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The map label for this gene is rpfG [H]

Identifier: 17231412

GI number: 17231412

Start: 4727147

End: 4728391

Strand: Direct

Name: rpfG [H]

Synonym: alr3920

Alternate gene names: 17231412

Gene position: 4727147-4728391 (Clockwise)

Preceding gene: 17231411

Following gene: 17231413

Centisome position: 73.7

GC content: 42.89

Gene sequence:

>1245_bases
GTGATTAAAAGGAGTGGTGATAATACAACCTCTACTGAACATAATGTTAGTGGGTCAGACCCCCCAAACAAATTAAATAG
TATTAGTTCAAATCGCGTTTCCGGAGTACAAAACGCCAAAGATAGTTCCTTAGCATTGTCCAAAGAAGATTTTCTGGTTA
GTGAAAGCACGCAAAGTACTACTTGGCTGACCTGTGGGATTAATTCTGGTTACAACTCTTTAATCTCTGGAACTTTACAA
GCCCAAATTTCTCAAGTGAAAGGCTTTGATGCAGAATCGCCAAAAGTTTTAGTAGTCGATGATCACAGTGCTAGTCGGAT
GACGGCTGCTGCTCTTTTGGGTATGGAAGGCTACGCAGTAATTGAAGCAGATAGTGGTGCTACGGCGATAAAACTGGTAG
GACAACAACAACCTGATCTGATCTTGCTGGATGTAATGATGCCGGAAATGGACGGGTTTGAAGTATGCCAATTACTCAAG
CAAGATGAACATACTCGATTGATCCCCGTAATTTTTATTACGGCCTTGAATGATAGGCGATCGCGCATCCGAGGTATTGA
AGTAGGTGCAGATGATTTCTTAAGTAAACCTTTTGACCGAGTGGAATTAGTAGCGCGTGTCAAATCTTTGGTGCAACAAA
AGCGTTTGAATGAAGATTTAGACCATGCAGCACAAGTACTATTTTCCATAGCTAGCGCTATTGAAAGTCGTGATCCCAAT
ACTGGCGACCACTGCGAACGCTTAGTTACCTTAGGACAAGCCTTTGGTGAGTACCTCACTCTTTCGCGCCACCAAATTCG
AGATTTGATGTGGGGTGGCTATCTTCACGATATCGGCAAAGTAGGAATTCCCGATGCTGTGTTGCTAAAAACAGGCAAAC
TTACCCCTGAAGATTGGGTAATTATGAAGCAACACGTTTTAATCGGCGAAAAAATCTGTCAACCTCTACGCAGTATGCAG
GGTGTAGTTCCCATCATTCGTCATCACCACGAACGTTGGGATGGTTCAGGTTATCCTGATGGACTCAAAGGGGATGAAAT
TCCTTACTTGGCGCAAGTCTTTCAGATTATTGATATTTATGATGCGTTAACTAGCGAACGTCCCTACAAAAAAGCTTATA
CTCCAGAAGAAGCATTGTGTATAATGCTGGATGAAACTGAAACGGGATGGCGTAATCCTAAATTGATGCAGCAGTTTGCG
GAGTTTATTCTCTCACACCATAAGGATTCGGGGAGTGCTGAGTAG

Upstream 100 bases:

>100_bases
TAGAATCTAAAACTTTTGAAAAGTCGGCTGGATATATTCGGCTACGCAGTTTGGTTTGAAGTCGGGCGCTATTAATTTTT
TAAACGATTGGGGTACTTCT

Downstream 100 bases:

>100_bases
TGAATGCTGAGTAAGGAATTATCCCTAGTCCCCATGCTCCCTCATTAGCTGGTATGATTTGAGCCTAAATCTCAAAGTAC
CAAGCGCAAAAGCGCTATTT

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MIKRSGDNTTSTEHNVSGSDPPNKLNSISSNRVSGVQNAKDSSLALSKEDFLVSESTQSTTWLTCGINSGYNSLISGTLQ
AQISQVKGFDAESPKVLVVDDHSASRMTAAALLGMEGYAVIEADSGATAIKLVGQQQPDLILLDVMMPEMDGFEVCQLLK
QDEHTRLIPVIFITALNDRRSRIRGIEVGADDFLSKPFDRVELVARVKSLVQQKRLNEDLDHAAQVLFSIASAIESRDPN
TGDHCERLVTLGQAFGEYLTLSRHQIRDLMWGGYLHDIGKVGIPDAVLLKTGKLTPEDWVIMKQHVLIGEKICQPLRSMQ
GVVPIIRHHHERWDGSGYPDGLKGDEIPYLAQVFQIIDIYDALTSERPYKKAYTPEEALCIMLDETETGWRNPKLMQQFA
EFILSHHKDSGSAE

Sequences:

