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The map label for this gene is ndhF [H]

Identifier: 17231648

GI number: 17231648

Start: 4999616

End: 5001478

Strand: Direct

Name: ndhF [H]

Synonym: alr4156

Alternate gene names: 17231648

Gene position: 4999616-5001478 (Clockwise)

Preceding gene: 17231646

Following gene: 17231649

Centisome position: 77.95

GC content: 42.46

Gene sequence:

>1863_bases
ATGGCTCAGTTTCTCCTTGAGACTGTTTGGCTAGTCCCTTTGTATGCTCTAATTGGCGGTCTGTTAGCCATACCTTGGTC
GCCGGGGATCATTCGCAAAACGGGGCCAAGACCGGCTGGTTATGTAAATTTAATAATGACATTTTTGGCGTTGGTTCATA
GTGCGATCGCCTTACAAGCAACTTGGAGTCATCCACCCCAAGAAGTATTTCTTCCTTGGCTATCTACTGCTGGTTTAGAC
CTCACCATTGCCATAGAAATTTCTTCTATAAGTGTAGGGGCAATGGTAGTCATTACTGGCTTAAATTTCCTTGCCCAAAT
TTTTGCTATTGGCTACATGGAAATGGATTGGGGGTTGGGACGCTTTTATTCATTGCTGGGATTATTTGAAGCAGGGTTAT
GCGCTTTAGTCCTATGCAATAATCTATTTTTTAGTTATGTAATTCTGGAAATCCTCACATTAGGAACTTACTTATTAGTT
GGTTTATGGTTTAGTCAACCATTAGTAGTCAGTGGCGCGAGAGATGCTTTCTTAACCAAGCGAGTAGGAGACTTGTTCCT
GCTAATGGGTGTATTGGGATTATGGCCGTTAGCGGGAACTTGGAATTATCCAGAGCTAGCCCAATGGGCGCAAACAGCGA
ACGTAGACCCCACAATTATTACACTAGTGGGTTTAGCCTTAGTTGCTGGGCCAATGGGTAAGTGCGCCCAATTCCCCTTA
CATCTGTGGTTAGATGAAGCGATGGAAGGCCCGATGCCGAGTACTATTTTACGGAACTCGGTAGTAGTTGCCAGTGGAGC
ATGGGTACTGATTAAACTCCAACCAGTGTTAACTCTATCGCCTGTAGTTTCCTCATTTATTGTGGCAATTGGTGCAGTCA
CAGCAATTGGTGGTTCTTTAATTGCGATCGCCCAAATTGATGTTAAACGCTGCTTATCATATTCTGTTAGCGCTTACATG
GGTTTGGTATTTATTGCTGTAGGCGCACGTCAAGATGAAGCTGCACTGTTATTGGTACTCACCCATGCAGTATCGGCGGC
GTTATTGGTAATGAGTACTGGTGGGATTATTTGGAATAGCATTACCCAAGATGTGACTCAACTAGGTGGACTGTGGTCAC
GTCGTCCTATTTCTGGGTTAGCTTTCATTGTTGGGACATTGGGCTTAATTGGATTTCCCCCTCTAGGCGGCTTTTGGGCA
TTACTGAAATTAGCTGATGGCTTATGGGGAACTCACCCTTGGTTAGTAGGAATTGTCATAGCTGTCAATGCTTTAACCGC
TTTCAGTTTGGTGAGAGAATTTGGTTTAATTTTTGGTGGTAAAGCTAAACAGATGACTGAGCGATCGCCAGAAGTTCATT
GGCCGATGGTATTACCAATGATGATTTTATTTGGCTTTGTTCTTCACCTGCCTTTAATTTTGCAAGCCTTATCACTTTTA
CCTGATTGGGCAATCTTAAATAAAGATGTCGTCTTACTACTAATTTGGTCAACTATTTTCGGTTGTAGCATTAGCAGCGT
CATTTATTTAAGTAACATCATTCCTAAACCAATTCGTCTGCCTTGGAAAGGTTTACAAGACCTATTGGCATACGATTTTT
ATACCCCCAATCTGTACCGAATCACCATTATTTTCAGCGTTGCCCAACTTTCTAAATTTGCCGATATGATTGACCGTTTC
GTAGTTGATGGTATCGTCAATTTTGTTGGTCTGTTCTCACTGTTAGGTGGTGAAGGTCTAAAATATAGTACTTCTGGACA
AACTCAATTTTATGCCTTGACTGTTCTCTTAGGTGTAGGTGTTTTAGGGGCTTGGGTGACTTGGCCTTTCTGGGGATTCC
AGTTTTTGGATTTGATGTTTTAA

Upstream 100 bases:

>100_bases
CCTTATTTACAATAGCTGCAAGATAGCTGTATTACTTCGGATTTACACAAGTTAACTCCGAAATACTTTAATTTTTTGTG
ACAAATCTAGAGGTAAGTTC

Downstream 100 bases:

>100_bases
GTAATTTAGTCAGTTGTCAGTAGTCAGGTGTTAGGAGCAAGAATTAACACCTGGCTACTAAAAAATAAAATATTCTACTT
ACCCAAATATTATGCTTAGT

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 620; Mature: 619

Protein sequence:

>620_residues
MAQFLLETVWLVPLYALIGGLLAIPWSPGIIRKTGPRPAGYVNLIMTFLALVHSAIALQATWSHPPQEVFLPWLSTAGLD
LTIAIEISSISVGAMVVITGLNFLAQIFAIGYMEMDWGLGRFYSLLGLFEAGLCALVLCNNLFFSYVILEILTLGTYLLV
GLWFSQPLVVSGARDAFLTKRVGDLFLLMGVLGLWPLAGTWNYPELAQWAQTANVDPTIITLVGLALVAGPMGKCAQFPL
HLWLDEAMEGPMPSTILRNSVVVASGAWVLIKLQPVLTLSPVVSSFIVAIGAVTAIGGSLIAIAQIDVKRCLSYSVSAYM
GLVFIAVGARQDEAALLLVLTHAVSAALLVMSTGGIIWNSITQDVTQLGGLWSRRPISGLAFIVGTLGLIGFPPLGGFWA
LLKLADGLWGTHPWLVGIVIAVNALTAFSLVREFGLIFGGKAKQMTERSPEVHWPMVLPMMILFGFVLHLPLILQALSLL
PDWAILNKDVVLLLIWSTIFGCSISSVIYLSNIIPKPIRLPWKGLQDLLAYDFYTPNLYRITIIFSVAQLSKFADMIDRF
VVDGIVNFVGLFSLLGGEGLKYSTSGQTQFYALTVLLGVGVLGAWVTWPFWGFQFLDLMF

Sequences:

>Translated_620_residues
MAQFLLETVWLVPLYALIGGLLAIPWSPGIIRKTGPRPAGYVNLIMTFLALVHSAIALQATWSHPPQEVFLPWLSTAGLD
LTIAIEISSISVGAMVVITGLNFLAQIFAIGYMEMDWGLGRFYSLLGLFEAGLCALVLCNNLFFSYVILEILTLGTYLLV
GLWFSQPLVVSGARDAFLTKRVGDLFLLMGVLGLWPLAGTWNYPELAQWAQTANVDPTIITLVGLALVAGPMGKCAQFPL
HLWLDEAMEGPMPSTILRNSVVVASGAWVLIKLQPVLTLSPVVSSFIVAIGAVTAIGGSLIAIAQIDVKRCLSYSVSAYM
GLVFIAVGARQDEAALLLVLTHAVSAALLVMSTGGIIWNSITQDVTQLGGLWSRRPISGLAFIVGTLGLIGFPPLGGFWA
LLKLADGLWGTHPWLVGIVIAVNALTAFSLVREFGLIFGGKAKQMTERSPEVHWPMVLPMMILFGFVLHLPLILQALSLL
PDWAILNKDVVLLLIWSTIFGCSISSVIYLSNIIPKPIRLPWKGLQDLLAYDFYTPNLYRITIIFSVAQLSKFADMIDRF
VVDGIVNFVGLFSLLGGEGLKYSTSGQTQFYALTVLLGVGVLGAWVTWPFWGFQFLDLMF
>Mature_619_residues
AQFLLETVWLVPLYALIGGLLAIPWSPGIIRKTGPRPAGYVNLIMTFLALVHSAIALQATWSHPPQEVFLPWLSTAGLDL
TIAIEISSISVGAMVVITGLNFLAQIFAIGYMEMDWGLGRFYSLLGLFEAGLCALVLCNNLFFSYVILEILTLGTYLLVG
LWFSQPLVVSGARDAFLTKRVGDLFLLMGVLGLWPLAGTWNYPELAQWAQTANVDPTIITLVGLALVAGPMGKCAQFPLH
LWLDEAMEGPMPSTILRNSVVVASGAWVLIKLQPVLTLSPVVSSFIVAIGAVTAIGGSLIAIAQIDVKRCLSYSVSAYMG
LVFIAVGARQDEAALLLVLTHAVSAALLVMSTGGIIWNSITQDVTQLGGLWSRRPISGLAFIVGTLGLIGFPPLGGFWAL
LKLADGLWGTHPWLVGIVIAVNALTAFSLVREFGLIFGGKAKQMTERSPEVHWPMVLPMMILFGFVLHLPLILQALSLLP
DWAILNKDVVLLLIWSTIFGCSISSVIYLSNIIPKPIRLPWKGLQDLLAYDFYTPNLYRITIIFSVAQLSKFADMIDRFV
VDGIVNFVGLFSLLGGEGLKYSTSGQTQFYALTVLLGVGVLGAWVTWPFWGFQFLDLMF

