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The map label for this gene is fmt

Identifier: 17544791

GI number: 17544791

Start: 82595

End: 83578

Strand: Direct

Name: fmt

Synonym: RSc0072

Alternate gene names: 17544791

Gene position: 82595-83578 (Clockwise)

Preceding gene: 17544790

Following gene: 17544792

Centisome position: 2.22

GC content: 72.56

Gene sequence:

>984_bases
ATGACTTCCACGCTACGCGTTGCGTTCGCCGGCACGCCCGAGTTCGCGCAGATCGCCCTGGCGGCGCTCCACCAGGCCGG
CCTTCCCATCGTCGCCGTGCTGAGCCAGCCGGACCGCCCGGCCGGGCGCGGCATGCATCTGCAGGCCAGCCCGGTCAAGC
AGTACGCCGTGTCCCACGGGCTGGGGCCGGTCCTGCAGCCGCCGTCGCTGCGGCGCACCGGCAAGTATCCGCAGGAGGCG
GCCGCGGCGATCGACGCGCTGTCCGCCCAGCAGCCGGACGTGATGGTGGTGGCGGCCTACGGGCTGATCCTGCCGCAGGA
GGTGCTGGACCTGCCGCGCTTCGGCTGCATCAATATCCATGGCTCGCTGCTGCCGCGCTGGCGAGGCGCCGCGCCGATCC
ACCGCGCCATCGAAGCCGGTGATGCCGAGACCGGTATTACGCTGATGCAGATGGACGCGGGCCTGGACACGGGCGACATG
ATCGCCATGGAGCACGTGCCGATCGGCCTGACCGACACCACCGGCACGCTGCACGACACGCTGGCGGCGCTCGGCGGCCG
CATGGTGGTGGAGGCGCTCGCCAGGCTGGCGCAGGACGGCAGCCTGCCCGCCACGCCGCAGCCGGCGGAGGGCGTCACCT
ATGCCGAGAAGATCGCCAAGGAGGAGGCGGCGCTGGACTGGTCGCGGCAGTCGGCCGCGCTGCTGCGGCAGGTCCATGCC
TTCAACCCGTTTCCCGGCGCGTCGGCCGAACTGGACGGGGTGAGCATCAAGTTCTGGCAGGCCGAGGCCCTGCCCGACCG
GCCCGCCGATGCGCAGCCCGGTGCCGTGCTCGCGGCCGATGCCGATGGCGTGGTGATCGCCTGTGGCGCCGGTGCGCTGC
GTGTGACACAGTTGCAGAAGCCGGGCGGCAAGCGCCTGCCCGCGCGCGAGTTCCTGCAGGGTTTGCCGATCCGGCCGGGG
CAGCGCTTCGCTTCGCGCGCCTGA

Upstream 100 bases:

>100_bases
CGCGCCCGAACCGATCGTGCCGCTGACCGATACGCAGCACCGCGGTCGCCCATAACCCCGGCGCCGTCGGCGCGGCGCGC
TTTCCTGCCCTTTCCGATCC

Downstream 100 bases:

>100_bases
ATTTCCCGCTGTCTTTCCTGGAAGGATTGCCGCCATGAGTACGCTGGGCATCATCAACCTGCCGGTCTTCATGCTTGCCG
TGCTCCTGCTCAACGTGACG

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTSTLRVAFAGTPEFAQIALAALHQAGLPIVAVLSQPDRPAGRGMHLQASPVKQYAVSHGLGPVLQPPSLRRTGKYPQEA
AAAIDALSAQQPDVMVVAAYGLILPQEVLDLPRFGCINIHGSLLPRWRGAAPIHRAIEAGDAETGITLMQMDAGLDTGDM
IAMEHVPIGLTDTTGTLHDTLAALGGRMVVEALARLAQDGSLPATPQPAEGVTYAEKIAKEEAALDWSRQSAALLRQVHA
FNPFPGASAELDGVSIKFWQAEALPDRPADAQPGAVLAADADGVVIACGAGALRVTQLQKPGGKRLPAREFLQGLPIRPG
QRFASRA

Sequences:

>Translated_327_residues
MTSTLRVAFAGTPEFAQIALAALHQAGLPIVAVLSQPDRPAGRGMHLQASPVKQYAVSHGLGPVLQPPSLRRTGKYPQEA
AAAIDALSAQQPDVMVVAAYGLILPQEVLDLPRFGCINIHGSLLPRWRGAAPIHRAIEAGDAETGITLMQMDAGLDTGDM
IAMEHVPIGLTDTTGTLHDTLAALGGRMVVEALARLAQDGSLPATPQPAEGVTYAEKIAKEEAALDWSRQSAALLRQVHA
FNPFPGASAELDGVSIKFWQAEALPDRPADAQPGAVLAADADGVVIACGAGALRVTQLQKPGGKRLPAREFLQGLPIRPG
QRFASRA
>Mature_326_residues
TSTLRVAFAGTPEFAQIALAALHQAGLPIVAVLSQPDRPAGRGMHLQASPVKQYAVSHGLGPVLQPPSLRRTGKYPQEAA
AAIDALSAQQPDVMVVAAYGLILPQEVLDLPRFGCINIHGSLLPRWRGAAPIHRAIEAGDAETGITLMQMDAGLDTGDMI
AMEHVPIGLTDTTGTLHDTLAALGGRMVVEALARLAQDGSLPATPQPAEGVTYAEKIAKEEAALDWSRQSAALLRQVHAF
NPFPGASAELDGVSIKFWQAEALPDRPADAQPGAVLAADADGVVIACGAGALRVTQLQKPGGKRLPAREFLQGLPIRPGQ
RFASRA

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=320, Percent_Identity=29.375, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI21614513, Length=322, Percent_Identity=29.1925465838509, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI164663775, Length=243, Percent_Identity=34.156378600823, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1789683, Length=313, Percent_Identity=51.1182108626198, Blast_Score=294, Evalue=7e-81,
Organism=Escherichia coli, GI1788589, Length=310, Percent_Identity=26.7741935483871, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI133930964, Length=329, Percent_Identity=27.355623100304, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319458, Length=246, Percent_Identity=26.8292682926829, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45550868, Length=314, Percent_Identity=29.9363057324841, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI28571984, Length=236, Percent_Identity=31.7796610169492, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24585660, Length=253, Percent_Identity=25.6916996047431, Blast_Score=74, Evalue=9e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_RALSO (Q8Y3A8)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518193.1
- ProteinModelPortal:   Q8Y3A8
- SMR:   Q8Y3A8
- GeneID:   1218874
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0072
- NMPDR:   fig|267608.1.peg.72
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   PRK00005
- BioCyc:   RSOL267608:RSC0072-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34238; Mature: 34106

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTLRVAFAGTPEFAQIALAALHQAGLPIVAVLSQPDRPAGRGMHLQASPVKQYAVSHG
CCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCHHHHHHHHHC
LGPVLQPPSLRRTGKYPQEAAAAIDALSAQQPDVMVVAAYGLILPQEVLDLPRFGCINIH
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHCCHHHHCCCCCCEEEEC
GSLLPRWRGAAPIHRAIEAGDAETGITLMQMDAGLDTGDMIAMEHVPIGLTDTTGTLHDT
CCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCHHHHH
LAALGGRMVVEALARLAQDGSLPATPQPAEGVTYAEKIAKEEAALDWSRQSAALLRQVHA
HHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
FNPFPGASAELDGVSIKFWQAEALPDRPADAQPGAVLAADADGVVIACGAGALRVTQLQK
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEEECCCCEEEEEECC
PGGKRLPAREFLQGLPIRPGQRFASRA
CCCCCCCHHHHHHCCCCCCCHHHHCCC
>Mature Secondary Structure 
TSTLRVAFAGTPEFAQIALAALHQAGLPIVAVLSQPDRPAGRGMHLQASPVKQYAVSHG
CCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCHHHHHHHHHC
LGPVLQPPSLRRTGKYPQEAAAAIDALSAQQPDVMVVAAYGLILPQEVLDLPRFGCINIH
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHCCHHHHCCCCCCEEEEC
GSLLPRWRGAAPIHRAIEAGDAETGITLMQMDAGLDTGDMIAMEHVPIGLTDTTGTLHDT
CCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCHHHHH
LAALGGRMVVEALARLAQDGSLPATPQPAEGVTYAEKIAKEEAALDWSRQSAALLRQVHA
HHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
FNPFPGASAELDGVSIKFWQAEALPDRPADAQPGAVLAADADGVVIACGAGALRVTQLQK
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEEECCCCEEEEEECC
PGGKRLPAREFLQGLPIRPGQRFASRA
CCCCCCCHHHHHHCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852