Click here to switch to the map view.

The map label for this gene is ynfL [H]

Identifier: 17544879

GI number: 17544879

Start: 180020

End: 180946

Strand: Direct

Name: ynfL [H]

Synonym: RSc0160

Alternate gene names: 17544879

Gene position: 180020-180946 (Clockwise)

Preceding gene: 17544876

Following gene: 17544881

Centisome position: 4.84

GC content: 70.87

Gene sequence:

>927_bases
ATGTCCCATTCGCCGCTGCGCTATCTGCATAGTTTCCTGATCGTCGCCAACGAGGGCAGCTTCGTGCGCGCGGCGGATCG
GCTCGCCGTGTCGCAGCCGGCGCTGTCGTACCAGATGCGCCAGCTGGAGCAATGGCTGGGCGTGCCGCTGTTCGAGCGCG
CCGGGCGCAAGCTGGTGCTCACGCGCGCCGGCGCTTCGGTGCGGGCGTGGTGCCGCGACGCGTTCGCCGGGCTGGACGAG
CTGCGCGCCGCATTGCACAGCGGCGAGATGGAGCGCGTATCGCTCAAGCTGGCGAGCGGTTCCAGCTTCGGCCGCCACGT
GCTGATGCCGGCGTTGCTGGACCCGGCCCCCGAGTCCGACGCGCCGCTGCTGGACGACGTGCGGCTGGAGCTGGCCTTCG
GCAGCGACGACGAGGTCTTCGCCCATGTCGAATCGGGCCGCGCCGACCTGGGCTTCGTCTACACGCCGCGCACCTCGCGG
CTGTTCGAGCACCACGCTGTCTATACCGAAGAATTCGTGCTGGCCTGCGGCGCCCGCGCGCTGCGCGAACGCGAGGCGCC
GCGCACGCTGGCCGACTGCGCGGCGCTGCCCTTCGTCACCTACGACGAATCCGATGCGGTGTACGGCCTGTGGTTTCGGG
CGCTGTTCCGGCGCATGCCGGAGACCACCGTCAGCGCGCACCACGTCTCGGAACTGGAGGAGGTCGCCACACTGGTGGCG
GCCGGCATGGGCTGGTCGGTGCTGCCGCTGCATGCGATTCGCGACCCGGTGGCGAGCGCCCGGCTGCAGGTGGTGCGGCC
GGTATCGACGCGGCGCTGCCTCAACACCGTGTTCGCCGTGCGGCGCACCTCGAGCTTTCCCAGCGAGGCGCAGGATCGCT
TGCTGGTGCGATTGGCGCAGCTGGAGGCAGCCGCGGCGACAGCCTAG

Upstream 100 bases:

>100_bases
TTGCGACGGATTGTGCGATCTGCCAAACTCAAAATCAAATCGAATGTTTTTCGTTTTTCGATCCAAAAATTTGATGATTT
GATGCGCTGTTCCCGTCGCC

Downstream 100 bases:

>100_bases
CCCCCCACCTCGAACCGGATACCCTGCGCCAGCGGCAGCGCCGTGCCGTAGTTGACGGTATTGGTGGCGCGGCGCATGTA
GGCCTTCCAGGCATCCGAGC

Product: transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSHSPLRYLHSFLIVANEGSFVRAADRLAVSQPALSYQMRQLEQWLGVPLFERAGRKLVLTRAGASVRAWCRDAFAGLDE
LRAALHSGEMERVSLKLASGSSFGRHVLMPALLDPAPESDAPLLDDVRLELAFGSDDEVFAHVESGRADLGFVYTPRTSR
LFEHHAVYTEEFVLACGARALREREAPRTLADCAALPFVTYDESDAVYGLWFRALFRRMPETTVSAHHVSELEEVATLVA
AGMGWSVLPLHAIRDPVASARLQVVRPVSTRRCLNTVFAVRRTSSFPSEAQDRLLVRLAQLEAAAATA

