAVTASPHLTTNIAVIEKFLPVHITVQAERDASVVRVQVN
HHCCCHHEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852