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=402, Percent_Identity=28.3582089552239, Blast_Score=162, Evalue=7e-40,
Organism=Escherichia coli, GI1788614, Length=423, Percent_Identity=29.7872340425532, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1788831, Length=397, Percent_Identity=26.1964735516373, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788827, Length=447, Percent_Identity=25.2796420581655, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788613, Length=346, Percent_Identity=26.0115606936416, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788829, Length=397, Percent_Identity=24.1813602015113, Blast_Score=90, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 67363; Mature: 67232

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFLLETVWLVPLYALIGGLLAIPWSPGIIRKTGPRPAGYVNLIMTFLALVHSAIALQA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEE
TWSHPPQEVFLPWLSTAGLDLTIAIEISSISVGAMVVITGLNFLAQIFAIGYMEMDWGLG
CCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
RFYSLLGLFEAGLCALVLCNNLFFSYVILEILTLGTYLLVGLWFSQPLVVSGARDAFLTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHH
RVGDLFLLMGVLGLWPLAGTWNYPELAQWAQTANVDPTIITLVGLALVAGPMGKCAQFPL
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCHH
HLWLDEAMEGPMPSTILRNSVVVASGAWVLIKLQPVLTLSPVVSSFIVAIGAVTAIGGSL
HHHHHHHHCCCCCHHHHHCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCE
IAIAQIDVKRCLSYSVSAYMGLVFIAVGARQDEAALLLVLTHAVSAALLVMSTGGIIWNS
EEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
ITQDVTQLGGLWSRRPISGLAFIVGTLGLIGFPPLGGFWALLKLADGLWGTHPWLVGIVI
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
AVNALTAFSLVREFGLIFGGKAKQMTERSPEVHWPMVLPMMILFGFVLHLPLILQALSLL
HHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PDWAILNKDVVLLLIWSTIFGCSISSVIYLSNIIPKPIRLPWKGLQDLLAYDFYTPNLYR
CCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEE
ITIIFSVAQLSKFADMIDRFVVDGIVNFVGLFSLLGGEGLKYSTSGQTQFYALTVLLGVG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHH
VLGAWVTWPFWGFQFLDLMF
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQFLLETVWLVPLYALIGGLLAIPWSPGIIRKTGPRPAGYVNLIMTFLALVHSAIALQA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEE
TWSHPPQEVFLPWLSTAGLDLTIAIEISSISVGAMVVITGLNFLAQIFAIGYMEMDWGLG
CCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
RFYSLLGLFEAGLCALVLCNNLFFSYVILEILTLGTYLLVGLWFSQPLVVSGARDAFLTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHH
RVGDLFLLMGVLGLWPLAGTWNYPELAQWAQTANVDPTIITLVGLALVAGPMGKCAQFPL
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCHH
HLWLDEAMEGPMPSTILRNSVVVASGAWVLIKLQPVLTLSPVVSSFIVAIGAVTAIGGSL
HHHHHHHHCCCCCHHHHHCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCE
IAIAQIDVKRCLSYSVSAYMGLVFIAVGARQDEAALLLVLTHAVSAALLVMSTGGIIWNS
EEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
ITQDVTQLGGLWSRRPISGLAFIVGTLGLIGFPPLGGFWALLKLADGLWGTHPWLVGIVI
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
AVNALTAFSLVREFGLIFGGKAKQMTERSPEVHWPMVLPMMILFGFVLHLPLILQALSLL
HHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PDWAILNKDVVLLLIWSTIFGCSISSVIYLSNIIPKPIRLPWKGLQDLLAYDFYTPNLYR
CCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEE
ITIIFSVAQLSKFADMIDRFVVDGIVNFVGLFSLLGGEGLKYSTSGQTQFYALTVLLGVG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHH
VLGAWVTWPFWGFQFLDLMF
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]