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The map label for this gene is tuf [H]

Identifier: 17231829

GI number: 17231829

Start: 5190441

End: 5191670

Strand: Reverse

Name: tuf [H]

Synonym: all4337

Alternate gene names: 17231829

Gene position: 5191670-5190441 (Counterclockwise)

Preceding gene: 17231830

Following gene: 17231828

Centisome position: 80.95

GC content: 47.32

Gene sequence:

>1230_bases
ATGGCACGCGCAAAGTTTGAAAGAACGAAACCCCACGTAAATATCGGTACGATTGGCCACGTTGACCACGGTAAAACAAC
CTTAACGGCAGCAATCACCATGACCTTGGCAGCCCTTGGTCAAGCGGTCGCTAAAGGCTACGATCAAATCGACAACGCAC
CAGAAGAAAAGGCGCGGGGTATCACCATTAACACAGCCCACGTTGAATATGAGACCGCTAACCGTCACTACGCTCACGTA
GACTGTCCGGGTCACGCTGACTATGTGAAGAATATGATCACAGGTGCGGCTCAGATGGATGGAGCAATTCTCGTAGTTGC
TGCTACCGACGGTCCTATGCCTCAAACTCGTGAACACATCCTGCTAGCAAAACAGGTAGGCGTTCCTAAGCTAGTAGTCT
TCTTAAATAAAGAAGACATGATGGAAGACGCTGAACTACTAGAACTAGTAGAACTGGAATTAAGAGAACTGTTGACCGAG
TACGAATTCGATGGTGATGATATTCCCATCGTCAGAGGTTCGGGGTTGCAAGCTCTCGAAGTTATGACCAAGAATCCTAA
GACCCAACGCGGAGAAAATCCTTGGGTAGATAAAATCTACGAATTGATGGATGCTGTAGATTCTTATATTCCCGATCCAG
AGCGGGATATAGATAAACCATTCTTGATGGCGGTAGAAGACGTGTTCTCTATCACTGGTCGTGGTACTGTTGCTACCGGT
CGGATCGAACGCGGTAAAGTCAAAGTTGGTGATGTTGTAGAACTAGTAGGTATTAGAGATACCCGCAACACAACCGTTAC
CGGGATCGAGATGTTTAAGAAGAGTCTCGATGAAGGTATGGCTGGAGACAACGCTGGTGTACTGCTACGCGGTATCCAAA
AGGCGGATATTGAACGTGGTATGGTGTTAGCCAAACCTGGTTCCATCACTCCTCACACCCAATTTGAAGGCGAAGTTTAC
GTTCTCACGGAAAAAGAAGGTGGTCGTAAAACTCCCTTCTTCGCTGGCTACCGTCCTCAATTCTATGTGCGGACAACTGA
TGTAACAGGTACCATTAAAGCCTTTACTTCTGATGAAGGTGAAACTGTAGAAATGGTAATGCCAGGCGATCGCATCAAAG
TCACTGTTGAATTAATCAACCCAATTGCGATCGAGCAAGGGATGCGCTTCGCTATTCGTGAAGGTGGCCGCACCATCGGT
GCTGGTGTCGTTTCCAAAATCGTCAAGTAG

Upstream 100 bases:

>100_bases
ATGGAGTTCAGCCACTACGAAGAGGTGCCTCGCAGCGTAGCTGAAACTATCATTGCAAAAAGCAAAGGGAACGCTTAATT
AAAAAAGGAAACGAGGATTC

Downstream 100 bases:

>100_bases
CACCTTTTTAACCTAATAAATAAGAGCAGAGATGCCGAAAGCTTCTCTGCTCTTTTATCTGAACTTACATAAGGTACTGG
GGATTAGGTATTAGGAAAAG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MARAKFERTKPHVNIGTIGHVDHGKTTLTAAITMTLAALGQAVAKGYDQIDNAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLAKQVGVPKLVVFLNKEDMMEDAELLELVELELRELLTE
YEFDGDDIPIVRGSGLQALEVMTKNPKTQRGENPWVDKIYELMDAVDSYIPDPERDIDKPFLMAVEDVFSITGRGTVATG
RIERGKVKVGDVVELVGIRDTRNTTVTGIEMFKKSLDEGMAGDNAGVLLRGIQKADIERGMVLAKPGSITPHTQFEGEVY
VLTEKEGGRKTPFFAGYRPQFYVRTTDVTGTIKAFTSDEGETVEMVMPGDRIKVTVELINPIAIEQGMRFAIREGGRTIG
AGVVSKIVK

Sequences:

>Translated_409_residues
MARAKFERTKPHVNIGTIGHVDHGKTTLTAAITMTLAALGQAVAKGYDQIDNAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLAKQVGVPKLVVFLNKEDMMEDAELLELVELELRELLTE
YEFDGDDIPIVRGSGLQALEVMTKNPKTQRGENPWVDKIYELMDAVDSYIPDPERDIDKPFLMAVEDVFSITGRGTVATG
RIERGKVKVGDVVELVGIRDTRNTTVTGIEMFKKSLDEGMAGDNAGVLLRGIQKADIERGMVLAKPGSITPHTQFEGEVY
VLTEKEGGRKTPFFAGYRPQFYVRTTDVTGTIKAFTSDEGETVEMVMPGDRIKVTVELINPIAIEQGMRFAIREGGRTIG
AGVVSKIVK
>Mature_408_residues
ARAKFERTKPHVNIGTIGHVDHGKTTLTAAITMTLAALGQAVAKGYDQIDNAPEEKARGITINTAHVEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLAKQVGVPKLVVFLNKEDMMEDAELLELVELELRELLTEY
EFDGDDIPIVRGSGLQALEVMTKNPKTQRGENPWVDKIYELMDAVDSYIPDPERDIDKPFLMAVEDVFSITGRGTVATGR
IERGKVKVGDVVELVGIRDTRNTTVTGIEMFKKSLDEGMAGDNAGVLLRGIQKADIERGMVLAKPGSITPHTQFEGEVYV
LTEKEGGRKTPFFAGYRPQFYVRTTDVTGTIKAFTSDEGETVEMVMPGDRIKVTVELINPIAIEQGMRFAIREGGRTIGA
GVVSKIVK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=409, Percent_Identity=54.7677261613692, Blast_Score=454, Evalue=1e-128,
Organism=Homo sapiens, GI223555963, Length=436, Percent_Identity=30.5045871559633, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=30.5045871559633, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI4503475, Length=460, Percent_Identity=29.7826086956522, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI4503471, Length=454, Percent_Identity=29.0748898678414, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI194018522, Length=441, Percent_Identity=27.891156462585, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI194018520, Length=441, Percent_Identity=27.891156462585, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI194097354, Length=441, Percent_Identity=27.891156462585, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI46094014, Length=441, Percent_Identity=28.1179138321995, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI54607086, Length=354, Percent_Identity=29.3785310734463, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI4503507, Length=278, Percent_Identity=26.978417266187, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=32.5757575757576, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=34.8623853211009, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=34.8623853211009, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=34.8623853211009, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=408, Percent_Identity=70.5882352941177, Blast_Score=570, Evalue=1e-164,
Organism=Escherichia coli, GI1789737, Length=408, Percent_Identity=70.5882352941177, Blast_Score=570, Evalue=1e-164,
Organism=Escherichia coli, GI2367247, Length=291, Percent_Identity=32.6460481099656, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI48994988, Length=280, Percent_Identity=30.7142857142857, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789108, Length=337, Percent_Identity=27.299703264095, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=414, Percent_Identity=49.7584541062802, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25141371, Length=400, Percent_Identity=45.25, Blast_Score=340, Evalue=8e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=452, Percent_Identity=30.7522123893805, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=452, Percent_Identity=30.7522123893805, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=27.6548672566372, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI32566303, Length=441, Percent_Identity=28.3446712018141, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=441, Percent_Identity=27.891156462585, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=441, Percent_Identity=27.891156462585, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=283, Percent_Identity=31.095406360424, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=37.9310344827586, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=247, Percent_Identity=29.9595141700405, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17565438, Length=438, Percent_Identity=23.5159817351598, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=409, Percent_Identity=61.8581907090465, Blast_Score=512, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6325337, Length=448, Percent_Identity=30.3571428571429, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=448, Percent_Identity=30.3571428571429, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=454, Percent_Identity=24.2290748898678, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=454, Percent_Identity=25.9911894273128, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6320863, Length=282, Percent_Identity=28.3687943262411, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=279, Percent_Identity=26.8817204301075, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=139, Percent_Identity=33.8129496402878, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI281363316, Length=400, Percent_Identity=57.5, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI17864358, Length=400, Percent_Identity=57.5, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI19921738, Length=399, Percent_Identity=49.1228070175439, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45553807, Length=444, Percent_Identity=29.2792792792793, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI45553816, Length=444, Percent_Identity=29.2792792792793, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24651721, Length=444, Percent_Identity=29.2792792792793, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17864154, Length=444, Percent_Identity=29.2792792792793, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=30.5122494432071, Blast_Score=161, Evalue=9e-40,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=30.5122494432071, Blast_Score=161, Evalue=9e-40,
Organism=Drosophila melanogaster, GI45550900, Length=452, Percent_Identity=28.9823008849558, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI19922690, Length=379, Percent_Identity=30.6068601583113, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI17137380, Length=441, Percent_Identity=27.437641723356, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24647048, Length=278, Percent_Identity=26.6187050359712, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24647046, Length=278, Percent_Identity=26.6187050359712, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28572034, Length=179, Percent_Identity=36.8715083798883, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28574573, Length=148, Percent_Identity=32.4324324324324, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 44812; Mature: 44681