Sequences:

>Translated_308_residues
MSHSPLRYLHSFLIVANEGSFVRAADRLAVSQPALSYQMRQLEQWLGVPLFERAGRKLVLTRAGASVRAWCRDAFAGLDE
LRAALHSGEMERVSLKLASGSSFGRHVLMPALLDPAPESDAPLLDDVRLELAFGSDDEVFAHVESGRADLGFVYTPRTSR
LFEHHAVYTEEFVLACGARALREREAPRTLADCAALPFVTYDESDAVYGLWFRALFRRMPETTVSAHHVSELEEVATLVA
AGMGWSVLPLHAIRDPVASARLQVVRPVSTRRCLNTVFAVRRTSSFPSEAQDRLLVRLAQLEAAAATA
>Mature_307_residues
SHSPLRYLHSFLIVANEGSFVRAADRLAVSQPALSYQMRQLEQWLGVPLFERAGRKLVLTRAGASVRAWCRDAFAGLDEL
RAALHSGEMERVSLKLASGSSFGRHVLMPALLDPAPESDAPLLDDVRLELAFGSDDEVFAHVESGRADLGFVYTPRTSRL
FEHHAVYTEEFVLACGARALREREAPRTLADCAALPFVTYDESDAVYGLWFRALFRRMPETTVSAHHVSELEEVATLVAA
GMGWSVLPLHAIRDPVASARLQVVRPVSTRRCLNTVFAVRRTSSFPSEAQDRLLVRLAQLEAAAATA

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=34, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI157672245, Length=169, Percent_Identity=35.5029585798817, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1788748, Length=279, Percent_Identity=27.5985663082437, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=26.962457337884, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788887, Length=254, Percent_Identity=28.740157480315, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790208, Length=260, Percent_Identity=29.2307692307692, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082132, Length=256, Percent_Identity=26.953125, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790399, Length=283, Percent_Identity=27.5618374558304, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790783, Length=266, Percent_Identity=25.9398496240602, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33998; Mature: 33867

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50931 HTH_LYSR ; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSPLRYLHSFLIVANEGSFVRAADRLAVSQPALSYQMRQLEQWLGVPLFERAGRKLVL
CCCCHHHHHHHHEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHCCCEEEE
TRAGASVRAWCRDAFAGLDELRAALHSGEMERVSLKLASGSSFGRHVLMPALLDPAPESD
EECCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCCC
APLLDDVRLELAFGSDDEVFAHVESGRADLGFVYTPRTSRLFEHHAVYTEEFVLACGARA
CCCHHCEEEEEEECCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LREREAPRTLADCAALPFVTYDESDAVYGLWFRALFRRMPETTVSAHHVSELEEVATLVA
HHHHCCCHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AGMGWSVLPLHAIRDPVASARLQVVRPVSTRRCLNTVFAVRRTSSFPSEAQDRLLVRLAQ
HCCCCHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LEAAAATA
HHHHHCCC
>Mature Secondary Structure 
SHSPLRYLHSFLIVANEGSFVRAADRLAVSQPALSYQMRQLEQWLGVPLFERAGRKLVL
CCCHHHHHHHHEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHCCCEEEE
TRAGASVRAWCRDAFAGLDELRAALHSGEMERVSLKLASGSSFGRHVLMPALLDPAPESD
EECCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCCC
APLLDDVRLELAFGSDDEVFAHVESGRADLGFVYTPRTSRLFEHHAVYTEEFVLACGARA
CCCHHCEEEEEEECCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LREREAPRTLADCAALPFVTYDESDAVYGLWFRALFRRMPETTVSAHHVSELEEVATLVA
HHHHCCCHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AGMGWSVLPLHAIRDPVASARLQVVRPVSTRRCLNTVFAVRRTSSFPSEAQDRLLVRLAQ
HCCCCHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LEAAAATA
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]