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The map label for this gene is rne [H]

Identifier: 17231823

GI number: 17231823

Start: 5184041

End: 5186104

Strand: Direct

Name: rne [H]

Synonym: alr4331

Alternate gene names: 17231823

Gene position: 5184041-5186104 (Clockwise)

Preceding gene: 17231822

Following gene: 17231824

Centisome position: 80.83

GC content: 45.93

Gene sequence:

>2064_bases
ATGCCAAAACAAATTATTATCGCGGAGCAGCATCAAATAGCTGCTGTGTTTTCGGAAGACCAAATACAAGAACTCGTTGT
AGCCACAGGCCATCATCAAATCGGTGACATCTACTTAGGTGTAGTAGAAAATGTATTACCAGGTATTGATGCAGCCTTTG
TCAACATTGGAGACCCAGAACGTAATGGTTTTATTCATGTAACTGACTTAGGCCCTTTACGACTCAAAAGAACAGCAGCA
GCAATTACGGAATTATTAGCACCACAACAAAAAGTTCTGGTGCAGGTAATGAAAGAGCCAACGGGAACAAAAGGCCCAAG
GCTCACAGGTAATATCACTCTGCCTGGACGTTATGTGGTACTGATGCCCTATGGTAGGGGTGTAAATTTATCGAGGCGGA
TTAAGAGTGAGAGTGAACGTAACCGCTTACGGGCATTAGCAATTTTAATTAAACCTGCCGGCATGGGTTTGCTGGTGCGT
ACAGAGGCAGAAGGCAAGCCAGAAGAAGCAATCATCGAAGATTTGGAAGTTCTGCAAAAGCAATGGGAAGCCATTCAGCA
AGAAGCCCAGTCTACCCGTGCGCCAGCACTCCTGAATCGGGACGATGACTTTATCCAGCGCGTACTACGTGATATGTATG
GCGCGGATGTGAATCGGATTGTCGTCGATTCCAGTACTGGTTTGAAACGAGTCAAGCAGTACTTGCAGAACTGGAGTGGC
GGTCAGACACCCCAAGGATTGCTGATTGACCATCACCGAGATCGCTCCCCAATTTTAGAATATTTCCGGATCAATGCCGC
AATTCGAGAAGCCCTTAAACCAAGAGTAGACCTTCCTTCTGGCGGCTATATTATTATTGAACCAACAGAAGCGTTAACAG
TAATAGATGTTAACTCTGGTTCCTTTACGCGATCGGCCACAGCTAGAGAAACCGTTTTGTGGACCAACTGTGAAGCAGCA
ACAGAAATTGCTCGCCAGCTACGTCTGCGGAATATCGCTGGGGTAATCGTCGTTGATTTCATTGATATGGAATCACGTCG
TGACCAATTACAAGTTCTTGAACACTTTAATAAAGCCCTGCGCGCAGACAAAGCTCGTCCGCAAATCGCCCAGCTCACAG
AGTTAGGTTTAGTAGAACTGACTCGCAAACGGCAAGGGCAAAATATTTATGAATTGTTTGGTGATACTTGCCCCGCCTGC
GGTGGTTTAGGACATACAGTCCGCTTACCCGGAGAAACGGAGAATCGCTTACCCACTCCAGCAGCAGAAGTTCCCGAACG
TTTTGTATCTCTGCCTACACGAGAGCCTCGTTTACCAACTGCACGCACCACCGAACCACGAGAAACCTACGATGGATTTG
GTGAAGCATTTGAAAACGACTCCGACTTGGGTGCTTTAAATTTAATTAATCATCCTAGCTATCAAGAACTGAACGATAAC
AATAAACGTCGCGCCCGTACCCGTCGCAGTCGGATTGGCATCAATGGTACAAATGGCAAAGATGAACAAAGAATTACTGC
CAACCCACTAGCTTTTATCAGTGAGTCAGATTTAGACCTTGATGGAGATGTAGAATTAAGCGCTCCACCAGAGTTACCCA
CACCTAACCTGGGCAAATCAGGCTGGATTGAAAGAGCAGAACGGACTAAAGTCATTAAAACAGAGCCAGTTAAGCCAGTG
GTAGAACCACCGGAGATTAGGACTGTAGAAATGACTCCAGAAGAACAAGATATTTTTGCTCTCATGGGGATTTCTCCGCT
AATTAAATTAGAGCAGGAGGTGAAAAATCCTAAATCCGTCATTATTAACATTGTTCAACCGGGACAAACACCAACTATAC
CGACTGAAATAACTCCGGAGCCTGTCGCTAAAGTAACACCAAGTGTTGAAGTGAATACACCAAAGGTAAAGTTGGAATCT
AAGTCTGTGTCGGTAGCTGCAACCGAACCCATAAAGTTGACAGAAACGATGGAAGAATCTGAAGTGAATGCCGCTAGCAC
TGCTAACCGTCGTCGTCGTCGTCGTTCTTCCGCGTCCGACTCAGATACGGGGGAGGACAGTTAA

Upstream 100 bases:

>100_bases
CACAATTTTTATTACCAGCAGTGTCTTTGGAGGCTGGCAGGGGCGAGAGGGATAACAAACCTCCAAGCCTGTTGATACTG
CCGATTTTTGAGGAAATTGA

Downstream 100 bases:

>100_bases
GACCAATTCTGCGAAGCAGAGCCACAGATTTAAACTAGAATTTCAGACATTTTTTAGCTGCATATTAGCGCGTCTATCCA
TAGTGCATTAATAAACTATA

Product: ribonuclease E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MPKQIIIAEQHQIAAVFSEDQIQELVVATGHHQIGDIYLGVVENVLPGIDAAFVNIGDPERNGFIHVTDLGPLRLKRTAA
AITELLAPQQKVLVQVMKEPTGTKGPRLTGNITLPGRYVVLMPYGRGVNLSRRIKSESERNRLRALAILIKPAGMGLLVR
TEAEGKPEEAIIEDLEVLQKQWEAIQQEAQSTRAPALLNRDDDFIQRVLRDMYGADVNRIVVDSSTGLKRVKQYLQNWSG
GQTPQGLLIDHHRDRSPILEYFRINAAIREALKPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSATARETVLWTNCEAA
TEIARQLRLRNIAGVIVVDFIDMESRRDQLQVLEHFNKALRADKARPQIAQLTELGLVELTRKRQGQNIYELFGDTCPAC
GGLGHTVRLPGETENRLPTPAAEVPERFVSLPTREPRLPTARTTEPRETYDGFGEAFENDSDLGALNLINHPSYQELNDN
NKRRARTRRSRIGINGTNGKDEQRITANPLAFISESDLDLDGDVELSAPPELPTPNLGKSGWIERAERTKVIKTEPVKPV
VEPPEIRTVEMTPEEQDIFALMGISPLIKLEQEVKNPKSVIINIVQPGQTPTIPTEITPEPVAKVTPSVEVNTPKVKLES
KSVSVAATEPIKLTETMEESEVNAASTANRRRRRRSSASDSDTGEDS

Sequences:

>Translated_687_residues
MPKQIIIAEQHQIAAVFSEDQIQELVVATGHHQIGDIYLGVVENVLPGIDAAFVNIGDPERNGFIHVTDLGPLRLKRTAA
AITELLAPQQKVLVQVMKEPTGTKGPRLTGNITLPGRYVVLMPYGRGVNLSRRIKSESERNRLRALAILIKPAGMGLLVR
TEAEGKPEEAIIEDLEVLQKQWEAIQQEAQSTRAPALLNRDDDFIQRVLRDMYGADVNRIVVDSSTGLKRVKQYLQNWSG
GQTPQGLLIDHHRDRSPILEYFRINAAIREALKPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSATARETVLWTNCEAA
TEIARQLRLRNIAGVIVVDFIDMESRRDQLQVLEHFNKALRADKARPQIAQLTELGLVELTRKRQGQNIYELFGDTCPAC
GGLGHTVRLPGETENRLPTPAAEVPERFVSLPTREPRLPTARTTEPRETYDGFGEAFENDSDLGALNLINHPSYQELNDN
NKRRARTRRSRIGINGTNGKDEQRITANPLAFISESDLDLDGDVELSAPPELPTPNLGKSGWIERAERTKVIKTEPVKPV
VEPPEIRTVEMTPEEQDIFALMGISPLIKLEQEVKNPKSVIINIVQPGQTPTIPTEITPEPVAKVTPSVEVNTPKVKLES
KSVSVAATEPIKLTETMEESEVNAASTANRRRRRRSSASDSDTGEDS
>Mature_686_residues
PKQIIIAEQHQIAAVFSEDQIQELVVATGHHQIGDIYLGVVENVLPGIDAAFVNIGDPERNGFIHVTDLGPLRLKRTAAA
ITELLAPQQKVLVQVMKEPTGTKGPRLTGNITLPGRYVVLMPYGRGVNLSRRIKSESERNRLRALAILIKPAGMGLLVRT
EAEGKPEEAIIEDLEVLQKQWEAIQQEAQSTRAPALLNRDDDFIQRVLRDMYGADVNRIVVDSSTGLKRVKQYLQNWSGG
QTPQGLLIDHHRDRSPILEYFRINAAIREALKPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSATARETVLWTNCEAAT
EIARQLRLRNIAGVIVVDFIDMESRRDQLQVLEHFNKALRADKARPQIAQLTELGLVELTRKRQGQNIYELFGDTCPACG
GLGHTVRLPGETENRLPTPAAEVPERFVSLPTREPRLPTARTTEPRETYDGFGEAFENDSDLGALNLINHPSYQELNDNN
KRRARTRRSRIGINGTNGKDEQRITANPLAFISESDLDLDGDVELSAPPELPTPNLGKSGWIERAERTKVIKTEPVKPVV
EPPEIRTVEMTPEEQDIFALMGISPLIKLEQEVKNPKSVIINIVQPGQTPTIPTEITPEPVAKVTPSVEVNTPKVKLESK
SVSVAATEPIKLTETMEESEVNAASTANRRRRRRSSASDSDTGEDS

