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The map label for this gene is ttcA [H]

Identifier: 17544894

GI number: 17544894

Start: 197176

End: 198114

Strand: Direct

Name: ttcA [H]

Synonym: RSc0175

Alternate gene names: 17544894

Gene position: 197176-198114 (Clockwise)

Preceding gene: 17544885

Following gene: 17544896

Centisome position: 5.31

GC content: 65.18

Gene sequence:

>939_bases
ATGACGTTCTCCAACAATTTCCACCGCCTCGAAACCCGCCTGCAGTCGCAGGCCGGCCGCGCCATCGGCGACTTCAAGAT
GATCGAGGACGGCGACACCGTGCTGGTCTGCCTGTCCGGCGGCAAGGATTCGTACACCATGCTGTCGGTGCTGATGGCCC
TGCAGAAGCGCGCGCCGATCGACTTCAAGCTGATCGCGATGAACCTCGATCAGAAGCAGCCGGGGTTTCCGGAGGACGTG
CTGCCCGGGTATCTGAAGAAGGTGGGCGTGGAGTACGTGATCGTCGAGGCCGACACGTATTCGATCGTCAAGGAGAAGGT
GCCCGAGGGCAAGACCACGTGCTCGCTGTGCTCGCGCCTGCGCCGCGGCGTGATCTATCGCACGGCCAAGGAGCTGGGCG
CCAACAAGATCGCGTTGGGCCACCACCGCGACGACATCGTCAACACGTTCTTCCTGAACATGTTCTTCGGCGGCAAGATG
AAGGCGATGCCGCCCAAGCTCGCCACCGACAACGGCGACCACATCGTGATCCGTCCGCTGGCCTACTGCGCGGAGAAGGA
GATCGCCGCCTACGCCCGCGCGATGGAGTTCCCCATCATCCCGTGCAACCTGTGCGGCTCGCAGGAGAACCTGCAGCGCA
AGAAGGTCAAGGAGATGCTGCTCGAGTGGGAGCGCCAGGCGCCGGGCCGCATCGACAACATCTTCTCGGCGCTGCAGAAC
GTGGTGCCGTCGCACCTGGCCGATACCGACCTGTTCGACTTCAACGGCCTGACCACCGGCCTGGCCAAGATCGGCGAGGA
GGCGCTGTTCGGCCAGACCGCGTACGACCAGGCGCCGCTGGTGTTCGCCGGCTCGCACGACGATCGCATCGAGTTCGTGC
GCTTCGAGCGCAATCCGGCGGGCAAGGCGCCGGAGGGCGCCGAGCAGCCCGCGGCCTGA

Upstream 100 bases:

>100_bases
GCGCGCGCCCACGGCTGGCCGTGGCACGCTAAAATGCTGATTTTCCAGACAATTTGACGCCCGATCGGCCATTCTTCAGC
GCCGGCGGCGTGCTTGCGCC

Downstream 100 bases:

>100_bases
CGCACCGCATCGGCAACACGGACAGCGTGCCGGGTCGTGGGGCCGGGCACGCTGCCCTGTTTTGGAAGGCTGGCGCGCTG
CCGCTCAGCGGTTGCGCGGC

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTFSNNFHRLETRLQSQAGRAIGDFKMIEDGDTVLVCLSGGKDSYTMLSVLMALQKRAPIDFKLIAMNLDQKQPGFPEDV
LPGYLKKVGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRLRRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKM
KAMPPKLATDNGDHIVIRPLAYCAEKEIAAYARAMEFPIIPCNLCGSQENLQRKKVKEMLLEWERQAPGRIDNIFSALQN
VVPSHLADTDLFDFNGLTTGLAKIGEEALFGQTAYDQAPLVFAGSHDDRIEFVRFERNPAGKAPEGAEQPAA

Sequences:

>Translated_312_residues
MTFSNNFHRLETRLQSQAGRAIGDFKMIEDGDTVLVCLSGGKDSYTMLSVLMALQKRAPIDFKLIAMNLDQKQPGFPEDV
LPGYLKKVGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRLRRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKM
KAMPPKLATDNGDHIVIRPLAYCAEKEIAAYARAMEFPIIPCNLCGSQENLQRKKVKEMLLEWERQAPGRIDNIFSALQN
VVPSHLADTDLFDFNGLTTGLAKIGEEALFGQTAYDQAPLVFAGSHDDRIEFVRFERNPAGKAPEGAEQPAA
>Mature_311_residues
TFSNNFHRLETRLQSQAGRAIGDFKMIEDGDTVLVCLSGGKDSYTMLSVLMALQKRAPIDFKLIAMNLDQKQPGFPEDVL
PGYLKKVGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRLRRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKMK
AMPPKLATDNGDHIVIRPLAYCAEKEIAAYARAMEFPIIPCNLCGSQENLQRKKVKEMLLEWERQAPGRIDNIFSALQNV
VPSHLADTDLFDFNGLTTGLAKIGEEALFGQTAYDQAPLVFAGSHDDRIEFVRFERNPAGKAPEGAEQPAA

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=255, Percent_Identity=69.8039215686274, Blast_Score=394, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI193211090, Length=237, Percent_Identity=32.4894514767932, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17540090, Length=212, Percent_Identity=27.8301886792453, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24651972, Length=238, Percent_Identity=26.890756302521, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 34650; Mature: 34519

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSNNFHRLETRLQSQAGRAIGDFKMIEDGDTVLVCLSGGKDSYTMLSVLMALQKRAPI
CCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHCCCC
DFKLIAMNLDQKQPGFPEDVLPGYLKKVGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRL
CEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHHH
RRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKMKAMPPKLATDNGDHIVIRPL
HHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHH
AYCAEKEIAAYARAMEFPIIPCNLCGSQENLQRKKVKEMLLEWERQAPGRIDNIFSALQN
HHHHHHHHHHHHHHHCCCCEEHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VVPSHLADTDLFDFNGLTTGLAKIGEEALFGQTAYDQAPLVFAGSHDDRIEFVRFERNPA
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEEEECCCC
GKAPEGAEQPAA
CCCCCCCCCCCC
>Mature Secondary Structure 
TFSNNFHRLETRLQSQAGRAIGDFKMIEDGDTVLVCLSGGKDSYTMLSVLMALQKRAPI
CCCCHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHCCCC
DFKLIAMNLDQKQPGFPEDVLPGYLKKVGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRL
CEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHHH
RRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKMKAMPPKLATDNGDHIVIRPL
HHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHH
AYCAEKEIAAYARAMEFPIIPCNLCGSQENLQRKKVKEMLLEWERQAPGRIDNIFSALQN
HHHHHHHHHHHHHHHCCCCEEHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VVPSHLADTDLFDFNGLTTGLAKIGEEALFGQTAYDQAPLVFAGSHDDRIEFVRFERNPA
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEEEECCCC
GKAPEGAEQPAA
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA