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The map label for this gene is rfbD [H]

Identifier: 17232320

GI number: 17232320

Start: 5746681

End: 5747502

Strand: Reverse

Name: rfbD [H]

Synonym: all4828

Alternate gene names: 17232320

Gene position: 5747502-5746681 (Counterclockwise)

Preceding gene: 17232321

Following gene: 17232319

Centisome position: 89.61

GC content: 42.21

Gene sequence:

>822_bases
ATGAACCAAAACAAGCGAGCATTAATAACTGGGATTACTGGTCAAGATGGTTCATATCTAAGCGAATTTCTCATAGAACA
AGGTTATGAAGTTCACGGTATTATTCGCCGGACTTCCACATTCAATACAGACCGCATCGATCACATTTATGAAGATCCCC
ACCAAGAAGGAGCGCGGTTATTTCTTCACTACGGTGATTTAACCGATGGTACAACACTACGCCGAATTCTCGAAGAAGTT
CAACCAACAGAAATCTATAACTTAGGCGCTCAATCTCATGTGCGAGTAAGTTTTGATTCGCCAGAATATACAGTAGATGC
CGTAGGTATGGGAACACTACGGTTATTAGAAGCTATCCGTGACTACCAGCGACGCACAGGTATTCAAGTACGATTTTATC
AAGCAGGTTCTTCGGAAATGTATGGTTTAGTACAAGCTGTACCTCAAAGCGAAACTACACCATTTTATCCCCGTAGTCCC
TACGCTTGTGCCAAAGTTTATGCCCACTGGCAAACAGTAAATTATCGGGAATCTTACGGTTTATTTGCTTGTAATGGCAT
ACTTTTTAACCATGAATCACCTCGTCGCGGTGAAACTTTTGTCACCCGCAAAATTACAAGAGCTGTTGCCCAGATTGTAG
CCGGCAATCAGAAAAGCATCTATATGGGTAATCTTGATGCCAAGAGGGATTGGGGTTATGCCAAAGATTATGTCCGAGCT
ATGTGGTTGATGTTGCAACAAGAACAACCAGATGATTATGTAGTTGCTACCGGCGAAACCACTCAGTACGTGAATTTCTT
GAGTTGGCTTTTAGCTATGTAA

Upstream 100 bases:

>100_bases
AACGCGTAAGTTTACCGTTAGGCAATTTAGTCATTACCTGCTAGGTTGTATCAATTGACCTGCGAAAATTTCATCACCGA
GAATAAGGAATAAACAAAGC

Downstream 100 bases:

>100_bases
ATCTTAACTGGCAAGATTATGTAGAGTTTGATGAGCGTTATCTGCGTCCAGCAGAAGTAGATTTATTAATTGGTGATCCT
ACCAAGGCACAACAGAAATT

Product: GDP-D-mannose dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNQNKRALITGITGQDGSYLSEFLIEQGYEVHGIIRRTSTFNTDRIDHIYEDPHQEGARLFLHYGDLTDGTTLRRILEEV
QPTEIYNLGAQSHVRVSFDSPEYTVDAVGMGTLRLLEAIRDYQRRTGIQVRFYQAGSSEMYGLVQAVPQSETTPFYPRSP
YACAKVYAHWQTVNYRESYGLFACNGILFNHESPRRGETFVTRKITRAVAQIVAGNQKSIYMGNLDAKRDWGYAKDYVRA
MWLMLQQEQPDDYVVATGETTQYVNFLSWLLAM

Sequences:

>Translated_273_residues
MNQNKRALITGITGQDGSYLSEFLIEQGYEVHGIIRRTSTFNTDRIDHIYEDPHQEGARLFLHYGDLTDGTTLRRILEEV
QPTEIYNLGAQSHVRVSFDSPEYTVDAVGMGTLRLLEAIRDYQRRTGIQVRFYQAGSSEMYGLVQAVPQSETTPFYPRSP
YACAKVYAHWQTVNYRESYGLFACNGILFNHESPRRGETFVTRKITRAVAQIVAGNQKSIYMGNLDAKRDWGYAKDYVRA
MWLMLQQEQPDDYVVATGETTQYVNFLSWLLAM
>Mature_273_residues
MNQNKRALITGITGQDGSYLSEFLIEQGYEVHGIIRRTSTFNTDRIDHIYEDPHQEGARLFLHYGDLTDGTTLRRILEEV
QPTEIYNLGAQSHVRVSFDSPEYTVDAVGMGTLRLLEAIRDYQRRTGIQVRFYQAGSSEMYGLVQAVPQSETTPFYPRSP
YACAKVYAHWQTVNYRESYGLFACNGILFNHESPRRGETFVTRKITRAVAQIVAGNQKSIYMGNLDAKRDWGYAKDYVRA
MWLMLQQEQPDDYVVATGETTQYVNFLSWLLAM

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=265, Percent_Identity=64.9056603773585, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1788366, Length=257, Percent_Identity=69.6498054474708, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI48994969, Length=246, Percent_Identity=27.2357723577236, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788353, Length=257, Percent_Identity=25.2918287937743, Blast_Score=78, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17539424, Length=270, Percent_Identity=60.7407407407407, Blast_Score=356, Evalue=9e-99,
Organism=Caenorhabditis elegans, GI133901788, Length=270, Percent_Identity=60.7407407407407, Blast_Score=355, Evalue=9e-99,
Organism=Caenorhabditis elegans, GI133901790, Length=270, Percent_Identity=60.7407407407407, Blast_Score=355, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI133901786, Length=270, Percent_Identity=60.7407407407407, Blast_Score=355, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI17539422, Length=270, Percent_Identity=60.7407407407407, Blast_Score=355, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI17507723, Length=272, Percent_Identity=61.3970588235294, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24158427, Length=269, Percent_Identity=62.8252788104089, Blast_Score=360, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 31346; Mature: 31346

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQNKRALITGITGQDGSYLSEFLIEQGYEVHGIIRRTSTFNTDRIDHIYEDPHQEGARL
CCCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCEE
FLHYGDLTDGTTLRRILEEVQPTEIYNLGAQSHVRVSFDSPEYTVDAVGMGTLRLLEAIR
EEEECCCCCCHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCEEEEECCCHHHHHHHHHH
DYQRRTGIQVRFYQAGSSEMYGLVQAVPQSETTPFYPRSPYACAKVYAHWQTVNYRESYG
HHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCC
LFACNGILFNHESPRRGETFVTRKITRAVAQIVAGNQKSIYMGNLDAKRDWGYAKDYVRA
EEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHH
MWLMLQQEQPDDYVVATGETTQYVNFLSWLLAM
HHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNQNKRALITGITGQDGSYLSEFLIEQGYEVHGIIRRTSTFNTDRIDHIYEDPHQEGARL
CCCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCEE
FLHYGDLTDGTTLRRILEEVQPTEIYNLGAQSHVRVSFDSPEYTVDAVGMGTLRLLEAIR
EEEECCCCCCHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCEEEEECCCHHHHHHHHHH
DYQRRTGIQVRFYQAGSSEMYGLVQAVPQSETTPFYPRSPYACAKVYAHWQTVNYRESYG
HHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCC
LFACNGILFNHESPRRGETFVTRKITRAVAQIVAGNQKSIYMGNLDAKRDWGYAKDYVRA
EEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHH
MWLMLQQEQPDDYVVATGETTQYVNFLSWLLAM
HHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9098074; 10521656 [H]