Specific function: Matures 5s Rrna From Its Precursors From All The Rrna Genes. It Also Cleaves RNA I, A Molecule That Controls The Replication Of Cole1 Plasmid DNA. It Is The Major Endoribonuclease Participating In Mrna Turnover In E. coli. It Initiates The Decay Of Rnas B

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=386, Percent_Identity=39.6373056994819, Blast_Score=254, Evalue=2e-68,
Organism=Escherichia coli, GI87082235, Length=379, Percent_Identity=38.2585751978892, Blast_Score=246, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 76170; Mature: 76038

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50126 S1 ; PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKQIIIAEQHQIAAVFSEDQIQELVVATGHHQIGDIYLGVVENVLPGIDAAFVNIGDPE
CCCCEEEECCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCC
RNGFIHVTDLGPLRLKRTAAAITELLAPQQKVLVQVMKEPTGTKGPRLTGNITLPGRYVV
CCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LMPYGRGVNLSRRIKSESERNRLRALAILIKPAGMGLLVRTEAEGKPEEAIIEDLEVLQK
EEECCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHH
QWEAIQQEAQSTRAPALLNRDDDFIQRVLRDMYGADVNRIVVDSSTGLKRVKQYLQNWSG
HHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCC
GQTPQGLLIDHHRDRSPILEYFRINAAIREALKPRVDLPSGGYIIIEPTEALTVIDVNSG
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEECCC
SFTRSATARETVLWTNCEAATEIARQLRLRNIAGVIVVDFIDMESRRDQLQVLEHFNKAL
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHH
RADKARPQIAQLTELGLVELTRKRQGQNIYELFGDTCPACGGLGHTVRLPGETENRLPTP
HHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCC
AAEVPERFVSLPTREPRLPTARTTEPRETYDGFGEAFENDSDLGALNLINHPSYQELNDN
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCHHHCCCC
NKRRARTRRSRIGINGTNGKDEQRITANPLAFISESDLDLDGDVELSAPPELPTPNLGKS
CHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEECCCCCCCCCEEECCCCCCCCCCCCCC
GWIERAERTKVIKTEPVKPVVEPPEIRTVEMTPEEQDIFALMGISPLIKLEQEVKNPKSV
HHHHHHHHCCEEECCCCCCCCCCCCEEEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCE
IINIVQPGQTPTIPTEITPEPVAKVTPSVEVNTPKVKLESKSVSVAATEPIKLTETMEES
EEEEECCCCCCCCCCCCCCCCHHHCCCCEEECCCEEEEECCEEEEEECCCEEHHHHHHHH
EVNAASTANRRRRRRSSASDSDTGEDS
HCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PKQIIIAEQHQIAAVFSEDQIQELVVATGHHQIGDIYLGVVENVLPGIDAAFVNIGDPE
CCCEEEECCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCC
RNGFIHVTDLGPLRLKRTAAAITELLAPQQKVLVQVMKEPTGTKGPRLTGNITLPGRYVV
CCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LMPYGRGVNLSRRIKSESERNRLRALAILIKPAGMGLLVRTEAEGKPEEAIIEDLEVLQK
EEECCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHH
QWEAIQQEAQSTRAPALLNRDDDFIQRVLRDMYGADVNRIVVDSSTGLKRVKQYLQNWSG
HHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCC
GQTPQGLLIDHHRDRSPILEYFRINAAIREALKPRVDLPSGGYIIIEPTEALTVIDVNSG
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEECCC
SFTRSATARETVLWTNCEAATEIARQLRLRNIAGVIVVDFIDMESRRDQLQVLEHFNKAL
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHH
RADKARPQIAQLTELGLVELTRKRQGQNIYELFGDTCPACGGLGHTVRLPGETENRLPTP
HHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCC
AAEVPERFVSLPTREPRLPTARTTEPRETYDGFGEAFENDSDLGALNLINHPSYQELNDN
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCHHHCCCC
NKRRARTRRSRIGINGTNGKDEQRITANPLAFISESDLDLDGDVELSAPPELPTPNLGKS
CHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEECCCCCCCCCEEECCCCCCCCCCCCCC
GWIERAERTKVIKTEPVKPVVEPPEIRTVEMTPEEQDIFALMGISPLIKLEQEVKNPKSV
HHHHHHHHCCEEECCCCCCCCCCCCEEEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCE
IINIVQPGQTPTIPTEITPEPVAKVTPSVEVNTPKVKLESKSVSVAATEPIKLTETMEES
EEEEECCCCCCCCCCCCCCCCHHHCCCCEEECCCEEEEECCEEEEEECCCEEHHHHHHHH
EVNAASTANRRRRRRSSASDSDTGEDS
HCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]