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The map label for this gene is proA [C]

Identifier: 17231487

GI number: 17231487

Start: 4813428

End: 4814693

Strand: Direct

Name: proA [C]

Synonym: alr3995

Alternate gene names: 17231487

Gene position: 4813428-4814693 (Clockwise)

Preceding gene: 17231486

Following gene: 17231488

Centisome position: 75.05

GC content: 44.94

Gene sequence:

>1266_bases
ATGACCGTTGCAGCTTTAGATGATTGTCCCGAACCCATGAAAAGCGCCAAACGCGCTTTTCAAGCTTCCCTGAAGTTAGG
TACTACCAAGGGAATAGATCGTAGTCGCGCTGTTTTGGCAATGGCACAGGCGCTAGAAAACGCATTTGACGACATTTTAG
AAGCCAATACCTTGGATCTAGAAGCTAGTCGAGAAATGGCAGTGCCAGAACTGATTTTGGACTGGCTGAAATTAACTCCC
ACCAGGTTAGAGATTACAGTAGAAATTCTGCAAAGGTTGGGAGAAATATCAGACCCATTACGCCGGGTAAGAAACGCCGA
CTATCAGCCAGAAGATTCTCAGAGTTATTCTCAGTTAATGCCTTTGGGAGTAATTGGATTTATTTATGAAGCGTTTCCCG
ATTTAGGAGCGATCGCCGCAGGTTTGTGTATTAAAACCGGTAATAGTATTATCCTGAAGGGTAGTACAGAAGCTAGTCAT
TCTAATGCGGCGATCGTAGAAGCATTACAACACGCGATCGTCGAAGTTGGACTACCATCAGGCTGTATAGAACTTGTCAC
TACAGAACATGGCGGTTCCCTGCGGGATTTAATCACCCAAGACCAATATATCAACTTGGTAATTCCCTACGGACGTTCCA
GTTTAGTACAGCAGGTAATGCGCCAAGCGACTTGCCCGGTGTTAAAGTCAGCAATGGGTAACTGTTACCTCTACTGGTCG
TTAAATAGCAGCTTAGACATGGTACGTTGGATGATTCTCGATAGCCATGAAAGCGAACCAGATCCCGTCAACGCTATTGA
GAAAGTCTTAATTCATCGTCAAGCCATGCCTTCATCTTTAGCTGTTTTGTGGAATAGCCTCAAAGAGAAAGGGTTTGAAA
TTAAAGGCGACGGGGAACTAGTAGAAGCCTTTCCCCAGTTGCAGCTAGCAAAAGATAACGAATGGGGCAACGCTTATTTA
ACTAAGACAGTGGCATTTAAACTGGTAGATAGTTTGGATGGAGCGATCGCCTGGATAAATCAATACAGTAGCGGTCATGC
TGACTCCATCGCCACTGAGTCCTATCAAGAAAGTCGCCAATTTGCCTTAGGAGTTAACAGCGCTGCCACCTATATCAACA
CATCCCCACGCTTCTCTCGTAACCCTTCACGGGGTGATTCTGTGTTTTTAGGTATGTCCAATCAAAAAGGTCATCGCCGG
GGGTTTATCAGCCTAGAAACTTTGACTACCCTCAAACACATTATTCAGGGGAATGGGAGATTTTAA

Upstream 100 bases:

>100_bases
CTGGCCAATGGTTCTAGTATCATCGCTTGGGCAATGGTGGCAGTATTGTTGGCTATTTTGCCAGCACTCATGGTCTGGCT
TACTTACAGACGATAACTCA

Downstream 100 bases:

>100_bases
GTTTGGTAATGGGTAATAATGTTGTTTTCCCTTCTGCTTCCCTGCCTCTTAATAGTCCTTACACCAGGCGATCGCAGGCC
ATGATAAGTTGATCTAGTAC

Product: gamma-glutamyl phosphate reductase

Products: L-glutamyl 5-phosphate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTVAALDDCPEPMKSAKRAFQASLKLGTTKGIDRSRAVLAMAQALENAFDDILEANTLDLEASREMAVPELILDWLKLTP
TRLEITVEILQRLGEISDPLRRVRNADYQPEDSQSYSQLMPLGVIGFIYEAFPDLGAIAAGLCIKTGNSIILKGSTEASH
SNAAIVEALQHAIVEVGLPSGCIELVTTEHGGSLRDLITQDQYINLVIPYGRSSLVQQVMRQATCPVLKSAMGNCYLYWS
LNSSLDMVRWMILDSHESEPDPVNAIEKVLIHRQAMPSSLAVLWNSLKEKGFEIKGDGELVEAFPQLQLAKDNEWGNAYL
TKTVAFKLVDSLDGAIAWINQYSSGHADSIATESYQESRQFALGVNSAATYINTSPRFSRNPSRGDSVFLGMSNQKGHRR
GFISLETLTTLKHIIQGNGRF

Sequences:

>Translated_421_residues
MTVAALDDCPEPMKSAKRAFQASLKLGTTKGIDRSRAVLAMAQALENAFDDILEANTLDLEASREMAVPELILDWLKLTP
TRLEITVEILQRLGEISDPLRRVRNADYQPEDSQSYSQLMPLGVIGFIYEAFPDLGAIAAGLCIKTGNSIILKGSTEASH
SNAAIVEALQHAIVEVGLPSGCIELVTTEHGGSLRDLITQDQYINLVIPYGRSSLVQQVMRQATCPVLKSAMGNCYLYWS
LNSSLDMVRWMILDSHESEPDPVNAIEKVLIHRQAMPSSLAVLWNSLKEKGFEIKGDGELVEAFPQLQLAKDNEWGNAYL
TKTVAFKLVDSLDGAIAWINQYSSGHADSIATESYQESRQFALGVNSAATYINTSPRFSRNPSRGDSVFLGMSNQKGHRR
GFISLETLTTLKHIIQGNGRF
>Mature_420_residues
TVAALDDCPEPMKSAKRAFQASLKLGTTKGIDRSRAVLAMAQALENAFDDILEANTLDLEASREMAVPELILDWLKLTPT
RLEITVEILQRLGEISDPLRRVRNADYQPEDSQSYSQLMPLGVIGFIYEAFPDLGAIAAGLCIKTGNSIILKGSTEASHS
NAAIVEALQHAIVEVGLPSGCIELVTTEHGGSLRDLITQDQYINLVIPYGRSSLVQQVMRQATCPVLKSAMGNCYLYWSL
NSSLDMVRWMILDSHESEPDPVNAIEKVLIHRQAMPSSLAVLWNSLKEKGFEIKGDGELVEAFPQLQLAKDNEWGNAYLT
KTVAFKLVDSLDGAIAWINQYSSGHADSIATESYQESRQFALGVNSAATYINTSPRFSRNPSRGDSVFLGMSNQKGHRRG
FISLETLTTLKHIIQGNGRF

Specific function: Catalyzes The Nadph Dependent Reduction Of L-Gamma- Glutamyl 5-Phosphate Into L-Glutamate 5-Semialdehyde And Phosphate. The Product Spontaneously Undergoes Cyclization To Form 1-Pyrroline-5-Carboxylate. [C]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=400, Percent_Identity=30, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI21361368, Length=400, Percent_Identity=30, Blast_Score=149, Evalue=5e-36,
Organism=Escherichia coli, GI1786438, Length=404, Percent_Identity=30.6930693069307, Blast_Score=209, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17569967, Length=413, Percent_Identity=27.3607748184019, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17569969, Length=413, Percent_Identity=27.3607748184019, Blast_Score=132, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6324899, Length=426, Percent_Identity=30.0469483568075, Blast_Score=176, Evalue=5e-45,
Organism=Drosophila melanogaster, GI21357643, Length=394, Percent_Identity=32.2335025380711, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: 1.2.1.41

Molecular weight: Translated: 46292; Mature: 46161

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAALDDCPEPMKSAKRAFQASLKLGTTKGIDRSRAVLAMAQALENAFDDILEANTLDL
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
EASREMAVPELILDWLKLTPTRLEITVEILQRLGEISDPLRRVRNADYQPEDSQSYSQLM
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
PLGVIGFIYEAFPDLGAIAAGLCIKTGNSIILKGSTEASHSNAAIVEALQHAIVEVGLPS
HHHHHHHHHHHHCCHHHHHHHHHEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCC
GCIELVTTEHGGSLRDLITQDQYINLVIPYGRSSLVQQVMRQATCPVLKSAMGNCYLYWS
CCEEEEEECCCCHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHHCCEEEEEE
LNSSLDMVRWMILDSHESEPDPVNAIEKVLIHRQAMPSSLAVLWNSLKEKGFEIKGDGEL
ECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEECCCCHH
VEAFPQLQLAKDNEWGNAYLTKTVAFKLVDSLDGAIAWINQYSSGHADSIATESYQESRQ
HHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHH
FALGVNSAATYINTSPRFSRNPSRGDSVFLGMSNQKGHRRGFISLETLTTLKHIIQGNGR
HEEECCCHHHEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHHHCCCCC
F
C
>Mature Secondary Structure 
TVAALDDCPEPMKSAKRAFQASLKLGTTKGIDRSRAVLAMAQALENAFDDILEANTLDL
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
EASREMAVPELILDWLKLTPTRLEITVEILQRLGEISDPLRRVRNADYQPEDSQSYSQLM
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
PLGVIGFIYEAFPDLGAIAAGLCIKTGNSIILKGSTEASHSNAAIVEALQHAIVEVGLPS
HHHHHHHHHHHHCCHHHHHHHHHEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCC
GCIELVTTEHGGSLRDLITQDQYINLVIPYGRSSLVQQVMRQATCPVLKSAMGNCYLYWS
CCEEEEEECCCCHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHHCCEEEEEE
LNSSLDMVRWMILDSHESEPDPVNAIEKVLIHRQAMPSSLAVLWNSLKEKGFEIKGDGEL
ECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEECCCCHH
VEAFPQLQLAKDNEWGNAYLTKTVAFKLVDSLDGAIAWINQYSSGHADSIATESYQESRQ
HHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHH
FALGVNSAATYINTSPRFSRNPSRGDSVFLGMSNQKGHRRGFISLETLTTLKHIIQGNGR
HEEECCCHHHEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHHHCCCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 2.5 {DL-pyrroline-5-carboxylic} 11 {phosphate}} 0.18 {NADP+}} 0.05 {NADP+}} 2.2 {glutamic} [C]

Substrates: L-glutamate 5-semialdehyde; phosphate; NADP+

Specific reaction: L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: 3-(Phosphonoacetyl amido)-L-alanine; Ba2+; Co2+; Cu2+; Iodoacetamide; Iodoacetate; NADPH; Ni2+; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]