>Translated_414_residues
MIKRSGDNTTSTEHNVSGSDPPNKLNSISSNRVSGVQNAKDSSLALSKEDFLVSESTQSTTWLTCGINSGYNSLISGTLQ
AQISQVKGFDAESPKVLVVDDHSASRMTAAALLGMEGYAVIEADSGATAIKLVGQQQPDLILLDVMMPEMDGFEVCQLLK
QDEHTRLIPVIFITALNDRRSRIRGIEVGADDFLSKPFDRVELVARVKSLVQQKRLNEDLDHAAQVLFSIASAIESRDPN
TGDHCERLVTLGQAFGEYLTLSRHQIRDLMWGGYLHDIGKVGIPDAVLLKTGKLTPEDWVIMKQHVLIGEKICQPLRSMQ
GVVPIIRHHHERWDGSGYPDGLKGDEIPYLAQVFQIIDIYDALTSERPYKKAYTPEEALCIMLDETETGWRNPKLMQQFA
EFILSHHKDSGSAE
>Mature_414_residues
MIKRSGDNTTSTEHNVSGSDPPNKLNSISSNRVSGVQNAKDSSLALSKEDFLVSESTQSTTWLTCGINSGYNSLISGTLQ
AQISQVKGFDAESPKVLVVDDHSASRMTAAALLGMEGYAVIEADSGATAIKLVGQQQPDLILLDVMMPEMDGFEVCQLLK
QDEHTRLIPVIFITALNDRRSRIRGIEVGADDFLSKPFDRVELVARVKSLVQQKRLNEDLDHAAQVLFSIASAIESRDPN
TGDHCERLVTLGQAFGEYLTLSRHQIRDLMWGGYLHDIGKVGIPDAVLLKTGKLTPEDWVIMKQHVLIGEKICQPLRSMQ
GVVPIIRHHHERWDGSGYPDGLKGDEIPYLAQVFQIIDIYDALTSERPYKKAYTPEEALCIMLDETETGWRNPKLMQQFA
EFILSHHKDSGSAE

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=119, Percent_Identity=38.655462184874, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788394, Length=132, Percent_Identity=32.5757575757576, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790860, Length=119, Percent_Identity=38.655462184874, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786784, Length=119, Percent_Identity=34.453781512605, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1787229, Length=171, Percent_Identity=31.5789473684211, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788550, Length=133, Percent_Identity=32.3308270676692, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082012, Length=145, Percent_Identity=33.7931034482759, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790863, Length=122, Percent_Identity=28.6885245901639, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=32.520325203252, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI2367329, Length=124, Percent_Identity=41.1290322580645, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786911, Length=113, Percent_Identity=36.283185840708, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI145693157, Length=108, Percent_Identity=37.962962962963, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1790299, Length=116, Percent_Identity=34.4827586206897, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788713, Length=140, Percent_Identity=32.1428571428571, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790552, Length=119, Percent_Identity=31.0924369747899, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789149, Length=110, Percent_Identity=36.3636363636364, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790437, Length=104, Percent_Identity=38.4615384615385, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 45871; Mature: 45871

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRSGDNTTSTEHNVSGSDPPNKLNSISSNRVSGVQNAKDSSLALSKEDFLVSESTQST
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHCCCCCCCCCCEEECCCCCEEECCCCCC
TWLTCGINSGYNSLISGTLQAQISQVKGFDAESPKVLVVDDHSASRMTAAALLGMEGYAV
EEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEE
IEADSGATAIKLVGQQQPDLILLDVMMPEMDGFEVCQLLKQDEHTRLIPVIFITALNDRR
EECCCCCEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCCHH
SRIRGIEVGADDFLSKPFDRVELVARVKSLVQQKRLNEDLDHAAQVLFSIASAIESRDPN
HHHCEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
TGDHCERLVTLGQAFGEYLTLSRHQIRDLMWGGYLHDIGKVGIPDAVLLKTGKLTPEDWV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCEEEECCCCCCHHHH
IMKQHVLIGEKICQPLRSMQGVVPIIRHHHERWDGSGYPDGLKGDEIPYLAQVFQIIDIY
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
DALTSERPYKKAYTPEEALCIMLDETETGWRNPKLMQQFAEFILSHHKDSGSAE
HHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIKRSGDNTTSTEHNVSGSDPPNKLNSISSNRVSGVQNAKDSSLALSKEDFLVSESTQST
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHCCCCCCCCCCEEECCCCCEEECCCCCC
TWLTCGINSGYNSLISGTLQAQISQVKGFDAESPKVLVVDDHSASRMTAAALLGMEGYAV
EEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEE
IEADSGATAIKLVGQQQPDLILLDVMMPEMDGFEVCQLLKQDEHTRLIPVIFITALNDRR
EECCCCCEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCCHH
SRIRGIEVGADDFLSKPFDRVELVARVKSLVQQKRLNEDLDHAAQVLFSIASAIESRDPN
HHHCEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
TGDHCERLVTLGQAFGEYLTLSRHQIRDLMWGGYLHDIGKVGIPDAVLLKTGKLTPEDWV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCEEEECCCCCCHHHH
IMKQHVLIGEKICQPLRSMQGVVPIIRHHHERWDGSGYPDGLKGDEIPYLAQVFQIIDIY
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
DALTSERPYKKAYTPEEALCIMLDETETGWRNPKLMQQFAEFILSHHKDSGSAE
HHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]