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The map label for this gene is ubiF [H]

Identifier: 17232272

GI number: 17232272

Start: 5695242

End: 5696477

Strand: Reverse

Name: ubiF [H]

Synonym: all4780

Alternate gene names: 17232272

Gene position: 5696477-5695242 (Counterclockwise)

Preceding gene: 17232274

Following gene: 17232271

Centisome position: 88.82

GC content: 45.79

Gene sequence:

>1236_bases
GTGATGACTACCGATTTACTGACTAATCCTGCCCATGAGATTGTAGATGTCAAAACTACGGACTGTTGTATTGTCGGAGG
TGGCCCGGCTGGTGCGGTCTTAGCTTTATTGTTGGCGCGTCAAGGTGTTTCTGTAATATTGCTGGAAGCCCATAAAGACT
TTGACCGAGATTTTCGTGGGGATACGATTCACCCATCGGTGATGGAAATTATGGAGGAATTGGGATTAAGCGATCGCTTG
CTACAATTACCCCATGCCAAAATGCGACAAATTCGCGTAAAAACAACTGAAAATACTTTTACTTTGGCTGATTTTAGCCA
CCTAAAAACTCGCTATCCCTATATTACAATGTTGCCTCAGGTGAAATTCCTAGAGTTCATTACCCAAGAGGCTCAAAAAT
ACCCAAGCTTTAAATTAGTCATGGGTGCGAATGTGCAGGAATTAATCCAGGAGAACGGAGTAATTCAAGGTGTGCGCTAT
CGAGGCGGCGGTGGTTGGCACGAAATCAGGGCAATACTCACGGTGGGTGCAGATGGTCGTCATTCGCGGTTACGGCAATT
GGGTAATTTTGCCGCAACTGAAACCTCACCGCCAATGGATATTCTTTGGTTCCGCCTACCGCGTCAACCAGAAGATGGTG
AGGGAGGAATGGGGCGTTTTGCCCCTGGTCATATCATCGCTATGCTTGACCGTGGTAATGAATGGCAAATGGCTTATGTG
ATTCCTAAGGGAGGCTATCAGCAGTTACGGTCTACAGGTTTGGAAGCGCTGAAAAAATCTGTTGTGGAAGTCGTCCCAGA
ATTAGCAAATCGTATCCACAATTTACACGATTGGTCACAGGTAGCATTTCTCTCTGTGGAATCAAACCTTGTGCAGCGCT
GGTATCGTCCAGGATTGCTATTGATTGGTGATGCAGCTCATATCATGTCACCAGTCGCCGGAGTGGGGATTAATTATGCG
ATTCAAGATGCGGTGGTAACTGCTAATGTCCTGACTAAACCACTGCAAAATCACCATGTAAAACTTAGGGATTTAGCCAA
AGTCCAGCGTCGGCGGGAATTGCCTACACGGATTATTCAAGCATTTCAAACCTTCATTCAAAAGCGAGTATTTGCCCCTA
TACTAGCCGAGAATCTGAATTTTCAGCCCCCGATGCTGTTGCGCTTGCCAATATTGCGCGACCTACCAGCTAGATTAATT
GCTTTGGGTGTTTTTCCTGTTCATGTTCAAACTTAA

Upstream 100 bases:

>100_bases
CGTCCATGATACACATTCTCTATCGCTACGAAAAATTAAAAAAATACTCGCACCACCCTAAAGCACACTAATCTTGATTT
TAGGGCTGTCTGGAGGTGTT

Downstream 100 bases:

>100_bases
ATTCAGTATCAAGTATTTTCTCTGTCAATAAAGTGGTACAATTATACTACTCATGCAGAAATCAATGCTCTTTTAGTCAG
GCGGGAGTGGCAAACAGCAT

Product: hypothetical protein

Products: 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MMTTDLLTNPAHEIVDVKTTDCCIVGGGPAGAVLALLLARQGVSVILLEAHKDFDRDFRGDTIHPSVMEIMEELGLSDRL
LQLPHAKMRQIRVKTTENTFTLADFSHLKTRYPYITMLPQVKFLEFITQEAQKYPSFKLVMGANVQELIQENGVIQGVRY
RGGGGWHEIRAILTVGADGRHSRLRQLGNFAATETSPPMDILWFRLPRQPEDGEGGMGRFAPGHIIAMLDRGNEWQMAYV
IPKGGYQQLRSTGLEALKKSVVEVVPELANRIHNLHDWSQVAFLSVESNLVQRWYRPGLLLIGDAAHIMSPVAGVGINYA
IQDAVVTANVLTKPLQNHHVKLRDLAKVQRRRELPTRIIQAFQTFIQKRVFAPILAENLNFQPPMLLRLPILRDLPARLI
ALGVFPVHVQT

Sequences:

>Translated_411_residues
MMTTDLLTNPAHEIVDVKTTDCCIVGGGPAGAVLALLLARQGVSVILLEAHKDFDRDFRGDTIHPSVMEIMEELGLSDRL
LQLPHAKMRQIRVKTTENTFTLADFSHLKTRYPYITMLPQVKFLEFITQEAQKYPSFKLVMGANVQELIQENGVIQGVRY
RGGGGWHEIRAILTVGADGRHSRLRQLGNFAATETSPPMDILWFRLPRQPEDGEGGMGRFAPGHIIAMLDRGNEWQMAYV
IPKGGYQQLRSTGLEALKKSVVEVVPELANRIHNLHDWSQVAFLSVESNLVQRWYRPGLLLIGDAAHIMSPVAGVGINYA
IQDAVVTANVLTKPLQNHHVKLRDLAKVQRRRELPTRIIQAFQTFIQKRVFAPILAENLNFQPPMLLRLPILRDLPARLI
ALGVFPVHVQT
>Mature_411_residues
MMTTDLLTNPAHEIVDVKTTDCCIVGGGPAGAVLALLLARQGVSVILLEAHKDFDRDFRGDTIHPSVMEIMEELGLSDRL
LQLPHAKMRQIRVKTTENTFTLADFSHLKTRYPYITMLPQVKFLEFITQEAQKYPSFKLVMGANVQELIQENGVIQGVRY
RGGGGWHEIRAILTVGADGRHSRLRQLGNFAATETSPPMDILWFRLPRQPEDGEGGMGRFAPGHIIAMLDRGNEWQMAYV
IPKGGYQQLRSTGLEALKKSVVEVVPELANRIHNLHDWSQVAFLSVESNLVQRWYRPGLLLIGDAAHIMSPVAGVGINYA
IQDAVVTANVLTKPLQNHHVKLRDLAKVQRRRELPTRIIQAFQTFIQKRVFAPILAENLNFQPPMLLRLPILRDLPARLI
ALGVFPVHVQT

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Escherichia coli, GI1786883, Length=354, Percent_Identity=24.0112994350282, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 46107; Mature: 46107

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTDLLTNPAHEIVDVKTTDCCIVGGGPAGAVLALLLARQGVSVILLEAHKDFDRDFRG
CCCCHHHCCCCHHEEEEECCCEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
DTIHPSVMEIMEELGLSDRLLQLPHAKMRQIRVKTTENTFTLADFSHLKTRYPYITMLPQ
CCCCHHHHHHHHHCCCCHHHHHCCCHHHHEEEEEECCCEEEEECHHHHHHCCCEEEECCH
VKFLEFITQEAQKYPSFKLVMGANVQELIQENGVIQGVRYRGGGGWHEIRAILTVGADGR
HHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCEEEEEECCCCCHHHEEEEEEECCCCH
HSRLRQLGNFAATETSPPMDILWFRLPRQPEDGEGGMGRFAPGHIIAMLDRGNEWQMAYV
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
IPKGGYQQLRSTGLEALKKSVVEVVPELANRIHNLHDWSQVAFLSVESNLVQRWYRPGLL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEHHHHHHHHHHCCCEE
LIGDAAHIMSPVAGVGINYAIQDAVVTANVLTKPLQNHHVKLRDLAKVQRRRELPTRIIQ
EEECHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHH
AFQTFIQKRVFAPILAENLNFQPPMLLRLPILRDLPARLIALGVFPVHVQT
HHHHHHHHHHHHHHHHHCCCCCCCHHEECCHHHHHHHHHHHHEEEEEEEEC
>Mature Secondary Structure
MMTTDLLTNPAHEIVDVKTTDCCIVGGGPAGAVLALLLARQGVSVILLEAHKDFDRDFRG
CCCCHHHCCCCHHEEEEECCCEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
DTIHPSVMEIMEELGLSDRLLQLPHAKMRQIRVKTTENTFTLADFSHLKTRYPYITMLPQ
CCCCHHHHHHHHHCCCCHHHHHCCCHHHHEEEEEECCCEEEEECHHHHHHCCCEEEECCH
VKFLEFITQEAQKYPSFKLVMGANVQELIQENGVIQGVRYRGGGGWHEIRAILTVGADGR
HHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCEEEEEECCCCCHHHEEEEEEECCCCH
HSRLRQLGNFAATETSPPMDILWFRLPRQPEDGEGGMGRFAPGHIIAMLDRGNEWQMAYV
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
IPKGGYQQLRSTGLEALKKSVVEVVPELANRIHNLHDWSQVAFLSVESNLVQRWYRPGLL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEHHHHHHHHHHCCCEE
LIGDAAHIMSPVAGVGINYAIQDAVVTANVLTKPLQNHHVKLRDLAKVQRRRELPTRIIQ
EEECHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHH
AFQTFIQKRVFAPILAENLNFQPPMLLRLPILRDLPARLIALGVFPVHVQT
HHHHHHHHHHHHHHHHHCCCCCCCHHEECCHHHHHHHHHHHHEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! [C]

Specific reaction: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! = 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]