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The map label for this gene is phoA [H]

Identifier: 17544817

GI number: 17544817

Start: 110524

End: 111927

Strand: Direct

Name: phoA [H]

Synonym: RSc0098

Alternate gene names: 17544817

Gene position: 110524-111927 (Clockwise)

Preceding gene: 17544816

Following gene: 17544818

Centisome position: 2.97

GC content: 69.52

Gene sequence:

>1404_bases
ATGACCAAGCAATCGAACCGCATCCTCCGCGCCGGCGCGCTCGCGCTGGCGGCGGCCGCAGCGTTGCCCGTGCTCCCGGC
CCGCGCGGCCGGCGAGGCCAGGAACGTGATCTTCTTCCTGGGCGACGGCATGGGCCCGACCACCGTCACCGCCGCGCGCA
TCTACAAGGCGGGCGAGGCCGGCCAGCTGACCATGGAGTCGCTCAAGCGCACCGCGCGCATCAAGACCTACTCCAACGAC
GCGCAGACCACCGACAGCGCCCCATCGATGTCGGCCTACATGACCGGCGTGAAGATGAACAACGAGGTCATCTCGATGTC
GGCCGACACCAAGGCCAGCGACAGCGCCGGCAAGGGCTACGTCAGCGGTGCCGATACCACCTGCCCCGCCGGCAACGGCA
CCCCGGCGACCACGCTGCTGGAGCTGGCGAAGGCCGCCGGCAAGTCGGTCGGCGCGGTGACCACCACGCGCGTCACGCAT
GCCACGCCGGCCGCCACGTATTCGCACCTCTGCCACCGCGATGGCGAGAACCAGATCGCCGCGCAGGGCACGCCGGGCAA
CGCGCTGTACAACACCGCGCTGAAGGACGGCCTGGACGTGCTGCTGGGCGGCGGCCGCCGCCACTTCCTGCCGCAGGGCA
CGACCGGCAGCAAGCGCACCGACACCACCGACCTGATCGCCCAGTTCCAGGCCGCCAGCTACACCTACGTGTCGACCGGC
ACGCAGCTGGCGGCGGTCGACCCGGCCGCGACCACCAGGCTGCTCGGCCTGTTCAATCTCGACCACATGAACTATGAGCT
GGACCGCGTGAACAAGCGCGTGGACGAGCCCAGCCTGGCCGACATGACGGAGAAGGCCATCCGCATCCTGCAGAAGAACG
GCAACGGCTACTTCCTGATGGTCGAGGGCGGCCGCATCGACCACGCGCTGCACGCCACCAACGCCAAGCGCGCGCTGGAA
GACACCGTCGCCTTCGACGAGGCCATCAAGCGCGCGCTGTCGCTGGTCGACCGCGGCAACACGCTGATCGTCGTCACGGC
CGACCACGACCACACCATGACGATCAACGGCTACGCGCATCGCGGCAATCCGATCCTCGGGCTGTCCACCGACCTCAAGA
CCCGCCAGCCGCAGTTGGCCGCCGACGGCCTGCCCTACACCACCCTGGTGTTCGGCAACGGCGGCAACACGCGCAAGGCG
ACGCGCGACAACCTCGCGGCGGTCGACACCACGGCGGACGACTACTTGCAGGATGTCGGCGTGCAGCTCGGTAACCCCGG
CTCGGAAACGCACGGCGGCGGCGACGTGATGCTGTTCTCCTCCGGCCCCGGCAGCGCGCCGCTGAAAGGCACGCTGGACA
ACACGCGGGTGTTCGGCGCGGTCAGGTCGGCCATGGGCCTCTGA

Upstream 100 bases:

>100_bases
GGCCTGGGCGCGGAGCGCTTCACGGGCGTGATGGACAACACCGAGGTCTTCGGCCTCATCAAGCAGGCCGTCGGCCTGTG
ACACTCGACGGAACACTGCC

Downstream 100 bases:

>100_bases
CCCGTGCGTTTCCTTTCGCGCGCATGCCGGCATCGGGCGGCATGCGCATGTTGTTCCTTTCCGACGGTTGAGTTCCGAGC
GATGCATCCGATCCGTCTGA

Product: alkaline phosphatase signal peptide protein

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTKQSNRILRAGALALAAAAALPVLPARAAGEARNVIFFLGDGMGPTTVTAARIYKAGEAGQLTMESLKRTARIKTYSND
AQTTDSAPSMSAYMTGVKMNNEVISMSADTKASDSAGKGYVSGADTTCPAGNGTPATTLLELAKAAGKSVGAVTTTRVTH
ATPAATYSHLCHRDGENQIAAQGTPGNALYNTALKDGLDVLLGGGRRHFLPQGTTGSKRTDTTDLIAQFQAASYTYVSTG
TQLAAVDPAATTRLLGLFNLDHMNYELDRVNKRVDEPSLADMTEKAIRILQKNGNGYFLMVEGGRIDHALHATNAKRALE
DTVAFDEAIKRALSLVDRGNTLIVVTADHDHTMTINGYAHRGNPILGLSTDLKTRQPQLAADGLPYTTLVFGNGGNTRKA
TRDNLAAVDTTADDYLQDVGVQLGNPGSETHGGGDVMLFSSGPGSAPLKGTLDNTRVFGAVRSAMGL

Sequences:

>Translated_467_residues
MTKQSNRILRAGALALAAAAALPVLPARAAGEARNVIFFLGDGMGPTTVTAARIYKAGEAGQLTMESLKRTARIKTYSND
AQTTDSAPSMSAYMTGVKMNNEVISMSADTKASDSAGKGYVSGADTTCPAGNGTPATTLLELAKAAGKSVGAVTTTRVTH
ATPAATYSHLCHRDGENQIAAQGTPGNALYNTALKDGLDVLLGGGRRHFLPQGTTGSKRTDTTDLIAQFQAASYTYVSTG
TQLAAVDPAATTRLLGLFNLDHMNYELDRVNKRVDEPSLADMTEKAIRILQKNGNGYFLMVEGGRIDHALHATNAKRALE
DTVAFDEAIKRALSLVDRGNTLIVVTADHDHTMTINGYAHRGNPILGLSTDLKTRQPQLAADGLPYTTLVFGNGGNTRKA
TRDNLAAVDTTADDYLQDVGVQLGNPGSETHGGGDVMLFSSGPGSAPLKGTLDNTRVFGAVRSAMGL
>Mature_466_residues
TKQSNRILRAGALALAAAAALPVLPARAAGEARNVIFFLGDGMGPTTVTAARIYKAGEAGQLTMESLKRTARIKTYSNDA
QTTDSAPSMSAYMTGVKMNNEVISMSADTKASDSAGKGYVSGADTTCPAGNGTPATTLLELAKAAGKSVGAVTTTRVTHA
TPAATYSHLCHRDGENQIAAQGTPGNALYNTALKDGLDVLLGGGRRHFLPQGTTGSKRTDTTDLIAQFQAASYTYVSTGT
QLAAVDPAATTRLLGLFNLDHMNYELDRVNKRVDEPSLADMTEKAIRILQKNGNGYFLMVEGGRIDHALHATNAKRALED
TVAFDEAIKRALSLVDRGNTLIVVTADHDHTMTINGYAHRGNPILGLSTDLKTRQPQLAADGLPYTTLVFGNGGNTRKAT
RDNLAAVDTTADDYLQDVGVQLGNPGSETHGGGDVMLFSSGPGSAPLKGTLDNTRVFGAVRSAMGL

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=455, Percent_Identity=40, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI294660770, Length=450, Percent_Identity=39.5555555555556, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI94721246, Length=479, Percent_Identity=37.3695198329854, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI157266296, Length=479, Percent_Identity=37.3695198329854, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI157266292, Length=452, Percent_Identity=36.5044247787611, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI294660772, Length=406, Percent_Identity=38.6699507389163, Blast_Score=237, Evalue=2e-62,
Organism=Escherichia coli, GI48994877, Length=472, Percent_Identity=31.5677966101695, Blast_Score=158, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6320689, Length=368, Percent_Identity=31.7934782608696, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21355149, Length=453, Percent_Identity=38.1898454746137, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI85725200, Length=452, Percent_Identity=37.6106194690265, Blast_Score=263, Evalue=3e-70,
Organism=Drosophila melanogaster, GI85815833, Length=452, Percent_Identity=37.6106194690265, Blast_Score=263, Evalue=3e-70,
Organism=Drosophila melanogaster, GI21355981, Length=445, Percent_Identity=37.0786516853933, Blast_Score=261, Evalue=1e-69,
Organism=Drosophila melanogaster, GI21358067, Length=457, Percent_Identity=38.074398249453, Blast_Score=261, Evalue=1e-69,
Organism=Drosophila melanogaster, GI21355151, Length=451, Percent_Identity=37.6940133037694, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI19921912, Length=464, Percent_Identity=38.1465517241379, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24585213, Length=475, Percent_Identity=33.2631578947368, Blast_Score=221, Evalue=8e-58,
Organism=Drosophila melanogaster, GI18859923, Length=441, Percent_Identity=34.6938775510204, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24651554, Length=475, Percent_Identity=33.4736842105263, Blast_Score=208, Evalue=5e-54,
Organism=Drosophila melanogaster, GI24657835, Length=466, Percent_Identity=33.0472103004292, Blast_Score=205, Evalue=6e-53,
Organism=Drosophila melanogaster, GI24657842, Length=467, Percent_Identity=32.9764453961456, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24657827, Length=459, Percent_Identity=33.3333333333333, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24651556, Length=463, Percent_Identity=32.6133909287257, Blast_Score=188, Evalue=6e-48,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 48903; Mature: 48771

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQSNRILRAGALALAAAAALPVLPARAAGEARNVIFFLGDGMGPTTVTAARIYKAGEA
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHEECCCC
GQLTMESLKRTARIKTYSNDAQTTDSAPSMSAYMTGVKMNNEVISMSADTKASDSAGKGY
CCHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHEEEEEECCEEEEEECCCCCCCCCCCCE
VSGADTTCPAGNGTPATTLLELAKAAGKSVGAVTTTRVTHATPAATYSHLCHRDGENQIA
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCEE
AQGTPGNALYNTALKDGLDVLLGGGRRHFLPQGTTGSKRTDTTDLIAQFQAASYTYVSTG
ECCCCCCHHHHHHHHCCCCEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHCCEEEEECC
TQLAAVDPAATTRLLGLFNLDHMNYELDRVNKRVDEPSLADMTEKAIRILQKNGNGYFLM
CEEEEECCHHHHHHHHHEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEE
VEGGRIDHALHATNAKRALEDTVAFDEAIKRALSLVDRGNTLIVVTADHDHTMTINGYAH
EECCCCCCHHECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECEEE
RGNPILGLSTDLKTRQPQLAADGLPYTTLVFGNGGNTRKATRDNLAAVDTTADDYLQDVG
CCCEEEEECCCCCCCCCCHHCCCCCEEEEEEECCCCCCCCCCCCCEEECCCHHHHHHHHC
VQLGNPGSETHGGGDVMLFSSGPGSAPLKGTLDNTRVFGAVRSAMGL
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TKQSNRILRAGALALAAAAALPVLPARAAGEARNVIFFLGDGMGPTTVTAARIYKAGEA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHEECCCC
GQLTMESLKRTARIKTYSNDAQTTDSAPSMSAYMTGVKMNNEVISMSADTKASDSAGKGY
CCHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHEEEEEECCEEEEEECCCCCCCCCCCCE
VSGADTTCPAGNGTPATTLLELAKAAGKSVGAVTTTRVTHATPAATYSHLCHRDGENQIA
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCEE
AQGTPGNALYNTALKDGLDVLLGGGRRHFLPQGTTGSKRTDTTDLIAQFQAASYTYVSTG
ECCCCCCHHHHHHHHCCCCEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHCCEEEEECC
TQLAAVDPAATTRLLGLFNLDHMNYELDRVNKRVDEPSLADMTEKAIRILQKNGNGYFLM
CEEEEECCHHHHHHHHHEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEE
VEGGRIDHALHATNAKRALEDTVAFDEAIKRALSLVDRGNTLIVVTADHDHTMTINGYAH
EECCCCCCHHECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECEEE
RGNPILGLSTDLKTRQPQLAADGLPYTTLVFGNGGNTRKATRDNLAAVDTTADDYLQDVG
CCCEEEEECCCCCCCCCCHHCCCCCEEEEEEECCCCCCCCCCCCCEEECCCHHHHHHHHC
VQLGNPGSETHGGGDVMLFSSGPGSAPLKGTLDNTRVFGAVRSAMGL
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]