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAKFERTKPHVNIGTIGHVDHGKTTLTAAITMTLAALGQAVAKGYDQIDNAPEEKARG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLAKQVGVPKLVVFLNKEDMMEDAELLELVELELRELLTEYEFDGDDIPIVRGSGLQALE
EEEHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEH
VMTKNPKTQRGENPWVDKIYELMDAVDSYIPDPERDIDKPFLMAVEDVFSITGRGTVATG
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHCCCCEEEEC
RIERGKVKVGDVVELVGIRDTRNTTVTGIEMFKKSLDEGMAGDNAGVLLRGIQKADIERG
CEECCCEECCCEEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHCC
MVLAKPGSITPHTQFEGEVYVLTEKEGGRKTPFFAGYRPQFYVRTTDVTGTIKAFTSDEG
EEEECCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCEEEEEEECCCEEEEEEECCCC
ETVEMVMPGDRIKVTVELINPIAIEQGMRFAIREGGRTIGAGVVSKIVK
CEEEEEECCCCEEEEEEECCCHHHHHCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
ARAKFERTKPHVNIGTIGHVDHGKTTLTAAITMTLAALGQAVAKGYDQIDNAPEEKARG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLAKQVGVPKLVVFLNKEDMMEDAELLELVELELRELLTEYEFDGDDIPIVRGSGLQALE
EEEHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEH
VMTKNPKTQRGENPWVDKIYELMDAVDSYIPDPERDIDKPFLMAVEDVFSITGRGTVATG
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHCCCCEEEEC
RIERGKVKVGDVVELVGIRDTRNTTVTGIEMFKKSLDEGMAGDNAGVLLRGIQKADIERG
CEECCCEECCCEEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHCC
MVLAKPGSITPHTQFEGEVYVLTEKEGGRKTPFFAGYRPQFYVRTTDVTGTIKAFTSDEG
EEEECCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCEEEEEEECCCEEEEEEECCCC
ETVEMVMPGDRIKVTVELINPIAIEQGMRFAIREGGRTIGAGVVSKIVK
CEEEEEECCCCEEEEEEECCCHHHHHCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA